scholarly journals Product formulation is crucial to the success of lipid-based bio-fungicides

2018 ◽  
Vol 71 ◽  
pp. 272-284 ◽  
Author(s):  
Kirstin Wurms ◽  
Annette Ah Chee

Powdery mildew is a major cause of damage to squash plants. Anhydrous milk fat (AMF) or soybean oil (SBO) may be effective at treating this disease but these active ingredients must be mixed with an emulsifier to enable even distribution and suspension of fat globules, and an antioxidant to prevent rancidity. The overall formulation may affect disease control efficacy, leaf health and product stability. The effect of different emulsifiers and antioxidants on emulsion stability, odour and shelf-life of AMF and SBO bio-fungicides was tested in laboratory assays, and on powdery mildew disease control efficacy and leaf health on glasshouse-grown squash plants. Both AMF and SBO formulations including a polyglycerol ester emulsifier (Grindsted® PGE 20 Veg) resulted in the best emulsion stability, disease control and leaf health. None of the antioxidants tested significantly affected on disease control efficacy in AMF formulations, but SBO formulations containing vitamin E as the antioxidant provided the best disease control efficacy and emulsion stability.

2011 ◽  
Vol 64 ◽  
pp. 201-208 ◽  
Author(s):  
K.V. Wurms ◽  
A. Ah Chee

Powdery mildew (PM) is one of the most serious global diseases of apples roses cucurbits grapes and cereals The potential of anhydrous milk fat (AMF) and soybean oil (SBO) formulations to control apple PM (caused by Podosphaera leucotricha) on the apple cultivar Royal Gala was investigated Potted seedlings in a glasshouse were left unsprayed or subjected to weekly sprays of AMF SBO fungicide (sulphur as Kumulus DF) or water During 67 weeks of treatment the AMF and SBO formulations significantly reduced PM to


LWT ◽  
2019 ◽  
Vol 105 ◽  
pp. 48-56 ◽  
Author(s):  
Fani Th. Mantzouridou ◽  
Eleni Naziri ◽  
Aikaterini Kyriakidou ◽  
Adamantini Paraskevopoulou ◽  
Maria Z. Tsimidou ◽  
...  

2011 ◽  
Vol 64 ◽  
pp. 195-200 ◽  
Author(s):  
A. Ah Chee ◽  
K.V. Wurms ◽  
M. George

Powdery mildew (PM) is a serious fungal disease of a wide range of horticultural crops and can adversely affect crop yield and quality Anhydrous milk fat (AMF) and soybean oil (SBO) emulsions were evaluated for control of PM (Sphaerotheca pannosa var rosae) on potted rose plants (Rosa L sp Splendid Surprise and Sahara) maintained in a controlled environment Foliage was sprayed weekly with AMF (07 w/v) SBO (2 w/v) fungicide (Supershield 1 v/v) water or left unsprayed PM infection increased to 100 leaf area in water and unsprayed control plants over 6 weeks The fungicide reduced infection to a severity rating of 5 (>40 leaf area infection) The AMF and SBO treatments gave significantly better disease control (P


Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 423
Author(s):  
Kirstin V. Wurms ◽  
Annette Ah Chee ◽  
Peter N. Wood ◽  
Joseph T. Taylor ◽  
Frank Parry ◽  
...  

Synthetic controls of crop pathogens are increasingly associated with harm to the environment and human health, and pathogen resistance. Pesticide residues in crops can also act as non-tariff trade barriers. There is therefore a strong imperative to develop biologically based and natural product (NP) biofungicides as more sustainable alternatives for crop pathogen control. We demonstrate the field efficacy, over multiple seasons, of NP biofungicides, NP1 (based on anhydrous milk fat) and NP2 (based on soybean oil), on two major diseases of winegrapes—Botrytis bunch rot (Botrytis) and powdery mildew (PM). The NPs were integrated into a season-long integrated disease management programme that has produced chemical-residue-free wines. Efficacies for Botrytis control on three different varieties were: 63–97% on Chardonnay, 0–96% for Sauvignon Blanc and 46–58% on Riesling; with 65–98% PM control on Chardonnay and Riesling. NP2 exhibited the significant control of Botrytis latent infections, making it a viable alternative to mid-season synthetic fungicides. Disease control was significantly better than the untreated control and usually as efficacious as the synthetic fungicide treatment(s). Yields and wine quality in NP-treated crops were normally equivalent to those in the synthetic fungicide treatments. The results indicate that NP-mediated disease control of Botrytis and powdery mildew can be obtained in the vineyard, without synthetic fungicide input.


