scholarly journals EVALUASI COATING MATERIALS UNTUK MELINDUNGI BAKTERI PROBIOTIK PSEUDOALTEROMONAS PISCICIDA SELAMA PROSES MIKROENKAPSULASI DAN PENYIMPANAN

2021 ◽  
Vol 7 (2) ◽  
pp. 52-61
Author(s):  
DIAN EKA RAMADHANI
Keyword(s):  
Whey Protein ◽  
Freeze Drying ◽  
Pacific White Shrimp ◽  
Probiotic Bacteria ◽  
White Shrimp ◽  
Economic Losses ◽  
Coating Materials ◽  
Single Coating ◽  
Double Coating

Disease is an obstacle in Pacific white shrimp culture that can cause economic losses. Probiotic bacteria Pseudalteromonas piscicida 1Ub RfR is able to improve the growth performance and immune responses of Pacific white shrimp and has the potential to be developed into dry products to make it more practical for use in the field. This study aims to obtain the best coating materials that can protect probiotic bacteria during the microencapsulation and storage process. This research was conducted in September-December 2020 at the Laboratory of Microbiology of IPB Sukabumi Campus and South-East Asia Food and Agricultural Science and Technology (SEAFAST). This research consists of 2 Chapters, Chapter 1 to obtain the best coating materials and Chapter 2 to obtain the best results after the microencapsulation process. Coating materials used in this study were whey protein and maltodextrin. The microencapsulation technique used is freeze drying and spray drying. The probiotic bacteria used was P. piscicida from Fish Health and Management Laboratory, Department of Aquaculture, FPIK IPB and was marked with rifampicin resistance (1Ub RfR). The research in Chapter 1 consisted of 4 treatments, including K (without coating material), A (single coating with whey protein), B (single coating with maltodextrin), and C (double coating with whey protein and maltodextrin). Furthermore, each treatment in Chapter 1 is continued for the microencapsulation process. The results showed that the treatment with double coating and encapsulated by freeze drying was the best probiotic products compared other treatments.

Use of Cold Atmospheric Plasma To Preserve the Quality of White Shrimp (Litopenaeus vannamei)

2019 ◽  
Vol 82 (7) ◽  
pp. 1217-1223 ◽  
Author(s):  
DE ANGELIS de SOUZA SILVA ◽  
MARIA CARLA da SILVA CAMPELO ◽  
LUCAS de OLIVEIRA SOARES REBOUÇAS ◽  
JUSSIER de OLIVEIRA VITORIANO ◽  
CLODOMIRO ALVES ◽  
...  
Keyword(s):  
Shelf Life ◽  
Litopenaeus Vannamei ◽  
Cold Plasma ◽  
Bacterial Load ◽  
Pacific White Shrimp ◽  
Atmospheric Plasma ◽  
White Shrimp ◽  
Enzymatic Oxidation ◽  
Economic Losses ◽  
Control Group

ABSTRACT The enzymatic oxidation process that causes black spots on shrimp, named melanosis, leads to spoilage and economic losses. Therefore, there is an urgent need for strategies to reduce melanosis in the food industry. Cold plasma is an emerging nonthermal food processing technology. This work explores a novel application of cold plasma for the preservation of shrimp quality. This study aimed to evaluate the effects of cold plasma on the quality attributes of Pacific white shrimp (Litopenaeus vannamei). The shrimp samples were divided in two groups: the control group and the plasma group. Samples were exposed to nonthermal plasma for 10 min at a frequency of 500 Hz. Microbiological assays, including total counts of mesophilic and psychrotrophic bacteria, Staphylococcus spp., and Salmonella sp., were performed along with investigations of physicochemical parameters, such as pH, color, water-holding capacity, cooking loss, and shear force. In addition, sensory (quality index method) tests were conducted. The plasma group exhibited a shelf life of 14.07 days, while the control group exhibited a shelf life of 9.78 days. The experimental protocol used in this study was not enough to obtain significant reduction in the bacterial load. However, treatment with the cold plasma contributed to improving the physicochemical properties during storage. HIGHLIGHTS

scholarly journals Microencapsulation of probiotics and its applications with prebiotic in Pacific white shrimp larvae through Artemia sp.

