scholarly journals Understanding microbial infections using microarray technology

2021 ◽  
Vol 2 (1) ◽  
pp. 131-135
Author(s):  
Lawrence O. Flowers
Keyword(s):  
Protein Interactions ◽  
Life Cycles ◽  
Infection Process ◽  
The Body ◽  
Survival Strategies ◽  
Body Region ◽  
Detailed Knowledge ◽  
Microarray Technology ◽  
Microbial Infection ◽  
Microbial Infections

Human microbial infections are symbiotic processes between pathogens and humans that often lead to human disease and death. Microbial infections involve the attachment, growth, and survival of microorganisms on human skin, inside the body, or inside specific cells. Microbial infections can be localized to one body region or migrate to secondary body locations utilizing various transport mechanisms. Understanding host-pathogen interactions related to the expression of essential genes during and after infection can lead to valuable information for biologists and clinicians. Microarray technologies allow researchers to perform genomic characterization experiments rapidly and efficiently. Microarray experiments support the resolution of underlying molecular events that play a role in normal and aberrant physiologic activities in living systems. Microarray technology, coupled with bioinformatics analysis, generates comprehensive insights into relevant genes, proteins, and protein-protein interactions. This review article explores recent microarray research studies from select protozoan and bacterial pathogens to illustrate how researchers utilize microarray technology to examine microbial infection aspects. Microarray studies of pathogen and host genomes at various stages of the infection process will generate a more precise understanding of pathogenic life cycles and pathogen survival strategies. Detailed knowledge of the genes involved in the microbial infection process will lead to discovering disease biomarkers and potent therapeutic solutions.

Life cycles of the rhizocephalan Boschmaella japonica Deichmann & Høeg, 1990 (Cirripedia: Chthamalophilidae) and its host barnacle Chthamalus challengeri Hoek, 1883 (Cirripedia: Chthamalidae)

2020 ◽  
Vol 40 (6) ◽  
pp. 825-832 ◽  
Author(s):  
Miku Yabuta ◽  
Jens T Høeg ◽  
Shigeyuki Yamato ◽  
Yoichi Yusa
Keyword(s):  
Life Cycle ◽  
Brood Size ◽  
Life Cycles ◽  
The Body ◽  
Egg Volume ◽  
Size Number ◽  
Rhizocephalan Barnacles ◽  
Western Japan ◽  
Upper Intertidal ◽  
Chthamalus Challengeri

Abstract Although parasitic castration is widespread among rhizocephalan barnacles, Boschmaella japonica Deichmann & Høeg, 1990 does not completely sterilise the host barnacle Chthamalus challengeri Hoek, 1883. As little information is available on the relationships with the host in “barnacle-infesting parasitic barnacles” (family Chthamalophilidae), we studied the life cycles of both B. japonica and C. challengeri and the effects of the parasite on the host reproduction. Specimens of C. challengeri were collected from an upper intertidal shore at Shirahama, Wakayama, western Japan from April 2017 to September 2018 at 1–3 mo intervals. We recorded the body size, number of eggs, egg volume, and the presence of the parasite for each host. Moreover, settlement and growth of C. challengeri were followed in two fixed quadrats. Chthamalus challengeri brooded from February to June. The prevalence of B. japonica was high (often exceeded 10%) from April to July, and was rarely observed from September to next spring. The life cycle of the parasite matched well with that of the host. The parasite reduced the host’s brooding rate and brood size, to the extent that no hosts brooded in 2018.

scholarly journals ORTHOPEDIC INJURIES IN ADOLESCENT GYMNASTS: A SYSTEMATIC REVIEW

2021 ◽  
Vol 9 (7_suppl3) ◽  
pp. 2325967121S0011
Author(s):  
Katie Kim ◽  
Michael Saper
Keyword(s):  
Lower Extremity ◽  
Upper Extremity ◽  
Weight Bearing ◽  
The Body ◽  
Adolescent And Young Adult ◽  
Body Region ◽  
Lower Extremity Injuries ◽  
Common Location ◽  
Orthopedic Injuries ◽  
Extremity Injuries

