scholarly journals Fusarium fujikuroi species complex in Brazilian rice: unveiling increased phylogenetic diversity and toxigenic potential

2020 ◽  
Author(s):  
Camila Primieri Nicolli ◽  
Miriam Haidukowskic ◽  
Antonia Susca ◽  
Larissa Bitencourt Gomes ◽  
Antonio Logrieco ◽  
...  
Keyword(s):  
Geographical Distribution ◽  
Species Complex ◽  
Phylogenetic Diversity ◽  
The Other ◽  
Fusarium Fujikuroi ◽  
Phylogenetic Species ◽  
Frequent Species ◽  
New Knowledge ◽  
Fusarium Sp ◽  
Fusarium Fujikuroi Species Complex

Fusarium fujikuroi species complex (FFSC) species are commonly encountered infecting rice, but knowledge of the diversity and toxigenic potential of the species is lacking in Brazil, the largest rice-producing country outside Asia. One hundred FFSC isolates obtained from national rice were identified using morphology and phylogeny of TEF, CAL and TUB genes. Eight previously known and one new phylogenetic species were identified. Three species accounted for around 60% of the strains: F. fujikuroi (n = 23), F. proliferatum (n = 22) and F. verticillioides (n = 16). The less frequent species were F. volatile (n = 8), F. anthophilum (n = 6), F. pseudocircinatum (n = 4), F. sterilihyphosum (n = 2) and F. begoniae (n = 1). The new Fusarium sp. was represented by 18 isolates. All species produced at least one of the analyzed mycotoxins [beauvericin (BEA), fumonisins (FBs), moniliformin (MON) and enniatins (ENNs)]. BEA was produced by all species but F. verticillioides. The FBs (mainly FB1) were produced mostly by F. fujikuroi, F. proliferatum and F. verticillioides. F. begoniae and F. verticillioides did not produce ENNs and F. sterilihyphosum and F. begoniae did no produce MON, while the other species produced MON and ENNs. Our results contribute new knowledge of the diversity, geographical distribution and hosts of FFSC species.

scholarly journals Fusarium fujikuroi species complex in Brazilian rice: Unveiling increased phylogenetic diversity and toxigenic potential

2020 ◽  
Vol 330 ◽  
pp. 108667 ◽  
Author(s):  
Camila P. Nicolli ◽  
Miriam Haidukowski ◽  
Antonia Susca ◽  
Larissa B. Gomes ◽  
Antonio Logrieco ◽  
...  
Keyword(s):  
Species Complex ◽  
Phylogenetic Diversity ◽  
Fusarium Fujikuroi ◽  
Fusarium Fujikuroi Species Complex

Phylogenetic Diversity and In Vitro Susceptibility Profiles of Human Pathogenic Members of the Fusarium fujikuroi Species Complex Isolated from South India

2018 ◽  
Vol 183 (3) ◽  
pp. 529-540 ◽  
Author(s):  
Ananya Tupaki-Sreepurna ◽  
Vijayakishore Thanneru ◽  
Sangeetha Natarajan ◽  
Savitri Sharma ◽  
Anjana Gopi ◽  
...  
Keyword(s):  
Species Complex ◽  
Phylogenetic Diversity ◽  
South India ◽  
Fusarium Fujikuroi ◽  
In Vitro Susceptibility ◽  
Fusarium Fujikuroi Species Complex ◽  
Susceptibility Profiles

scholarly journals Fusarium musae from Diseased Bananas and Human Patients: Susceptibility to Fungicides Used in Clinical and Agricultural Settings

2021 ◽  
Vol 7 (9) ◽  
pp. 784
Author(s):  
Valeria Tava ◽  
Anna Prigitano ◽  
Paolo Cortesi ◽  
Maria Carmela Esposto ◽  
Matias Pasquali
Keyword(s):  
Species Complex ◽  
Crown Rot ◽  
Fusarium Fujikuroi ◽  
Clinical Settings ◽  
Skin Infections ◽  
Minimal Inhibitory Concentrations ◽  
Worldwide Population ◽  
Fusarium Fujikuroi Species Complex ◽  
Rot Disease ◽  
Systemic Infections

