scholarly journals Evaluation of the anti-inflammatory activity of a gel based on Afzelia africana (fabaceae) leaves

2021 ◽  
Vol 16 (3) ◽  
pp. 041-048
Author(s):  
Pondy Bias Mathilde Audrey ◽  
Djoko Ernest ◽  
Foutse Yimta Wandji ◽  
Wona Jean Pierre ◽  
Wouessidjewe Denis
Keyword(s):  
Leaf Extract ◽  
Ethanolic Extract ◽  
Traditional Healers ◽  
Inflammatory Activity ◽  
Hydroxyethyl Cellulose ◽  
Bark Extract ◽  
Onset Of Action ◽  
Topical Gel ◽  
Anti Inflammatory ◽  
Afzelia Africana

Afzelia africana is a plant used by traditional healers in the treatment of several inflammatory pathologies, in particular edema, rheumatism, lumbago. The effectiveness of its barks in treating inflammation is well established. Ethnobotanical data led researchers to formulate a stable topical hydrogel based on bark extract, the tolerance and effectiveness of which in animals have been found to be satisfactory. As excessive use of the bark may impair the survival of the plant, further leaf studies have been performed and the anti-inflammatory effect has been confirmed in rats. The objective of the present study was to evaluate the anti-inflammatory efficacy of a topical gel based on the hydro-ethanolic extract of these leaves in patients with inflammatory symptoms, compared to a diclofenac gel. Using hydroxyethyl cellulose and glycerol a stable gel was produced and used for the evaluation of the anti-inflammatory activity of the leaves of the plant. The dry extract obtained from the leaves was rich in flavonoids (57.26 mg quercetin equivalent per gram), phenols, tannins, alkaloids, anthraquinones and steroids. We carried out a randomized pilot study on 52 patients who came for consultation at the « Cliniques Universitaires des Montagnes » and at the Bangangté District Hospital for inflammatory symptoms between June 1 and July 17, 2020; pregnant women were excluded from the study. Topical administration on 15 inflammatory pathologies revealed that the Afzelia africana leaf extract gel exhibits a better onset of action (4.29 ± 1.89 min) than the diclofenac gel (16.96 ± 4, 73 min). The decrease in pain visual analogue scale (VAS): values ​​for Afzelia africana gel was greater than for diclofenac gel, with P < 0.05 on day 2 and P < 0.001 from day 3 to day 7. The difference in First Hour Sedimentation Rate was significant between the two gels (P < 0.05); but this difference was no longer statistically significant after the second hour (P ˃ 0.05). In addition, Afzelia africana leaf extract gel reduced swelling (P = 0.001) and stiffness (P < 0.001) better than diclofenac gel. These results clarify the effectiveness of Afzelia africana leaf extract gel in the management of inflammatory pathologies and / or symptoms.

Pharmacological Evaluation of Antipyretic, Analgesic and Anti-inflammatory Activities of Ethanolic Extract of Cassia fistula

2021 ◽  
Author(s):  
D.K. Sharma ◽  
S.K. Sharma ◽  
M.K. Lonare ◽  
Rajdeep Kaur ◽  
V.K. Dumka
Keyword(s):  
Analgesic Activity ◽  
Ethanolic Extract ◽  
Inflammatory Activity ◽  
Bark Extract ◽  
Control Group ◽  
Albino Rats ◽  
Hot Plate ◽  
Antipyretic Activity ◽  
Tail Immersion ◽  
Anti Inflammatory

Background: The antipyretic, analgesic and anti-inflammatory activities of two concentrations (100 and 200 mg/kg) of ethanolic extract of leaf, bark, flower and fruit pulp of C. fistula were determined in male wistar albino rats. Methods: Antipyretic activity was assessed by E. coli endotoxin induced pyrexia. Analgesic activity was assessed by hot plate, tail immersion and acetic acid induced writhing test. Anti-inflammatory activity was evaluated by carrageenan-induced rat paw edema assay. Result: Significant (p less than 0.05) antipyretic activity was exhibited from 2h onwards by bark extract @ 200 mg/kg and from 3h onwards by bark extract @100 mg/kg and leaves extract @ 200mg/kg as compared to control group. Significant (p less than 0.05) analgesic activity was shown by extract of bark @ 200 mg/kg as it is evident by increase in reflex time in hot plate (90,120,180 min), tail immersion test (120,180 min) and inhibition of writhing (32.12%). Significant (p less than 0.05) anti-inflammatory activity was exhibited from 3h post administration by bark @ 200 and leaves @ 100 and 200 mg/kg.

