Assessment of Serum Cystatin C Levels in Women with Breast Cancer Compared to the Control Group

Background: A good survival rate among patients with beta thalassemia major (beta-TM) has led to the appearance of an unrecognized renal disease. Therefore, we aimed to assess the role of serum cystatin-C as a promising marker for the detection of renal glomerular dysfunction and N-acetyl beta-D-glucosaminidase (NAG) and kidney injury molecule 1 (KIM-1) as potential markers for the detection of renal tubular injury in beta-TM children. Methods: This case-control study was implemented on 100 beta-TM children receiving regular blood transfusions and undergoing iron chelation therapy and 100 healthy children as a control group. Detailed histories of complete physical and clinical examinations were recorded. All subjected children underwent blood and urinary investigations. Results: There was a significant increase in serum cystatin-C (p < 0.001) and a significant decrease in eGFR in patients with beta-TM compared with controls (p = 0.01). There was a significant increase in urinary NAG, KIM-1, UNAG/Cr, and UKIM-1/Cr (p < 0.001) among thalassemic children, with a significant positive correlation between serum cystatin-C, NAG and KIM-1 as regards serum ferritin, creatinine, and urea among thalassemic patients. A negative correlation between serum cystatin-C and urinary markers with eGFR was noted. Conclusion: Serum cystatin-C is a good marker for detection of glomerular dysfunction. NAG and KIM-1 may have a predictive role in the detection of kidney injury in beta-TM children.

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Activity of Proteolytic Enzymes and Level of Cystatin C in the Peripartum Period

BioMed Research International ◽

10.1155/2016/7065821 ◽

2016 ◽

Vol 2016 ◽

pp. 1-5

Author(s):

Anna Cyganek ◽

Aleksandra Wyczalkowska-Tomasik ◽

Patrycja Jarmuzek ◽

Barbara Grzechocinska ◽

Zoulikha Jabiry-Zieniewicz ◽

...

Keyword(s):

Cystatin C ◽

Cathepsin B ◽

Proteolytic Enzymes ◽

Late Pregnancy ◽

Control Group ◽

Study Group ◽

Serum Cystatin C ◽

Serum Cystatin ◽

Peripartum Period

Objectives. The aim of the study was to evaluate the activity of cathepsin B, collagenases, trypsin, and plasmin and concentration of cystatin C in serum of healthy pregnant women in peripartum period.Study Design. The study group included 45 women in uncomplicated pregnancies. Blood samples were collected in four time points. Enzyme activity was measured by spectrofluorometric method. The level of cystatin C was measured using immunonephelometric method.Results. Mean activity of cathepsin B and the level of serum cystatin C were significantly higher in the study group. Collagenase activity was significantly lower in the study group than the control group. No differences in collagenase, plasmin, and trypsin activity on each day of the peripartum period were found.Conclusion. High activity of cathepsin B and increased level of cystatin C are typical for women in late pregnancy. Those levels significantly decrease after delivery which can be associated with potential role of those markers in placental separation. The insignificant changes of cystatin C level in the peripartum period seem to exclude the possibility of using cystatin C as a marker for renal insufficiency in the peripartum period but additional research is necessary to investigate the matter further.

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Cystatin C-Based Evaluation of Kidney Function of HIV-Infected Children in Benin City, Southern Nigeria

International Journal of Nephrology ◽

10.1155/2012/861296 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 2

Author(s):

Moses Temidayo Abiodun ◽

Nosakhare J. Iduoriyekemwen ◽

Phillip O. Abiodun

Keyword(s):

Kidney Disease ◽

Hiv Infection ◽

Kidney Function ◽

Cystatin C ◽

Mean Value ◽

Control Group ◽

Healthy Children ◽

Serum Cystatin C ◽

Serum Cystatin ◽

The Mean

Background. Human immunodeficiency virus (HIV) is now a confirmed risk factor for kidney disease with an increased burden in persons of African descent.Method. We measured the serum cystatin C levels of 205 ART-naive, HIV-infected children by an ELISA technique and compared them with the levels of apparently healthy children.Result. The mean ± SD serum cystatin C level of children with HIV infection was 1.01 ± 0.44 mg/L, significantly higher than the mean value in the control group, that is, 0.72 ± 0.20 mg/L (P=0.000). The mean ± SD cystatin C-based estimated GFR of children with HIV infection was 102.7 ± 31.0 mL/min/1.73 m2, significantly lower than 126.9 ± 28.5 mL/min/1.73 m2in the control group, (P=0.014). A significantly higher proportion of HIV-infected children compared to controls had eGFR < 90 mL/min/1.73 m2(21.5% versus 5.4%;P=0.00). The prevalence of chronic kidney disease (CKD) among the HIV-infected children was 10.7%. The cystatin C-based eGFR of the HIV-infected children ≥5 years old correlated positively with their CD4 count (r=0.23; P=0.022).Conclusion. There is a high prevalence of CKD among HIV-infected children, requiring regular monitoring of their kidney function using a cystatin C-based method.

