UJI EFEKTIVITAS ANTIMIKROBA PADA EKSTRAK DAUN GAMBIR (UNCARIA GAMBIER ROXB.) DAN DAUN SIRIH HIJAU (PIPER BETLE LINN.) TERHADAP STREPTOCOCCUS MUTANS, ESCHERICHIA COLI DAN CANDIDA ALBICANS

2017 ◽  
Vol 1 (1) ◽  
pp. 064-072
Author(s):  
Reki Wicaksono Ashadi
Keyword(s):  
Escherichia Coli ◽  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Leaf Extract ◽  
Piper Betle ◽  
Minimum Concentration ◽  
Inhibition Of Growth ◽  
Main Components ◽  
Concentration Levels

Gambier contains two main components, namelycatechin and katekutanat acid. According to Lemmens (1999), gambier have three benefits: fortanning leather; as a stimulant as well as medicine. This study aims to determine the concentrationof the inhibition gambier (Uncaria gambier Roxb.) and betelleaf (Piper betle Linn.) as well astocompare the differences in the inhibition of growth of Streptococcusmutans, Escherichiacoli and Yeast Candida albicans. The Results showed that gambier extract (Uncaria gambier Roxb.) and green betelleaf (Piper betle Linn.) indicates that the leaf extract of betelleaf and gambier have no activity on hibiting the growth of Candida albicans. However extract from betelleaf can inhibit the growth of Streptococcus mutans and Escherichia coli with a minimum concentration levels of 25%.

scholarly journals AÇÃO ANTIMICROBIANA DO Zingiber officinalle FRENTE À MICROBIOTA BUCAL

2006 ◽  
Vol 28 (62) ◽  
Author(s):  
Ana Maria Trindade Grégio ◽  
Edilene Sayuri Miyamoto Fortes ◽  
Edvaldo A. Ribeiro Rosa ◽  
Rossana Baggio Simeoni ◽  
Rosemeire Takaki Rosa
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Streptococcus Mutans

A fitoterapia tem sido alvo de investigações científicas, especialmente em países do oriente. No Brasil, também ocorre um especial interesse da comunidade científica quanto ao uso, efeito e propriedades farmacológicas das plantas medicinais, tendo o seu uso direcionado ao tratamento de várias doenças. A microbiota bucal é um dos locais em que há uma maior variedade de microrganismos que podem, por um desequilíbrio, tornar-se patogênicos. Baseado nisso, um estudo do extrato da Zingiber officinalle foi realizado para avaliar o seu potencial antimicrobiano, com microrganismos comumente encontrados na cavidade bucal, no qual foi obtida a concentração mínima inibitória do extrato de gengibre frente a alguns tipos de patógenos presentes na microbiota bucal do ser humano. Foi utilizado o método microbiológico no qual os extratos glicólico e hidroalcoólico do gengibre sofreram diluições seriadas no meio de cultura (BHI) para uma posterior inoculação dos microrganismos, Streptococcus mutans, Staphylococcus aureus, Escherichia coli e Candida albicans. Obteve-se a concentração mínima inibitória dos extratos de gengibre, tanto na sua forma glicólica quanto hidroalcoólica. A dose mínima necessária para inibir o crescimento dos vários microrganismos testados foi de 5mg/ml dos dois extratos estudados. O extrato da Zingiber officinalle apresentou atividade antibacteriana e antifúngica relevante para a Odontologia, podendo contribuir para o tratamento de doenças causadas por esses microrganismos presentes na cavidade bucal.

scholarly journals Uji Aktivitas Antifungi Ekstrak Daun Sirih (Piper betle L) Terhadap Saprolegnia sp. Secara In Vitro [Antifungal Activities Test Of Betel Leaf Extract (Piper betle L) On Saprolegnia sp. By In Vitro]

2019 ◽  
Vol 5 (1) ◽  
pp. 15
Author(s):  
Rahayu Kusdarwati, Pustika Murtinintias, Dewa Ketut Meles
Keyword(s):  
Minimum Inhibitory Concentration ◽  
Leaf Extract ◽  
Inhibitory Concentration ◽  
Piper Betle ◽  
Minimum Concentration ◽  
Minimum Fungicidal Concentration ◽  
Betel Leaf ◽  
Concentration Test ◽  
Fungal Suspension

