scholarly journals Expression of the Sod1 gene under conditions of experimental ethanol intoxication and administration of hepatoprotective drugs

2021 ◽  
pp. 132-137
Author(s):  
G. F. Mukhammadieva ◽  
A. B. Bakirov ◽  
D. O. Karimov ◽  
Ya. V. Valova ◽  
M. M. Ziatdinova ◽  
...  
Keyword(s):  
Rat Liver ◽  
Expression Level ◽  
Control Group ◽  
Reverse Transcription Pcr ◽  
Group 4 ◽  
White Rats ◽  
Group 5 ◽  
Group 2 ◽  
Prophylactic Use ◽  
Group Control Group

The aim of the study was to study the effect of hepatoprotective drugs on the expression of the Sod1 gene in rats with ethanol liver damage.Materials and methods. Male outbred white rats were used in the experiment. Five groups of animals were formed, 14 individuals each. Distilled water was administered to rats of the 1st group (control); Group 2 — ethanol at a dose of 5 g/kg of body weight; Group 3 — ethanol and heptor at a dose of 72 mg/kg; Group 4 — ethanol and mexidol at a dose of 50 mg/kg; Group 5 — ethanol and OMU at a dose of 50 mg/kg. The drugs were administered 1 hour before the introduction of ethanol. 24 and 72 hours after the introduction of ethanol (7 individuals), the animals were decapitated and the liver was removed. The expression level of the Sod1 gene was assessed using real-time reverse transcription PCR.Results. The fold change in Sod1 expression in rat liver after 24 h practically did not change in response to the introduction of ethanol to the animals. A tendency to a slight decrease was observed in relation to changes in the expression of Sod1 with the use of heptor and mexidol, while under the influence of OMU, the expression level increased moderately. After 72 h, the exposure to ethanol was accompanied by a slight decrease in the frequency of expression of the Sod1 gene. A similar trend was observed with respect to changes in Sod1 expression with the use of heptor, mexidol, and OMU.Conclusion. The results obtained indicate that the introduction of both ethanol and the prophylactic use of hepatoprotective drugs did not lead to significant changes in the level of Sod1 gene expression in rat liver. Additional studies are needed to identify the mechanisms of regulation of the antioxidant system, as well as the search for drugs that affect the transcriptional activity of genes.

scholarly journals Effectiveness of medical treatment in overcoming the ureteral double-J stent related symptoms

2012 ◽  
Vol 6 (6) ◽  
pp. 234 ◽  
Author(s):  
Ugur Kuyumcuoglu ◽  
Bilal Eryildirim ◽  
Murat Tuncer ◽  
Gokhan Faydaci ◽  
Fatih Tarhan ◽  
...  
Keyword(s):  
The Body ◽  
Control Group ◽  
Group 4 ◽  
Double J Stent ◽  
Urinary Tract Symptoms ◽  
Stone Treatment ◽  
Group 3 ◽  
Group 5 ◽  
Group 2

Background: We investigated whether the frequency of lower urinary tract symptoms (LUTS) increased in patients in whom double-J stents were applied. We also evaluated several medical therapy protocols to treat symptoms related with ureteral stents.Materials and Methods: A total of 108 patients, in whom unilateral double-j stent was applied during ureteral stone treatment, were included. Before the double-J stent was applied, all patients completed storage components of the “International Prostate Symptom Score” (IPSSs), quality of life components of the IPSS (IPSS-QOL) and “Overactive Bladder Questionnaire” (OABq) forms and scores were calculated. After the procedure, cases were randomized into 5 groups, an antiinflammatory was given to Group 1, spasmolytic to Group 2, anticholinergic to Group 3 and α-blocker to Group 4. No additional drug was given to Group 5 as this control group. During the fourth week of the procedure, IPSSs, IPSS-QOL and OABq forms were again completed and scores were compared with the previous ones.Results: When all the cases were evaluated, the IPSSs, IPSS-QOLand OABq scores of patients in whom the double-J stent was applied were statistically significantly higher the procedure. Compared to the control group, the cases where the double-J stent was applied showed a higher IPSSs, IPSS-QOL and OABq scores and none of the medical therapies could prevent this increase.Interpretation: The frequency of LUTS increased in cases where the ureteral stent was applied and discomfort continued as long as the stent stayed in the body.

