scholarly journals Effect of bioagent formulations on progress of bacterial leaf blight disease of rice under field conditions

2013 ◽  
Vol 5 (2) ◽  
pp. 388-393
Author(s):  
Gokil Prasad Gangwar

In the present study, effectiveness of different fungal (Trichoderma harzianum) and bacterial (Pseudomonas fluorescens) bioagent formulations in reducing progress of the bacterial leaf blight disease of rice under field conditions was studied and compared with chemical treatment and untreated check. The results exhibited that after 23 to 30 days after first application, bioagent formulations were more effective then chemical treatment in reducing progress of disease. Bioagent formulations exhibited long lasting effect in reducing progress of disease during Kharif, 2006 and 2007. Application of bioagent formulations resulted in significant reduction (60.5 – 142.8%) in area under disease progress curve (AUDPC) as compared to check during Kharif, 2006 and 2007. Significant increase in grain yield (14.3 - 21.5 %) was observed with the application of bioagent formulations as compared to check during Kharif, 2006 and 2007.

2014 ◽  
Vol 6 (1) ◽  
pp. 234-238
Author(s):  
Gokil Prasad Gangwar ◽  
A. P. Sinha

The experiment was conducted to evaluate growth promotion of transplanted rice by fungal and bacterial bioagents effective against bacterial leaf blight of rice, under glasshouse conditions. Bioagent formulations (PBA -1, PBA -2, FLP 88, Pf 83, Isolate 40 and T. harzianum) were applied as seed treatment, seedling root dip and foliar spray and compared with chemical treatment and untreated check. All bioagent formulations were significantly effective in increasing number of tillers per hill, root length, total phenolic content in rice leaves, fresh and dry root weight, fresh and dry shoot weight, flag leaf area, grain yield and 1000 grain weight over check. Present study revealed that potential fungal and bacterial bioagents effective against bacterial leaf blight disease of rice, exhibited plant growth promoting activities and resulted 22.38 to 32.08 % increase in grain yield over untreated check, even if there is no disease.


2018 ◽  
Vol 152 (3) ◽  
pp. 747-757 ◽  
Author(s):  
Prashant P. Jambhulkar ◽  
Pratibha Sharma ◽  
Raja Manokaran ◽  
Dilip K. Lakshman ◽  
Pramod Rokadia ◽  
...  

2011 ◽  
Vol 11 (1) ◽  
pp. 35-46 ◽  
Author(s):  
Heru Adi Djatmiko ◽  
Budi Prakoso ◽  
Nur Prihatiningsih

One of the major diseases of rice paddy fields in Indonesia and the Asian countries is bacterial leaf blight or kresek caused by X. oryzae pv. oryzae (Xoo). Losses caused by the disease in Indonesia reached 70–80%, in India reached 74–81%, and Japan reached 20–50%, thus causing great losses in the economy. The objectives of that research were: 1) Characterize Xoo from Karesidenan Banyumas; 2) To study of the amount of damage and AUDPC (the area under disease progress curve) of bacterial leaf blight disease at Karesidenan Banyumas; 3) To obtain of Xoo pathotype by using the test varieties; 4) To obtaining genetic diversity of Xoo that found in Banjarnegara, Purbalingga, Banyumas, Cilacap dan Kebumen region. Research was carried out in several stages: isolation and characterization of Xoo from Barlingmascakeb region, testing of Xoo with five varieties testing, assesment of disease intensity of bacterial leaf blight and AUDPC in the field, and testing the genetic diversity of Xoo. The results showed that pathogen of bacterial blight on rice is Xoo characterized yellow colour of colonies on SPA medium, negative gram reaction, catalase positive, oxidase negative, negative growth at 0.1% TZC, negative starch hydrolisis , and resistance to 0.001% Cu(NO3)2 positive. Xoo pathotype isolats found in Banjarnegara was pathotype X, Cilacap were pathotype I and II and Purbalingga was pathotype II. Eighteen of Xoo from Karesidenan Banyumas (Banjarnegara, Purbalingga, Banyumas, Cilacap and Kebumen) of RAPD differ one from the others.


Antibiotics ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 517
Author(s):  
Nguyen Thi Thu Nga ◽  
Tran Ngoc Tran ◽  
Dominique Holtappels ◽  
Nguyen Le Kim Kim Ngan ◽  
Nguyen Phuoc Hao ◽  
...  