Abstract Saprolegniasis is a mycotic disease caused by Saprolegnia sp. that usually attacking wild fish and farming fish. Saprolegnia sp. cause a lot of harm in process of the fish cultivation. Prevention and treatment of the common practice is use chemical drugs, but the use of these chemicals tend to be environmentally unfriendly and there are has karsinogenik effect. Therefore, the use of traditional medicines is one of alternative to control Saprolegnia sp. safer than chemical drugs. Green betel leaf contains phenolic compounds and tannins are efficacious as antifungal agent. This study aims to prove the antifungal activity of extracts of betel leaf (Piper betle L) for Saprolegnia sp., and to know the minimum concentration of betel leaf extract (Piper betle L) as antifungal for Saprolegnia sp. The design of this experiment is used completely randomized design (CRD) with 11 treatments and 3 replications. This study used the dilution method through the Minimum Inhibitory Concentration determination (MIC) and Minimum Fungicidal Concentration (MFC). The concentration of the extract used was 50% (0.5 g/ml), 25% (0.25 g/ml), 12.5% (0.125 g/ml), 6.25% (0.0625 g/ml), 3.13% (0.0313 g/ml), 1.56% (0.0156 g/ml), 0.78% (0.0078 g/ml), 0.39% (0.0039 g/ml), 0.2% (0.002 g/ml) of betel leaf extract. A positive control containing 2 ml of 3% hydrogen peroxide were added fungal suspension until 4 ml Negative control containing 2 ml of 10% DMSO were added fungal suspension until 4 ml. The main parameters in this study is the value of optical density (OD) for MIC (Minimum Inhibitory Concentration) test and the absence of Saprolegnia sp. growing on SDA media for MFC (Minimum Fungicidal Concentration) test. 0.20% (0.002g/ml) concentration of of betel leaf extract is the minimum concentration that can inhibit the growth of Saprolegnia sp. MFC test results showed concentrations of 0.78% (0.0078 g/ml) betel leaf extract is the minimum concentration that can kill Saprolegnia sp.

Antibacterial, antibiofilm, and anti-adhesion activities of Piper betle leaf extract against Avian pathogenic Escherichia coli

2021 ◽  
Vol 204 (1) ◽  
Author(s):  
Pawinee Kulnanan ◽  
Julalak Chuprom ◽  
Thotsapol Thomrongsuwannakij ◽  
Chonticha Romyasamit ◽  
Suthinee Sangkanu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Leaf Extract ◽  
Piper Betle ◽  
Avian Pathogenic Escherichia Coli ◽  
Pathogenic Escherichia Coli

scholarly journals Aceite foliar de Ocotea quixos (Lam.) Kosterm.: actividad antimicrobiana y antifúngica

La Granja ◽  
2008 ◽  
Vol 7 (1) ◽  
pp. 3 ◽  
Author(s):  
Paco Noriega ◽  
Cesare Dacarro
Keyword(s):  
Escherichia Coli ◽  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Staphylococcus Epidermidis ◽  
Para Determinar

Los principales componentes identificados en el aceite esencial de las hojas de Ocotea quixos (Lam.) Kosterm. son: Cariofileno 19,029%, Humuleno 14,323% y Eremofileno 11,407%, aclarándose que éstos sobresalen de un total de 62 compuestos. La técnica utilizada para la extracción del aceite fue destilación con agua y vapor de agua, empleando un destilador de 250 litros de capacidad. La cantidad de aceite obtenida fue de 3 ml por cada 10 kg de hojas frescas. El aceite fue utilizado para un experimento de evaluación de actividad antimicrobiana y antifúngica, siendo el resultado la inhibición del crecimiento de cepas de Staphylococcus epidermidis, Staphylcoccus aureus, Escherichia coli, Candida albicans, Streptococos piogenes y Streptococcus mutans. Los resultados del experimento demostraron que el aceite foliar tiene una alta capacidad inhibitoria contra hongos (levaduras) y bacterias; así, se recomienda por un lado, continuar con análisis químicos más finos para determinar la naturaleza específica de los 62 compuestos y, por el otro, realizar ensayos con un número mayor de agentes patógenos.

scholarly journals In-Vitro Evaluation of Antimicrobial Activities of Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, Neisseria gonorrhoeae, and Candida albicans Nosodes

Homeopathy ◽  
2021 ◽  
Author(s):  
Renuka Munshi ◽  
Gitanjali Talele ◽  
Rajesh Shah
Keyword(s):  
Escherichia Coli ◽  
Candida Albicans ◽  
Klebsiella Pneumoniae ◽  
Neisseria Gonorrhoeae ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Positive Control ◽  
E Coli ◽  
Inhibition Of Growth