scholarly journals Zingerone Ameliorates Hepatotoxicity

2020 ◽  
Vol 11 (1) ◽  
pp. 280-284 ◽  
Author(s):  
Julietpoornamathy J ◽  
Parameswari C.S.
Keyword(s):  
The Body ◽  
Control Group ◽  
Medical Sciences ◽  
Toxic Compounds ◽  
Group 4 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Insight Into ◽  
Group 1

In medical sciences, toxicity is an area wherein extensive studies have been carried to improve the diseases as well as to prevent. So, there is a high requirement for novel and improved alternative therapeutic strategies to manage diseases. The liver is the largest gland in the body, which executes several important mechanisms; it stores minerals and vitamins and releases them in periods of need. The main aim of this study was to give a closer insight into potent non- toxic compounds that is capable of modifying the responses. Animals were divided into five equal groups viz control (Group 1), administered with food and water ad libitum, (Group 2) administered with olive oil, (Group 3) administered with zingerone, (Group 4) administered with concanavalin A, (Group 5) administered with cyclosporine A followed by zingerone. Our results revealed significant changes in liver marker enzymes and liver histology of zingerone treated rats when compared to control rats.  A corollary, zingerone has no toxic effect on hepatocytes and was found to be safe at a dose of 10mg/kg b wt and also ameliorates hepatotoxicity.

scholarly journals The Effect of Zinc and Melatonin Supplementation on Lipid Peroxidation of Various Tissues of Rats with by DMBA-Induced Breast Cancer

2020 ◽  
Vol 11 (1) ◽  
pp. 7580-7588
Keyword(s):  
Breast Cancer ◽  
Lipid Peroxidation ◽  
Kidney Tissue ◽  
Female Rats ◽  
Control Group ◽  
Tissue Samples ◽  
Cancer Group ◽  
Group 5 ◽  
Group 2 ◽  
Group Control Group

The purpose of this study was to investigate the effects of zinc and melatonin administration on lipid peroxidation in various tissues in DMBA-induced breast cancer in female rats. A total of 42 recently weaned Wistar rats were divided into 5 groups in the study: Group 1 (Control), Group 2 (DMBA Control), Group 3 (DMBA+Zinc), Group 4 (DMBA+Melatonin), Group 5 (DMBA+Melatonin, and Zinc). MDA (malondialdehyde) and GSH (glutathione) levels were determined via the spectrophotometric method in the lung, liver, spleen, pancreas, and kidney tissue samples taken from experimental animals. The highest lung, liver, spleen, pancreas, and kidney tissue MDA levels were obtained in the DMBA-induced breast cancer group control group (G2) (p<0.05). MDA levels in DMBA+Zinc (G3), DMBA+Melatonin (G4), and DMBA+Melatonin and Zinc (G5) were significantly lower than group 2 (p<0.05). Similarly, lung, liver, spleen, pancreas, and kidney tissue GSH levels of DMBA+Zinc (G3), DMBA+Melatonin (G4), and DMBA+Melatonin and Zinc (G5) were significantly higher than that of Group 2 (p<0.05). The findings of the study indicated that increased lung, liver, spleen, pancreas, and kidney damage in DMBA-induced breast cancer is suppressed with the supplementation of zinc, melatonin, and combined zinc and melatonin.

Bond Integrity of Titanium Surface to Resin Cement After Conditioning with Different Photobiomodulataion Therapy (PBT)

2021 ◽  
Vol 11 (5) ◽  
pp. 970-975
Author(s):  
Khold Al Ahdal ◽  
Laila Al Deeb ◽  
Mohammed S. Bin-Shuwaish ◽  
Rana S. Al-Hamdan ◽  
Modhi Al Deeb ◽  
...  
Keyword(s):  
Titanium Alloy ◽  
Testing Machine ◽  
Resin Cement ◽  
Control Group ◽  
Sem Images ◽  
Group 4 ◽  
Modes Of Failure ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

