Determination of Sugar contents, Total Phenol and Antioxidant Activity of various parts ‘Uzun’ pistachio cultivar (Pistacia vera L.)

Objective: The purpose of this study was to evaluated the quality parameters and analyzed the antioxidant activity of seroja leaves Nelumbo nucifera Gaertn. Methods: The quantification of the chemical compound was determined by its total phenol and flavonoid levels. The evaluate the antioxidant activity was determined by the comparability of the four common radical scavenging assays using 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS); 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical; cupric ion reducing antioxidant capacity (CUPRAC); ferric reducing antioxidant power (FRAP); and 2-thiobarbituric acid (TBA) methods. Results: The results of phytochemical screening for simplicia powder and 70% ethanol extract of seroja leaves contain secondary metabolites of alkaloids, flavonoids, saponins, tannins, coumarin, quinones, and triterpenoid steroids. The results of the determination of the quality parameters meet the requirements of quality and safety standard of the medicinal herb. The result of the determination of total phenol content from 70% ethanol extract of Seroja leaves was 181.62±0.82 mg GAE/g extract. The results of the determination of total flavonoid levels from 70% ethanol extract of seroja leaves amounted to 289.83±1.04 mg QE/g extract. The results of antioxidant activity tests using the ABTS, DPPH, and TBA methods showed IC50 respectively 287.7 mg/l, 22.3 mg/l, and 352.6 mg/l and CUPRAC and FRAP methods had an antioxidant capacity of 160.76±0.35 and 253.36±0.48 mg AAE/g extract. Conclusion: Seroja leaves (Nelumbo nucifera Gaertn.) have the potential to be used as an antioxidant medicinal herb and its extract meet the standard of quality control and safety.

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DETERMINATION OF TOTAL PHENOL, FLAVONOID, ANTIOXIDANT ACTIVITY OF OIL PALM LEAVES EXTRACTS AND THEIR APPLICATION IN TRANSPARENT SOAP

Journal of Oil Palm Research ◽

10.21894/jopr.2018.0010 ◽

2018 ◽

Cited By ~ 1

Author(s):

NORASHIKIN AHMAD

Keyword(s):

Antioxidant Activity ◽

Oil Palm ◽

Total Phenol

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Penentuan Total Fenolik, Uji Antioksidan, Dan Uji Antibakteri Pada Ekstrak Etanol Jantung Pisang Ambon (Musa acuminate Colla)

Prosiding Seminar Nasional Kesehatan ◽

10.48144/prosiding.v1i.951 ◽

2021 ◽

Vol 1 ◽

pp. 1903-1914

Author(s):

Ratna Tri Lestari ◽

S Slamet ◽

W Wirasti ◽

Urmatul Waznah

Keyword(s):

Antioxidant Activity ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Total Phenol ◽

