scholarly journals Penentuan Total Fenolik, Uji Antioksidan, Dan Uji Antibakteri Pada Ekstrak Etanol Jantung Pisang Ambon (Musa acuminate Colla)

2021 ◽  
Vol 1 ◽  
pp. 1903-1914
Author(s):  
Ratna Tri Lestari ◽  
S Slamet ◽  
W Wirasti ◽  
Urmatul Waznah
Keyword(s):  
Antioxidant Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Total Phenol ◽  
Antioxidant Compounds ◽  
Antimicrobial Function ◽  
High Antioxidant Activity ◽  
Banana Flower ◽  
Paper Disc

AbstractBanana plant is one type of plant that contains chemical compounds that can be used for treatment. One part of the plant that has not been fully utilized is the heart of the Ambon banana (Musa acuminate Colla). Banana flower is known to contain flavonoid compounds, phenols, alkaloids, tannins, saponins, and coumarins. One of the compounds belonging to the flavonoid group which generally includes natural antioxidant compounds and has an antimicrobial function is phenolic compounds. The purpose of this study was to determine the total phenols, to examine the activity of antioxidants, antibacterials, and the ethanol extract of Ambon banana flower (Musa acuminate Colla). Determination of total phenol using the GAE (Gallic Acid Equivalence) method, antioxidant analysis using the FRAP method, and the antibacterial activity test using the paper disc method. The total phenol yield obtained was 17.7291 ± 0.102 mg GAE/g extract, the results from the antioxidant test were obtained with an EC50 value of 0.0628 µmolFe2+/g sampel extract, which means that the ethanolic extract of Ambon banana flower has a high antioxidant activity value, and antibacterial results in the extract. with a medium average (5-10 mm). From the results of tests carried out, the ethanol extract of Ambon banana flower has antioxidant activity and has little antibacterial power.Keywords : Banana plants; Ambon banana flower; antioxidants; antibacterial; total phenol AbstrakTanaman pisang merupakan salah satu jenis tanaman yang mengandung senyawa kimia yang dapat digunakan untuk pengobatan. Salah satu bagian tanaman yang belum dimanfaatkan secara maksimal adalah jantung pisang ambon (Musa acuminate Colla). Jantung pisang diketahui mengandung senyawa flavonoid, fenol, alkaloid, tannin, saponin, dan kumarin. Salah satu senyawa yang termasuk golongan flavonoid yang umumnya termasuk senyawa antioksidan alami dan memiliki fungsi antimikroba yaitu senyawa fenolik. Tujuan dari penelitian ini yaitu penentuan total fenol, meneliti aktivitas antioksidan, antibakteri, dan pada ekstrak etanol jantung pisang ambon (Musa acuminate Colla). Penentuan total fenol menggunakan metode GAE (Equivalensi Asam Galat), analisis antioksidan menggunakan metode FRAP, dan uji aktivitas antibakteri menggunakan metode kertas cakram. Hasil total fenol yang diperoleh 17,7291 ± 0,102 mg GAE/g ekstrak, hasil dari uji antioksidan didapat dengan nilai EC50 pada ekstrak 0,0628 µmolFe2+/g sampel yang berarti ekstrak etanol jantung pisang ambon mempunyai nilai aktivitas antioksidan yang tinggi, dan hasil antibakteri pada ekstrak dengan rata-rata sedang (5-10 mm). Dari hasil pengujian yang dilakukan ekstrak etanol jantung pisang ambon mempunyai aktivitas antioksidan dan mempunyai sedikit daya antibakteri. Kata kunci : Tanaman pisang; jantung pisang ambon; antioksidan; antibakteri; total fenol.

scholarly journals Determination of phenolic contents and antioxidant activity test of ethanol extract of Sirih merah (Piper crocatum Ruiz & Pav.) leaves using the DPPH method

2021 ◽  
Vol 13 (2) ◽  
pp. 137-142
Author(s):  
Kasta Gurning ◽  
◽  
Sifikal Lumbangaol ◽  
Risanti F. R. Situmorang ◽  
Saronom Silaban ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Extraction Process ◽  
Total Phenolic ◽  
Betel Leaf ◽  
Dpph Method