The aim of the present study was to evaluate the efficacy of interfacial bonding between resin cement and titanium alloy conditioned with different Photobiomodulataion Therapy (PBT) in comparison with conventional regimes. Seventy-five samples of titanium bar were segmented polished; and based on conditioning regime divided into five groups (15 each specimens). Group 1: No treatment (control), Group 2: Sand blasting with 120 µm Al2O3, Group 3: Er,Cr:YSGG (ECL), Group 4: Er:YAG laser (EYL), Group 5: Nd:YAG laser (NYL). After different conditioning regimes, resin cement was mixed and applied. All specimens were thermocycled for 500 cycles and positioned on universal testing machine for shear bond strength testing. Modes of failure was assessed by a single examiner using stereomicroscope at 40× magnification. Three pairs of specimens in each group were sputter coated and scanning electron microscope (SEM) images were taken at 1000× magnification using 20 Kv. The highest SBS values were observed in group 2 (14.25±2.29 MPa) and the lowest bond values were displayed in group 1 (6.98 ±0.59 MPa). Titanium alloy abutments conditioned with different laser prototypes ECL (11.22±1.40 MPa), EYL (9.29±1.22 MPa) and NYL (9.12 ±1.84 MPa) exhibited comparable SBS (p > 0.05). Adhesive failures were primarily dominant among all experimental groups. Photo-biomodulation in the form of ECL lasers has the potential to be used as a supplement to sandblasting procedure in conditioning of cement retained titanium alloy implant abutments.

scholarly journals FE-SEM Evaluation of Dental Specimens Prepared by Different Methods forIn VitroContamination

2012 ◽  
Vol 2012 ◽  
pp. 1-5
Author(s):  
Vitor Cesar Nakamura ◽  
Simony Hidee Hamoy Kataoka ◽  
Giulio Gavini ◽  
Patrícia Helena Ferrari ◽  
Silvana Cai
Keyword(s):  
Control Group ◽  
Buffer Solution ◽  
Morphological Alterations ◽  
Ultrasonic Bath ◽  
Group 4 ◽  
Cement Removal ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

Objective. To evaluate through FE-SEM the cleanliness and dentinal alterations promoted by different methods of dental sample preparation.Methods. Twenty-five human single-rooted teeth were used. The teeth were cleaned and autoclaved in wet medium and randomly divided into 5 groups (), according to the preparation methods employed—control group: no solutions applied; group 1: cement removal and irrigation with 5.25% % EDTA for 4 minutes each; group 2: 17%  % NaOCl (4 minutes ultrasonic bath); group 3: cement removal and 17%     buffer solution + distilled water (10 minutes ultrasonic); group 4: 17%   NaOCl (3 minutes ultrasonic bath). Specimens were analyzed by field emission scanning electron microscope (FE-SEM), at 1500x magnification. Data were submitted to qualitative analysis according to a scoring system and submitted to Kruskal-Wallis test.Results. In ascending order, as to bind parameters, (i) cleanliness: control, group 2, group 3, group 5, and group 4, (ii) dentinal alterations: group 1, group 5, group 2, group 3, and group 4.Conclusion. The proposed protocol was suitable for subsequent microbiological contamination, because it showed less dentinal morphological alterations with increased removal of organic waste.

scholarly journals The Evaluation of Various Restoration Techniques on Internal Adaptation of Composites in Class V Cavities

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
D. Dionysopoulos ◽  
C. Papadopoulos ◽  
E. Koliniotou-Koumpia
Keyword(s):  
Room Temperature ◽  
Control Group ◽  
Class V ◽  
Group 4 ◽  
Restoration Techniques ◽  
Group 3 ◽  
Internal Adaptation ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