Background: Gymnastics exposes the body to many different types of stressors ranging from repetitive motion, high impact loading, extreme weight bearing, and hyperextension. These stressors predispose the spine and upper and lower extremities to injury. In fact, among female sports, gymnastics has the highest rate of injury each year. Purpose: The purpose of this study was to systematically review the literature on location and types of orthopedic injuries in adolescent (≤20 years) gymnasts. Methods: The Pubmed, Medline, EMBASE, EBSCO (CINAHL) and Web of Science databases were systematically searched according to the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) guidelines to identify all studies reporting orthopedic injuries in adolescent and young adult gymnasts. All aspects of injuries were extracted and analyzed including location, type and rates of orthopedic injuries. Results: Screening yielded 22 eligible studies with a total of 427,225 patients. Twenty of 22 studies reported upper extremity injuries of which four specifically focused on wrist injuries. Eight studies reported lower extremity injuries. Nine studies reported back/spinal injuries. Seven studies investigated each body location of injury; one study reported the upper extremity as the most common location for injury and six studies reported the lower extremity as the most common location for injury. Of those seven studies, five (23%) reported sprains and strains as the most common injury. One study reported fractures as the most common injury. Conclusion: There is considerable variation in reported injury location. Some studies focused specifically on the spine/back or wrist. The type of gymnastics each patient participated in was also different, contributing to which area of the body was more heavily stressed, or lacking. Current literature lacks data to fully provide evidence regarding which body region is more frequently injured and the type of injury sustained.

The Oxygen Consumption of an Echiuroid, Bonellia Viridis Rolando

1968 ◽  
Vol 48 (2) ◽  
pp. 427-434
Author(s):  
A. E. BRAFIELD
Keyword(s):  
Oxygen Consumption ◽  
Body Size ◽  
Continuous Flow ◽  
Muscular Activity ◽  
Dry Weight ◽  
The Body ◽  
Body Region ◽  
Rate Of Oxygen Consumption

1. The oxygen consumption of the echiuroid Bonellia viridis has been investigated by means of a continuous-flow polarographic respirometer. 2. The general rate of oxygen consumption per unit dry weight is similar to that characteristic of polychaetes, and declines exponentially with increasing body size. 3. The rate of oxygen consumption rises in the light and falls again if darkness is restored. 4. The oxygen consumption of the isolated proboscis plus that of the isolated body region corresponds closely to that of the entire animal. 5. The oxygen consumption per unit dry weight of the proboscis is considerably higher than that of the body region. 6. The oxygen consumption of an isolated body region increases in the presence of light, but that of an isolated proboscis does not. 7. These findings are discussed in relation to the biology of the animal, observed muscular activity, and the occurrence of the pigment bonellin.

THU0062 FUNCTIONAL MR IMAGING OF HUMAN MENISCUS IS ASSOCIATED WITH HISTOLOGICAL DEGENERATION

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 243-243
Author(s):  
P. Sewerin ◽  
L. Dötsch ◽  
D. Truhm ◽  
D. Abrar ◽  
S. Nebelung
Keyword(s):  
Mr Imaging ◽  
T2 Mapping ◽  
Lateral Meniscus ◽  
Compressive Force ◽  
The Body ◽  
Quantitative Mri ◽  
Imaging Biomarker ◽  
Body Region ◽  
Deutschland Gmbh ◽  
Loading Device