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PENGARUH PEMBERIAN KONTRAS MEDIA NON IONIK LOW OSMOLAR INTRA VENA TERHADAP KADAR CYSTATIN–C SERUM PADA ORYCTALAGUS CUNICULUS

Indonesian Journal of Urology ◽

10.32421/juri.v15i2.354 ◽

1970 ◽

Vol 15 (2) ◽

Author(s):

Ariyo Sakso Bintoro ◽

Soetojo Soetojo ◽

Doddy M Soebadi

Keyword(s):

Cystatin C ◽

Sandwich Elisa ◽

Intervention Group ◽

Serum Levels ◽

Control Group ◽

Test Results ◽

Elisa Method ◽

Serum Cystatin C ◽

Serum Cystatin ◽

Ionic Contrast

Objective: To measure effects of low osmolar non ionic contrast media on cystatin–C serum levels on different days after administration. Material and Method: Twenty-two oryctalagus cuniculus were divided into two groups of 11 subjects, each subject receiving intravenous injection of low osmolar non ionic contrast or a placebo solution (NaCl 0,9%). Cystatin-C serum levels of each subject were measured before injection, on days 1, 3, 7, and 10. Cystatin–C serum levels were determined with a sandwich ELISA method. Statistical analysis was performed with t–test. Results: Mean cystatin–C serum levels before injection was 0,00337 ± 0,00101 mg/L. Means from days 1, 3, 7, and 10 after injections were 0,00498 ± 0,00153 mg/L; 0,00565 ± 0,00247 mg/L; 0,00468 ± 0,00157, and 0,00339 ± 0,00188 mg/L respectively. Conclusion: Increase in serum cystatin–C levels on days 1, 3, and 7 was significant. On the 10th day no significant escalation was observed. Compared to the control group, there were significant differences in serum cystatin–C increase on days 1 and 3 in the intervention group, but no significant differences 7 and 10 days after injection.

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Serum Cystatin C in Estimating Glomerular Filtration Rate

Journal of Medical Biochemistry ◽

10.2478/v10011-007-0042-4 ◽

2008 ◽

Vol 27 (1) ◽

pp. 46-51 ◽

Cited By ~ 1

Author(s):

Velibor Čabarkapa ◽

Zoran Stošić ◽

Mirjana Đerić ◽

Ljiljana Vučurević-Ristić ◽

Radmila Žeravica ◽

...

Keyword(s):

Glomerular Filtration Rate ◽

Renal Insufficiency ◽

Glomerular Filtration ◽

Cystatin C ◽

Chronic Renal Insufficiency ◽

Filtration Rate ◽

Control Group ◽

Serum Cystatin C ◽

Serum Cystatin ◽

Subgroup Ii

Serum Cystatin C in Estimating Glomerular Filtration RateUsing serum cystatin C in estimating glomerular filtration rate (GFR) has in recent times been recommended. A number of simple formulas for calculating GFR have been derived specifically from serum cystatin C concentrations. The purpose of this study was to assess the significance of cystatin C and of the two most frequently applied of these formulas in estimating glomerular filtration rate compared to serum creatinine and its derived formulas for estimating glomerular filtration rate from creatinine concentrations. The study included 74 patients: 59 were in various stages of chronic renal insufficiency (divided into two subgroups: I with GFR ≥ 60 mL/min/1.73m2and II with GFR<60 mL/min/1.73m2) and 15 on hemodialysis. A control group of 30 healthy participants was also included in the study. Serum values of cystatin C ranged from: 0.86 ± 0.16 mg/L in subgroup I, and 1.77 ± 0.79 mg/L in subgroup II, to 6.9 ± 1.83 mg/L in patients on hemodialysis. The correlation between the two formulas derived from cystatin C and the clearance of creatinine, as well as the Cockcroft and Gault's formula, was significant, while one of the formulas derived from cystatin C did not show a significant correlation with MDRD. It was concluded that serum cystatin C is a significant marker in estimating glomerular filtration rate, especially in the advanced stages of chronic renal insufficiency.

