scholarly journals Use of growth regulators and experimental LED phytoirradiator in clonal micropropagation of garden strawberry (Fragaria × ananassa, Duchesne ex Weston)

2019 ◽  
Vol 20 (4) ◽  
pp. 324-333 ◽  
Author(s):  
M. G. Markova ◽  
E. N. Somova
Keyword(s):  
Experimental Data ◽  
Growth Regulators ◽  
Growth Regulator ◽  
Fragaria Ananassa ◽  
Clonal Micropropagation ◽  
Fluorescent Lamps ◽  
Combined Use ◽  
The Impact ◽  
Reproduction Factor

The article provides experimental data of 2017-2018 study on the effect of growth regulators and LED phytoirradiator on the proliferation and rooting of promising garden strawberry (Fragaria ananassa) varieties in vitro. Micro-shoots of Korona and Brighton strawberry varieties were taken as the object of the research. Strawberry micro-shoots were cultivated under fluorescent lamps in the control variant. A programmable combined blinking LED phytoirradiator was under study. The combined effect of cytokinin and gibberellic acid by adding them to the Murashige and Skoog nutrient medium, as well as the impact of Siliplant and EcoFus growth regulators on strawberry micropropagation has been studied. It was established that in the cultivation of Korona variety the combined use of Siliplant and EcoFus under illumination with LED phytoirradiator provided an increase in the reproduction factor. The coefficient was 5.0 pcs./explant that was 1.7 times higher than the control (3.0 pcs/explant), the LSD05 1.4 pcs/explant. The maximum reproduction factor of remontant strawberry Brighton variety was obtained in the variant with the use of Siliplant and LED phytoirradiator and amounted to 4.9 pcs./explant (4.2 pcs./explant in the control), the LSD05 was 1.5 pcs./ explant. Regardless of the lighting, the use of RibavExtra in all variants under study increased the rooting rate of the strawberry Korona micro-shoots from 92.8 to 99.1%, the LSD05 6.1%. The use of LED phytoirradiator in comparison with the luminescent one (94.3%) provided a significant increase in the rooting rate of the strawberry Korona micro-shoots to 98.1% regardless of the growth regulators used, the LSD05 3.5%. The combined use of LED phytoirradiator and Ribav-Extra growth regulator in concentrations of 1.0 and 1.5 mg/l resulted in rooting of strawberry Korona micro-shoots up to 100%. Regardless of the growth regulator used, the use of LED phytoirradiator in comparison with the luminescent one (88.9%) provided a significant increase in the rooting rate of the strawberry Brighton micro-shoots to 97.2%, the LSD05 4.6%. The rooting rate of the remontant strawberry Brighton microshoots was 100% in the variant with the use of Ribav-Extra in the concentration of 1.0 mg /l combined with LED phytoirradiator 20 days after transplanting for rooting.

scholarly journals Effect of Light on Rhizogenesis of Forest Berry Plants during Clonal Micropropagation

2021 ◽  
pp. 520-528
Author(s):  
Sergey Makarov ◽  
Sergey Rodin ◽  
Irina Kuznetsova ◽  
Anton Chudetsky ◽  
Svetlana Tsaregradskaya
Keyword(s):  
In Vitro Rooting ◽  
Light Emitting ◽  
Clonal Micropropagation ◽  
Fluorescent Lamps ◽  
Planting Material ◽  
Medicinal Value ◽  
American Cranberry ◽  
Food Market ◽  
Spectral Ranges

