scholarly journals Comparative ultrastructure of skeletal muscles of broiler chickens and quails

Author(s):  
Ulyana Kundryukova ◽  
Ludmila Drozdova ◽  
Yakov Beikin ◽  
Svetlana Pichugova
2000 ◽  
Vol 130 (4) ◽  
pp. 813-819 ◽  
Author(s):  
Soraya Temim ◽  
Anne-Marie Chagneau ◽  
Rosaria Peresson ◽  
Sophie Tesseraud

2016 ◽  
Vol 91 (5) ◽  
pp. 633-636 ◽  
Author(s):  
M. Zibaei ◽  
S.M. Sadjjadi ◽  
S. Maraghi

AbstractConsuming raw and undercooked meat is known to enhance the risk of human toxocariasis because Toxocara species have a wide range of paratenic hosts, including chickens. The aim of this study was to identify species of Toxocara in naturally infected broiler chickens using molecular approaches. A polymerase chain reaction (PCR) method was used for the differentiation of Toxocara canis and Toxocara cati larvae recovered from tissues and organs, and identified by microscopic observations. Thirty-three 35- to 47-day-old broiler chickens were used for examination of Toxocara larvae. The duodenum, liver, lungs, heart, kidneys, skeletal muscles and brain of each chicken were examined using the pepsin method, and DNA from each tissue was extracted as the template for PCR assay. The findings revealed that 5 of 33 (15.2%) broiler chickens were infected with Toxocara larvae. Larvae were recovered from the liver (n = 19), duodenum (n = 8), skeletal muscles (n = 8) and brain (n = 2) of broiler chickens naturally infected with Toxocara spp. The results showed that the frequencies of the species in the chickens were T. canis larvae (n = 5, 83.3%) and T. cati larvae (n = 1, 16.7%). Our data from the present study demonstrated the importance of broiler chickens as a paratenic host for the parasite's life cycle in the environment. The implementation of DNA amplification as a routine diagnostic technique is a specific and alternative method for identification of Toxocara larvae, and allowed the observation of specific species under field conditions within the locations where broiler chickens are typically raised and exposed to Toxocara spp. eggs or larvae.


Author(s):  
O. I. Vishchur ◽  
L. V. Romanovych ◽  
K. B. Smolyaninov ◽  
M. B. Masyuk ◽  
М. М. Romanovych

The purpose of the work was to find out the changes in the lipid composition of the liver and skeletal muscles of broiler chickens at different stages of growth under the influence of supplements of vitamins E and C in their diet. The studies were conducted on four groups of broiler chickens. The control group was fed a standard compound feed. The first experimental group in addition to the specified compound feed received tocopherol acetate in the amount of 0.1 g/kg, the second — ascorbic acid, 0.25 g/kg. The third experimental group of chickens received tocopherol acetate and ascorbic acid at mentioned doses. The lipids from these tissues were extracted by the Folch method, and the ratio of individual lipid classes was determined by thin layer chromatography on silica gel. The results of the studies indicate the stimulating effect of vitamins E and C on the accumulation of total lipids in the skeletal muscles and liver of broiler chickens. In addition, we can conclude that feeding the broiler chickens of supplements containing vitamins E and C during the period of intensive growth promotes the increase of lipid synthesis in their liver and deposition of synthesized lipids in skeletal muscle. However, these processes are accompanied by a redistribution of the ratio of individual lipid classes in the investigated organs and tissues of chickens


2020 ◽  
pp. 19-27
Author(s):  
В. М. Кириченко ◽  
І. В. Яценко ◽  
Л. В. Бусол ◽  
В. Я Бінкевич ◽  
Я. К. Сердюков

The work is devoted to the study of the microscopic structure of broiler chickens organs for the enrichment of the diet with a complex of nano trace elements which include the citrates of nano Zn, Cu, Ag, Co, He, Mg. It was found that the use of a complex of nano-micronutrients, at a dose of 1 cm3/dm3 of drinking water in the drinking mode for five days in a row after five days, did not cause pathomorphological changes in the internal organs and skeletal muscles of slaughtered broiler chickens.The use in the diet of the experimental bird complex of nano trace elements at a dose of 10 and 20 cm3/dm3 drinking water can lead to structural disorders in the liver, kidneys and spleen in the form of local circulatory disorders, necrosis of cells of the parenchyma of organs. Extracapillary glomerulonephritis and necrotic- dystrophic changes in the epithelium of the tubules are recorded in the kidneys (proteinaceous nephrosis by type of granular dystrophy, in some cases necrotic nephrosis); In internal organs there are local circulatory disorders (congestive hyperemia, stasis, etc.). In cells of parenchyma of organs destruction of organelles, cell membranes is registered, that causes at first development of dystrophic processes on decompositive type, and subsequently - death of cells.


Author(s):  
D. A. Fischman ◽  
J. E. Dennis ◽  
T. Obinata ◽  
H. Takano-Ohmuro

C-protein is a 150 kDa protein found within the A bands of all vertebrate cross-striated muscles. By immunoelectron microscopy, it has been demonstrated that C-protein is distributed along a series of 7-9 transverse stripes in the medial, cross-bridge bearing zone of each A band. This zone is now termed the C-zone of the sarcomere. Interest in this protein has been sparked by its striking distribution in the sarcomere: the transverse repeat between C-protein stripes is 43 nm, almost exactly 3 times the 14.3 nm axial repeat of myosin cross-bridges along the thick filaments. The precise packing of C-protein in the thick filament is still unknown. It is the only sarcomeric protein which binds to both myosin and actin, and the actin-binding is Ca-sensitive. In cardiac and slow, but not fast, skeletal muscles C-protein is phosphorylated. Amino acid composition suggests a protein of little or no αhelical content. Variant forms (isoforms) of C-protein have been identified in cardiac, slow and embryonic muscles.


Author(s):  
F.T. Llados ◽  
V. Krlho ◽  
G.D. Pappas

It Is known that Ca++ enters the muscle fiber at the junctional area during the action of the neurotransmitter, acetylcholine (ACh). Pappas and Rose demonstrated that following Intense stimulation, calcium deposits are found In the postsynaptic muscle membrane, Indicating the existence of calcium uptake In the postsynaptic area following ACh release. In addition to this calcium uptake, when mammal Ian skeletal muscles are exposed to a sustained action of the neurotransmitter, muscle damage develops. These same effects, l.e., Increased transmitter release, calcium uptake and finally muscle damage, can be obtained by Incubating the muscle with lonophore A23178.


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