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A Comparative Study of Serum Cystatin C, Serum Electrolytes, Urea and Creatinine in Early Detection of Kidney Injuries in Albino Rats Exposed to Formaldehyde

10.54117/ijt.v1i1.1.2021 ◽

2021 ◽

Vol 1 (1) ◽

pp. 1-4

Author(s):

Michael Chinedu Olisah ◽

◽

Samuel C. Meludu ◽

Keyword(s):

Cystatin C ◽

Low Dose ◽

Control Group ◽

Albino Rats ◽

High Dose ◽

Serum Cystatin C ◽

Serum Electrolytes ◽

Serum Cystatin ◽

Group A ◽

Group B

Aim: To ascertain early detection of possible kidney injuries in albino rats exposed to formaldehyde by assessing cystatin C, serum electrolytes, urea and creatinine. Materials/Methods: Thirty healthy adult male albino rats, weighing between 100 to 120 grams were randomly divided into three groups A, B and C. Group A served as control. Group B was exposed to low dose (100ppm of Formaldehyde), 3 hrs per day for four weeks, while group C was exposed to high Dose-200ppm of formaldehyde 3 hrs per day for four weeks. At the end of the exposure period, the rats were sacrificed by decapitation under chloroform anesthesia, and 4 ml of blood samples was collected from each rat into a plain bottle. The whole blood was allowed to clot, retracted and centrifuged at 3000 rpm for 10 minutes and serum separated. The serum was stored at -20˚C until analyses for serum electrolytes, creatinine and cystatin C. Serum electrolytes were determined using Ion selective electrode, urea and creatinine were determined using spectrophotometric methods while cystatin C was analyzed using Eliza. Data obtained was analyzed using SPSS. Results: The concentrations of the serum electrolytes, sodium. Potassium, bicarbonate and chloride were compared across the three group, they were not statistically significant (p>0.05). Urea was significantly higher in group C when compared with low dose B and control group A (P<0.05). Also, when the low dose group B was compared with the high dose group C, it was statistically significant. Creatinine concentrations were significantly higher in group C when compared with the control group A, while group B was not significant when compared with group A. Finally, the cystatin C concentrations were also significant when groups B, C were compared with the control group. Conclusion: Formaldehyde exposures may induce a gradual deterioration of renal functions in chronically exposed albino rats. Serum electrolytes, urea and creatinine may not be sufficient to indicate an early signs of kidney damage. From the study, serum cystatin C may be a better marker of renal impairment in early stages.

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STUDY OF RENAL FUNCTION IN TYPE 2 DIABETIC PATIENTS BY SERUM CYSTATIN C

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2016.6.7 ◽

2017 ◽

pp. 44-51

Author(s):

Anh Dao Dang ◽

Huu Dang Tran

Keyword(s):

Glomerular Filtration Rate ◽

Cystatin C ◽

Filtration Rate ◽

Control Group ◽

Diabetic Patients ◽

Serum Cystatin C ◽

Type 2 Diabetic Patients ◽

Serum Cystatin ◽

Type 2 Diabetic

Aims: to investigate serum Cystatin C level, correlation between serum Cystatin C levels with albuminuria, estimate glomerular filtration rate (eGFR), and renal radiography in type 2 diabetic patients. Materials and methods: We studied 77 healthy subjects without prediabetes or diabetes, and 70 type 2 diabetic patients. We excluded patients with thyroid dysfunction and those taking glucocorticoids that affect the serum Cystatin level. The serum Cystatin C level measured in 2 groups. In type 2 diabetic patients, we measured renal radiography, albuminuria, and estimated GFR using CKD.EPI 2012 formular. Descriptive and cross-sectional study. A statistical analyses of the data was performed using SPSS, Excel 2003. Receiver operating characteristics (ROC) analysis, calculated the area under the curve (AUC) for serum Cystatin C. Results: The level of serum Cystatin C in the type 2 diabetic patients was significantly higher than in the control group (1.45±0.97, 0.97±0.23 mg/L, p<0.001, respectively). The level of serum Cystatin C showed stepwise increase with albuminuria level. The serum cystatin C level in the macroalbuminuria group was significantly higher than microalbuminuria group (2,51±1,13; 1,12±0.69 mg//l, p<0.05, respectively). A positively correlation was observed between eGFR according to CKD.EPI equation based on serum Cystatin C and renal radiography (r=0.710, p<0.001). The positively correlation between eGFR according to CKD.EPI equation based on serum Creatinine-Cystatin C and renal radiography r=0.746, p<0.001). The serum Cystatin C level was significantly higher in patients with GFR < 60 ml/min/1.73m2 than those with ≥ 60 ml/min/1.73m2 (p < 0,001). Serum Cystatin C level was significantly reciprocal correlation between eGFR based on cystatin C serum (r=0.830, p<0.001) or renal radiography (r=0,748, p<0,001). The cutoff value for the identification of GFR < 60ml/min/1.73m2 was 1.28mg/l with a sensitivity of 100.0 (95% CI: 85,6-100,0) and specificity of 95.7 (95%CI:85.1-99.3). AUC was 0,992. Conclusions: The serum cystatin C level in type 2 diabetic patients was significantly higher than the control group. The serum level of Cystatin C in type 2 diabetic patients was stepwise increased with albuminuria level. There was a positive correlation between eGFR based on Cystatin C and renal radiography. A reciprocal correlation between serum Cystatin C level and eGFR calculated by CKD.EPI 2012 formular and GFR by renal radiography. Key words: diabetes, glomerular filtration rate, Cystatin C, albuminuria

