26S proteasome non-ATPase regulatory subunit 10

2007 ◽  
Author(s):  
Keyword(s):  
26S Proteasome ◽  
Regulatory Subunit

Trichoderma reesei prs12 encodes a stress- and unfolded-protein-response-inducible regulatory subunit of the fungal 26S proteasome

1998 ◽  
Vol 33 (4) ◽  
pp. 284-290 ◽  
Author(s):  
Sabine P. Goller ◽  
Markus Gorfer ◽  
C. P. Kubicek
Keyword(s):  
Unfolded Protein Response ◽  
Trichoderma Reesei ◽  
26S Proteasome ◽  
Regulatory Subunit ◽  
Unfolded Protein ◽  
Protein Response

scholarly journals Cloning and Characterization of TaSAP7-A, a Member of the Stress-Associated Protein Family in Common Wheat

2021 ◽  
Vol 12 ◽  
Author(s):  
Wenlu Li ◽  
Yixue Wang ◽  
Runzhi Li ◽  
Xiaoping Chang ◽  
Xiangyang Yuan ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Chlorophyll Content ◽  
Agronomic Traits ◽  
Abiotic Stress Tolerance ◽  
Grain Filling ◽  
26S Proteasome ◽  
Regulatory Subunit ◽  
Germination Stage

Stress association proteins (SAPs) are A20/AN1 zinc-finger domain proteins, which play important roles in plant adaptation to abiotic stress and plant development. The functions of SAPs in some plants were reported, but little is known about it in wheat (Triticum aestivum L.). In this study, we characterized a novel 2AN1-type stress association protein gene TaSAP7-A, which was mapped to chromosome 5A in wheat. Subcellular localization indicated that TaSAP7-A was distributed in the nucleus and cytoplasm. Unlike previously known A20/AN1-type SAP genes, TaSAP7-A was negatively regulated to abiotic stress tolerance. Overexpressing TaSAP7-A Arabidopsis lines were hypersensitive to ABA, osmotic and salt stress at germination stage and post-germination stage. Overexpression of TaSAP7-A Arabidopsis plants accelerated the detached leaves’ chlorophyll degradation. Association analysis of TaSAP7-A haplotypes and agronomic traits showed that Hap-5A-2 was significantly associated with higher chlorophyll content at jointing stage and grain-filling stage. These results jointly revealed that TaSAP7-A is related to the chlorophyll content in the leaves of Arabidopsis and wheat. Both in vivo and in vitro experiments demonstrated that TaSAP7-A interacted with TaS10B, which was the component of regulatory subunit in 26S proteasome. In general, TaSAP7-A was a regulator of chlorophyll content, and favorable haplotypes should be helpful for improving plant chlorophyll content and grain yield of wheat.

Nin1p, a regulatory subunit of the 26S proteasome, is necessary for activation of Cdc28p kinase of Saccharomyces cerevisiae.

1995 ◽  
Vol 14 (13) ◽  
pp. 3105-3115 ◽  
Author(s):  
K. Kominami ◽  
G.N. DeMartino ◽  
C.R. Moomaw ◽  
C.A. Slaughter ◽  
N. Shimbara ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
26S Proteasome ◽  
Regulatory Subunit

A Nonproteolytic Function of the 19S Regulatory Subunit of the 26S Proteasome Is Required for Efficient Activated Transcription by Human RNA Polymerase II†

Biochemistry ◽  
2002 ◽  
Vol 41 (42) ◽  
pp. 12798-12805 ◽  
Author(s):  
Anwarul Ferdous ◽  
Thomas Kodadek ◽  
Stephen Albert Johnston
Keyword(s):  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
26S Proteasome ◽  
Regulatory Subunit

1H, 13C and 15N resonance assignments of Rpn9, a regulatory subunit of 26S proteasome from Saccharomyces cerevisiae

2013 ◽  
Vol 8 (2) ◽  
pp. 307-311 ◽  
Author(s):  
Yujie Wu ◽  
Yunfei Hu ◽  
Changwen Jin
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Resonance Assignments ◽  
26S Proteasome ◽  
Regulatory Subunit

CADp44: a novel regulatory subunit of the 26S proteasome and the mammalian homolog of yeast Sug2p

Gene ◽  
1996 ◽  
Vol 181 (1-2) ◽  
pp. 63-69 ◽  
Author(s):  
Vernon W. Bauer ◽  
Jonathan C. Swaffield ◽  
Stephen Albert Johnston ◽  
Matthew T. Andrews
Keyword(s):  
26S Proteasome ◽  
Regulatory Subunit

scholarly journals The yeast SEN3 gene encodes a regulatory subunit of the 26S proteasome complex required for ubiquitin-dependent protein degradation in vivo.