The research objectives were to identify the secondary metabolite components, total phenolic content and determine the antioxidant activity of the ethanol extract of red betel leaf (Piper crocatum Ruiz & Pav.). The extraction process was carried out by materation using ethanol as a solvent. Determination of total phenolic content was carried out colorimetrically with Folin-Ciocalteu reagent measured at a maximum wavelength of 765 nm. Determination of antioxidant activity using the DPPH method measured by spectrophotometry at a maximum wavelength of 517 nm. The results of phytochemical screening of the ethanolic extract of red betel leaf contain secondary metabolites, including flavonoid, phenolic, tannin, alkaloids, steroids, and triterpenoids. The total phenolic content of the red betel leaf ethanol extract was 0.949±0.003 mg GAE/g d.w. and has antioxidant activity (IC50) 84,656 including strong category as an antioxidant. Keywords: Piper crocatum Ruiz & Pav., Antioxidant, Ethanol extract, Folin-Ciocalteu and DPPH

scholarly journals DETERMINATION OF QUALITY PARAMETERS AND TEST ANTIOXIDANT ACTIVITIES OF 70% ETHANOL EXTRACT OF SEROJA LEAVES (NELUMBO NUCIFERA GAERTN.)

2021 ◽  
pp. 30-33
Author(s):  
RATNA DJAMIL ◽  
DIAH KARTIKA PRATAMI ◽  
FEBI AYU PUTRI ◽  
THALIA BREBA OCTAVIA
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Quality Parameters ◽  
Medicinal Herb ◽  
Nelumbo Nucifera ◽  
Total Phenol ◽  
Antioxidant Power

Objective: The purpose of this study was to evaluated the quality parameters and analyzed the antioxidant activity of seroja leaves Nelumbo nucifera Gaertn. Methods: The quantification of the chemical compound was determined by its total phenol and flavonoid levels. The evaluate the antioxidant activity was determined by the comparability of the four common radical scavenging assays using 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS); 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical; cupric ion reducing antioxidant capacity (CUPRAC); ferric reducing antioxidant power (FRAP); and 2-thiobarbituric acid (TBA) methods. Results: The results of phytochemical screening for simplicia powder and 70% ethanol extract of seroja leaves contain secondary metabolites of alkaloids, flavonoids, saponins, tannins, coumarin, quinones, and triterpenoid steroids. The results of the determination of the quality parameters meet the requirements of quality and safety standard of the medicinal herb. The result of the determination of total phenol content from 70% ethanol extract of Seroja leaves was 181.62±0.82 mg GAE/g extract. The results of the determination of total flavonoid levels from 70% ethanol extract of seroja leaves amounted to 289.83±1.04 mg QE/g extract. The results of antioxidant activity tests using the ABTS, DPPH, and TBA methods showed IC50 respectively 287.7 mg/l, 22.3 mg/l, and 352.6 mg/l and CUPRAC and FRAP methods had an antioxidant capacity of 160.76±0.35 and 253.36±0.48 mg AAE/g extract. Conclusion: Seroja leaves (Nelumbo nucifera Gaertn.) have the potential to be used as an antioxidant medicinal herb and its extract meet the standard of quality control and safety.

scholarly journals Total Phenol and Antioxidant Activity of Ethanol Extract and Water Extract from Claw Uncariaa gambir Roxb

2021 ◽  
Vol 9 (3) ◽  
pp. 131
Author(s):  
Lucky Hartanti ◽  
Asri Mulya Ashari ◽  
Warsidah Warsidah
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Water Extract ◽  
Ethanol Extract ◽  
Total Phenol ◽  
Total Phenolic ◽  
Market Demand ◽  
University Campus ◽  
Industrial Plants

