scholarly journals Total Phenol and Antioxidant Activity of Ethanol Extract and Water Extract from Claw Uncariaa gambir Roxb

2021 ◽  
Vol 9 (3) ◽  
pp. 131
Author(s):  
Lucky Hartanti ◽  
Asri Mulya Ashari ◽  
Warsidah Warsidah
Keyword(s):  
Antioxidant Activity ◽  
Aqueous Extract ◽  
Water Extract ◽  
Ethanol Extract ◽  
Total Phenol ◽  
Total Phenolic ◽  
Market Demand ◽  
University Campus ◽  
Industrial Plants

Uncaria gambier Roxb is a plant from the Rubiaceae family, belongs to the Bajakah group and has been used empirically in the treatment of various types of diseases in the community. Besides being one of the industrial plants and export commodities produced in Sumatra and Kalimantan, gambier leaves is also used frequently in Indonesia, both for the pharmaceutical and cosmetic industries. As a result, market demand both nationally and internationally have increased. The aim of this research are determination of antioxidant activity of ethanol extract and aqueous extract of gambir claw using 1,1-diphenil-2-picrylhydrazyl (DPPH) and determination of total phenol using Calcetau folin reagent. Gambier plant samples were taken in the forest area around the Tanjungpura University campus, the claws were separated and dried at room temperature, then powdered and extracted by maceration using 70% ethanol pa and aquadest, then the extract was concentrated until it was ready to be tested for antioxidant activity and determination of total phenol. The total phenolic ethanol extract and aqueous extract of gambir claw were 224.66 mg GAE/g extract (GEA : Gallic Acid Eqivalent) and 299.08 mg GAE/g extract, respectively, while the antioxidant potential (IC50) was 39.566 μg/mL and 65.140 μg/mL, and the IC50 for comparison of Vitamin C was 7.02 μg/mL.

scholarly journals Chromatographic Analysis (LC-MS and GC-MS), Antioxidant Activity, Total Phenol and Total Flavonoid Determination of Ononis natrix L. Grown in Jordan

2021 ◽  
Vol 16 (1) ◽  
pp. 31-39
Keyword(s):  
Solid Phase ◽  
Water Extract ◽  
Ethanol Extract ◽  
Total Phenol ◽  
Aroma Profile ◽  
Sesquiterpene Hydrocarbons ◽  
Leaf Oil ◽  
Terpenoid Aldehydes ◽  
Ethanol Extracts

The purpose of the present study was to identify, by gas chromatography (GC) and GC-mass spectrometry (GC-MS), the components of the spontaneously emitted volatile organic compounds (VOCs), obtained by Solid-Phase-Micro-Extraction (SPME) and that of the hydrodistilled oil of the fresh flowers and leaves of Ononis natrix, as well as to compare them. The hydrodistilled leaf oil was rich in non-terpenoid aldehydes, whereas its aroma profile contained mainly sesquiterpene hydrocarbons with α-copaene and germacrene D as their major components. The hydrodistilled oil of the fresh flowers, however, revealed nearly equal amounts of terpenoid and non-terpenoid compounds; 51.00% and 46.54%, respectively. The aroma profile of the fresh flowers was dominated by monoterpene hydrocarbons with α-pinene (42.96%) and α-thujene (20.17%) as the predomi¬nant two monoterpenes. Based on the high total phenol and flavonoid contents of the water and ethanol extracts, LC-MS analysis was carried out to identify the major compounds from each sample. From the water extract, eleven compounds were identified, whereas the ethanol extract contained eight, whereby luteolin (from the water extract) and apigenin (from the ethanol one) were named as the major flavonoids, respectively.

scholarly journals Comparative Study on Phenolic Compounds and Antioxidant Activities of Hop (Humulus lupulus L.) Strobile Extracts

Plants ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 135
Author(s):  
Jae Il Lyu ◽  
Jaihyunk Ryu ◽  
Kyoung-Sun Seo ◽  
Kyung-Yun Kang ◽  
Sang Hoon Park ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Phenolic Compound ◽  
Antioxidant Activities ◽  
Water Extract ◽  
Ethanol Extract ◽  
Antioxidant Property ◽  
Flavonoid Compound ◽  
Total Phenolic ◽  
Extraction Solvent

