scholarly journals The role of Biofilm for Pseudomonas aeruginosa to resistance ultraviolet and MIC concentration for antibiotics

2012 ◽  
Vol 36 (0A) ◽  
pp. 13-19
Author(s):  
احمد محمد تركي
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
High Resistance ◽  
The Other ◽  
Bacterial Isolates ◽  
Staph Aureus ◽  
E Coli ◽  
The Impact ◽  
And Staphylococcus Aureus

The present study is conducted to in restigate the bacteria Pseudomonas aeruginosa, the impact of ultraviolet on the bacterial isolates under study and the resistance of these isolates to ultraviolet are studied in comparison to two standard isolates ( E . coli and Staphylococcus aureus ) which are considered sensitive to ultraviolet . The natures of the resistance of the isolates, under study, are also being investigated against the different antibiotics. The isolates are subjected to a test to examine their sensitivity to (12) types of antibiotics used routinely in the treatment of various infection of these bacteria. They are (streptomycin , cephalothin ,Gentamycin , cefotaxime ,nitrofurantion ,ampicillin, amoxicillin, rifampin, lincomycin, tetracycline, erythromycin and ciprofloxacin ).The lowest concentration installer ( MIC ) is also testified in accordance with six types of antibiotics (streptomycin, cefotaxime , rifampin , nitrofurantion , Gentamycin , amoxicillin ).The biologic effectiveness of the overlap between the bacterial isolates , under study, is examined against four bacteria (klebseilla pneumonia , Staphylococcus aureus , Enterobacter , Proteus ) The result of using the ultraviolet with different wavelength show the ability of the five local isolates used to resistance of ultraviolet reaching (180 s.) in comparison to the isolates E.coli and staph. aureus in which the ratio of killing is %100 at a time of exposing 40 , 60 sec. respectively. The results indicated that the five local bacterial isolates have high resistance to the most tested antibiotics, It is shouted that all of them have resistance to (erythromycin , tetracycline , lincomycin , Gentamycin ) but they are sensitive towards antibiotic streptomycin . as for the other antibiotics , over can find that the isolates are varied of them for being resisting or sensitive towards them .The results of testing the inhabited effectiveness of the five bacterial isolates towards some other bacterial isolates show the efficiency of the five local isolates in the inhabitation of growth of the five studied bacterial isolates.

Antibacterial and antibiofilm activity of the bacteriocin-producing strain Leuconostoc mesenteroides CHBY46 isolated from Algerian dromedary milk against Pseudomonas aeruginosa and Staphylococcus aureus biofilms

2019 ◽  
Vol 8 (4) ◽  
pp. 120-131
Author(s):  
Yahia Bellil ◽  
Zineb Benmechernene ◽  
Wassila Chahrour Bellil ◽  
Mebrouk Kihal
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
16S Rrna Gene Sequencing ◽  
Antibiofilm Activity ◽  
Rrna Gene ◽  
Biofilm Inhibition ◽  
Staph Aureus ◽  
Spoilage Bacteria ◽  
Dromedary Milk ◽  
And Staphylococcus Aureus

In order to control biofilm formation of pathogenic and spoilage bacteria in foods, some species of Leuconostoc are very important in food industries, as they increase the shelf life of foods during preservation. In this study the strain CHBY46 a bacteriocin-producing strain belonging to Leuconostoc ge-nus isolated from dromedary milk in the south of Algeria was characterized by 16S rRNA gene sequencing and MALDITOF MS mass spectrometry, tested for its antibacterial and antibiofilm activities against Pseudomonas aeruginosa and Staphylococcus aureus. The produced bacteriocin was partially puri-fied with sulfate ammonium precipitation and RP-HPLC. The strain CHBY46 was classified as Leuc. mesenteroides after molecular identification. Among the bacteria tested the pathogens Staph. aureus ATCC 29213 and Ps. aeru-ginosa ATCC 27653 were sensitive to this bacteriocin with 480 AU/ml. Antibi-ofilm activity was investigated by crystal violet assay. The bacteriocin of Leuc. mesenteroides CHBY 46 exhibited significant biofilm inhibition ; 35.58% with Ps. aeruginosa, and 42.11% with Staph. aureus. Tricine-SDS-PAGE analysis of the partially purified bacteriocin indicated a low molecular weight of approximately 3.5 kDa. Therfore, we conclude that bacteriocins from Leuco-nostoc have the potential as a therapeutic strategy against pathogen’s bio-films, which contribute, to bacterial pathogenicity and resistance toward antibiotics or being used in foods as adjunsts to contribute food safety.

