scholarly journals Effect of paper industry effluent on enzyme activity and protein profiling of Chickpea (Cicer arietinum L.)

2017 ◽  
Vol 9 (4) ◽  
pp. 1910-1915
Author(s):  
Rajat Chaudhary ◽  
Anurag Mishra ◽  
Kapil Kumar ◽  
Sonam Arya
Keyword(s):  
Cicer Arietinum ◽  
Tap Water ◽  
Protein Profile ◽  
Paper Industry ◽  
Polyacrylamide Gel Electrophoresis ◽  
Industrial Effluent ◽  
Maximum Activity ◽  
Protein Profiling ◽  
Cicer Arietinum L ◽  
Industry Effluent

Chickpea (Cicer arietinum L.) is a legume which is mostly cultivated in India than other countries that can give significant amounts of dietary minerals and protein to humans. The effect of paper industrial effluent on chick-pea (C. arietinum L.) were analysed along with different concentration (10%, 20% 40%, 60% 80% and 100%) and pure tap water as a control to compare the effect of paper industrial effluent for one week. The amount of protein were comparable with control, their amount was increased at 40% in effluent treated seeds. The maximum activity of enzymes was found below 40% level of effluent. In this study protein profile of imbibed seeds, shoot, root and residual cotyledons were examined under the different concentration of effluent. Polyacrylamide gel electrophoresis of total protein showed that the maximum number of protein bands seen in the imbibed seeds whereas minimum number of protein bands observed in the root. SDS-PAGE revealed that less degradation and/or more rapid accumulation of proteins occurred in higher molecular weight proteins. From this study, it is clear that the industrial effluent rich in organic matter and plant nutrients are finding their use in agriculture as the cheaper way of disposal.

scholarly journals Protein profiling of chickpea (Cicer arietinum L.) during wilt disease (Fusarium oxysporum f. sp. ciceri)

2020 ◽  
Vol 8 (3) ◽  
pp. 2896-2901
Author(s):  
Jasminkumar Kheni ◽  
Rukam Singh Tomar ◽  
Mita Shihora ◽  
Shital Padhiyar ◽  
Hiralben Desai ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Cicer Arietinum ◽  
Wilt Disease ◽  
Protein Profiling ◽  
Cicer Arietinum L

scholarly journals Morphological and Biochemical Analysis of Cicer arietinum L. under Paper Industrial Effluent Stress conditions

2016 ◽  
Vol 8 (4) ◽  
pp. 2099-2103
Author(s):  
Rajat Chaudhary ◽  
Sonam Arya ◽  
Shweta Tyagi ◽  
Anurag Mishra ◽  
Vaishali Vaishali
Keyword(s):  
Organic Matter ◽  
Chlorophyll Content ◽  
Cicer Arietinum ◽  
Biochemical Analysis ◽  
Industrial Effluent ◽  
Reducing Sugar ◽  
Total Hardness ◽  
Chemical Characteristics ◽  
Cicer Arietinum L ◽  
Physico Chemical

To study the effect of paper industrial effluent on chickpea (Cicer arietinum L.) along with different concentration (10%, 20% 40%, 60% 80% and 100%) and pure tape water as a control to compare the effect of paper industrial effluent for 7 days. Physico-chemical characteristics of paper effluent were analyzed in terms of pH, colour, order temperature, DO, BOD, COD, Total hardness, carbonated hardness. All the parameters were found to be higher than the WHO prescribed discharge limits for effluent. The amount of carbohydrate, protein and reducing sugar were comparable with control, their amount were increased at 40% in effluent treated seeds. The chlorophyll content was increased simultaneously with effluent concentration. From this study it is clear that the industrial effluent rich in organic matter and plant nutrients are finding their use in agriculture as cheaper way of disposal.

scholarly journals Experimental and Techno-Economic Study on the Use of Microalgae for Paper Industry Effluents Remediation