Antibacterial and Anti-Inflammatory Activity of the Silver Nano Particles Synthesized from the Methanolic Leaf Extract of Litsea quinqueflora (Dennst.) Suresh

2020 ◽  
Vol 12 (4) ◽  
pp. 443-449
Author(s):  
Sumin Mary Jose ◽  
M. Anilkumar
Keyword(s):  
Leaf Extract ◽  
Protein Denaturation ◽  
Colour Change ◽  
Traditional Healers ◽  
Nano Particles ◽  
Inflammatory Activity ◽  
Uv Spectrum ◽  
X Ray ◽  
Vis Spectroscopy ◽  
Anti Inflammatory

Litsea quinqueflora (Dennst.) Suresh is an ethno botanically important medicinal plant used by the traditional healers of Kerala to heal inflammatory disorders. In this paper we describe the synthesis of bio-nanoparticles of silver, capped with the methanolic leaf extract L. quinqueflora; its characterization and anti-bacterial and anti-inflammatory activities. The production of silver nanoparticles was recognized by the colour change of the solution from yellow to dark brown; it was further confirmed and characterized by UV-Vis spectroscopy, Fourier Transform Infrared (FTIR) spectroscopy, X-ray Diffraction (XRD) studies, Scanning Electron Microscopy (SEM) and Energy Dispersive X-ray Analysis (EDAX). The Surface Plasmon Resonance (SPR) peak obtained at 421 nm from the UV spectrum confirms the formation of AgNPs. The capping of nanoparticles with phytochemicals was assessed from the FTIR spectrum. Also, XRD, SEM and EDAX analysis showed that the nanoparticles are crystalline in nature with an average size of 62.63 nm. The AgNPs thus synthesized exhibited anti-bacterial activity against Pseudomonas fluorescens, Staphylococcus warneri, Klebsiella pneumoniae, Bacillus cereus and Escherichia coli. The anti inflammatory activity of the nanoparticles was tested by inhibition of protein denaturation assay; this gave positive results with the IC 50 value of 244.92 μg/ml.

scholarly journals Analgesic and anti-Inflammatory activity of Tradescantia fluminensis leaves extract

2017 ◽  
Vol 6 (1) ◽  
pp. 34-37
Author(s):  
Wilson Rwai Waweru ◽  
◽  
Lawrence Obado Osuwat ◽  
Connie Wambui Mureithi ◽  
◽  
...  
Keyword(s):  
Leaf Extract ◽  
Ethanolic Extract ◽  
Inflammatory Activity ◽  
Control Group ◽  
Albino Rats ◽  
Crude Leaf Extract ◽  
Tradescantia Fluminensis ◽  
Anti Inflammatory ◽  
Analgesic Activities ◽  
Wistar Albino Rats

Objective: The present study sought to evaluate the anti-inflammatory and analgesic activities of ethanolic extract from Tradescantia fluminensis leaves. Methods: The crude leaf extract of T. fluminensis was investigated for anti-inflammatory and analgesic activities in Wistar Albino rats and mice respectively. The anti-inflammatory activity was investigated using egg albumin induced oedema model while acetic acid and formalin-induced paw licking models were used to evaluate the analgesic activity. Results: The extract showed a significant (P<0.05) dose-dependent inhibition of inflammation and pain compared to the control group. Conclusion: The leaf extract possesses anti-inflammatory and analgesic activities. The activities could be attributed to phytochemicals present in the plant.

scholarly journals Phytochemical Analysis, Antioxidant and Anti-Inflammatory Activity of Calyces from Physalis peruviana

2014 ◽  
Vol 9 (11) ◽  
pp. 1934578X1400901 ◽  
Author(s):  
Reina M. Toro ◽  
Diana M. Aragón ◽  
Luis F. Ospina ◽  
Freddy A. Ramos ◽  
Leonardo Castellanos
Keyword(s):  
Antioxidant Activities ◽  
Folk Medicine ◽  
Ethanolic Extract ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Physalis Peruviana ◽  
Edema Model ◽  
Anti Inflammatory ◽  
Bio Assay

Physalis peruviana calyces are used extensively in folk medicine. The crude ethanolic extract and some fractions of calyces were evaluated in order to explore antioxidant and anti-inflammatory activities. The anti-inflammatory activity was evaluated by the TPA-induced ear edema model. The antioxidant in vitro activity was measured by means of the superoxide and nitric oxide scavenging activity of the extracts and fractions. The butanolic fraction was found to be promising due to its anti-inflammatory and antioxidant activities. Therefore, a bio-assay guided approach was employed to isolate and identify rutin (1) and nicotoflorin (2) from their NMR spectroscopic and MS data. The identification of rutin in calyces of P. peruviana supports the possible use of this waste material for phytotherapeutic, nutraceutical and cosmetic preparations.