Background:In OA, there is a close association of meniscus and cartilage pathologies. Meniscus degeneration and lesioning are critical risk factors for development of early OA. Hence, thisex-vivostudy assessed the responses to standardized loading of human meniscus samples as a function of degeneration and based on changes in their T1, T2 and T1ρ maps (as surrogate parameters of the tissue’s functionality).Objectives:Can meniscus functionality be visualized by serial quantitative MRI mapping technics?Methods:During total knee replacements, 45 meniscus samples of variable degeneration were harvested from the center of the lateral meniscus body (Fig. 1a1-a3). After preparation to standard, samples were subject to force-controlled loading using an MRI-compatible lever device that created compressive loading by torque ((Fig. 1a4-a5). For each sample and loading position, MRI measurements (as detailed below) were performed in the unloaded (δ0) and loaded configurations, i.e. loaded to 2 bar (δ1, 37.1 N compressive force, 0.67 Nm torque) and to 4 bar (δ2, 69.1 N, 1.24 Nm). Throughout all loading positions, morphological and quantitative imaging was performed using Proton Density-weighted and T1, T1ρ, and T2 mapping sequences (3.0 T, Achieva, Philips) based on standard turbospin-echo, inversion-recovery, spin-lock multi-gradient-echo, and multi-spin-echo sequences. For reference purposes, histological (i.e. Pauli classification) and biomechanical measures (i.e. Elastic Modulus) were obtained for each sample. Based on Pauli sum scores, samples were trichotomized as grossly intact, (n=14), mildly degenerated (n=16), and moderate-to-severely degenerated (n=15).Figure 1.Preparation of meniscus samples and details of the MRI-compatible loading device. The lateral meniscus (a1) was cut to standard size by use of a dedicated cutting block (a2) to eventually obtain lateral meniscus samples (from the body region) of standard dimensions (a3). These samples were then placed in a dedicated MRI-compatible loading device for pressure-controlled, quasi-static and torque-induced loading under simultaneous MR imaging (a4). Two parallel support beams allowed standardized positioning in the MRI scanner‘s bore (a5).Results:Morphologically, loading induced deformation and flattening in all samples (Fig. 2a). For T1, homogeneous loading-induced decreases in all samples were found, irrespective of degeneration (Fig. 2b). For T1ρ, increases in the apical zones of intact samples were observed, and decreases in degenerated samples (Fig. 2c). For T2, changes were ambiguous and incoherent (Fig. 2d).Figure 2.Serial morphological images and functional maps of histologically moderately degenerative human meniscus as a function of force-controlled loading. Serial PDw (a), T1 (b), T1ρ (c), and T2 maps (d) are displayed at increasing loading intensity (δ0: unloaded [a1-d1]; δ1: loaded to 2 bar [a2-d2]; δ2: loaded to 4 bar [a3-d3]). Histologically, this sample demonstrated signs of severe surface desintegration and disruption. Pauli sum score 12, i.e. moderate to severe degeneration (Pauli Grade III). In b – d, color-coded parameter value maps are overlaid onto the corresponding morphological images. Histological sections are stained with Hematoxylin-Eosin (e1) and Safranin O (e2).Conclusion:Meniscus functionality may be visualized using serial quantitative MRI mapping techniques. T1ρ may provide an imaging biomarker of relevant intra-tissue adaptations that seem to be associated with histological degeneration. The perspective evaluation of meniscus functionality may be indicative of incipient or manifest load transmission failure to the adjacent cartilage layer.Disclosure of Interests:Philipp Sewerin Grant/research support from: AbbVie Deutschland GmbH & Co. KGBristol-Myers Squibb Celgene GmbHLilly Deutschland GmbHNovartis Pharma GmbH Pfizer Deutschland GmbHRheumazentrum Rhein-Ruhr, Consultant of: AMGEN GmbH AbbVie Deutschland GmbH & Co. KG Biogen GmbHBristol-Myers Squibb Celgene GmbH Chugai Pharma arketing Ltd. / Chugai Europe GmbHHexal Pharma Janssen-CilagGmbH Johnson & Johnson Deutschland GmbHLilly Deutschland GmbH / Lilly Europe / Lilly Global Novartis Pharma GmbH Pfizer Deutschland GmbH Roche Pharma Rheumazentrum Rhein-Ruhr Sanofi-Genzyme Deutschland GmbH Swedish Orphan Biovitrum GmbH UCB Pharma GmbH, Speakers bureau: AMGEN GmbH AbbVie Deutschland GmbH & Co. KG Biogen GmbHBristol-Myers Squibb Celgene GmbH Chugai Pharma arketing Ltd. / Chugai Europe GmbHHexal Pharma Janssen-CilagGmbH Johnson & Johnson Deutschland GmbHLilly Deutschland GmbH / Lilly Europe / Lilly Global Novartis Pharma GmbH Pfizer Deutschland GmbH Roche Pharma Rheumazentrum Rhein-Ruhr Sanofi-Genzyme Deutschland GmbH Swedish Orphan Biovitrum GmbH UCB Pharma GmbH, Lisa Dötsch: None declared, Daniel Truhm: None declared, Daniel Abrar: None declared, Sven Nebelung: None declared