In this study, we investigated the phenolic compounds in hop strobile extracts and evaluated their antioxidant property using DPPH and ABTS assay. The total phenolic compound (TPC) and total flavonoid compound (TFC) estimated in two different solvent extracts considerably varied depending on the extraction solvent. The most abundant phenolic compound in hop strobile was humulones (α-acid) with levels ranging from 50.44 to 193.25 µg/g. El Dorado accession revealed higher antioxidant activity in ethanol extracts (DPPH: IC50 124.3 µg/mL; ABTS: IC50 95.4 µg/mL) when compared with that of the other accessions. Correlations between DPPH (IC50) scavenging TFC in ethanol extract (TFC_E, −0.941), and TPC_E (−0.901), and between ABTS (IC50) scavenging TFC_E (−0.853), and TPC_E (−0.826), were statistically significant at p < 0.01 level, whereas no significant correlation was observed between antioxidant activities, TPC and TFC in water extract. This study is the first to report that variations in the level of phenolic contents and antioxidant activity of various hop cultivars depended on the type of extraction solvent used and the cultivation regions. These results could provide valuable information on developing hop products.

scholarly journals Determination of phenolic contents and antioxidant activity test of ethanol extract of Sirih merah (Piper crocatum Ruiz & Pav.) leaves using the DPPH method

2021 ◽  
Vol 13 (2) ◽  
pp. 137-142
Author(s):  
Kasta Gurning ◽  
◽  
Sifikal Lumbangaol ◽  
Risanti F. R. Situmorang ◽  
Saronom Silaban ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Extraction Process ◽  
Total Phenolic ◽  
Betel Leaf ◽  
Dpph Method

The research objectives were to identify the secondary metabolite components, total phenolic content and determine the antioxidant activity of the ethanol extract of red betel leaf (Piper crocatum Ruiz & Pav.). The extraction process was carried out by materation using ethanol as a solvent. Determination of total phenolic content was carried out colorimetrically with Folin-Ciocalteu reagent measured at a maximum wavelength of 765 nm. Determination of antioxidant activity using the DPPH method measured by spectrophotometry at a maximum wavelength of 517 nm. The results of phytochemical screening of the ethanolic extract of red betel leaf contain secondary metabolites, including flavonoid, phenolic, tannin, alkaloids, steroids, and triterpenoids. The total phenolic content of the red betel leaf ethanol extract was 0.949±0.003 mg GAE/g d.w. and has antioxidant activity (IC50) 84,656 including strong category as an antioxidant. Keywords: Piper crocatum Ruiz & Pav., Antioxidant, Ethanol extract, Folin-Ciocalteu and DPPH

scholarly journals Antioxidant activity of Trachystemon orientalis (L.) G. Don (Borage) grown and eaten as food in Ordu, Turkey

2015 ◽  
Vol 61 (4) ◽  
pp. 40-51 ◽  
Author(s):  
Melek Col Ayvaz
Keyword(s):  
Antioxidant Activity ◽  
Natural Products ◽  
Total Antioxidant Capacity ◽  
Water Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Synthetic Antioxidants ◽  
Total Antioxidant ◽  
Phenolic And Flavonoid

SummaryIntroductıon: Instead of synthetic antioxidants, using of natural products with antioxidant activity is demanded.Objectıve: The aim of this study is to evaluate the antioxidant activity and total phenolic and flavonoid contents of theTrachystemon orientalis(borage) obtained from Ordu.Methods: The water and ethanol extracts of the borage plant were investigated by using severalin vitroantioxidant activity tests such as total antioxidant capacity, O2•−,•OH, DPPH and ABTS radicals scavenging activities, hydrogen peroxide scavenging activity, FRAP, Fe2+chelating and reducing power assays in order to make effective comparisons.Results: Water extract of theT. orientalishad the more phenolic (90 mg GA/g extract) and flavonoid (56.88 mg CT/g extract) contents than ethanol extract. Furthermore, both of the extracts showed strong antioxidant and radical scavenging activities determined by differentin vitromodels. The highest total antioxidant capacity as ascorbic acid equivalents of 244.50 mg/g extract was also calculated for water extract. Generally, a relationship between total phenolic content and antioxidant activity was established. It is also an important finding for an edible food source that the phenolic and flavonoid content and antioxidative activities for water extract was higher than the ethanol extract.Conclusıon:T. orientaliscould be used as a food ingredient instead of synthetic antioxidants and all results will contribute to the recent increase in investigations on using natural products in many areas such as food, pharmacy, alternative medicine and natural therapy.