Bacterial leaf blight, which is caused by Xanthomonas axonopodis pv. allii, annually causes significant yield losses to Welsh onion in many producing countries, including Vietnam. In this study, we isolated and characterized lytic phages ɸ16, ɸ17A and ɸ31, specific to X. axonopodis pv. allii and belonging to a new phage species and genus within the Autographiviridae, from four provinces in the Mekong Delta of Vietnam. Moreover, we evaluated their efficacy for the biocontrol of leaf blight in greenhouse and field conditions. When applying the three highly related phages individually or as a three-phage cocktail at 108 PFU/mL in greenhouse conditions, our results show that treatment with ɸ31 alone provides higher disease prevention than the two other phages or the phage cocktail. Furthermore, we compared phage concentrations from 105 to 108 and showed optimal disease control at 107 and 108 PFU/mL. Finally, under field conditions, both phage ɸ31 alone and the phage cocktail treatments suppressed disease symptoms, which was comparable to the chemical bactericide oxolinic acid (Starner). Phage treatment also significantly improved yield, showing the potential of phage as a biocontrol strategy for managing leaf blight in Welsh onion.


2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Sherien M. M. Atalla ◽  
Mokhtar M. Abdel-Kader ◽  
Nadia G. El-Gamal ◽  
Nehal S. El-Mougy

Abstract Maize (Zea mays L.) is one of the most economic crops in Egypt. Production of amylase from the waste of maize is the most economic and cheap renewable and most abundant raw materials present in environment. Biosynthesis of Cu-chitosan nanoparticles for amylase production by co-culturing between Trichoderma harzianum and Pseudomonas fluorescens at different ratios compared to free conditions was the main purpose of this study. The optimum ratio 8:2, recorded between P. fluorescens: T. harzianum, was the most promising for production of amylase produce 22.47 and 28.60 U/ml for free and nano, respectively. The UV visible spectral analysis Cu-chitosan NPs was 220 nm, while the mean diameter, using transmission electron microscopy was 0.5 μm. Application of fermented maize wastes by co-cultivation of P. fluorescence and T. harzianum, as a grain dressing before sowing declared the reduction in both root and foliar diseases during the maize growing season, starting from germination up to 70 days of its vegetative growth under field conditions. A promising approach is the creation and use of environmentally safe products, whose protective effect is based on the induction of hydrolase inhibitors in plants.


Author(s):  
A. Khandual ◽  
M. K. Mishra ◽  
H. Swain ◽  
S. Mohanty ◽  
P. C. Rath ◽  
...  

2013 ◽  
Vol 5 (2) ◽  
pp. 423-426 ◽  
Author(s):  
Gokil Prasad Gangwar

In the present study, Trichoderma harzianum, T. hamatum, T. virens and three isolates of Trichoderma spp. were evaluated for their comparative efficacy against bacterial leaf blight of rice, under field conditions. All the bioagent formulations were significantly effective in reducing disease severity over check during Kharif season 2006 and 2007. T. harzianum was found to be most effective and resulted 48.26 and 59.22 % reduction in disease severity during Kharif season 2006 and 2007, respectively. Maximum increase in grain yield (16.21%) was recorded with T. harzianum followed by isolate 40 (14.58%), during Kharif season 2006. Whereas during Kharif season 2007, maximum increase in grain yield (18.95%) was recorded with T. harzianum which is followed by T. virens (14.65%) and isolate 40 (12.57 %). Present study revealed that T. harzianum (isolated from rice phylloplane) was found to be most effective in reducing disease severity and increasing grain yield as compared to other isolates obtained from different sources.


2018 ◽  
Vol 45 (12) ◽  
pp. 1251 ◽  
Author(s):  
Shasmita ◽  
Harekrushna Swain ◽  
Anuprita Ray ◽  
Pradipta K. Mohapatra ◽  
Ramani K. Sarkar ◽  
...  

Bacterial leaf blight (BLB) is a serious threat for rice (Oryza sativa L.) cultivation caused by the bacterial pathogen Xanthomonas oryzae pv. oryzae. The pathogen mainly damages the leaf chlorophyllous tissue, resulting in poor photosynthesis and causing up to 50% reductions in grain yield. In the present work, we have compared the structural and functional ability of the chloroplast of three varieties of rice with different degrees of susceptibility (TN1, highly susceptible; IR-20, moderately resistant; DV-85, resistant to BLB) treated with riboflavin (1 and 2 mM) and infected with BLB, with chlorophyll fluorescence as a tool. As indicated by the chlorophyll fluorescence technique, the disease progress curve and yield data, riboflavin acted as an effective vitamin for inducing resistance against BLB. Plants treated with riboflavin showed improved PSII activity, more chlorophyll content and higher yield than the diseased plants.


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