A Cross-Sectional Study on Prevalence of Musculoskeletal Pain in Dhol Players in India

2021 ◽  
Vol 36 (2) ◽  
pp. 72-77
Author(s):  
Prachita Walankar ◽  
Ninisha Patil
Keyword(s):  
Musculoskeletal Pain ◽  
Specific Area ◽  
Cross Sectional Study ◽  
The Body ◽  
Body Region ◽  
Targeted Prevention ◽  
Low Back ◽  
Sectional Study ◽  
Common Site ◽  
Cross Sectional

OBJECTIVE: To explore the prevalence of musculoskeletal pain in dhol players in India. METHODS: A cross-sectional study was conducted in 96 dhol players. A self-administered questionnaire comprising of demographic profile, dhol player characteristics, presence of musculoskeletal pain, and specific area of pain according to the body region was used to collect information from the dhol players. RESULTS: The mean age and duration of playing dhol experience were 22.26+3.05 and 6.08+1.27 years, respectively. Among 96 dhol players, 93 (96.9%) and 55 (57.3%) reported presence of musculoskeletal pain with respect to the 12-month prevalence and 7-day prevalence, respectively. In 12-month prevalence, the most common site of pain was the low back (67.7%), followed by shoulder (54.2%) and wrist and hand (24%). In 7-day prevalence, the most common site of pain was the low back (41.7%), followed by shoulder (24%) and upper back (15.6%). Logistic regression analysis revealed that age (p=0.002) and duration of playing dhol (p<0.001) were associated with 7-day prevalence of musculoskeletal pain. CONCLUSION: The findings of this study indicated high prevalence of musculoskeletal pain in dhol players. Low back, shoulder, wrist/hand, and upper back were common sites of pain. Identification of these factors in dhol players may assist in further research on targeted prevention, scientific recommendations, and rehabilitation.

scholarly journals Studies of the Nucleopolyhedrovirus Infection Process in Insects by Using the Green Fluorescence Protein as a Reporter

1998 ◽  
Vol 72 (4) ◽  
pp. 3377-3382 ◽  
Author(s):  
John W. Barrett ◽  
Andy J. Brownwright ◽  
Mark J. Primavera ◽  
Subba Reddy Palli
Keyword(s):  
Fat Body ◽  
Secondary Infection ◽  
Green Fluorescence Protein ◽  
Body Cavity ◽  
Infection Process ◽  
The Body ◽  
Green Fluorescence ◽  
Temporal Regulation ◽  
Baculovirus Infection ◽  
Fluorescence Protein

ABSTRACT A recombinant Autographa californica multicapsid nucleopolyhedrovirus (AcMNPV) expressing the green fluorescence protein (GFP) under the control of the AcMNPV polyhedrin promoter was constructed to study the spatial and temporal regulation of baculovirus infection in a permissive host. Larvae that ingested AcMNPV-GFP showed localized expression of GFP in the midgut epithelial cells, as well as hemocytes, at 24 h postinfection. The presence of fluorescence in these tissues indicated not only that the virus was replicating but also that the very late viral proteins were being synthesized. Secondary infection occurred within the tracheal cells throughout the body cavity, confirming earlier reports, and these foci of infection allowed entry of the virus into other tissues, such as the epidermis and the fat body.