1995 ◽  
Vol 15 (11) ◽  
pp. 6311-6321 ◽  
Author(s):  
D J DeMarini ◽  
F R Papa ◽  
S Swaminathan ◽  
D Ursic ◽  
T P Rasmussen ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Genetic Selection ◽  
26S Proteasome ◽  
Yeast Cells ◽  
Regulatory Subunit ◽  
20S Proteasome ◽  
Amino Terminal ◽  
A Subunit

The yeast Sen1 protein was discovered by virtue of its role in tRNA splicing in vitro. To help determine the role of Sen1 in vivo, we attempted to overexpress the protein in yeast cells. However, cells with a high-copy SEN1-bearing plasmid, although expressing elevated amounts of SEN1 mRNA, show little increase in the level of the encoded protein, indicating that a posttranscriptional mechanism limits SEN1 expression. This control depends on an amino-terminal element of Sen1. Using a genetic selection for mutants with increased expression of Sen1-derived fusion proteins, we identified mutations in a novel gene, designated SEN3. SEN3 is essential and encodes a 945-residue protein with sequence similarity to a subunit of an activator of the 20S proteasome from bovine erythrocytes, called PA700. Earlier work indicated that the 20S proteasome associates with a multisubunit regulatory factor, resulting in a 26S proteasome complex that degrades substrates of the ubiquitin system. Mutant sen3-1 cells have severe defects in the degradation of such substrates and accumulate ubiquitin-protein conjugates. Most importantly, we show biochemically that Sen3 is a subunit of the 26S proteasome. These data provide evidence for the involvement of the 26S proteasome in the degradation of ubiquitinated proteins in vivo and for a close relationship between PA700 and the regulatory complexes within the 26S proteasome, and they directly demonstrate that Sen3 is a component of the yeast 26S proteasome.

scholarly journals Dissection of Maize Drought Tolerance at the Flowering Stage Using Ge-Nome-Wide Association Study

2021 ◽  
Author(s):  
Siffat Ullah Khan ◽  
Yanxiao Zheng ◽  
Zaid Chachar ◽  
Xuhuan Zhang ◽  
Guyi Zhou ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Candidate Genes ◽  
Pyridoxal Phosphate ◽  
Association Studies ◽  
26S Proteasome ◽  
Regulatory Subunit ◽  
Tolerance Index ◽  
Genome Wide Association Studies ◽  
Flowering Stage

Abstract Drought is one of the most critical environmental factors constraining corn production especially when it occurs during flowering, resulting in serious yield losses. In this study, anthesis to silk interval (ASI), plant height (PH), and ear biomass at the silking date (EBM) of 279 inbred lines were evaluated under water-stress (WS) and well-water (WW) field conditions for three consecutive years. Averagely, ASI was extended by 25.96%, ear biomass was decreased by 17.54%, and the PH was reduced by 12.47% under drought stress conditions. Genome wide association studies (GWAS) were carried out using phenotypic values under WS, WW and drought-tolerance index (WS-WW or WS/WW) applying mixed linear model controlling both population structure and relative kinship. Totally, 71, 159, and 21 SNPs were significantly (P < 10-5) associated with ASI, ear biomass, and PH, respectively. Candidate genes encoding ARABIDILLO 1 protein, glycoprotein, Tic22-like and Zinc finger family protein for ASI, and 26S proteasome non-ATPase regulatory subunit-9 for EBM, were identified under both WW and WS conditions. Pyridoxal phosphate transferase was associated with EBM under drought stress treatment in consecutive two years. Furthermore, most candidate genes were evidenced to be drought responsive in the association panel. Meanwhile, the favourable/drought tolerance haplotypes were identified based on haplotype analysis. These findings provide insights into the genetic basis of drought tolerance at the flowering stage especially for the female inflorescence development and will facilitate high drought tolerant maize breeding.

scholarly journals Transient expression of 'Candidatus Liberibacter asiaticus' effector SDE1 induces chlorosis by suppressing NbDDX3 gene in Nicotiana benthamiana

2020 ◽  
Author(s):  
Yinghui Zhou ◽  
Yanjiao Li ◽  
Zhiqin Liu ◽  
Xuxu Zhao ◽  
Xianyuan Xu ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Transient Expression ◽  
Nicotiana Benthamiana ◽  
Hypothetical Protein ◽  
26S Proteasome ◽  
Bimolecular Fluorescence Complementation ◽  
Host Responses ◽  
Regulatory Subunit ◽  
Suppression Effect ◽  
Liberibacter Asiaticus

Abstract Background: 'Candidatus Liberibacter asiaticus' (Las) is the pathogenic bacterium that causes Huanglongbing in citrus plants, as well as in several types of experimental plants. Las releases a set of effectors to modulate host responses. One of these critical effectors is Sec-delivered effector 1 (SDE1), which induces chlorosis in Nicotiana benthamiana. Results: Four SDE1-interacting proteins were identified from N. benthamiana, including DEAD-box RNA helicase DDX3, 26S proteasome non-ATPase regulatory subunit PSMD14, an ARM repeat protein, and a hypothetical protein. Gene silencing revealed that knockdown of the NbDDX3 gene led to chlorosis in N. benthamiana leaves. Fluorescent signal detection revealed that SDE1 was localized to the cell membrane, cytoplasm, and nucleus. Simultaneously, NbDD3 was expressed in cytoplasmic vesicles, as well as in the cell membrane. The interactions between SDE1 and NbDDX3 were shown to be localized on cell membrane using co-localization and bimolecular fluorescence complementation analysis. Moreover, the transcription of NbDDX3 gene was substantially suppressed in N. benthamiana plants that expressed SDE1. Conclusion: Las effector SDE1 interacts with NbDDX3 at the cell membrane. Most importantly, the transient expression of SDE1 exerts a suppression effect on the transcription of NbDDX3 gene. The silencing of NbDDX3 leads to leaf chlorosis in N. benthamiana. This provided evidence to understand the molecular events in association with chlorosis induced by SDE1.

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