2019 ◽  
Vol 18 (2) ◽  
pp. 130-140
Author(s):  
Dian Eka Ramadhani ◽  
Widanarni Widanarni ◽  
Sukenda Sukenda
Keyword(s):  
Disease Resistance ◽  
Immune Responses ◽  
Population Growth ◽  
Freeze Drying ◽  
Vibrio Harveyi ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Immersion Method ◽  
Mannan Oligosaccharide ◽  
Growth Performances

ABSTRACTThis study aimed to produce microencapsulated probiotic Pseudoalteromonas piscicida (1Ub) and evaluate it with preb­iotic mannan-oligosaccharide (MOS) through the enrichment of Artemia sp., on bacterial population, growth performances, immune responses, and disease resistance of Pacific white shrimp larvae. Microencapsulation of probiotic was done by the freeze-drying method. The shrimp larvae were reared for 13 days and fed by the Artemia sp. enriched with microcapsule of probiotic 1Ub (10 g/L), prebiotic MOS (12 mg/L), synbiotic, and control without administration of microencapsulated probiotic and prebiotic, including negative (C-) and positive (C+) control. On the day 14, all of the experimental shrimp larvae except C- were challenged through immersion method with Vibrio harveyi MR5339 (107 CFU/mL). This study showed that the administration of microcapsule of probiotic 1Ub, prebiotic MOS, and synbiotic through the enrichment of Artemia sp. could increase the bacteria population, growth performances, immune responses, and disease resistance of Pacific white shrimp larvae. Moreover, synbiotic treatment demonstrated the best result compared to other treatments.Keywords: probiotic, prebiotic, synbiotic, Pacific white shrimp, microencapsulation ABSTRAKPenelitian ini bertujuan untuk membuat mikrokapsul probiotik Pseudoalteromonas piscicida (1Ub) dan mengevaluasinya dengan prebiotik mannan-oligosaccharides (MOS) melalui pengayaan Artemia sp. terhadap populasi bakteri, performa pertumbuhan, respons imun dan resistensi penyakit pada larva udang vaname. Mikroenkapsulasi probiotik dilakukan dengan metode freeze-drying. Larva udang dipelihara selama 13 hari dan diberi pakan Artemia sp. yang telah diperkaya dengan mikrokapsul probiotik 1Ub (10 g/L), prebiotik MOS (12 mg/L), sinbiotik, dan kontrol tanpa penambahan mikrokapsul probiotik dan prebiotik, termasuk kontrol negatif (C-) dan positif (C+). Pada hari ke-14, seluruh larva udang percobaan kecuali C- diuji tantang melalui metode perendaman dengan Vibrio harveyi MR5339 (107 CFU/mL). Hasil penelitian menunjukkan bahwa pemberian mikrokapsul probiotik 1Ub, prebiotik MOS, dan sinbiotik melalui pengayaan Artemia sp. dapat meningkatkan populasi bakteri, performa pertumbuhan, respons imun, dan resistensi penyakit pada larva udang vaname. Selain itu, perlakuan sinbiotik menunjukkan hasil terbaik dibandingkan perlakukan lainnya.Kata kunci : probiotik, prebiotik, sinbiotik, udang vaname, mikroenkapsulasi

scholarly journals Efficacy of Probiotics and Bioflocs on the Production Performance of Tiger Shrimp Penaeus monodon and Pacific White Shrimp Litopenaeus vannamei during Polyculture Operation

2021 ◽  
pp. 15-28
Author(s):  
K. Sirisha ◽  
Y. Aparna ◽  
M. Srinivasulu Reddy
Keyword(s):  
Growth Rates ◽  
Litopenaeus Vannamei ◽  
Penaeus Monodon ◽  
Pacific White Shrimp ◽  
Mass Mortality ◽  
Shrimp Farming ◽  
Probiotic Bacteria ◽  
White Shrimp ◽  
Production Rates ◽  
Shrimp Culture

Shrimp culture has been developed during the last three decades and the production of farmed shrimp reached its peak. However, the shrimp production was decreased all over the World including in Asian Countries because of the mass mortality due to the outbreak of several diseases predominantly White Spot Syndrome Virus (WSSV) which caused extensive economic damage to the shrimp culture industry. Various Polyculture technologies of shrimp with shellfish, finfish or seaweeds have been implemented to reduce economic damages by mass mortality of shrimp. The present study was conducted with Tiger shrimp Penaeus monodon and Pacific white shrimp Litopenaeus vannamei through Polyculture operation to determine optimum stocking ratios for induction of best performance and highest production rates. Initially, the experiments were conducted with different ratios and combinations of stocking densities 1:1, 2:1 and 1:2 ratios of Penaeus monodon and Litopenaeus vannamei with control group feed with commercial feed, the other group certain Probiotic bacteria such as Bacillus licheniformis and Lactobacillus rhamnosus added in feed, the third group both the Probiotic bacteria and the addition of external Carbon source from sugarcane molasses in the form of Bioflocs for 100 days. To ascertain further interaction of ratios influence, 20:10, 20:12, 20:15 ratios of stocking and Monoculture experiments were conducted. In both the experiments the Survival rate, Final body weights, Weight Gain, Feed conversion ratio, Specific growth rates, Average daily growth rates, Protein efficiency ratio, and Productivity rates were significantly different (P<0.05) in all the different treatments both for P. monodon and L. vannamei. From the results obtained 20:10 ratio of P. monodon and L. vannamei was considered as the best ratio of stocking, which yielded the highest production rates in all the Control, Probiotic added and both Probiotic & Biofloc added groups. The results obtained in the present study clearly demonstrate that the rearing of taxonomically similar species with optimum stocking ratios seems to improve the efficiency of shrimp farming and substantially increasing the production rates. So, this polyculture of P. monodon and L. vannamei can be considered as an alternative approach towards the establishment of sustainable shrimp farming activity which will yield good economic returns.

