THE ANXIOLYTIC ACTION OF NATURAL AND MICROPROPAGATED PLANT EXTRACTS OF BACOPA MONNIERI (L.) IN SWISS ALBINO MICE BY ELEVATED PLUS MAZE AND SWIM TEST

Background: The objective of this study was to evaluate the attenuation of anxiety on acute administration of aqueous extract of Terminalia belerica fruit pulp (AETBFP) by using elevated plus maze test and dark and light arena models.Methods: Thirty Swiss albino mice were divided into five groups, Group I received vehicle (1% Gum acacia suspension, 3ml/kg, orally), Group II received standard drug Diazepam (1mg/kg, orally) and Group III, IV and V received AETBFP 9, 18 and 36 mg/kg, orally respectively. In elevated plus maze test, the mouse was placed on the central platform facing towards open arm. The percentage of time spent and frequency of entries and number of rears in open arm was counted for a period of 5 min. In dark and light arena, the time spent, number of entries and number of rears in light arena was counted for a period of 5 min. The mean±SEM values were calculated for each group. The data was analyzed using one-way ANOVA followed by Dunnet’s multiple comparison tests; p< 0.05 was considered as statistically significant.Results: Significant (p<0.05) reduction in anxiety was noted in experimental animals when given at a dose of AETBFP (36mg/kg), where number of entries and duration of stay in open arm and light arena increased in elevated plus maze and light and dark arena respectively when compared with control animals.Conclusions: Our study reveals that AETBFP at a dose of 36mg/kg has significant attenuation of anxiety in Swiss albino mice.

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ANTIANXIETY EFFECT OF ETHANOLIC EXTRACT OF FRUITS OF LIMONIA ACIDISSIMA IN SWISS ALBINO MICE

International Journal of Pharmaceutical Sciences and Medicine ◽

10.47760/ijpsm.2021.v06i08.007 ◽

2021 ◽

Vol 6 (8) ◽

pp. 104-117

Author(s):

Chouhan Vaishali ◽

Sujane Sandhya ◽

Bapna Rajendra ◽

Pyathi Asharani

Keyword(s):

Elevated Plus Maze ◽

Treatment Options ◽

Ethanolic Extract ◽

Anxiolytic Effect ◽

Ethanol Extract ◽

Active Constituent ◽

Swiss Albino Mice ◽

Plus Maze ◽

Albino Mice ◽

Limonia Acidissima

Anxiety disorder is one of the most common mental ailments exhibited by human. It is characterized by motor tension, sympathetic hyperactivity and apprehension and vigilance syndromes. A part from very few chemical remedies available like benzodiazepine and serotonin modulators, not much treatment options are at hand that safely and effectively alleviate anxiety. The ethanol extract of the fruits of plant Limonia acidissima was evaluated for its anti-anxiety activity in swiss albino mice at dose 200mg/kg and 400mg/kg body weight. Anti-anxiety was assessed by using elevated plus maze (EPM) and light and dark model methods. The ethanolic extract exhibit anxiolytic effect in experimental mice. So it is supports the use of Limonia acidissima as anxiolytic agents. Further investigation should be made to elucidate the active constituent of responsible for the activity.

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The antianxiety activity of aqueous extract of Withania coagulans fruits in swiss albino mice by elevated plus maze

International Journal of Basic & Clinical Pharmacology ◽

10.18203/2319-2003.ijbcp20203617 ◽

2020 ◽

Vol 9 (9) ◽

pp. 1337

Author(s):

Devesh D. Gosavi ◽

Amit S. Kamdi ◽

Suvarna M. Kalambe ◽

Pankaj N. Bohra

Keyword(s):

Aqueous Extract ◽

Open Space ◽

Elevated Plus Maze ◽

P Value ◽

Swiss Albino Mice ◽

Elevated Plus Maze Test ◽

Maze Test ◽

Withania Coagulans ◽

Plus Maze ◽

Albino Mice

Background: Several anti-anxiety drugs used today have the numerous side effects. The Withania coagulans a rare species, is not studied much for its actions on anxiety except in late seventies where it was explored for neuropsychopharmacology. Thus, it was thought commendable to explore the anti-anxiety activities of aqueous extract of Withania coagulans fruits in swiss albino mice using elevated plus maze test.Methods: Elevated plus maze (EPM) test was used for evaluating the anti-anxiety action of Withania coagulans fruits’ aqueous extract. If the extract had anti-anxiety action, then it was expected that the time spent in the open space would increase and that in the closed space would decrease. This increase in time in the open space, if found statistically significant, was considered for antianxiety action.Results: There was statistically highly significant (p value <0.001) association observed between the aqueous extract of Withania coagulans fruits with antianxiety action in the swiss albino mice by elevated plus maze test.Conclusions: The aqueous extract of Withania coagulans fruits did prove the antianxiety effect in the swiss albino mice by elevated plus maze test.