Uncaria gambier Roxb is a plant from the Rubiaceae family, belongs to the Bajakah group and has been used empirically in the treatment of various types of diseases in the community. Besides being one of the industrial plants and export commodities produced in Sumatra and Kalimantan, gambier leaves is also used frequently in Indonesia, both for the pharmaceutical and cosmetic industries. As a result, market demand both nationally and internationally have increased. The aim of this research are determination of antioxidant activity of ethanol extract and aqueous extract of gambir claw using 1,1-diphenil-2-picrylhydrazyl (DPPH) and determination of total phenol using Calcetau folin reagent. Gambier plant samples were taken in the forest area around the Tanjungpura University campus, the claws were separated and dried at room temperature, then powdered and extracted by maceration using 70% ethanol pa and aquadest, then the extract was concentrated until it was ready to be tested for antioxidant activity and determination of total phenol. The total phenolic ethanol extract and aqueous extract of gambir claw were 224.66 mg GAE/g extract (GEA : Gallic Acid Eqivalent) and 299.08 mg GAE/g extract, respectively, while the antioxidant potential (IC50) was 39.566 μg/mL and 65.140 μg/mL, and the IC50 for comparison of Vitamin C was 7.02 μg/mL.

scholarly journals Acceptability and Antioxidant Activity Level of Shredded Banana Flower-Chicken Meat

2020 ◽  
Vol 4 (4) ◽  
pp. 299
Author(s):  
Novidiyanto Novidiyanto ◽  
Ori Pertami Enardi ◽  
Ade Devriany ◽  
Auronita Puspa Pratiwi ◽  
Musrifa Airuni
Keyword(s):  
Antioxidant Activity ◽  
Vegetable Consumption ◽  
Chicken Meat ◽  
Activity Level ◽  
Antioxidant Compounds ◽  
Average Amount ◽  
Significant Difference ◽  
Background Data ◽  
High Antioxidant Activity ◽  
Banana Flower

Background: Data Riskesdas 2013 shows that the average amount portion of vegetable consumption in people aged 10 years or older is only 0.5 portions while the vegetable consumption category is said to be "sufficient" is at least 5 portions per day for 7 days a week. Banana blossom is a vegetable and commonly found in Bangka Belitung Islands Province. Banana blossom processing as a source of vegetables rich in antioxidant compounds and chicken meat as a source of animal protein, needs to be done by processing into shredded banana blossom-chicken meat.Objectives: To determine the acceptability and antioxidant activity level of shredded banana blossom-chicken meat.Methods: This research is true experimental. Shredded banana blossom-chicken meat, made using 3 treatment, banana blossom 75%:chicken meat 25% (F1), banana blossom 50%: chicken meat 50% (F2), and banana blossom 25%:chicken meat 75% (F3). Furthermore, the acceptance of taste, colour, aroma, texture, overall, and antioxidant activity level of shredded banana blossom-chicken meat. Results: There was a significant difference in the acceptance of the colours of shredded F1, F2 and F3 (p = 0,00), while aroma, taste, texture and overall of the shredded F1, F2 and F3 were not significant (p> 0.05). Shredded F3 (banana blossom 25%: chicken meat 75%) is most preferred based on the parameters of colour, aroma, taste, texture and overall, and has the highest antioxidant activity value are 53.74%.Conclusions: Shredded banana blossom-chicken meat has a good acceptability and has a high antioxidant activity level.[AN1]  

scholarly journals Antioxidant Activity of Sunflower (Helianthus Annuus l.) Ethanolic Extract with DPPH Method and Determination of Total Phenolic and Flavonoid Levels

2021 ◽  
Vol 4 (1) ◽  
pp. 31-42
Author(s):  
Salwa Salwa
Keyword(s):  
Antioxidant Activity ◽  
Helianthus Annuus ◽  
Antioxidant Properties ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Helianthus Annuus L ◽  
A Value ◽  
Dpph Method

Sunflower (Helianthus annuus L.) is believed to have antioxidant properties. Currently, there are many studies examining compounds that have benefits as antioxidants and are safe for health. However, the sunflower only examined the antioxidants in sunflower seeds. This study aims to determine the antioxidant activity of ethanol extract of flowers, bark, and leaves of sunflowers. The antioxidant activity test using the DPPH method showed the highest antioxidant activity was found in the leaf extract (Helianthus annuus L.) with an IC50 value of 48.841 ppm. The highest phenolic content was found in leaf samples with a value of 35.149 mg gallic acid equivalent/gram extract and the highest determination of flavonoid levels was found in leaf samples with a value of 10.917 mg quercetin equivalent/gram extract.