2015 ◽  
Vol 68 ◽  
pp. 380-388 ◽  
Author(s):  
K.V. Wurms ◽  
J.D. Hofland-Zijlstra

Powdery mildew (PM) is a very serious disease affecting glasshousegrown roses and tomatoes in the Netherlands Control is limited because of resistance to existing fungicides Anhydrous milk fat (AMF) and soybean oil (SBO) emulsions were evaluated for control of PM in roses and tomatoes Both AMF (14 g/litre) and SBO (14 g/litre) provided powdery mildew control on rose leaves and blooms that was significantly better (P


Dairy ◽  
2021 ◽  
Vol 2 (2) ◽  
pp. 202-217
Author(s):  
Michele Manoni ◽  
Donata Cattaneo ◽  
Sharon Mazzoleni ◽  
Carlotta Giromini ◽  
Antonella Baldi ◽  
...  

Milk lipids are composed of milk fat globules (MFGs) surrounded by the milk fat globule membrane (MFGM). MFGM protects MFGs from coalescence and enzymatic degradation. The milk lipid fraction is a “natural solvent” for macronutrients such as phospholipids, proteins and cholesterol, and micronutrients such as minerals and vitamins. The research focused largely on the polar lipids of MFGM, given their wide bioactive properties. In this review we discussed (i) the composition of MFGM proteome and its variations among species and phases of lactation and (ii) the micronutrient content of human and cow’s milk lipid fraction. The major MFGM proteins are shared among species, but the molecular function and protein expression of MFGM proteins vary among species and phases of lactation. The main minerals in the milk lipid fraction are iron, zinc, copper and calcium, whereas the major vitamins are vitamin A, β-carotene, riboflavin and α-tocopherol. The update and the combination of this knowledge could lead to the exploitation of the MFGM proteome and the milk lipid fraction at nutritional, biological or technological levels. An example is the design of innovative and value-added products, such as MFGM-supplemented infant formulas.


LWT ◽  
2021 ◽  
pp. 111659
Author(s):  
Naiyan Lu ◽  
Jiyue Wang ◽  
Zhe Chen ◽  
Xuan Zhang ◽  
Chen Chen ◽  
...  

Plants ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1496
Author(s):  
Sohyun Bae ◽  
Jae Woo Han ◽  
Quang Le Dang ◽  
Hun Kim ◽  
Gyung Ja Choi

Plants contain a number of bioactive compounds that exhibit antimicrobial activity, which can be recognized as an important source of agrochemicals for plant disease control. In searching for natural alternatives to synthetic fungicides, we found that a methanol extract of the plant species Platycladus orientalis suppressed the disease development of rice blast caused by Magnaporthe oryzae. Through a series of chromatography procedures in combination with activity-guided fractionation, we isolated and identified a total of eleven compounds including four labdane-type diterpenes (1–4), six isopimarane-type diterpenes (5–10), and one sesquiterpene (11). Of the identified compounds, the MIC values of compounds 1, 2, 5 & 6 mixture, 9, and 11 ranged from 100 to 200 μg/mL against M. oryzae, whereas the other compounds were over 200 μg/mL. When rice plants were treated with the antifungal compounds, compounds 1, 2, and 9 effectively suppressed the development of rice blast at all concentrations tested by over 75% compared to the non-treatment control. In addition, a mixture of compounds 5 & 6 that constituted 66% of the P. orientalis ethyl acetate fraction also exhibited a moderate disease control efficacy. Together, our data suggest that the methanol extract of P. orientalis including terpenoid compounds has potential as a crop protection agent.


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