scholarly journals Isolation and Identification of Microorganisms Growing on the Root of Leguminous Plants (Groundnut, Soya bean and Pea)

2019 ◽  
pp. 1-7
Author(s):  
Bukola Catherine Akin-Osanaiye ◽  
Oluwatobi Olaife Arowolo ◽  
Ifeyomi Wilfred Olobayotan
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Soya Bean ◽  
Leguminous Plant ◽  
Isolation And Identification ◽  
Leguminous Plants ◽  
And Staphylococcus Aureus

Study on the isolation and identifications of bacteria associated with the root of legumes were conducted using Spread Plate Technique. The frequencies of occurrences of the bacteria isolate showed that a total of sixteen (16) bacteria belonging to three genera and four species were isolated from the leguminous plants. Maximum number recovered from sample collected from the root of groundnut was seven (7) followed by Soya bean with five (5) while Pea recorded the least number of four (4). Role of Bacillus subtilis in the soil around the leguminous plant was the highest, which covered about 37.50% of the total isolates. Other bacteria that were also isolated from the soil around the legumes root include Bacillus cereus and Staphylococcus aureus which covered about four (4) each representing 25.0% of the total isolates while Pseudomonas aeruginosa recorded the least value of 12.50%. The bacteria isolated from the root of the legumes were not significantly different (P < 0.05). The bacteria have Nitrogen-fixing potential, having isolated from three leguminous plants which include Soya bean, Groundnut and Pea.

scholarly journals Preparation of Ethanol-Free Hand Sanitizers Gels and Studying its Sterile Efficacy

2021 ◽  
Vol 2063 (1) ◽  
pp. 012007
Author(s):  
Mahmood M. Fahad ◽  
Mohammed G.A. Al-Khuzaie
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Candida Albicans ◽  
Aloe Vera ◽  
Tea Tree Oil ◽  
Tea Tree ◽  
E Coli ◽  
Hand Sanitizer ◽  
Thickening Agents ◽  
And Staphylococcus Aureus

Abstract Due to the spread of the Corona pandemic, the demand for hand sanitizers has increased dramatically. This led to a global scarcity of sterilizers. Therefore, this study was performed to produce and evaluate the effectiveness of new commercially available, cheap and ethanol-free hand sanitizers from various sources. The new hand sanitizers were prepared by mixing (glycerin) as a moisturizer and (carbomer) as thickening agents to form the gel with (tri-ethanolamine base) as pH neutralizer and (Tea tree oil, Aloe vera extract or Povidone 10%) respectively as antiseptic agents. All prepared hand sanitizers were examined with four kinds of bacteria (Enterococcus faecalis, E. coli, Pseudomonas aeruginosa and Staphylococcus aureus) And two kinds of fungi (Candida albicans and Aspergillus flavus). The prepared hand sanitizer gels showed good sterilization efficacy and similar to commercially prepared hand sanitizers (70% ethanol).

Antimicrobial Activity of Leaves Extracts of Tamarindus indica L. (Magyee)

2019 ◽  
Vol 31 (2) ◽  
pp. 125-130
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Petroleum Ether ◽  
Aqueous Extracts ◽  
Tamarindus Indica ◽  
E Coli ◽  
Ethanolic Extracts ◽  
And Staphylococcus Aureus