Aim.The aim of this study was to evaluate the effect of different restoration techniques on the formation of internal microgaps between materials and dentin in class V restorations.Materials and Methods.Twenty-five extracted human premolars were prepared with standardized class V cavity outlines (3 mm × 2 mm × 2 mm). The cavities were randomly divided into 5 groups of 10 cavities each and restored according to manufacturer’s instructions: Group 1: preheating (55°C) conventional composite (Filtek Z250), Group 2: flowable composite (Filtek Flow), Group 3: Filtek Flow + Filtek Z250 light-cured separately, Group 4: Filtek Flow + Filtek Z250 light-cured simultaneously, and Group 5 (control): Filtek Z250 at room temperature (23°C). The specimens were then thermocycled and cross-sectioned through the center of the restoration. Subsequently, impressions were taken, and epoxy resin replicas were made. The internal adaptation of the materials to the axial wall was analyzed under SEM.Results.The preheated Filtek Z250 (Group 1) showed better internal adaptation than the room temperature groups(P<0.05). The combination of Filtek Flow with Filtek Z250 which was light-cured separately (Group 3) exhibited better internal adaptation than control group(P<0.05).Conclusion.Different restoration techniques exhibit different behavior regarding internal adaptation to dentin after photopolymerization.

scholarly journals NADPH Oxidase 4 Signaling in a Ventilator-induced Lung Injury Mouse Model

2021 ◽  
Author(s):  
Sang Hoon Lee ◽  
Mi Hwa Shin ◽  
Ah Young Leem ◽  
Su Hwan Lee ◽  
Kyung Soo Chung ◽  
...  
Keyword(s):  
Lung Injury ◽  
Lavage Fluid ◽  
Lung Damage ◽  
Control Group ◽  
Group 4 ◽  
Ventilator Induced Lung Injury ◽  
Cell Counts ◽  
Group 5 ◽  
Group 2

Abstract For patients with acute respiratory distress syndrome, a ventilator is essential to supply oxygen to tissues, but it may also cause lung damage. We investigated the role of NOX4 in a ventilator-induced lung injury (VILI) model.Wild-type (WT) male C57BL/6J mice and NOX4 knockout (KO) male mice were divided into five groups: (1) control group; (2) high tidal ventilation (HTV) group: WT mice + HTV; (3) NOX4 KO group; (4) NOX4 KO with HTV group; (5) NOX4 inhibitor group: WT mice + HTV + NOX4 inhibitor. In addition, the relationship between EphA2 (which is related to lung injury) and NOX4 was investigated using EphA2 KO mice, and NOX4 levels in the bronchoalveolar lavage fluid (BALF) of 38 patients with pneumonia were examined.In the NOX4 inhibitor group, cell counts and protein concentrations from BALF were significantly lower than those in the HTV group (both, p<0.001). In the NOX4 KO group and the NOX4 inhibitor group, EphA2 levels were significantly lower than those in the HTV group (p<0.001). NOX4 levels were significantly higher in patients with pneumonia and patients who received ventilator treatment in the ICU.In the VILI model, it may be possible to block VILI using NOX4 antibodies.

scholarly journals Treatment of ovarian cysts in sows – a field trial

2008 ◽  
Vol 52 (No. 9) ◽  
pp. 413-418 ◽  
Author(s):  
S. Cech ◽  
R. Dolezel
Keyword(s):  
The Other ◽  
Ovarian Cysts ◽  
Control Group ◽  
Initial Examination ◽  
Interval Group ◽  
Group 4 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Group 1

Different procedures of treatment of large follicular ovarian cysts in 177 sows using GnRH, hCG and PGF2α are evaluated in this study. Ovarian cysts were diagnosed by transcutaneous ultrasonography, which was a part of routine pregnancy diagnosis. No treatment was performed in the control group (Group 1, <i>n</i> = 29); the method of treatment used in the other groups immediately after the diagnosis was intramuscular administration of lecirelin in doses 50 µg (Group 2, <i>n</i> = 28), 100 µg (Group 3, <i>n</i> = 27) and 200 µg divided into 2 equal doses administered at a 12-hour interval (Group 4, <i>n<i> = 25) and of hCG in doses 1 500 IU (Group 5, <i>n</i> = 23), 3 000 IU (Group 6, <i>n</i> = 21), and 250 µg of cloprostenol (Group 7, <i>n</i> = 24). Insemination rate (IR), conception rate (CR) in inseminated sows, pregnancy rate (PR = recovery rate), treatment-insemination interval (TII) and treatment-pregnancy interval (TPI) within 42 days after the initial examination were evaluated. In addition PR in groups of sows divided according to parity (1–3, 4–6 and ≥ 7) were also evaluated. IR and PR were higher in Group 4 (84.0% and 44.0%) and lower in Group 1 (17.2% and 6.9%) in comparison with the other groups (<i>P</i> < 0.001 and <i>P</i> < 0.05). CR, TII and TPI did not differ between the experimental groups. PR were similar in sows with different parity. The study proved a positive response in sows with large follicular ovarian cysts to the treatment consisting of 2 administrations of 100 µg GnRH at a 12-hour interval.