Phytochemical Test, Determination of Total Phenol, Total Flavonoids and Antioxidant Activity of Ethanol Extract of Moringa Leaves (Moringa oleifera Lam)

2020 ◽  
Vol 23 (8) ◽  
pp. 290-294
Author(s):  
Enny Fachriyah ◽  
Dewi Kusrini ◽  
Ifan Bagus Haryanto ◽  
Synta Mutiara Bunga Wulandari ◽  
Widyaningrum Islami Lestari ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Moringa Oleifera ◽  
Ethanol Extract ◽  
Total Phenol ◽  
Total Flavonoids ◽  
Test Determination ◽  
Moringa Oleifera Lam

UJI AKTIVITAS ANTIOKSIDAN FRAKSI N-HEKSAN DAUN AFRIKA (Vernonia amygdalina Del.) DENGAN METODE DPPH

2020 ◽  
Vol 5 (2) ◽  
pp. 250-257
Author(s):  
Nurul Fatimah ◽  
◽  
Reksi Sundu
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Free Radicals ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Hexane Fraction ◽  
Phytochemical Screening ◽  
Vernonia Amygdalina ◽  
Tropical Africa ◽  
Ic50 Value

Free radicals and reactive species are widely believed to contribute to the development of several diseases by causing oxidative stress and eventually oxidative. Vernonia amygdalina (Astereacea) is a small shrub or tree between 1 and 5m high growing throughout tropical Africa. Plants are generally known as bitter leaves is well cultivated and is a general market for merchandise in several countries. The purpose of this study was to determine the antioxidant activity of hexane fraction from ethanol extract od Frican leaves (Vernonia amygdalina Del.). The method used in this study was the DPPH (1,1-Diphenil-2-Picrylhydrazyl) method. The result of phytochemical screening showed that ethanolic extract of African leaves contained a composition of secondary metabolites of alkaloids, flavonoids, tannins, steroids/triterpenoids and saponins. The antioxidant activity of the extract of n-hexane fraction was classified as very weak with an IC50 value of 317.98 ppm.

UJI IMUNOSTIMULATOR DARI KOMBINASI EKSTRAK ETANOL HERBA BINARA (ARTEMISIA VULGARIS LINN) DAN EKSTRAK ETANOL DAUN PUCUK MERAH (SYZYGIUM OLEANA) DENGAN METODE HIPERSENSITIVITAS TIPE LAMBAT PADA TIKUS JANTAN

2019 ◽  
Vol 1 (2) ◽  
pp. 22-26
Author(s):  
Romauli Anna Teresia Marbun ◽  
Aminah Syarifuddin ◽  
Montysory Silalahi ◽  
Radika Bella Fista Ginting
Keyword(s):  
Antioxidant Activity ◽  
Immune System ◽  
Secondary Metabolites ◽  
Ethanol Extract ◽  
Positive Control ◽  
Artemisia Vulgaris ◽  
Chemical Screening ◽  
Chemical Content ◽  
High Antioxidant Activity ◽  
New Treatments

Diseases mediated by the immune system are difficult problems to treat such as human immunodeficiency virus (HIV) and other lethal viruses. Infections that occur in normal people are generally brief and rarely leave permanent damage. Treatment of this disease requires an aggressive and innovative approach to the development of new treatments so that it requires the role of immunomodulators to improve the immune system. A substance that acts as an enhancer or immune enhancer can be obtained by using herbs that are efficacious as immunostimulants. One of the herbs used is herbal binara (Artemisia vulgaris L) which has been studied as a potential immunomodulator with high antioxidant activity. Previous research also stated that red shoots (Syzygium oleana) were studied as potential immunomodulators with high antioxidant activity. Several other species such as Syzygium samarangense have 16 flavonoida compounds which show pharmacological immunological activity. The purpose of this study was to determine the content of secondary metabolites of ethanol extract of herbal binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) and to determine the best dose of extract from the ethanol extract of herbal binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) can reduce the volume of swelling of mouse feet. Examination of the chemical content of secondary metabolites from the ethanol extract of herbal binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) is carried out by chemical screening and characterization of simplicia and extract. The method used is the slow type hypersensitivity method. In this test the independent variable is the secondary metabolite of ethanol extract of herb binara (Artemisia vulgaris L.) with red shoots (Syzygium oleana) with four concentrations (50, 100, 200 and 400 mg / kgBB). The positive control used by Stimuno dose is 32.5 mg / kgBB