This study aimed to determine antimicrobial activity of extracts of Tamarindus indica L. leaves (Magyee). It was the laboratory-based quasi experimental study design. Study sites were the University of Pharmacy (Mandalay and Yangon) and the Department of Medical Research (Yangon). The leaves of Tamarindus indica L. were extracted with petroleum ether, ethanol and distilled water by successive Soxhlet extraction method. Physicochemical and phytochemical studies were conducted according to reference analytical procedures. In vitro antimicrobial activity of three extracts of Tamarindus indica L. leaves were studied on control strains of bacteria (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and Klebsiella pneumoniae) by agar disc diffusion method. Phytochemical analysis of Tamarindus indica L. leaves showed the presence of alkaloids, amino acids, carbohydrates, flavonoids, glycosides, polyphenol, starches, steroids/terpenoids, tannins, reducing sugars and anthroquinone. Ethanolic and aqueous extracts exhibited significant antibacterial activities against Pseudomonas aeruginosa and Staphylococcus aureus. Both aqueous and ethanolic extracts possessed promising antibacterial activity on Pseudomonas aeruginosa and Staphylococcus aureus. This was an important finding upon pathogenic organisms causing food-borne diseases, skin and soft tissue infection. The petroleum ether extract did not show antibacterial activity against all tested organisms. With ethanolic extracts, the minimum inhibitory concentrations (MIC) results were 6.25 mg/ml for P. aeruginosa, S. aureus and 25 mg/ml for E. coli. For aqueous extracts, the MIC of P. aeruginosa and S. aureus were 12.5 mg/ml and K. pneumoniae was 25 mg/ml, respectively. Oral acute administration of 5000 mg/kg mice body weight of ethanolic and aqueous extracts of Tamarindus indica L. leaves resulted in no mortality. Therefore, the crude extracts from Tamarindus indica L. leaves had antimicrobial activity against K. pneumoniae, P. aeruginosa, S. aureus and E. coli.

scholarly journals Specificity in the Susceptibilities of Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus Clinical Isolates to Six Metal Antimicrobials

Antibiotics ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 51 ◽  
Author(s):  
Natalie Gugala ◽  
Dennis Vu ◽  
Michael D. Parkins ◽  
Raymond J. Turner
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Great Variability ◽  
Clinical Isolates ◽  
Minimal Inhibitory Concentrations ◽  
E Coli ◽  
And Staphylococcus Aureus ◽  
Degree Of Similarity ◽  
Comparative Information

In response to the occurrence of antibiotic resistance, there has been rapid developments in the field of metal-based antimicrobials. Although it is largely assumed that metals provide broad-spectrum microbial efficacy, studies have shown that this is not always the case. Therefore, in this study, we compared the susceptibilities of 93 clinical isolates belonging to the species Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus against six metals, namely aluminum, copper, gallium, nickel, silver and zinc. To provide qualitative comparative information, the resulting zones of growth inhibition were compared to the minimal inhibitory concentrations of three indicator strains E. coli ATCC 25922, P. aeruginosa ATCC 27853 and S. aureus ATCC 25923. Here, we demonstrate that the metal efficacies were species- and isolate-specific. Only several isolates were either resistant or sensitive to all of the six metals, with great variability found between isolates. However, the greatest degree of similarity was found with the E. coli isolates. In contrast, the susceptibilities of the remaining two collections, S. aureus and P. aeruginosa, were more highly dispersed. Using this information, we have shown that metals are not equal in their efficacies. Hence, their use should be tailored against a particular microorganism and care should be taken to ensure the use of the correct concentration.

Role of topological scale in the differential fouling of Pseudomonas aeruginosa and Staphylococcus aureus bacterial cells on wrinkled gold-coated polystyrene surfaces

Nanoscale ◽  
2018 ◽  
Vol 10 (11) ◽  
pp. 5089-5096 ◽  
Author(s):  
Duy H. K. Nguyen ◽  
Vy T. H. Pham ◽  
Vi Khanh Truong ◽  
Igor Sbarski ◽  
James Wang ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Surface Area ◽  
Bacterial Cells ◽  
Planar Space ◽  
Surface Features ◽  
Extensive Surface ◽  
And Staphylococcus Aureus

Wrinkled patterns, which possess an extensive surface area over a limited planar space, can provide surface features ranging across the nano- and microscale.

scholarly journals RESISTANCE OF THE MICROORGANISMS ISOLATED FROM SURGICAL HOSPITAL ENVIRONMENT TO DISINFECTANTS

2016 ◽  
Vol 1 (3) ◽  
pp. 55-59
Author(s):  
Анганова ◽  
Elena Anganova ◽  
Крюкова ◽  
Natalya Kryukova
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Fungicidal Activity ◽  
District Hospital ◽  
The Other ◽  
Hospital Environment ◽  
E Coli ◽  
Surgical Ward ◽  
Central District