scholarly journals Microalgae and probiotic bacteria as biofloc inducers in a hyper-intensive Pacific white shrimp (Penaeus vannamei) culture

2021 ◽  
Vol 49 (1) ◽  
pp. 155-168
Author(s):  
Francisco J. Jiménez-Ordaz ◽  
Marco A. Cadena-Roa ◽  
Juan M. Pacheco-Vega ◽  
Maurilia Rojas-Contreras ◽  
Dariel Tovar-Ramírez ◽  
...  
Keyword(s):  
Dry Weight ◽  
Pacific White Shrimp ◽  
Probiotic Bacteria ◽  
White Shrimp ◽  
Ammonium Concentration ◽  
Penaeus Vannamei ◽  
Feed Conversion ◽  
Shrimp Culture ◽  
Commercial Feed ◽  
Significant Difference

Biofloc systems in Pacific white shrimp (Penaeus vannamei) culture generate floccules that remove wastes, and hence, biofloc formation is promoted for shrimp production. This study evaluated the induction of biofloc formation using microalgae and probiotic bacteria in hyper-intensive Pacific white shrimp culture. The experiment was performed for six weeks in tanks (28,000 L each) stocked 350 ind m-3 and weight of 1400 ± 30 mg. Three treatments were assessed: 1) with two diatoms: Grammatophora sp. and Navicula sp., 2) with Navicula sp., and 3) without diatoms. All treatments were added with Schizochytrium sp. and Lactobacillus fermentum TD19. The following parameters were monitored during culture: ammonium, nitrites, nitrates, environmental variables, floccules volume, microorganism presence, the bromatological composition of biofloc, and growth performance parameters of P. vannamei. Our results indicated that the three induced biofloc presented a similar volume (8.34 ± 4.8 mL L-1) and ammonium concentration (<1.0 mg L-1). Treatment 1 generated floccules with the highest concentration of ciliates, rotifers, nematodes, lactic acid bacteria, and the lowest concentration of Vibrio spp. (1.2×103 UFC mL-1), evidencing a significant difference in bromatological composition, with the highest protein and lipid values (28.12 ± 0.50 and 22.44 ± 0.80% biofloc dry weight, respectively) and the best feed conversion ratio (0.89). Based on these results, we suggest that Schizochytrium sp., L. fermentum, and two diatoms should be used to induce biofloc in a hyper-intensive culture of P. vannmaei in order to provide a supplementary nutritional intake, which can constitute an advantage to reduce commercial feed rations.

A SNP Genetic Map for Pacific White Shrimp (Litopenaeus vannamei)

2010 ◽  
Author(s):  
Zhi-Qiang Du ◽  
Suneel K. Onteru ◽  
Danielle Gorbach ◽  
Max F. Rothschild
Keyword(s):  
Genetic Map ◽  
Litopenaeus Vannamei ◽  
Pacific White Shrimp ◽  
White Shrimp

Effects of conjugated linoleic acid and curcumin on growth performance and oxidative stress enzymes in juvenile Pacific white shrimp ( Litopenaeus vannamei ) feed with aflatoxins

2019 ◽  
Vol 51 (3) ◽  
pp. 1051-1060
Author(s):  
Oscar Daniel García‐Pérez ◽  
Mireya Tapia‐Salazar ◽  
Martha G. Nieto‐López ◽  
Julio César Cruz‐Valdez ◽  
Maribel Maldonado-Muñiz ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Linoleic Acid ◽  
Growth Performance ◽  
Conjugated Linoleic Acid ◽  
Litopenaeus Vannamei ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Oxidative Stress Enzymes ◽  
Stress Enzymes ◽  
And Oxidative Stress
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