Introduction. Forest berry plants are popular on the food market and in pharmacy for their high nutritional and medicinal value. Plantations of forest berry plants can proliferate on unused lands, including depleted peatlands. Clonal micropropagation is the most effective method for obtaining large quantities of high quality planting material. Light-emitting diodes are highly effective for clonal micropropagation. The research objective was to study the effect of different spectral ranges on the process of root formation of forest berry plants in vitro. Study objects and methods. The research featured regenerant plants of half-highbush blueberry, arctic bramble, American cranberry, European cranberry, lingonberry, and Kamchatka bilberry of different cultivars. A set of experiments made it possible to study the effect of lighting type on the growth and development of the root system of forest berry plants in vitro using white fluorescent lamps, white spectrum LED lamps, and LED lamps with a combination of white, red, and blue spectra at the in vitro rooting stage of clonal micropropagation. Results and its discussion. The largest number (3.4–14.6 pcs.) and the maximum total length (10.0–156.9 cm) of roots were observed under LED lamps with a combination of white, red, and blue spectra. The effect was by 1.1–2.8 and 2.0–4.5 times higher than in the case of white-spectrum LED lamps, and by 2.3–7.0 and 3.3–14.9 times than in the case of fluorescent lamps. Variety and shape proved to have no significant effect on biometric indicators. Conclusion. LED lamps had a positive effect on the process of rhizogenesis of forest berry plants during clonal micropropagation. They appeared to be more effective than fluorescent lamps. The combination of white, blue, and red spectra increased the biometric parameters of plants at the stage of in vitro rooting.

scholarly journals In Vitro Propagation of Lilium longiflorum Bulbs Using NAA and BAP Plant Growth Regulator Treatment

2020 ◽  
Author(s):  
Ni Wayan Deswiniyanti ◽  
Ni Kadek Dwipayani Lestari
Keyword(s):  
Growth Regulators ◽  
Growth Regulator ◽  
In Vitro Propagation ◽  
Culture Media ◽  
Lilium Longiflorum ◽  
Herbaceous Plant ◽  
Perennial Herbaceous Plant ◽  
Plant Parts ◽  
Lily Bulb

Lily (Lilium longiflorum) is a perennial herbaceous plant with white trumpet-shaped flowers, fragrant and bulbous. In vitro culture through bulbs is one of way propagation of lily plants, but it requires a long time and only produces limited plants. In vitro propagation is a very promising technique for plant propagation because it can produce a lot of plant seeds in a short time. Bulbs are one of the fastest explants for growing shoots in lilies, but it is not known for certain which cuts of explants from bulb scales are best for multiplying in vitro. This study aims to determine the effect of lily bulb explants and the concentration of NAA and BAP growth regulators on the growth of lily bulb explants. The best results were obtained on the base and middle cuts explant of bulb scales compared to the tip cuts explant ones. The best results of the growing percentage, the number of shoots and the best growing time are shown in the combination treatment of growth regulator 1 mg L−1 NAA and 1 mg L−1 BAP. The optimum results on the number of micro bulbs were found in the treatment of growth regulators 0.5 mg L−1 NAA and 1 mg L−1 BAP. The best results of the average time formed micro bulb was in the treatment of 1 mg L−1 NAA and BAP with middle explant cuts, and treatment concentrations of 0.5 mg L−1 NAA and BAP in the base explant section. The base and middle bulb explants are able to regenerate or grow higher shoots. This is caused by the presence of endogenous natural auxin and the spread of auxin in plant parts not in the same amount. Therefore when added to the exogenous growth regulator such as auxin or cytokines to culture media will further trigger the formation of micro tubers more quickly,. It can increase the concentration of endogenous growth regulators in cells, help growing process and developing tissue.   Keywords: Bulb, lily, micro bulbs, in vitro, shoots

scholarly journals Plant Regeneration of Pampas Grass from Immature Inflorescences Cultured in Vitro

HortScience ◽  
1992 ◽  
Vol 27 (7) ◽  
pp. 841-843 ◽  
Author(s):  
C.D. Robacker ◽  
W.L. Corley
Keyword(s):  
Acetic Acid ◽  
Plant Regeneration ◽  
Developmental Stage ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Growth Regulator ◽  
Basal Medium ◽  
Cortaderia Selloana ◽  
Dichlorophenoxy Acetic Acid