The article presents the results of the study of resistance of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Aspergillus fungi to disinfectants. The microorganisms were isolated from the surgical ward environment of Central District Hospital of Neryungri. All strains were sensitive to Lysoformin 3000. Most of the microorganisms were sensitive to Ecocide and Septodor (85,7 % and 71,4 % respectively). Only E. coli was resistant to these disinfectants. The microorganisms showed the highest resistance to Javelion. S. aureus and E. coli were resistant to Javelion. The most effective disinfectants against Aspergillus were 1% solution of Septabic, 0,2% solution of Vegasept, 0,4% solution of Septodor, 0,5% solution of Lysoformin 3000. The other disinfectants (Chlor-sept, Hypocloride and Alfadez forte) had lower fungicidal activity against Aspergillus.

scholarly journals Antibacterial Effects of Thuja Leaves Extract

2017 ◽  
Vol 5 (2) ◽  
pp. 256-260 ◽  
Author(s):  
Shiv Nandan Sah ◽  
Sunil Regmi ◽  
Man Kumar Tamang
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Soxhlet Extraction ◽  
Disc Diffusion ◽  
Antibacterial Effects ◽  
E Coli ◽  
Diffusion Technique ◽  
And Staphylococcus Aureus

Various medicinal plants are common in use in Nepal for the treatment of different diseases. Nowadays, drug resistance has emerged as a major problem for various infections, in such case plants can be used as alternative for the production of new antimicrobial agents. Thuja (in Nepali: dhupi) is a small evergreen genus of the Cupressaceae family. This species is widely cultivated as a common ornamental plant in Nepal and India. This study examined the antibacterial activity of Thuja leaves extract on gram positive (Staphylococcus aureus and Streptococcus spp.) and gram-negative bacteria (E. coli and Pseudomonas aeruginosa). Thuja leaves were collected from different localities of Dharan, and dried under shade for 10 days. They were then grinded using mechanical grinder. Leaf extract (oleoresin) was obtained by soxhlet extraction technique using mixture of Ethyl acetate, Ethanol and chloroform in the ratio 40:30:30 as the solvent. The antibacterial activity of Thuja oleoresin was tested using both Agar well diffusion as well as disc diffusion technique. Minimum Inhibitory Concentration(MIC) was  determined by agar well diffusion on MHA plates. Thuja oleoresin showed distinct antibacterial activity towards all four isolates on both agar well and disc diffusion methods. MIC for Pseudomonas aeruginosa and Streptococcus spp. was found to be 12.5 µl whereas for E. coli and Staphylococcus aureus, MIC was 25 µl. Thus from our results, it is concluded that Thuja leaves have antibacterial effects and can be a potential source for production of antibacterial drugs.Int. J. Appl. Sci. Biotechnol. Vol 5(2): 256-260

scholarly journals How Bacterial Adaptation to Cystic Fibrosis Environment Shapes Interactions Between Pseudomonas aeruginosa and Staphylococcus aureus

2021 ◽  
Vol 12 ◽  
Author(s):  
Laura Camus ◽  
Paul Briaud ◽  
François Vandenesch ◽  
Karen Moreau
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Chronic Infection ◽  
Bacterial Strains ◽  
Bacterial Adaptation ◽  
Long Time ◽  
The Subject ◽  
The Impact ◽  
And Staphylococcus Aureus

Pseudomonas aeruginosa and Staphylococcus aureus are the two most prevalent bacteria species in the lungs of cystic fibrosis (CF) patients and are associated with poor clinical outcomes. Co-infection by the two species is a frequent situation that promotes their interaction. The ability of P. aeruginosa to outperform S. aureus has been widely described, and this competitive interaction was, for a long time, the only one considered. More recently, several studies have described that the two species are able to coexist. This change in relationship is linked to the evolution of bacterial strains in the lungs. This review attempts to decipher how bacterial adaptation to the CF environment can induce a change in the type of interaction and promote coexisting interaction between P. aeruginosa and S. aureus. The impact of coexistence on the establishment and maintenance of a chronic infection will also be presented, by considering the latest research on the subject.

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