Anti-inflammatory activity of leaf extract and fractions of Bursera simaruba (L.) Sarg (Burseraceae)

2004 ◽  
Vol 92 (1) ◽  
pp. 129-133 ◽  
Author(s):  
B. Noguera ◽  
E. Dı́az ◽  
M.V. Garcı́a ◽  
A.San Feliciano ◽  
J.L. López-Perez ◽  
...  
Keyword(s):  
Leaf Extract ◽  
Inflammatory Activity ◽  
Bursera Simaruba ◽  
Anti Inflammatory

scholarly journals THE EFFECT OF MAHKOTA DEWA (PHALERIA MACROCARPA) (SCHEFF.) FRUIT PERICARP EXTRACT ON INOS IN MICE COLON INTERMITTENTLY-INDUCED BY DEXTRAN SODIUM SULFATE

2017 ◽  
Vol 10 (12) ◽  
pp. 309
Author(s):  
Kusmardi Kusmardi ◽  
Dilla Shavera ◽  
Ari Estuningtyas ◽  
Aryo Tedjo ◽  
Bambang Priyosoeryanto
Keyword(s):  
Sodium Sulfate ◽  
Total Yield ◽  
Ethanolic Extract ◽  
Dextran Sodium Sulfate ◽  
Inflammatory Activity ◽  
Fruit Pericarp ◽  
Phaleria Macrocarpa ◽  
Anti Inflammatory ◽  
Oxide Synthase ◽  
Different Doses

  Objective: The objective of this research was to investigate the anti-inflammatory effect of Mahkota Dewa fruit pericarp extract (Phaleria macrocarpa) on inducible nitric oxide synthase (iNOS) in mice colon induced by dextran sodium sulfate (DSS).Method: The simplisia of P. macrocarpa pericarp was weighed (1000 g) and extracted by maceration process. The total yield of the ethanolic extract was 26.43%. Phytochemical screening was carried out for the detection of the phytoconstituents by simple qualitative methods. The anti-inflammatory activity was performed by DSS-induced colitis model through assessment of hematoxylin-eosin staining and expression of iNOS by immunohistochemistry assay at four different doses, i.e., 650, 1250, 2500, and 5000 mg/kg. Swiss Webster male mice weighing 25-30 g were used for the study.Results: Inflammation score in dose 625, 1250, 2500, and 5000 mg/kg were 1.63, 1.43, 1.32, and 2.20, respectively. This result is significantly different (p=0.008) with DSS group that was 4.37. The results of iNOS optical density score in dose 625, 1250, 2500, and 5000 mg/kg were 1.21, 1.119, 1.22, and 1.37, respectively. This result was significantly different (p=0.000) with DSS group that was 2.24.Conclusion: Pericarp extract of P. macrocarpa fruit exhibited anti-inflammatory activity in the experimental model shown by suppressing the expression of inflammatory cell and iNOS. 

scholarly journals Anti-Inflammatory Activity of Populus deltoides Leaf Extract via Modulating NF-κB and p38/JNK Pathways

2018 ◽  
Vol 19 (12) ◽  
pp. 3746 ◽  
Author(s):  
Ye Jeong ◽  
Mi-Young Lee
Keyword(s):  
Nitric Oxide ◽  
Protein Kinase ◽  
Leaf Extract ◽  
Populus Deltoides ◽  
Nuclear Translocation ◽  
Mapk Signaling ◽  
Mitogen Activated Protein Kinase ◽  
Inflammatory Activity ◽  
No Production ◽  
Anti Inflammatory

Populus deltoides, known as eastern cottonwood, has been commonly used as a medicinal plant. The aim of the present study was to investigate the mechanism underlying the anti-inflammatory activity of P. deltoides leaf extract (PLE). PLE effectively inhibited the expression of inducible nitric oxide synthase (iNOS) and nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells, but not that of cyclooxygenase-2 (COX-2) and prostaglandin E2. Proinflammatory tumor necrosis factor alpha (TNF-α) levels were also reduced by the extract. PLE inhibited the phosphorylation of nuclear factor-kappa B (NF-κB) and inhibitor of Kappa Bα (IκBα), and blunted LPS-triggered enhanced nuclear translocation of NF-κB p65. In mitogen-activated protein kinase (MAPK) signaling, PLE effectively decreased the phosphorylation of p38 and c-Jun N-terminal protein kinase (JNK), but not of extracellular signal-regulated kinase 1/2 (ERK1/2). Taken together, these results suggest that anti-inflammatory activity of P. deltoides leaf extract might be driven by iNOS and NO inhibition mediated by modulation of the NF-κB and p38/JNK signaling pathways.

scholarly journals Analgesic and Anti-Inflammatory Activity of Total Crude Leaf Extract of <i>Phytolacca dodecandra</i> in Wistar Albino Rats

2019 ◽  
Vol 10 (03) ◽  
pp. 259-271
Author(s):  
Loyce Nakalembe ◽  
Josephine N. Kasolo ◽  
Edward Nyatia ◽  
Aloysius Lubega ◽  
Godfrey S. Bbosa
Keyword(s):  
Leaf Extract ◽  
Inflammatory Activity ◽  
Albino Rats ◽  
Phytolacca Dodecandra ◽  
Crude Leaf Extract ◽  
Anti Inflammatory ◽  
Wistar Albino Rats
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