A micropropagation system to obtain plants from inflorescences of pampas grass (Cortaderia selloana Schult. `Pumila') was developed. Factors examined included developmental stage of inflorescence cultured and growth regulator combinations and concentrations that support explant establishment, shoot regeneration, and rooting. Immature inflorescences ≈300 mm long formed many shoot primordia when initially cultured on Murashige and Skoog basal medium containing 4.5 μm 2,4-D and 8.9 μm BA and subcultured to medium with 0.4 μm 2,4-D and 4.4 μm BA. Thereafter, monthly transfer to a medium without growth regulators yielded about three shoots per tube per month for more than 6 months. Most shoots rooted spontaneously and were easily hardened to greenhouse conditions. Field-tested plants flowered within 2 years and nearly all appeared identical to the parent cultivar. With this technique, several thousand plants can be obtained from a single inflorescence in 1 year. Chemical names used: N -(phenylmethyl)-1 H -purine-6-amine (BA); (2,4-dichlorophenoxy)acetic acid (2,4-D).

scholarly journals USE OF FORCING SOLUTIONS TO STUDY PLANT GROWTH REGULATOR EFFECTS ON VANHOUTTE'S SPIRAEA CULTURED IN VITRO

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1124e-1124
Author(s):  
Guochen Yang ◽  
P. E. Read
Keyword(s):  
Plant Physiology ◽  
Plant Growth ◽  
Growth Regulators ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Woody Plant ◽  
Shoot Proliferation ◽  
Plant Tissues ◽  
Forcing Solution

Vanhoutte's spiraea has been propagated in vitro using explants from softwood growth of dormant stems forced in a solution containing 200 mg/l 8-hydroxyquinoline citrate (8-HQC) and 2% sucrose (Yang and Read, 1989). Objectives to further utilize this system were to determine the feasibility of applying plant growth regulators (PGR) via the forcing solution to softwood growth from forced dormant stems and to study the resulting influence on in vitro culture. BA and GA3 were placed in the forcing solution at various concentrations, including a zero PGR control. Explants were cultured on Linsmaier and Skoog (LS) medium containing zero PGR or different amounts of BA or thidiazuron (TDZ) or combinations of BA and IAA. Control explants placed on LS medium supplemented with 5uM BA with or without 1 or 5uM IAA, or with 0.5 or 0.75 uM TDZ alone produced the best shoot proliferation. BA in the forcing solution stimulated micropropagation, while GA3 caused less proliferation than explants from control solutions. Forcing solutions containing PGR are useful for manipulating responses of plant tissues cultured in vitro and for studying PGR influence on woody plant physiology.

scholarly journals In Vitro Germination of Native Terrestrial Orchids

HortScience ◽  
1998 ◽  
Vol 33 (4) ◽  
pp. 595e-595
Author(s):  
Ing-Jiun Tom Wu ◽  
G.L. Wheeler ◽  
F.H. Huang
Keyword(s):  
Seed Germination ◽  
Growth Regulators ◽  
Growth Regulator ◽  
Multiple Shoots ◽  
In Vitro Germination ◽  
Flower Buds ◽  
Terrestrial Orchids ◽  
Cypripedium Calceolus ◽  
Single Seeds

Scarification treatments (a control, a 10-minute vacuum, or a 1.5-minute ultrasound), different media (modified Norstog and Van Waes) and growth regulators [benzyladenine (BA) at 0, 1, 1.5, or 2 mg·L-1 and 6-(r,r-dimethylallylamino)-purine riboside (2iPR) at 0, 1, 1.5 or 2 mg·L-1] were used in combination to increase seed germination of Cypripedium calceolus var. parviflorum. Seeds treated with ultrasound had higher germination (58.0%) than those treated with vacuum (27.4%) or controls (19.2%). Germination rates increased with 2iPR level and reached a maximum between 1.5 and 2 mg·L-1. Seeds on Van Waes medium, which were not transferred to fresh medium after germination, had a severe browning problem causing many protocorms to die. Those on Norstog medium continued to grow into seedlings with less browning. Germination rates of Calopogon tuberosus × Calopogon `Adventure' and Liparis liliifolia were determined on the different media and growth regulator treatments. Multiple shoots of Calopogon developed from single seeds on media containing growth regulators. Flower buds formed in vitro on Calopogon in media containing 1 mg·L-1 or higher BA 5 months after germination. L. Iiliifolia seeds in Norstog medium had a higher proportion of germination than those in Van Waes medium.

