Protective effect ofLepidium sativumseed extract against hydrogen peroxide-induced cytotoxicity and oxidative stress in human liver cells (HepG2)

Isozeaxanthin under acidic conditions forms an allylic cation which reacts readily with thiol nucleophiles. With N-acetylcysteine as a nucleophile the products obtained are carotenoid-cysteine conjugates in which the amino acid moiety is attached to the carotenoid via sulphur in position 4. The water solubility of the products can be increased by deprotection of the amino group. The antioxidant activity of the products were examined on human liver cells under conditions of hydrogen-peroxide induced oxidative stress.

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HBx or HCV core gene expression in HepG2 human liver cells results in a survival benefit against oxidative stress with possible implications for HCC development

Chemico-Biological Interactions ◽

10.1016/j.cbi.2007.03.006 ◽

2007 ◽

Vol 168 (2) ◽

pp. 128-134 ◽

Cited By ~ 17

Author(s):

Tamara Severi ◽

Sara Vander Borght ◽

Louis Libbrecht ◽

Lucas VanAelst ◽

Frederik Nevens ◽

...

Keyword(s):

Oxidative Stress ◽

Gene Expression ◽

Survival Benefit ◽

Human Liver ◽

Core Gene ◽

Liver Cells ◽

Human Liver Cells

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In Vitro Assessment of Cadmium Bioavailability in Chinese Cabbage Grown on Different Soils and Its Toxic Effects on Human Health

BioMed Research International ◽

10.1155/2015/285351 ◽

2015 ◽

Vol 2015 ◽

pp. 1-12 ◽

Cited By ~ 4

Author(s):

Rukhsanda Aziz ◽

Muhammad Tariq Rafiq ◽

Zhenli He ◽

Di Liu ◽

Kewang Sun ◽

...

Keyword(s):

Oxidative Stress ◽

Human Health ◽

Chinese Cabbage ◽

Human Liver ◽

Liver Cells ◽

Leafy Vegetables ◽

Minimum Concentration ◽

Human Liver Cells ◽

Cadmium Bioavailability

The minimum concentration of cadmium (Cd), by Chinese cabbage grown on Cd contaminated soils that can initiate toxicity in human liver cells using in vitro digestion coupled with Caco-2/HL-7702 cell models was studied. Cadmium bioaccessibility in the gastric phase for yellow soil (YS) cabbage (40.84%) and calcareous soil (CS) cabbage (21.54%) was significantly higher than small intestinal phase with the corresponding values of 21.2% and 11.11%, respectively. Cadmium bioavailability was higher in YS cabbage (5.27%–14.66%) than in CS cabbage (1.12%–9.64%). Cadmium concentrations (>0.74 μg) transported from YS and CS cabbage were able to induce oxidative (MDA, H2O2) stress by inhibiting antioxidant (SOD, GPx) enzyme activities in human liver cells (HL-7702). Additionally the study revealed that the ingestion of Cd contaminated Chinese cabbage grown in acidic soil (yellow soil) weakened the antioxidant defense system under all levels of contamination (2, 6, and 9 mg·kg−1) which ultimately escalated the oxidative stress in liver cells; however, in case of CS cabbage, a marked oxidative stress was observed only at 9 mg kg−1Cd level of soil. Therefore, it is necessary to monitor Cd concentrations in leafy vegetables grown on acidic soils to minimize human health risk.

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