Antioxidant Compounds ◽

Antimicrobial Function ◽

High Antioxidant Activity ◽

Banana Flower ◽

Paper Disc

AbstractBanana plant is one type of plant that contains chemical compounds that can be used for treatment. One part of the plant that has not been fully utilized is the heart of the Ambon banana (Musa acuminate Colla). Banana flower is known to contain flavonoid compounds, phenols, alkaloids, tannins, saponins, and coumarins. One of the compounds belonging to the flavonoid group which generally includes natural antioxidant compounds and has an antimicrobial function is phenolic compounds. The purpose of this study was to determine the total phenols, to examine the activity of antioxidants, antibacterials, and the ethanol extract of Ambon banana flower (Musa acuminate Colla). Determination of total phenol using the GAE (Gallic Acid Equivalence) method, antioxidant analysis using the FRAP method, and the antibacterial activity test using the paper disc method. The total phenol yield obtained was 17.7291 ± 0.102 mg GAE/g extract, the results from the antioxidant test were obtained with an EC50 value of 0.0628 µmolFe2+/g sampel extract, which means that the ethanolic extract of Ambon banana flower has a high antioxidant activity value, and antibacterial results in the extract. with a medium average (5-10 mm). From the results of tests carried out, the ethanol extract of Ambon banana flower has antioxidant activity and has little antibacterial power.Keywords : Banana plants; Ambon banana flower; antioxidants; antibacterial; total phenol AbstrakTanaman pisang merupakan salah satu jenis tanaman yang mengandung senyawa kimia yang dapat digunakan untuk pengobatan. Salah satu bagian tanaman yang belum dimanfaatkan secara maksimal adalah jantung pisang ambon (Musa acuminate Colla). Jantung pisang diketahui mengandung senyawa flavonoid, fenol, alkaloid, tannin, saponin, dan kumarin. Salah satu senyawa yang termasuk golongan flavonoid yang umumnya termasuk senyawa antioksidan alami dan memiliki fungsi antimikroba yaitu senyawa fenolik. Tujuan dari penelitian ini yaitu penentuan total fenol, meneliti aktivitas antioksidan, antibakteri, dan pada ekstrak etanol jantung pisang ambon (Musa acuminate Colla). Penentuan total fenol menggunakan metode GAE (Equivalensi Asam Galat), analisis antioksidan menggunakan metode FRAP, dan uji aktivitas antibakteri menggunakan metode kertas cakram. Hasil total fenol yang diperoleh 17,7291 ± 0,102 mg GAE/g ekstrak, hasil dari uji antioksidan didapat dengan nilai EC50 pada ekstrak 0,0628 µmolFe2+/g sampel yang berarti ekstrak etanol jantung pisang ambon mempunyai nilai aktivitas antioksidan yang tinggi, dan hasil antibakteri pada ekstrak dengan rata-rata sedang (5-10 mm). Dari hasil pengujian yang dilakukan ekstrak etanol jantung pisang ambon mempunyai aktivitas antioksidan dan mempunyai sedikit daya antibakteri. Kata kunci : Tanaman pisang; jantung pisang ambon; antioksidan; antibakteri; total fenol.

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Total Phenol and Antioxidant Activity of Ethanol Extract and Water Extract from Claw Uncariaa gambir Roxb

BERKALA SAINSTEK ◽

10.19184/bst.v9i3.27179 ◽

2021 ◽

Vol 9 (3) ◽

pp. 131

Author(s):

Lucky Hartanti ◽

Asri Mulya Ashari ◽

Warsidah Warsidah

Keyword(s):

Antioxidant Activity ◽

Aqueous Extract ◽

Water Extract ◽

Ethanol Extract ◽

Total Phenol ◽

Total Phenolic ◽

Market Demand ◽

University Campus ◽

Industrial Plants

Uncaria gambier Roxb is a plant from the Rubiaceae family, belongs to the Bajakah group and has been used empirically in the treatment of various types of diseases in the community. Besides being one of the industrial plants and export commodities produced in Sumatra and Kalimantan, gambier leaves is also used frequently in Indonesia, both for the pharmaceutical and cosmetic industries. As a result, market demand both nationally and internationally have increased. The aim of this research are determination of antioxidant activity of ethanol extract and aqueous extract of gambir claw using 1,1-diphenil-2-picrylhydrazyl (DPPH) and determination of total phenol using Calcetau folin reagent. Gambier plant samples were taken in the forest area around the Tanjungpura University campus, the claws were separated and dried at room temperature, then powdered and extracted by maceration using 70% ethanol pa and aquadest, then the extract was concentrated until it was ready to be tested for antioxidant activity and determination of total phenol. The total phenolic ethanol extract and aqueous extract of gambir claw were 224.66 mg GAE/g extract (GEA : Gallic Acid Eqivalent) and 299.08 mg GAE/g extract, respectively, while the antioxidant potential (IC50) was 39.566 μg/mL and 65.140 μg/mL, and the IC50 for comparison of Vitamin C was 7.02 μg/mL.

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