Abstract Background This study presents the results of the minimum inhibitory concentration (MIC) assay of a series of nosodes: namely Escherichia coli, Klebsiella pneumoniae, Salmonella typhi, Neisseria gonorrhoeae, and Candida albicans. Each was tested against its corresponding infection as well as cross infections. Methods In-vitro efficacy of polyvalent nosodes was tested using the MIC assay technique. The nosodes, namely C. albicans polyvalent nosode (35c, 100c), N. gonorrhoeae (35c), K. pneumoniae (35c, 100c), E. coli polyvalent nosode (35c, 100c) and Salmonella typhi polyvalent nosode (30c, 100c), were tested along with positive and negative controls. Nosodes were studied in different potencies and at 1:1 dilution. Results C. albicans polyvalent nosode 35c, 100c, N. gonorrhoeae 35c, and positive control amphotericin B showed inhibition of the growth of C. albicans species. K. pneumoniae 35c, E. coli polyvalent nosode 100c, and meropenem (positive control) showed inhibition of the growth of K. pneumoniae; this effect was not seen with ceftriaxone, ofloxacin and amoxicillin antibiotics. E. coli polyvalent nosode 30c in 10% alcohol (direct and dilution 1:1) and the positive controls ciprofloxacin, ofloxacin, and amoxicillin showed inhibition of the growth of E. coli. The S. typhi polyvalent nosode 30c in 10% alcohol showed inhibition of growth of S. typhi. Conclusion This study reveals that the tested nosodes exhibited antibacterial potential against the corresponding micro-organisms and against other selected organisms studied using this assay.

scholarly journals Uji Aktivitas Toksisitas Dan Antimikroba Flavonoid Total Daun Benalu (Dendrophthoe pentandra (L) Miq) Dari Pohon Glodokan (Polyalthia longifolia)

2018 ◽  
Vol 3 (1) ◽  
pp. 32-43
Author(s):  
ALIYAH FAHMI ◽  
Rumondang Bulan
Keyword(s):  
Escherichia Coli ◽  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Brine Shrimp ◽  
Artemia Salina ◽  
Lethal Concentration ◽  
Brine Shrimp Lethality ◽  
E Coli ◽  
Polyalthia Longifolia

Penelitian mengenai uji aktivitas toksisitas dan antimikroba flavonoid total daun benalu (Dendrophthoe pentandra (L) Miq) dari pohon glodokan (Polyalthia longifolia) telah dilakukan. Penelitian ini bertujuan untuk mengetahui aktivitas toksisitas dari flavonoid total daun benalu pohon glodokan berdasarkan metode Brine Shrimp Lethality Test (BSLT)  menggunakan larva udang Artemia salina Leach diperoleh Lethal Concentration (LC50) sebesar 30.06 mg/L yang berarti memiliki aktivitas toksisitas yang toksik sementara untuk aktivitas antimikroba dari flavonoid total daun benalu pohon glodokan dengan Metode Difusi Agar diperoleh diameter zona hambat pada Streptococcus  mutans pada konsentrasi sampel flavonoid total pohon glodokan 3%, 6% dan 9 % adalah 6; 9 dan 17.25 mm, pada Escherichia coli adalah 3.55; 4.25 dan 9.15 mm dan pada Candida albicans adalah 8.30; 4 dan 5.30 mm dimana semakin besar konsentrasi maka daya hambat pada S. mutans dan E. coli semakin besar namun pada C. albicans kurang mempengaruhi tetapi memiliki aktivitas hambat yang baik sehingga efektif dikembangkan sebagai zat antimikroba.

scholarly journals Pengaruh Konsentrasi Ekstrak Rimpang Kencur (Kaempferia Galanga L.) terhadap Pertumbuhan Jamur Candida Albicans dengan Metode Dilusi

2019 ◽  
Vol 2 (2) ◽  
pp. 49
Author(s):  
Eka Aprilianti Aprilianti ◽  
Maulidiyah Salim ◽  
Sri Tumpuk
Keyword(s):  
Candida Albicans ◽  
Research Method ◽  
Effective Concentration ◽  
Dilution Method ◽  
Strong Inhibition ◽  
Minimum Concentration ◽  
Extract Concentration ◽  
Inhibition Of Growth ◽  
Kaempferia Galanga ◽  
Root Tuber