scholarly journals Discovering the forbidden Raman modes at the edges of layered materials

2018 ◽  
Vol 4 (12) ◽  
pp. eaau6252 ◽  
Author(s):  
Yao Guo ◽  
Weixuan Zhang ◽  
Hanchun Wu ◽  
Junfeng Han ◽  
Yongliang Zhang ◽  
...  
Keyword(s):  
Raman Spectra ◽  
Electromagnetic Fields ◽  
Scattered Light ◽  
Layered Materials ◽  
The Body ◽  
Body Region ◽  
Edge Type ◽  
Body Regions ◽  
Raman Modes ◽  
Raman Study

The edges of layered materials have unique properties that substantially differ from the body regions. In this work, we perform a systematic Raman study of the edges of various layered materials (MoS2, WS2, WSe2, PtS2, and black phosphorus). The Raman spectra of the edges feature newly observed forbidden Raman modes, which are originally undetectable from the body region. By selecting the edge type and the polarization directions of the incident and scattered light, all forbidden Raman modes are distinctly detected. Optical simulations show that the edges of layered materials drastically distort the electromagnetic fields of both the incident and scattered light, so that the light interacts with the edges in a distinct way, which differs from its interactions with the body regions.

scholarly journals Assessing the risk for major injuries in equestrian sports

2018 ◽  
Vol 4 (1) ◽  
pp. e000408 ◽  
Author(s):  
Lara Krüger ◽  
Maike Hohberg ◽  
Wolfgang Lehmann ◽  
Klaus Dresing
Keyword(s):  
Surgical Therapy ◽  
Lower Limb ◽  
Head Injuries ◽  
Trauma Centre ◽  
The Body ◽  
Body Region ◽  
Older Individuals ◽  
Horse Riding ◽  
Limb Injuries ◽  
Prevention Programmes

Background/aimHorse riding is a popular sport, which bears the risk of serious injuries. This study aims to assess whether individual factors influence the risk to sustain major injuries.MethodsRetrospective data were collected from all equine-related accidents at a German Level I Trauma Centre between 2004 and 2014. Logistic regression was used to identify the risk factors for major injures.Results770 patients were included (87.9% females). Falling off the horse (67.7%) and being kicked by the horse (16.5%) were the two main injury mechanisms. Men and individuals of higher age showed higher odds for all tested parameters of serious injury. Patients falling off a horse had higher odds for being treated as inpatients, whereas patients who were kicked had higher odds for a surgical therapy (OR 1.7) and intensive care unit/intermediate care unit (ICU/IMC) treatment (OR 1.2). The head was the body region most often injured (32.6%) and operated (32.9%). Patients with head injuries had the highest odds for being hospitalised (OR 6.13). Head or trunk injuries lead to the highest odds for an ICU/IMC treatment (head: OR 4.37; trunk: OR 2.47). Upper and lower limb injuries showed the highest odds for a surgical therapy (upper limb: OR 2.61; lower limb: OR 1.7).ConclusionRisk prevention programmes should include older individuals and males as target groups. Thus a rethinking of the overall risk assessment is necessary. Not only horseback riding itself, but also handling a horse bears a relevant risk for major injuries. Serious head injures remain frequent, serious and an important issue to be handled in equestrians sports.

scholarly journals Change of Intestinal Microbiome in Patients with Coronavirus Infection

2021 ◽  
Vol 6 (5) ◽  
pp. 22-27
Author(s):  
M. M. Mishina ◽  
◽  
O. V. Kotsar ◽  
Pochernina M. H. ◽  
O. V. Kochnieva ◽  
...  
Keyword(s):  
Bacterial Translocation ◽  
Intestinal Barrier ◽  
Multiple Organ ◽  
Infection Process ◽  
The Body ◽  
Intestinal Microbiome ◽  
Intestinal Lumen ◽  
Treatment Regimens ◽  
Analysis And Synthesis ◽  
Coronavirus Infection