Fusarium musae belongs to the Fusarium fujikuroi species complex. It causes crown rot disease in banana but also keratitis and skin infections as well as systemic infections in immunocompromised patients. Antifungal treatments in clinical and agricultural settings rely mostly on molecules belonging to the azole class. Given the potential risk of pathogen spread from food to clinical settings, the goal of the work was to define the level of susceptibility to different azoles of a worldwide population of F. musae. Eight fungicides used in agriculture and five antifungals used in clinical settings (4 azoles and amphotericin B) were tested using the CLSI (Clinical and Laboratory Standards Institute) protocol methodology on 19 F. musae strains collected from both infected patients and bananas. The level of susceptibility to the different active molecules was not dependent on the source of isolation with the exception of fenbuconazole and difenoconazole which had a higher efficiency on banana-isolated strains. Minimal inhibitory concentrations (MICs) of the different molecules ranged from 0.12–0.25 mg/L for prochloraz to more than 16 mg/L for tetraconazole and fenbuconazole. Compared to the F. verticillioides, F. musae MICs were higher suggesting the importance of monitoring the potential future spread of this species also in clinical settings.

Design and validation of a robust multiplex polymerase chain reaction assay for MAT idiomorph within the Fusarium fujikuroi species complex

Mycologia ◽  
2019 ◽  
Vol 111 (5) ◽  
pp. 772-781 ◽  
Author(s):  
Amelia C. Montoya-Martínez ◽  
Gerardo Rodríguez-Alvarado ◽  
Sylvia P. Fernández-Pavía ◽  
Robert H. Proctor ◽  
Hye-Seon Kim ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Assay ◽  
Multiplex Polymerase Chain Reaction ◽  
Species Complex ◽  
Fusarium Fujikuroi ◽  
Chain Reaction ◽  
Fusarium Fujikuroi Species Complex ◽  
Polymerase Chain

scholarly journals Fusarium udum revisited: a common, but poorly understood member of the Fusarium fujikuroi species complex

2018 ◽  
Vol 18 (1-2) ◽  
pp. 107-117 ◽  
Author(s):  
Ludwig H. Pfenning ◽  
Maruzanete Pereira de Melo ◽  
Marileide Moreira Costa ◽  
Ailton Reis ◽  
Cleia Santos Cabral ◽  
...  
Keyword(s):  
Species Complex ◽  
Fusarium Fujikuroi ◽  
Fusarium Udum ◽  
Fusarium Fujikuroi Species Complex

Species composition of and fumonisin production by the Fusarium fujikuroi species complex isolated from Korean cereals

2018 ◽  
Vol 267 ◽  
pp. 62-69 ◽  
Author(s):  
Jung-Hye Choi ◽  
Seolhee Lee ◽  
Ju-Young Nah ◽  
Hee-Kyoung Kim ◽  
Ji-Seon Paek ◽  
...  
Keyword(s):  
Species Composition ◽  
Species Complex ◽  
Fusarium Fujikuroi ◽  
Fusarium Fujikuroi Species Complex

scholarly journals Fusarium fujikuroi Species Complex Associated With Rice, Maize, and Soybean From Jiangsu Province, China: Phylogenetic, Pathogenic, and Toxigenic Analysis

Plant Disease ◽  
2020 ◽  
Vol 104 (8) ◽  
pp. 2193-2201 ◽  
Author(s):  
Jianbo Qiu ◽  
Yunan Lu ◽  
Dan He ◽  
Yin-Won Lee ◽  
Fang Ji ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Species Complex ◽  
Fusarium Species ◽  
Phylogenetic Analyses ◽  
Germination Rate ◽  
Population Level ◽  
Jiangsu Province ◽  
Fusarium Fujikuroi ◽  
Rice Seed ◽  
Fusarium Fujikuroi Species Complex