Abstract:  The lesser galangar rhizome is the root of stay that is branched off and attached to root tuber. Rhizome lesser galangar partly located on the ground. The active ingredient in rhizome lesser galangar are flavonoids are used as an antifungi and can cure diseases by the fungus Candida albicans. The purpose of this research is to know the effect of lesser galangar rhizome extract concentration (Kaempferia galanga L.) to the growth of Candida albicans fungus with dilution method. The research method is experimental. The sample used is the concentration of kencur rhizome extract consisting of 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%. Result of research from 10 concentrations of lesser galangar rhizome with dilution method can know the lowest of bacteria colonies that is concentration 4%, 5%, 6%, 7%, 8%, 9%, 10% that is 0 colony with 100% percentage while number of colony The highest bacteria is 1% concentration of 85 colonies with the percentage of 20.56%. At 4% concentration is the effective concentration of rhizome extract lesser galangar, because it can be a minimum concentration that provides a very strong inhibition of growth. Spearman’s analysis, obtained value  (p = 0,00 <0,05) then Ha accepted, mean there is influence of rhizome extract concentration (Kaempferia galanga L.) to growth of Candida albicans fungi. Abstrak: Rimpang kencur merupakan akar tinggal yang bercabang halus dan menempel pada umbi akar. Rimpang kencur sebagian lagi terletak di atas tanah. Zat aktif dalam rimpang kencur yaiu flavonoid yang digunakan sebagai anti jamur dan dapat menyembuhkan penyakit oleh jamur Candida albicans. Tujuan penelitian adalah untuk mengetahui pengaruh konsentrasi ekstrak rimpang kencur (Kaempferia galanga L.) terhadap pertumbuhan jamur Candida albicans dengan metode dilusi. Metode penelitian berbentuk eksperimental semu. Sampel yang digunakan adalah konsentrasi ekstrak rimpang kencur yang terdiri dari 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%. Hasil penelitian dari 10 konsentrasi rimpang kencur dengan metode dilusi dapat diketahui jumlah koloni bakteri yang terendah yaitu konsentrasi 4%, 5%, 6%, 7%, 8%, 9%, 10% yaitu sebesar 0 koloni dengan persentase 100% sedangkan jumlah koloni bakteri tertinggi yaitu konsentrasi 1% sebesar 85 koloni dengan persentase 20,56%. Pada konsentrasi 4% merupakan konsentrasi efektif ekstrak rimpang kencur, karena dapat merupakan konsentrasi minimum yang memberikan daya hambat pertumbuhan yang sangat kuat. Analisis Spearman’s, didapatkan nilai (p = 0,00 < 0,05) maka Ha diterima, berarti terdapat pengaruh kon

scholarly journals Antimicrobial activity of Leuconostoc mesenteroides biofilm against different microorganisms

2019 ◽  
Vol 13 (1) ◽  
pp. 63-67
Author(s):  
Asmaa S. Ahmed ◽  
Abdullah Awad
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Leuconostoc Mesenteroides ◽  
Microbial Pathogens ◽  
Diffusion Method ◽  
Bacillus Species ◽  
Zone Of Inhibition ◽  
Salmonella Spp

Back ground: Leuconostoc is one of the species of lactic acid bacteria that produced biofilms.Probiotic bacteria that produced biofilm  has  been used as naturopathy  against different microbial pathogens. Objective: This study was conducted to determine the antimicrobial activity of  Leuconostoc  biofilm , against 24 isolates (4 of 6 different types) of food borne pathogens including Staphylococcus aureus , Salmonella spp, Escherichia coli , Klebsiella spp , Pseudomonas aeruginosa , Streptococcus mutans , Bacillus subtillus , Bacillus cereus, Bacillus sterothermophillus and Candida albicans. Materials and methods: using various concentration in vitro by filter paper disk diffusion method . Result: The present study showed the potent antimicrobial activity of the Leuconostoc mesenteroides biofilm against the all tested bacterial pathogens except  Bacillus species and yeast Candida albicans. Biofilm produced by Leuconostoc mesenteroides  showed highest zone of inhibition (13mm) against  Escherichia coli  and lowest zone of inhibition (7.0mm) against Streptococcus mutans . Conclusion: Consequently, Leuconostoc mesenteroides biofilm may be used as an antimicrobial agent in food products to prevent spoilage.

scholarly journals Análisis de la composición del aceite esencial de Myrcianthes rhopaloides (Kunth in H.B.K.) McVaugh, Myrtaceae, y evaluación de su actividad biológica

La Granja ◽  
2007 ◽  
Vol 6 (2) ◽  
pp. 17 ◽  
Author(s):  
María Elena Maldonado ◽  
Cesare Dacarro
Keyword(s):  
Escherichia Coli ◽  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Streptococcus Pyogenes

<p>El estudio de la composición química del aceite esencial de la planta reconocida con el nombre vernáculo de arrayán y que pertenece a la especie Myrcianthes rhopaloides (Kunth in H.B.K.) McVaugh, familia Myrtaceae, fue realizado mediante la técnica de cromatografía de gases acoplada a masas, la que permitió identificar una mezcla de componentes mono y sesquiterpenos. Es así, que los principales compuestos que se destacaron fueron Linalol, Eucaliptol, D –Limoneno y Terpineol; seguidos, en menor proporción, de 4 Terpineol, cis Mirtanol, Cariofileno, ·-Cariofileno, Cadineno y Espatulenol.</p><p>El efecto de la actividad antimicrobiana del aceite de arrayán evidenció una acción fuerte sobre microorganismos como Streptococcus mutans y Streptococcus pyogenes, menor sobre Staphilococcus aureus y Staphilococcus epidermidis, y ausente sobre Escherichia coli y Candida albicans.</p>

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