2021 ◽  
Vol 13 (3) ◽  
pp. 1314
Author(s):  
Maria I. Silva ◽  
Ana L. Gonçalves ◽  
Vítor J. P. Vilar ◽  
José C. M. Pires
Keyword(s):  
Urban Areas ◽  
Net Present Value ◽  
Paper Industry ◽  
Industrial Effluent ◽  
Environmental Benefits ◽  
Co2 Uptake ◽  
Economic Study ◽  
Bioenergy Production ◽  
Legal Limits ◽  
Industry Effluent

Humanity is facing some major global threats, namely lack of environmental sustainability, the energy crisis associated with the unsustainable reliance on fossil fuels, and water scarcity, which will be exacerbated with the rapid growth of urban areas. Researchers have drawn their attention to microalgae, photosynthetic microorganisms known for their environmental applications, such as wastewater remediation and lipids accumulation, to produce third-generation biofuels to solve some of these major issues. Considering this dual role, this study evaluated the potential of the microalga Chlorella vulgaris on nutrient removal from a paper industry effluent and bioenergy production. Firstly, experiments were performed to assess the potential of this microalga to: (i) successfully grow in different concentrations of a paper industry effluent (20% to 100%); and (ii) treat the industrial effluent, reducing phosphorus concentrations to values below the accepted legal limits. Then, a techno-economic assessment was performed to study the viability of a C. vulgaris biorefinery targeting the remediation of a paper industry effluent and bioenergy production. The results have shown that C. vulgaris was able to successfully grow and treat the paper industry effluent. Under these conditions, average biomass productivities determined for this microalga ranged between 15.5 ± 0.5 and 26 ± 1 mg dry weight (DW) L−1 d−1, with maximum biomass concentrations reaching values between 337 ± 9 and 495 ± 25 mg DW L−1 d−1. Moreover, final phosphorus concentrations ranged between 0.12 ± 0.01 and 0.5 ± 0.3 mg P L−1, values below the legal limits imposed by the Portuguese Environment Agency on the paper industry. Regarding the proposal of a microalgal biorefinery for the bioremediation of paper industry effluents with bioenergy production, the techno-economic study demonstrated that six of the seven studied scenarios resulted in an economically-viable infrastructure. The highest net present value (15.4 million euros) and lowest discounted payback period (13 years) were determined for Scenario 3, which assumed a photosynthetic efficiency of 3%, a lipids extraction efficiency of 75%, and an anaerobic digestion efficiency of 45%. Therefore, it was possible to conclude that besides being economically viable, the proposed biorefinery presents several environmental benefits: (i) the remediation of an industrial effluent; (ii) CO2 uptake for microalgal growth, which contributes to a reduction in greenhouse gases emissions; (iii) production of clean and renewable energy; (iv) soil regeneration; and (v) promotion of a circular economy.

scholarly journals Altered EPS Protein Secretion in Salmonella Typhimurium Biofilm Following Treatment with DMSO

2019 ◽  
Vol 7 (4.14) ◽  
pp. 63
Author(s):  
M F Z Raja Yahya ◽  
Z Alias ◽  
S A Karsani
Keyword(s):  
Cell Surface ◽  
Surface Chemistry ◽  
Salmonella Typhimurium ◽  
Protein Secretion ◽  
Protein Profile ◽  
Polyacrylamide Gel Electrophoresis ◽  
Protein Band ◽  
Protein Profiling ◽  
Dmso Treatment ◽  
The Impact

Dimethyl sulfoxide (DMSO) is known to inhibit biofilm formation by Salmonella typhimurium and cause infrared spectral variations of extracellular polymeric substance (EPS) matrix. However, the impact of DMSO treatment on EPS protein secretion in S. typhimurium biofilm remains not well investigated. The present study was performed to investigate the EPS protein profile of S. typhimurium biofilm following treatment with DMSO. Biofilm was developed in 6-well microplate at 37 OC for 24 hours. Quantitative resazurin assay and infrared spectroscopy were performed to determine biofilm viability and cell surface chemistry respectively. Subtractive EPS protein profiling was carried out using a combination of polyacrylamide gel electrophoresis and tandem mass spectrometry. Treatment with 32% DMSO caused significant reduction in biofilm viability and alteration of cell surface chemistry. Subtractive comparison of EPS protein profiles highlighted one unique protein band, 52.1 kDa, which was secreted only in 32% DMSO treated EPS matrix and not in control EPS matrix. In turn, three EPS proteins were successfully identified from the 51.2 kDa protein band. In conclusion, altered EPS protein secretion following treatment with 32% DMSO may mediate inhibition of S. typhimurium biofilm.  