scholarly journals UJI AKTIVITAS ANTIOKSIDAN SPONS Stylissa sp. DENGAN MENGGUNAKAN METODE DPPH (1,1-difenil-2-pikrilhidrazil)

PHARMACON ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 419
Author(s):  
Samuel I.M. Sibarani ◽  
Adithya Yudistira ◽  
Deby A. Mpila
Keyword(s):  
Antioxidant Activity ◽  
Cell Wall ◽  
Free Radicals ◽  
Ethanol Extract ◽  
Dna Bases ◽  
Oxidation Reactions ◽  
Terrestrial Plants ◽  
Dpph Method ◽  
High Antioxidant Activity ◽  
Ethanol Extracts

ABSTRACTHabitat of sponge Stylissa sp., were under the sea and these sponges contain active compounds, which are more active than the compounds produced by terrestrial plants. Antioxidants are inhibitors of oxidation reactions due to the free radicals, which can cause weak damage to unsaturated cells, cell wall membranes, blood vessels, DNA bases, and lipid tissue, that causing disease. The study was conducted to determine the antioxidant activity of ethanol extracts  of sponge Stylissa sp., which is located on the Bangka Island, Likupang District, North Minahasa Regency. Sponge Stylissa sp., was extracted by maceration with using ethanol. Testing of antioxidant activity was carried out by the DPPH method measured by a UV-Vis spectrophotometer. The results of the study showed that ethanol extracts of sponge Stylissa sp., has antioxidant activity in each concentration and the highest at a concentration of 100 mg / L. The conclusion is a ethanol extract of sponge Stylissa sp. have high antioxidant activity with a concentration of 25 mg/L (77,40%), concentration of 50 mg/L (85,63%), concentration of 75 mg/L (88,66%), and concentration 100 mg/L (88,96%). Key words: Stylissa sp. Sponge, Antioxidant, DPPH (1,1-diphenyl-2-picrylhydrazyl) ABSTRAKHabitat dari spons Stylissa sp. terdapat di bawah laut dan spons ini mengandung senyawa aktif yang persentase keaktifannya lebih besar dibandingkan dengan senyawa-senyawa yang dihasilkan oleh tumbuhan darat. Antioksidan adalah zat penghambat reaksi oksidasi akibat radikal bebas yang dapat menyebabkan kerusakan lemah tak jenuh, membran dinding sel, pembuluh darah, basa DNA, dan jaringan lipid sehingga menimbulkan penyakit. Penelitian dilakukan untuk mengetahui aktivitas antioksidan ekstrak etanol biota laut spons Stylissa sp. yang terdapat di pulau Bangka, Kecamatan Likupang, Kabupaten Minahasa Utara. Spons Stylissa sp. ini diekstrak dengan metode maserasi menggunakan etanol. Pengujian aktivitas antioksidan ini dilakukan dengan metode DPPH yang diukur dengan alat spektrofotometer UV-Vis. Hasil dari penelitian menunjukan bahwa ekstrak etanol spons Stylissa sp. memiliki aktivitas antioksidan disetiap konsentrasi dan yang tertinggi pada konsentrasi 100 mg/L. Kesimpulan yang didapat bahwa ekstrak etanol spons Stylissa sp. memiliki aktivitas antioksidan yang tinggi dengan konsentrasi 25 mg/L (77,40%), konsentrasi 50 mg/L (85,63%), konsentrasi 75 mg/L (88,66%), dan konsentrasi 100 mg/L (88,96%). Kata Kunci : Spons Stylissa sp., Antioksidan, DPPH (1,1-difenil-2-pikrilhidrazil)

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