Species belonging to the Fusarium fujikuroi species complex (FFSC) are of vital importance and are a major concern for food quantity and quality worldwide, as they not only cause serious diseases in crops but also produce various mycotoxins. To characterize the population structure and evaluate the risk of poisonous secondary metabolites, a total of 237 candidate strains were isolated from rice, maize, and soybean samples in Jiangsu Province, China. Species identification of the individual strain was accomplished by sequencing the translation elongation factor 1α gene (TEF-1α) and the fumonisin (FB) synthetic gene (FUM1). The distribution of Fusarium species among the different crops was observed. The maize seeds were dominated by F. proliferatum (teleomorph, Gibberella intermedia) and F. verticillioides (teleomorph, G. moniliformis), whereas F. fujikuroi (teleomorph, G. fujikuroi) was the most frequently isolated species from rice and soybean samples. In addition, phylogenetic analyses of these strains were performed, and the results suggested clear groups showing no obvious relationship with the origin source. FFSC species pathogenicity and toxigenicity were studied. All of the species reduced the rice seed germination rate, with no significant differences. F. fujikuroi showed two distinct patterns of influencing the length of rice seedlings, which were correlated with FBs and gibberellic acid synthesis. FBs were mainly produced by F. verticillioides and F. proliferatum. F. proliferatum and F. fujikuroi also produced moniliformin and beauvericin. The toxigenicity of F. andiyazi (teleomorph, G. andiyazi) was extremely low. Further analysis indicated that the sequence variations in TEF-1α and the differences in the expression levels of the toxin synthesis genes were associated with the diversity of secondary metabolites in F. fujikuroi strains. These findings provide insight into the population-level characterization of the FFSC and might be helpful in the development of strategies for the management of diseases and mycotoxins.

scholarly journals Cryptococcus gattii VGI Subtypes: Geographical Distribution, Molecular Traits, and Virulence Difference

2021 ◽  
Author(s):  
Xinying Xue ◽  
Wei Tang ◽  
Xuelei Zang ◽  
Weixin Ke ◽  
Yuxia Liu ◽  
...  
Keyword(s):  
Geographical Distribution ◽  
Species Complex ◽  
Reference Genome ◽  
De Novo ◽  
Cryptococcus Gattii ◽  
The Other ◽  
Molecular Type ◽  
Life Threatening

Abstract Background: As a life-threatening fungus, Cryptococcus gattii (C gattii) species complex is emerging worldwide. However, the geographical distribution, molecular traits, and virulence difference are poorly characterized in China.Results: From 2011 to 2017, we collected 32 strains of C gattii from 18 hospitals across China, of which 27 [84·4%] strains molecular traits were profiled by whole-genome sequencing (WGS) and multi-locus sequence typing (MLST) and compared with strains previously described in China from 2006 to 2020. Totally 119 clinical cases caused by C gattii strains (87 in previous reports and 32 in our study) distributed widely in 20 provincial-level administrative regions of China, of which 114 strains molecular types were obtained. The majority molecular type was VGI (81/114, 71·1%) and the other was VGII (33/114, 28·9%). Four major subtypes of VGI (VGIa, VGIb, VGIc, and VGId) were revealed from global C gattii VGI (n=308), respectively accounting for 52·9% (163/308), 36·0% (111/308), 3·9% (12/308), and 4·2% (13/308). The other nine strains could not be assigned to these four subtypes clearly. Our clinical data suggested that VGIb cases had a worse clinical outcome than VGIa, which was consistent with in vitro and in vivo experiments. In addition, a candidate virulence SNP on SOD2 in VGIa was initially identified by comparing high-quality de novo reference genome.Conclusions: The geographical distribution of C gattii species complex was first described in China. C gattii VGI could be clearly segregated into four major subtypes based on genomics profiles and VGIb was more virulent than VGIa in China. Our study suggests the molecular type of C gattii is necessary for personalized treatment in clinic.

scholarly journals Two new species of the Fusarium fujikuroi species complex isolated from the natural environment

2017 ◽  
Vol 110 (6) ◽  
pp. 819-832 ◽  
Author(s):  
Tarek A. A. Moussa ◽  
Hassan S. Al-Zahrani ◽  
Naif M. S. Kadasa ◽  
Sarah A. Ahmed ◽  
G. Sybren de Hoog ◽  
...  
Keyword(s):  
New Species ◽  
Natural Environment ◽  
Species Complex ◽  
Fusarium Fujikuroi ◽  
Two New Species ◽  
Fusarium Fujikuroi Species Complex

Fusarium Species from the Fusarium fujikuroi Species Complex Involved in Mixed Infections of Maize in Northern Sinaloa, Mexico

2014 ◽  
Vol 163 (6) ◽  
pp. 486-497 ◽  
Author(s):  
Karla Y. Leyva-Madrigal ◽  
Claudia P. Larralde-Corona ◽  
Miguel A. Apodaca-Sánchez ◽  
Francisco R. Quiroz-Figueroa ◽  
Priscila A. Mexia-Bolaños ◽  
...  
Keyword(s):  
Species Complex ◽  
Fusarium Species ◽  
Fusarium Fujikuroi ◽  
Mixed Infections ◽  
Fusarium Fujikuroi Species Complex
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