Micropropagation of Highly Productive Forms of Diploid and Triploid Aspen

2014 ◽  
Vol 962-965 ◽  
pp. 681-690 ◽  
Author(s):  
Dmitriy Nikolaevich Zontikov ◽  
Svetlana Zontikova ◽  
Roman Sergeev ◽  
Alex Shurgin ◽  
M. Sirotina
Keyword(s):  
Growth Regulators ◽  
Source Material ◽  
Soil Conditions ◽  
Nutrient Media ◽  
Clonal Micropropagation ◽  
Planting Material ◽  
Triploid Clones

The stages of getting the planting material for laying plantations of the triploid aspen (Populustremula L.) are illustrated in this work. The source material selected from the genetic fund “The gigantic aspens” is characterized. The peculiarities of using the method of clonal micropropagation when getting the planting material, the usage of a rootstock as a source of donor explants for the introduction into the culture in vitro, the influence of a kind of nutrient media and growth regulators NAA and BAP on the growth and the development of microsprouts. The distinctions in the speed of the growth of the culture in vitro of diploid and triploid clones have been analyzed. The adaptation of the plants-regenerants to the soil-conditions.

An Effective Immunodepressive Treatment for Demonstrating in Allogeneic Mice the Tumorigenic Properties of Cells Grown in Vitro.

1975 ◽  
Vol 61 (3) ◽  
pp. 223-236 ◽  
Author(s):  
Silvio Parodi ◽  
Marco Cavanna ◽  
Renata Finollo ◽  
Giovanni Brambilla ◽  
Ariella Furlani ◽  
...  
Keyword(s):  
Experimental Data ◽  
Tumor Growth ◽  
Intensive Treatment ◽  
Daily Administration ◽  
In Vitro Growth ◽  
Combined Use ◽  
Cells Cultured

Short intensive treatment with N-diazoacetylglycine amide (DGA) before the inoculum, followed by prolonged daily administration of L-aspàraginase (Asnase), was tested for its ability to elicit tumorigenic properties of fibroblast-like cells cultured in vitro. With this treatment progressive tumor growth was obtained in allogeneic mice injected with cells of a transformed subline. Results show that combined use of DGA and Asnase affords a higher probability of proving in vivo the tumorigenic properties of injected cells than in newborn or X-irradiated recipients. Experimental data indicate that L-asparagine depletion does not inhibit the in vitro growth of fibroblast-like cells.

scholarly journals Clonal Micropropagation of representatives of the genus Dactylorhiza Neck. ex Nevski

2021 ◽  
Vol 4 (46) ◽  
pp. 17-17
Author(s):  
Alexander Saakian ◽  
◽  
Keyword(s):  
Survival Rate ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Culture Medium ◽  
Culture Media ◽  
Ms Medium ◽  
Organic Additives ◽  
Clonal Micropropagation ◽  
Half Strength