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Evaluation of anti-anxiety activity of Mucuna pruriens

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v9i4-a.3420 ◽

2019 ◽

Vol 9 (4-A) ◽

pp. 104-107

Author(s):

Shobhit Singh ◽

Pushpraj Gupta ◽

Rishikesh Gupta

Keyword(s):

Elevated Plus Maze ◽

Standard Dose ◽

The Body ◽

Mucuna Pruriens ◽

Control Group ◽

Velvet Bean ◽

Swiss Albino Mice ◽

Plus Maze ◽

Albino Mice

Mucuna pruriens, also known as Velvet Bean, Mucuna pruriens has been used for centuries by Ayurvedic herbalists for overall wellness. Mucuna pruriens provides support for brain function, muscle health and libido. Mucuna pruriens has also been shown to have diuretic effects. It increases tissue resiliency and improves coordination. Mucuna can also increase testosterone levels, which in turn can lead to increased muscle mass and strength. It also supports the nervous and reproductive systems in the body. anti-oxidant activity of M. pruriens has been also demonstrated in vitro by its ability to scavenge DPPH radicals and reactive oxygen species. This is an excellent natural source of L-dopa and 5-hydroxy tryptophan (5-HT) Present study was designed to evaluate the anti-anxiety activity of Mucuna pruriens extract in Swiss albino mice. Three doses of Mucuna pruriens (100, 200,400 mg/kg, p.o.) and standard dose of Buspirone (5 mg/kg, i.p.) were used for evaluation of the anti-anxiety activity. The elevated plus maze (EPM) was used to take as a measure of antianxiety effect. Mucuna pruriens at the doses of 200 mg/kg and 400 mg/kg significantly reduced the time spent and no. of entries in closed arm, increased the time spent and entries into open arm in elevated plus maze (p<0.05) as compared to control group. These results indicate that MP may be possesses antianxiety property. Keywords: Anxiety, Elevated plus maze, Mucuna pruriens, Buspirone, Swiss Albino Mice.

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Effect of Piper Betle Leaf Extracts on Enhancement of Memory and Learning in Rodents

Journal of Evolution of Medical and Dental Sciences ◽

10.14260/jemds/2021/591 ◽

2021 ◽

Vol 10 (34) ◽

pp. 2900-2904

Author(s):

Roopa Prasad Nayak ◽

Uttara Krishna ◽

Chaitra S.R.

Keyword(s):

Learning And Memory ◽

Aqueous Extract ◽

Elevated Plus Maze ◽

Ethanolic Extract ◽

Screening Tests ◽

Piper Betle ◽

Swiss Albino Mice ◽

Plus Maze ◽

Albino Mice ◽

Memory Enhancing

BACKGROUND Medicinal plants have long been used for treating various diseases. Piper betle, an evergreen and perennial plant, is valued for its medicinal properties from ancient times. The purpose of this study was to assess the effect of aqueous extract and ethanolic extract of Piper betle leaf on animal models of learning and memory and compare it with piracetam. METHODS Piper betle leaves aqueous extract (PBAE) and ethanolic extract (PBEE) were administered to the Swiss albino mice at a dose of 100, 200 mg/kg body weight orally for 14 days to evaluate their effect on spatial learning and memory using the Hebb– William’s maze (HWM) and elevated plus maze (EPM). The study group included a control (distilled water) and a standard nootropic agent (Piracetam 150 mg/kg). RESULTS One-way analysis of variance (ANOVA) followed by Tukey Kramer’s multiple comparison test (P = 0.05) were used for comparison of groups and tabulated as mean ± SE. PBEE at a dose of 100 mg/kg and 200 mg/kg has shown significant memory enhancing activity, as indicated by a decrease in the TRC (time taken to reach the reward chamber) and TL (transfer latency) in both the screening tests of learning and memory. CONCLUSIONS PBEE possess potent memory enhancing property and can be considered as a potential drug for improving memory in the medical management of dementia, Alzheimer’s disease, and neurodegenerative disorders. KEY WORDS Piper betle L, Swiss Albino Mice, Hebb–Williams Maze (HWM), Elevated Plus Maze (EPM), Piracetam.