scholarly journals Phytochemical Composition, Antioxidant Activity, and Neuroprotective Effect ofTerminalia chebulaRetzius Extracts

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Chia Lin Chang ◽  
Che San Lin
Keyword(s):  
Antioxidant Activity ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Neuroprotective Effect ◽  
Water Extract ◽  
Ethanol Extract ◽  
Total Phenolic ◽  
Cupric Sulfate ◽  
Neuroprotective Effects

The objectives of this study were to determine phytochemical compositions, chemiluminescence antioxidant activities, and neuroprotective effects on PC12 cells for water, methanol, and 95% ethanol extracts of the air-dried fruit ofTerminalia chebulaRetzius. The water extract afforded the greatest yield, and total phenolic and tannin content. The methanol extract yielded the greatest total triterpenoid content. Based on four chemiluminescence antioxidant assays, the three extracts showed various degrees of antioxidant activity. The methanol extract showed good antioxidant activity based on the horseradish peroxidase-luminol-hydrogen peroxide (H2O2) assay. The water extract appeared to have good antioxidant activities in cupric sulfate-Phen-Vc-H2O2and luminol-H2O2assays. Pyrogallol-luminol assay showed the 95% ethanol extract to have good antioxidant activity. The methanol and water extracts presented neuroprotective activities on H2O2-induced PC12 cell death at 0.5–5.0 μg/mL. Further investigations are necessary to verify these activitiesin vivo.

scholarly journals Extraction, Evaluation, and Antioxidant Activity of Total Phenol from Callus of Abutilon indicum (L.) Sweet

2021 ◽  
Vol 11 (3) ◽  
pp. 3652-3660
Keyword(s):  
Antioxidant Activity ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Water Extract ◽  
Hot Water ◽  
Total Phenol ◽  
Total Phenolic ◽  
Dpph Assay ◽  
Layer Chromatography ◽  
Abutilon Indicum

In this present study, callus induction from the leaves of Abutilon indicum was performed and evaluated antioxidant potential after TLC, PTLC. For induction of callus, Murashige, and Skoog (MS) medium with 2.5 mg/L concentration of 2, 4 Dichloro phenoxy acetic acid (2,4-D) and for assay, Folin Ciocalteau method practiced for evaluation of total phenol, modified spectrophotometer method used for DPPH assay. Based on total phenolic content, total antioxidant capacity, DPPH, assay, and reducing power assay performed after separation and partial purification of total phenols by Thin-layer chromatography (TLC) and Preparative thin-layer chromatography (PTLC), respectively. For total phenol estimation, hot water extract shows the highest total phenol content (10.56 μg/ml) by comparing another extract; similarly, PTLC fraction of callus extract from Abutilon indicum shows 61% of inhibition in DPPH at 10 μg/ml. From this study, compared to leaf, callus shows a higher concentration of total phenol and shows promising antioxidant activity. So, it gives scope for further exploration and purification and application of total phenol from callus of Abutilon indicum.

scholarly journals Determination of Total Phenolic, Total Flavonoid and Antioxidant Activity of Karamunting Leaves (Rhodomyrtus tomentosa (Aiton) Hassk) Extract and Fraction

2021 ◽  
Vol 9 (2) ◽  
pp. 72-78
Author(s):  
Rahma Dona
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethanol Extract ◽  
Ethyl Acetate Fraction ◽  
Total Flavonoid ◽  
Hexane Fraction ◽  
Total Phenolic ◽  
Water Fraction ◽  
Ic50 Value