Abstract The aim of this study is to develop and improve methods of in vitro propagation of representatives of Dactylorhiza: D.baltica , D. fuchsii. For the study, we used protocorms obtained by the asymbiotic germination of seed during 90 days. It has been established that half-strength of Murashige and Skoog (1962) medium (½ MS) supplemented with 1-2 mg/l 6-Benzylaminopurine(6-BAP), potato puree (20g/l), and charcoal (1g/l) effectively influenced the development of protocorms, and seedlings formation in the studied species. The result of the study showed that the survival rate of protocorms was high in all experimental culture media, but in D. fuchsii it was better at a concentration 2mg/l of 6-BAP (95.4%), while in D. baltica it was high at 1mg/l (87.0%). The highest percentage of multiple protocorms (68%) and the formation of new secondary protocorms in D. fuchsii (5,5±0,3 units) were observed on a culture medium containing 2 mg/l 6-BAP. The highest percent of rooting of D. fuchsii protosoms (78%) and length of roots (0.9cm) observed in ½ MS medium without growth regulators. During the development of D. baltica protosoms, the culture medium of ½ MS containing 1 mg/l 6-BAP had the best effect on the number of roots (1.8±0.1root/protosom), while the medium supplemented with 2mg/l of 6-BAP contributed to the formation of a larger number of new secondary protocorms (3,2±0,1protocorm/unit). During the subsequent cultivation of protosoms of D. baltica on a culture medium containing 1 mg/l it was observed an increase in the height of shoots (4,8±0,3 см), and the length of roots (2,2±0,1 см), wherein the number of newly formed protocorms was higher by 30% on the medium supplemented with 2 mg/l 6-BAP. Keywords: DACTYLORHIZA BALTICA, DACTYLORHIZA FUCHSII, IN VITRO, PROTOCORMS, ORGANIC ADDITIVES

scholarly journals In Vitro Optimization of Main Elements of the Grapes Reproduction Process

2019 ◽  
Author(s):  
A A Batukaev ◽  
D O Palaeva ◽  
M S Batukaev ◽  
E A Sobralieva
Keyword(s):  
Growth Regulators ◽  
Plant Reproduction ◽  
New Methods ◽  
Planting Material ◽  
Reproduction Process ◽  
Positive Effect ◽  
Reproduction Factor

New methods for adapting in-vitro grape plants to in vivo conditions were developed. The use of growth regulators (6-BAP, 2iP, kinetin) enhanced rhizogenesis and increased the in-vitro plant reproduction coefficient. Lignohumate had a positive effect on plants and increased the leaf area and plant height 30 days after adaptation (concentration of 1.0 g/l). The use of the developed elements of clonal micro-reproduction and adaptation made it possible to significantly increase the reproduction factor, improve the quality of planting material, reduce costs of plants and increase profitability.

scholarly journals EFFECT OF EXPALNT TYPE AND SOME PLANT GROWTH REGULATORS ON CULTURE INITIATION OF STEVIA PLANTS IN VITRO

2017 ◽  
Vol 48 (5) ◽  
Author(s):  
Khierallah & Al-Obaidy
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Shoot Tips ◽  
Control Treatment ◽  
Ms Medium ◽  
Explant Type ◽  
Culture Initiation ◽  
The Impact

This research was conducted in order to study the effect of explant type and some plant growth regulators on culture initiation of Stevia rebaudiana Bertoni in vitro. The experiments included surface sterilization and test two types of explants (shoot tips and stem nodes) and the impact of KIN and BA and IAA and IBA in the cultures initiation. Results revealed the efficiency of sodium hypochlorite (NaOCl) for disinfestation of explant at 0.050% concentration giving less contamination for shoot tips and stem nods (10% and 20% respectively). Results showed that shoot tips inoculated in MS medium plus KIN at 0.3 mg. L-1 was significantly increase the number of regenerated shoots as it produced 4.2 shoots per explant while medium without cytokinin (control) produced less number of shoots reached 1.4 shoots per explant. KIN treatment reduced shoots length as control treatment produced the highest length (6.74 cm).  The interaction between the explant type and BA concentration was significantly increase the number of regenerated shoots as shoot tips produced 3.6 shoots per explant in MS medium supplemented with 0.1 mg. L-1. BA treatment reduced shoots length as control treatment produced the highest length (6.74 cm). No positive effect was gain when auxins (IBA and IAA) were added in combination with cytokinin in culture medium. The above results can be adopted to established stevia in vitro culture successfully.

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