The Inhibitory Ability of Orobanche Extract on Calcium Oxalate Lithiasis in Male Albino Rats

2020 ◽  
Author(s):  
A. M. Kamal ◽  
M. S. Taha
Keyword(s):  
Treatment Group ◽  
Ethylene Glycol ◽  
Kidney Tissue ◽  
Inhibitory Effect ◽  
Control Group ◽  
Albino Rats ◽  
Potential Candidate ◽  
Group 4 ◽  
Group 5 ◽  
Group 2

The present study aimed to evaluate the inhibitory effect of Orobanche extract in ethylene glycol induced nephrolithiasis. Thirty male albino rats were divided into five groups each group contains 6 animals, group (1) control group, group (2) animals were supplied with 0.75% ethylene glycol in drinking water, group (3) animals were administrated Orobanche extract 3g/kg orally, group (4) animals were administrated Cystone 500 mg/kg in addition to 0.75% ethylene glycol, group (5) animals were administrated Orobanche extract 3g/kg orally in addition to 0.75% ethylene glycol the experiment continued for 28 days. Serum and the kidney homogenates were analyzed for various biochemical parameters and urine was examined microscopically for crystals. Orobanche treatment group and Cystone treatment group significantly decreased phosphorus, Calcium and Oxalate in kidney tissue of nephrolithiasis rats and significantly decreased kidney and liver marker in serum of nephrolithiasis rats. Conclusion this result revealed that Orobanche extract could be a potential candidate for phytotherapy against nephrolithiasis.

scholarly journals Aeromonas hydrophila in tilapia (Oreochromis niloticus) after the intake of aflatoxins

2013 ◽  
Vol 80 (4) ◽  
pp. 400-406 ◽  
Author(s):  
Samira Teixeira Leal de Oliveira ◽  
Gisele Veneroni-Gouveia ◽  
Janio Morais Santurio ◽  
Mateus Matiuzzi da Costa
Keyword(s):  
Aeromonas Hydrophila ◽  
Experimental Unit ◽  
Control Group ◽  
Feed Conversion ◽  
Group 4 ◽  
Randomized Design ◽  
Group A ◽  
Group 5 ◽  
Group 2 ◽  
The Impact

The objectives of this study were to assess the effect of the intake of aflatoxin on the development of tilapia and to evaluate the impact of inoculation with Aeromonas hydrophila on performance parameters, so these two individual tests were performed. One hundred and twenty fingerlings aged 35 days old, with mean weight of 1.55 ± 0.005 g and mean length of 5 cm were used in each test, distributed in 20 tanks. Each experimental unit consisted of a 60 L tank with six fingerlings. In the first experiment, increasing levels of aflatoxin (0.350, 0.757, 1.177 mg.kg feed-1) were used as treatments and, for the control group, a diet without aflatoxin was used in a completely randomized design with four treatments and five replicates. In the second experiment, a control group of Nile tilapia fingerlings was used and received a diet without aflatoxin, inoculated with saline solution (group 1) and Aeromonas hydrophila (group 2), as well as groups of animals fed on diets containing 0.350 mg.kg feed-1 of aflatoxin (group 3), 0.757 mg.kg feed-1 of aflatoxin (group 4) and 1.177 mg.kg feed-1 of aflatoxin (group 5), and these groups were inoculated with Aeromonas hydrophila. The survival rate and the total length of fingerlings were influenced by the treatments (p < 0.05). The synergistic action of aflatoxins and Aeromonas hydrophila was effective and caused the death of experimental fish, thus affecting feed conversion and length.

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