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Association of Serum Cystatin C with Pre-eclampsia: A Case Control Study

Bangladesh Journal of Obstetrics & Gynaecology ◽

10.3329/bjog.v32i1.56731 ◽

2017 ◽

Vol 32 (1) ◽

pp. 29-32

Author(s):

Nasima Akhter ◽

Farzana Deeba ◽

Begum Nasrin ◽

Saleha Begum Chowdhury

Keyword(s):

Cystatin C ◽

Case Control Study ◽

Case Control ◽

Control Group ◽

P Value ◽

Serum Cystatin C ◽

Serum Cystatin ◽

Control Serum ◽

The Mean ◽

Control Study

Background: Cystatin C, an endogenous marker of renal function, which increases in pregnancy as more so in pre-eclampsia, studies show it may be marker of pre-eclampsia. Objective (s): The objective of the present work was to investigate the association between serum cystatin C levels in normal and pre-eclamptic pregnancy. Methods and material: This case control study was conducted in the department of obstetrics and gynaecology in three tertiary care hospitals of Dhaka city (BSMMU, DMC, BIRDEM ) during the period from January 2009 to December 2010. A total 240 women of 20-40 wks of pregnancy were enrolled in the study. One hundred and twenty four women of pre-eclampsia were recruited as case and 116 healthy pregnant women were enrolled as control. Serum cystatin C was measured in both case and control by a fully automated particle-enhanced immune-turbidimetric assay for cystatin C in undiluted samples. Serum cystatin C level was compared between the groups. P value reached from unpaired student t-test and chi square test by analyzing the data. P value less than 0.05 was considered to be significant. Results: The mean (±SD) serum cystatin C levels in case group was 1.17±0.34 mg/lranged from 0.1-2.2 mg/l. In control groups the mean (±SD) serum cystatin C level was 0.87±0.31 mg/l, ranged from 0.24-1.98 mg/l, which was significantly higher (p<0.001) in case. There was statistically significant association between serum cystatin C and pre-eclampsia. Serum Cystatin C level >1mg/l was found in more than half (69%) of the cases compared to 25% of the control group (p<0.001). Computation of odds ratio reveals that the cases had 6.79 times higher risk of having pre-eclampsia than that of the control group (p<0.001). Conclusion: There is significant association between pre-eclampsia and serum cystatin C Bangladesh J Obstet Gynaecol, 2017; Vol. 32(1): 29-32

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PERBANDINGAN CYSTATIN C DENGAN PARAMETER UJI FUNGSI GINJAL LAINNYA

INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY ◽

10.24293/ijcpml.v14i1.919 ◽

2018 ◽

Vol 14 (1) ◽

pp. 16

Author(s):

Pusparini .

Keyword(s):

Renal Failure ◽

Chronic Renal Failure ◽

Serum Creatinine ◽

Creatinine Clearance ◽

Cystatin C ◽

Normal Serum ◽

Control Group ◽

Serum Cystatin C ◽

Serum Cystatin ◽

Failure Group

The Gold standard for the evaluation of the glomerular filtration rate (GFR) is inulin clearance, but in widespread use is prevented by several technical difficulties. The most commonly used marker for GFR is serum creatinine alone or in conjunction with 24 hoururine collection for determination of creatinine clearance, but these marker have several limitation include following: influence of age,sex, muscle mass on endogenous creatinine production, dietary intake and the difficulties of 24 hour urine collection. Fifty six patientwith chronic renal failure and 53 control had analyze for serum creatinin, creatinine clearance and serum cystatin C. The chronic renalfailure patient aged range from (64 + 14.54) year and the control group aged range from (62.5+ 17.5) year. The proposed of this studywas to compare cystatin C with another parameter for renal function test. The result showed that in control group serum creatinineand creatinine clearance had influence with age, sex and body mass index, but serum cystatin C was not. The normal value of cystatinC was (0.85 + 0.13) mg/dL In chronic renal failure group there were significant correlation between level of cystatin C with creatininclearance (p = 0.000, r = 0.69). The level of cystatin C increase higher than serum creatinine in patient with low clearance creatinine.In control group we were determined low creatinine clearance in patient with normal serum creatinine and cystatin C.

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509-P: Utility of Serum Cystatin C in Early Kidney Dysfunction in Prediabetics

Diabetes ◽

10.2337/db19-509-p ◽

2019 ◽

Vol 68 (Supplement 1) ◽

pp. 509-P

Author(s):

JULIA I.F. BRANDA ◽

BIANCA ALMEIDA ◽

SANDRA R.G. VIVOLO

Keyword(s):

Cystatin C ◽

Kidney Dysfunction ◽

Serum Cystatin C ◽

Serum Cystatin

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