scholarly journals Enzyme Activities and Degradation of Nutrients in Chickpea (Cicer arietinum L.) Seeds during Germination

1970 ◽  
Vol 16 ◽  
pp. 29-34 ◽  
Author(s):  
MS Rahman ◽  
NK Sana ◽  
MM Hasan ◽  
ME Huque ◽  
RK Shaha
Keyword(s):  
Cicer Arietinum ◽  
Starch Content ◽  
Hydrolytic Enzymes ◽  
Seed Storage ◽  
Maximum Activity ◽  
Water Soluble ◽  
Free Sugar ◽  
Cicer Arietinum L ◽  
Water Soluble Protein ◽  
Carbohydrate Depletion

BARI-3 varieties of chickpea (Cicer arietinum L.) seed at different germinating periods. Among the varieties, the highest amylase activity was found in BARI-2 and lowest in BARI-3 during germination at 45 hours in water. The maximum activity of invertase was found in BARI-2 and minimum in BARI-1 at 72 hours of germination. Lipase activity was highest in BARI-2 and lowest in BARI-1. The highest protease activity was found in BARI-2 and lowest in BARI-3. The amount of total protein and water-soluble protein were found to be highest in BARI-1 and BARI-3, respectively. During light germination, carbohydrate depletion starts after initial imbibitions, and was completed in 120-144 hours. The variety BARI-1 was found to contain the highest amount of free sugar while BARI-3 had the lowest amount. BARI-2 was found to contain highest amount of reducing sugar and BARI-1 contained the lowest amount. The highest amount of starch was found in BARI-3 and lowest in BARI-1. The starch content in chickpea seed decreased gradually during germination. Among the varieties, BARI-1 was found to contain the highest amount of lipid while BARI-3 contained the lowest amount. The seed storage substances were found to decrease gradually with the increase of germination time. The results indicate that degradation of reserve seed nutrients accelerate the development of seedling growth during germination. Key words: Chickpea (Cicer arietinum L.) seed, Carbohydrate, Protein, Lipid, Hydrolytic Enzymes.   DOI:10.3329/jbs.v16i0.3738 J. bio-sci. 16: 29-34, 2008

DIFERENCIACIÓN DE ACCESIONES DE GARBANZO MEDIANTE CARACTERES MORFOLÓGICOS Y MOLECULARES

2018 ◽  
Vol 41 (4) ◽  
pp. 469-476
Author(s):  
Ernestina Valadez-Moctezuma ◽  
Anselmo de J. Cabrera-Hidalgo
Keyword(s):  
Cicer Arietinum ◽  
Cicer Arietinum L

El garbanzo (Cicer arietinum L.) es una de las principales leguminosas de grano cultivadas en el mundo. México es de los diez principales productores a nivel mundial con una producción total de 171 mil toneladas de grano. Este cultivo presenta alto nivel de autogamia y de monotonía genética, lo que dificulta la diferenciación de genotipos élite. En este estudio preliminar se evaluó la variabilidad de 57 genotipos de diferentes áreas geográficas mediante caracteres morfológicos y marcadores moleculares tipo RAPD e ISSR. El análisis de correspondencia múltiple mostró que los mayores valores discriminantes fueron el color de la flor azul y semillas grandes y de forma redondeada, pero el agrupamiento respectivo no diferenció a las accesiones, incluyendo las especies silvestres; sin embargo, el análisis UPGMA logró una mejor separación. Los marcadores RAPD aun cuando generaron perfiles de ADN, no fueron informativos, mientras que los ISSR diferenciaron a las 57 accesiones de C. arietinum utilizadas y a la especie silvestre C. reticulatum, lo que los hace buenos candidatos para caracterizar este cultivo. Este estudio sirvió como base para desarrollar otro sistema de marcadores moleculares más eficiente en esta especie.

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