Karamunting (Rhodomyrtus tomentosa (Aiton) Hassk) is a family of Myrtaceae which has been used by the community as traditional medicine. The purpose of this research is determine total phenolic and total flavonoid level and test antioxidant activity of Karamunting Leaves. Determination of total phenolic using gallic acid standard by the Folin Ciocalteu method. The results obtained total phenolic in water fraction of 99 mgGAE/g, ethanol extract 94,1 mgGAE/g, ethyl acetate fraction 83,3 mgGAE/g and n-hexane fraction 41,4 mgGAE/g. Determination of total flavonoid using standard quersetin with AlCL3 complex formation method, the results obtained total flavonoid in water fraction of 156,8 mgQE/g, ethanol extract 192,6 mgQE/g, ethyl acetate fraction 89,4 mgQE/g and n-hexane fraction 31,3 mgQE/g. Determination of antioxidant activity was determined by DPPH radical capture test (1,1-diphenyl-2-picrylhydrazyl), the results obtained in water fraction with IC50 value 15,02 µg/mL, ethanol extract 14,06 µg/mL, ethyl acetate fraction 14,48 µg/mL with very strong activity of antioxidant, whereas in the n-hexane fraction was categorized as weak antioxidant activity with an IC50 value of 264,02 µg/mL.

scholarly journals DETERMINATION OF QUALITY PARAMETERS AND TEST ANTIOXIDANT ACTIVITIES OF 70% ETHANOL EXTRACT OF SEROJA LEAVES (NELUMBO NUCIFERA GAERTN.)

2021 ◽  
pp. 30-33
Author(s):  
RATNA DJAMIL ◽  
DIAH KARTIKA PRATAMI ◽  
FEBI AYU PUTRI ◽  
THALIA BREBA OCTAVIA
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Quality Parameters ◽  
Medicinal Herb ◽  
Nelumbo Nucifera ◽  
Total Phenol ◽  
Antioxidant Power

Objective: The purpose of this study was to evaluated the quality parameters and analyzed the antioxidant activity of seroja leaves Nelumbo nucifera Gaertn. Methods: The quantification of the chemical compound was determined by its total phenol and flavonoid levels. The evaluate the antioxidant activity was determined by the comparability of the four common radical scavenging assays using 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS); 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical; cupric ion reducing antioxidant capacity (CUPRAC); ferric reducing antioxidant power (FRAP); and 2-thiobarbituric acid (TBA) methods. Results: The results of phytochemical screening for simplicia powder and 70% ethanol extract of seroja leaves contain secondary metabolites of alkaloids, flavonoids, saponins, tannins, coumarin, quinones, and triterpenoid steroids. The results of the determination of the quality parameters meet the requirements of quality and safety standard of the medicinal herb. The result of the determination of total phenol content from 70% ethanol extract of Seroja leaves was 181.62±0.82 mg GAE/g extract. The results of the determination of total flavonoid levels from 70% ethanol extract of seroja leaves amounted to 289.83±1.04 mg QE/g extract. The results of antioxidant activity tests using the ABTS, DPPH, and TBA methods showed IC50 respectively 287.7 mg/l, 22.3 mg/l, and 352.6 mg/l and CUPRAC and FRAP methods had an antioxidant capacity of 160.76±0.35 and 253.36±0.48 mg AAE/g extract. Conclusion: Seroja leaves (Nelumbo nucifera Gaertn.) have the potential to be used as an antioxidant medicinal herb and its extract meet the standard of quality control and safety.

scholarly journals PENENTUAN KADAR FENOLAT TOTAL DAN AKTIVITAS ANTIOKSIDAN DARI EKSTRAK DAUN DADAP MERAH (Erythrina fusca Lour) SECARA SPEKTROFOTOMETRI UV-Vis

2019 ◽  
Vol 11 (1) ◽  
pp. 09-16
Author(s):  
Epi Supri Wardi ◽  
Zulkarni R Zulkarni R ◽  
Desy Nurdianti
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Phenolic Content ◽  
Ethyl Acetate Extract ◽  
Ethanol Extract ◽  
Hexane Extract ◽  
Total Phenolic ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power