The purpose of the study was to analyze modern literature on the problems of dysbiosis in patients with COVID-19, to study the main mechanisms of systemic interaction between the intestine and lungs, as well as changes in the microbiota that occur under the influence of coronavirus infection. Materials and methods. A comprehensive selection of research methods was used for the work: systematization of the material, the method of generalization, methods of analysis and synthesis. Scientific works in the field of microbiology, epidemiology and infectious diseases were studied. Literature data for the last 2 years (2019-2021) were considered. The results of bacteriological studies from patients with COVID infection were described. The data obtained were processed using information-analytical and statistical-analytical methods. Results and discussion. As a result of this work, a complex of connections between intestine and lungs, which is called the "intestinal-lung axis", was considered. It is known that the interaction between these two biotopes occurs with the participation of microflora and its metabolites. Dysfunction of the intestinal barrier is accompanied by bacterial translocation. Bacteria from the intestinal lumen enter the liver through the portal vein system. The lymphatic pathway of bacterial translocation from the intestine to the lungs is also possible, which causes multiple organ failure syndrome in coronavirus infection. The COVID-19 virus is able to reduce the number of ACE2 receptors in the gastrointestinal tract, which leads to an imbalance in the intestines. At the same time, the infection process in the lungs promotes the growth of bacteria of the Enterobacteriacae family in the intestine, which also leads to dysbiotic disorders. The use of probiotics is an effective tool in the complex treatment of this infection, which facilitates the general condition of patients. In the course of treatment, it is important not only to eliminate the virus from the body, but also to restore normal intestinal microbiota after an infection. Conclusion. Thus, the use of probiotic drugs for the treatment of patients with coronavirus infection can significantly reduce the risk of developing dysbiosis and improve the condition of patients. A perspective direction is the development of new treatment regimens for dysbiotic conditions using probiotics, eubiotics, synbiotics and postbiotics to prevent the development of severe complications in COVID infection

scholarly journals Scanning electron microscopic observation of the infection process of a Metarhizium strain Ma6 highly pathogenic to Phyllotreta striolata

2018 ◽  
Author(s):  
Jianyu Li ◽  
Mengzhu Shi ◽  
Yuechao He ◽  
Jianwei Fu ◽  
Lizhen Zheng
Keyword(s):  
Body Surface ◽  
Electron Microscopic Observation ◽  
Infection Process ◽  
The Body ◽  
Body Parts ◽  
Pathogenic Microorganisms ◽  
Electron Microscopic ◽  
Highly Pathogenic ◽  
Phyllotreta Striolata ◽  
Scanning Electron

Background. Phyllotreta striolata is a worldwide pest that harms cruciferous vegetables. The use of pathogenic microorganisms to control pests is an important means of biological control. Using pathogenic microorganisms to prevent and control P. striolata has rarely been reported. Methods. In this study, the infection process of a Metarhizium strain highly pathogenic to P. striolata was observed by stereomicroscopyand scanning electron microscopy (SEM). Results.The results showed that the attachment of Metarhizium strain Ma6 to the body surface varied; the conidia distribution was greatest in the tibia of the posterior leg with thick bristles and in the intersegmental abdominal membrane, and the spore distribution occurred least in the smooth and hard portions of the insect’s body. At the start of the infection, Metarhizium strain Ma6 generally grew from the body parts with gaps or connecting spaces such as mouthparts and the thoracic leg base and joints, then the spores germinated with germ tubes and penetration peg, and the penetration peg penetrated the body surface. Ten days after inoculation, the mycelia divided into conidia, and many mycelia and spores covered the entire adult insect’s body. Discussion. Spore germination occurred on the 5th day after inoculation, and many hyphae and spores covered the entire adult insect body within 10 days after inoculation. And the invasion into tissue gaps from the weaker areas is more efficient than intruding from the body hard surface. This may be the reason for the Metarhizium strain Ma6’s high virulence. This study preliminarily clarified the infection ability and invasion approach of a Metarhizium strain against P. striolata, providing evidence for evaluating the strain’s insecticidal effect and application prospect.

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