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Elevated Plus Maze and Y-Maze Behavioral Effects of Subchronic, Oral Low Dose Monosodium Glutamate in Swiss Albino Mice

IOSR Journal of Pharmacy and Biological Sciences ◽

10.9790/3008-0342127 ◽

2012 ◽

Vol 3 (4) ◽

pp. 21-27 ◽

Cited By ~ 11

Author(s):

Olakunle James ONAOLAPO

Keyword(s):

Low Dose ◽

Elevated Plus Maze ◽

Monosodium Glutamate ◽

Behavioral Effects ◽

Swiss Albino Mice ◽

Y Maze ◽

Plus Maze ◽

Albino Mice

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EVALUATION OF ANTI-ANXIETY EFFECT OF NIFEDIPINE COMPARED TO DIAZEPAM IN SWISS ALBINO MICE USING BEHAVIOURAL MODELS

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2020v12i5.37211 ◽

2020 ◽

pp. 6-9

Author(s):

PRAMOD SHANKPAL ◽

SHWETA SURVE

Keyword(s):

Elevated Plus Maze ◽

Chronic Administration ◽

Vehicle Control ◽

Box Model ◽

Behavioral Models ◽

Swiss Albino Mice ◽

Plus Maze ◽

Light Area ◽

Albino Mice ◽

Two Phases

Objective: The present study was undertaken to evaluate the acute and chronic anxiolytic effects of nifedipine in comparison to diazepam using in Swiss Albino mice using two behavioral models. Methods: 30 Swiss albino mice were divided into 5 groups with 6 mice in each group. The study was conducted in two phases to evaluate acute and chronic effects. The groups consisted of diazepam (1 mg/kg), 3 doses of nifedipine (2.6 mg/kg, 5.2 mg/kg and 10.4 mg/kg) and vehicle control. The Elevated Plus Maze (EPM) and Light and Dark box were used to evaluate the anti-anxiety effects. The number of entries and time spent in the open arm of the elevated plus-maze and in the light area of light and dark box model were noted and compared among the 5 groups. Observations were analyzed using ANOVA and post hoc Tukey's test. Results: Nifedipine (5.2 mg/kg and 10.4 mg/kg) significantly increased the number of entries and time spent in the open arm compared to vehicle control in the EPM test (p<0.001). Similarly, in the light and dark box test, nifedipine (5.2 mg/kg and 10.4 mg/kg) increased the number of entries and time spent in the light area compared to vehicle control (p<0.05). However, the low dose of nifedipine (2.6 mg/kg) did not exhibit significant findings. Conclusion: Two doses of nifedipine (5.2 mg/kg and 10.4 mg/kg) possess anti-anxiety effects both on acute and chronic administration in both elevated plus maze and light and dark box model.

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Catha Edulis Active Principle, Cathinone, Suppresses Motor Coordination, Accelerates Anxiety and Alters the Levels of Dopamine and its Metabolites in the Limbic Areas of Male Swiss Albino Mice

Acta Pharmaceutica ◽

10.2478/acph-2018-0038 ◽

2018 ◽

Vol 68 (4) ◽

pp. 485-495 ◽

Cited By ~ 1

Author(s):

Mohammed M. Safhi ◽

Mohammad Firoz Alam ◽

Gulrana Khuwaja ◽

Sohail Hussain ◽

Mohammed Hakeem Siddiqui ◽

...

Keyword(s):

Motor Performance ◽

Motor Coordination ◽

Elevated Plus Maze ◽

Control Group ◽

Active Principle ◽

Catha Edulis ◽

Swiss Albino Mice ◽

Elevated Plus Maze Test ◽

Plus Maze ◽

Albino Mice

Abstract Cathinone, the active principle of khat (Catha edulis), stimulates, excites and produces euphoric feelings in khat users. Locomotor and rearing activities, either individual or in groups, of male Swiss albino mice were decreased significantly compared to the control. Motor coordination tests (rotarod, rope climb and grip tests) have shown decreased motor performance in the mice treated with cathinone compared to the control. The elevated plus maze test has shown significant anxiety in the mice compared to the control. Contents of dopamine and its metabolite, homovanillic acid, were increased in the limbic areas compared to the control group. In contrast, contents of 3,4-dihydroxyphenyl acetic acid were depleted significantly and dose dependently compared to the control group in the limbic areas of mice. In conclusion, natural cathinone has depleted motor coordination, accelerated anxiety in mice and altered the contents of dopamine and its metabolites.

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