Determination of total phenolate and antioxidant activity of red leaf extract (Erythrina fusca Lour) was done by UV-Vis spectrophotometry. This study aims to determine the total phenolic content and antioxidant activity of hexane, ethyl acetate and ethanol extract. The extracts were prepared using a non-polar-maseration method with hexane, ethyl acetate and ethanol solvents. The results showed total phenolic concentration using the Folin-Ciocalteu method were 0.412 g/100 g in the hexane extract, 1.782 g/100 g in the ethyl acetate extract and 5.455 g/100 g in the ethanol extract. Antioxidant activity conducted by using FRAP method (Ferric Reducing Antioxidant Power) were obtained 0,682 mmol Fe (II)/100 g at hexane extract, 5,186 mmol Fe (II)/100 g at ethyl acetate extract and 10,591 mmol Fe (II)/100 g on the ethanol extract. The antioxidant activity of gallic acid as standard was 44.356 g mmol Fe (II)/100g.

scholarly journals Penentuan Total Fenolik, Uji Antioksidan, Dan Uji Antibakteri Pada Ekstrak Etanol Jantung Pisang Ambon (Musa acuminate Colla)

2021 ◽  
Vol 1 ◽  
pp. 1903-1914
Author(s):  
Ratna Tri Lestari ◽  
S Slamet ◽  
W Wirasti ◽  
Urmatul Waznah
Keyword(s):  
Antioxidant Activity ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Total Phenol ◽  
Antioxidant Compounds ◽  
Antimicrobial Function ◽  
High Antioxidant Activity ◽  
Banana Flower ◽  
Paper Disc

AbstractBanana plant is one type of plant that contains chemical compounds that can be used for treatment. One part of the plant that has not been fully utilized is the heart of the Ambon banana (Musa acuminate Colla). Banana flower is known to contain flavonoid compounds, phenols, alkaloids, tannins, saponins, and coumarins. One of the compounds belonging to the flavonoid group which generally includes natural antioxidant compounds and has an antimicrobial function is phenolic compounds. The purpose of this study was to determine the total phenols, to examine the activity of antioxidants, antibacterials, and the ethanol extract of Ambon banana flower (Musa acuminate Colla). Determination of total phenol using the GAE (Gallic Acid Equivalence) method, antioxidant analysis using the FRAP method, and the antibacterial activity test using the paper disc method. The total phenol yield obtained was 17.7291 ± 0.102 mg GAE/g extract, the results from the antioxidant test were obtained with an EC50 value of 0.0628 µmolFe2+/g sampel extract, which means that the ethanolic extract of Ambon banana flower has a high antioxidant activity value, and antibacterial results in the extract. with a medium average (5-10 mm). From the results of tests carried out, the ethanol extract of Ambon banana flower has antioxidant activity and has little antibacterial power.Keywords : Banana plants; Ambon banana flower; antioxidants; antibacterial; total phenol AbstrakTanaman pisang merupakan salah satu jenis tanaman yang mengandung senyawa kimia yang dapat digunakan untuk pengobatan. Salah satu bagian tanaman yang belum dimanfaatkan secara maksimal adalah jantung pisang ambon (Musa acuminate Colla). Jantung pisang diketahui mengandung senyawa flavonoid, fenol, alkaloid, tannin, saponin, dan kumarin. Salah satu senyawa yang termasuk golongan flavonoid yang umumnya termasuk senyawa antioksidan alami dan memiliki fungsi antimikroba yaitu senyawa fenolik. Tujuan dari penelitian ini yaitu penentuan total fenol, meneliti aktivitas antioksidan, antibakteri, dan pada ekstrak etanol jantung pisang ambon (Musa acuminate Colla). Penentuan total fenol menggunakan metode GAE (Equivalensi Asam Galat), analisis antioksidan menggunakan metode FRAP, dan uji aktivitas antibakteri menggunakan metode kertas cakram. Hasil total fenol yang diperoleh 17,7291 ± 0,102 mg GAE/g ekstrak, hasil dari uji antioksidan didapat dengan nilai EC50 pada ekstrak 0,0628 µmolFe2+/g sampel yang berarti ekstrak etanol jantung pisang ambon mempunyai nilai aktivitas antioksidan yang tinggi, dan hasil antibakteri pada ekstrak dengan rata-rata sedang (5-10 mm). Dari hasil pengujian yang dilakukan ekstrak etanol jantung pisang ambon mempunyai aktivitas antioksidan dan mempunyai sedikit daya antibakteri. Kata kunci : Tanaman pisang; jantung pisang ambon; antioksidan; antibakteri; total fenol.

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