scholarly journals ISOLASI DAN IDENTIFIKASI BAKTERI SHIMBION SPONS PENGHASIL ENZIM AMILASE ASAL PANTAI MELAWAI BALIKPAPAN

2018 ◽  
Author(s):  
Ismail Marzuki ◽  
Alfian Noor ◽  
Nursiah La Nafie
Keyword(s):  
Amylase Activity ◽  
Microscopic Analysis ◽  
Isolation And Identification ◽  
Morphology Analysis ◽  
Condensation Activity ◽  
Transparent Zone ◽  
Very High ◽  
Sponge Symbionts ◽  
Macroscopic Analysis

Has been conducted research about isolation and identification bacterium symbionts sponge as producer enzyme amylase from Melawai Beach of Balikpapan. Targeting know is sponge type, symbionts bacterium group and test ability of sponge symbionts bacterium as amylase enzyme producer. Research expected to give information about existence of sponge and micro symbioning it’s from Melawai Beach. Research executed in four phase namely analysis sponge morphology, macroscopic analysis of isolate, microscopic analysis through coloration of bacterium symbionts, and test ability of symbion bacterium in yielding amylase enzyme. Result of morphology analysis is Callyspongia sp species enter in Callyspongiidae of family relatives, while perceived by microscopic and macroscopic perception of symbion bacterium in form of small bar, ellipse, surface flatten, colony disseminate, ruddle, fastening safranin colour designate the bacterium is negative gram. Perception of activity amylase enzyme at MA medium show colony have amylase activity marked with formed it’s of transparent zone at MA media colony if dropped by iodium condensation. Activity of amylase very high happened it thinning 10-2 marked with the existence of formed by zone of transparent very wide of around colony. Result of research concluded that t sponge is Callyspongia sp species, symbionts bacterium group is negative gram and bacterium represent producer of amylase enzyme

Use of activated carbon to remove undesirable residual amylase from refinery streams

2017 ◽  
pp. 96-103 ◽  
Author(s):  
Gillian Eggleston ◽  
Isabel Lima ◽  
Emmanuel Sarir ◽  
Jack Thompson ◽  
John Zatlokovicz ◽  
...  
Keyword(s):  
Activated Carbon ◽  
High Temperature ◽  
High Performance ◽  
Activated Carbons ◽  
Amylase Activity ◽  
Sugar Industry ◽  
End User ◽  
Customer Complaints ◽  
Carry Over ◽  
Very High

In recent years, there has been increased world-wide concern over residual (carry-over) activity of mostly high temperature (HT) and very high temperature (VHT) stable amylases in white, refined sugars from refineries to various food and end-user industries. HT and VHT stable amylases were developed for much larger markets than the sugar industry with harsher processing conditions. There is an urgent need in the sugar industry to be able to remove or inactivate residual, active amylases either in factory or refinery streams or both. A survey of refineries that used amylase and had activated carbon systems for decolorizing, revealed they did not have any customer complaints for residual amylase. The use of high performance activated carbons to remove residual amylase activity was investigated using a Phadebas® method created for the sugar industry to measure residual amylase in syrups. Ability to remove residual amylase protein was dependent on the surface area of the powdered activated carbons as well as mixing (retention) time. The activated carbon also had the additional benefit of removing color and insoluble starch.

scholarly journals Six-Year Evaluation of Thermal-Sprayed Coating of Zn/Al in Tropical Marine Environments

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Orlando Salas ◽  
Oladis Troconis de Rincón ◽  
Daniela Rojas ◽  
Adriana Tosaya ◽  
Nathalie Romero ◽  
...  
Keyword(s):  
Double Layer ◽  
Visual Inspection ◽  
Erosion Resistance ◽  
Microscopic Analysis ◽  
Marine Environments ◽  
Initial Weight ◽  
Layer System ◽  
Double Layer System ◽  
Sprayed Coating ◽  
Very High

The main objective of this research was to evaluate the performance of thermal-sprayed coating of Zn/Al (double layer) after six years of exposure, with and without the use of sealant (wash primer) in tropical marine environments of very high aggressiveness: La Voz Station (located at the Peninsula de Paraguaná/Falcón State) and Lake Maracaibo Crossing Station (located at Zulia State), in Venezuela. To that effect, carbon steel coupons (100 mm × 150 mm × 2 mm) were sprayed by flame process. The coupons were characterized by means of initial weight, thickness, metallographic, adherence, and roughness, being evaluated monthly by visual inspection during six years. After removal, the coupons were evaluated by microscopic analysis to determine the morphology of attack, microstructure, penetration of contaminants, composition, and morphology of corrosion products. The results showed that after six years, the double-layer system represents an excellent choice for corrosion protection of steel by combining the galvanic protection of zinc with the erosion resistance of aluminum. However, due to the erosion-corrosion effect, a sealant such as wash primer can be used in order to extend its service life.

scholarly journals ISOLATION AND IDENTIFICATION ON DEGRADATOR BACTERIUM OF PETROLEUM WASTE WHICH SYMBIONS WITH SPONGE FROM MELAWAI BEACH

2018 ◽  
Author(s):  
Ismail Marzuki ◽  
Alfian Noor ◽  
Nursiah La Nafie
Keyword(s):  
Research Method ◽  
Research Result ◽  
Sponge Species ◽  
Isolation And Identification ◽  
Petroleum Waste ◽  
Morphology Analysis ◽  
Type Activity ◽  
Yellow Color ◽  
The Media ◽  
Waste Degradation

The research has been done about isolation and identification on degradator bacterium of petroleum waste which symbions with sponge from Melawai Beach, Balikpapan. The research objectives; knowing sponge species, symbioning bacterium isolate and its ability of petroleum waste degradation. The research method were analysis of sponge Morphology analysis, isolation of symbions bacterium, identification, and activity testing at petroleum waste media which is coming from wells result of processing of PT. Chevron. The research result obtained the morphology analysis concluded that the sponges is Callyspongia sp. Bacterium symbion isolation obtained that two isolates which were given each symbols isolate A with the rounded shape and crem color whereas isolate B with yellow color and jagged shape. Coloring analysis of isolate obtained that isolate A is negative gram bacterium type and isolate B is positive gram bacterium type. Activity isolate testing at petroleum waste media, isolate A did not grow significantly at the media which marked with the existence of layer transparent on the media, isolate B growth significantly which marked with existence of solidity on the media, and the combination of isolate A+B also showed growth which marked with the existence of layer transparent and solidity on media. The conclusions research, sponge species is Callyspongia sp which contained two isolates bacterium namely negative gram bacterium and positive gram bacterium and both of the isolates can grow at media of petroleum waste.

scholarly journals Isolation and Identification of Amylase-producing Bacteria from Soil in Nasinuan Community Forest, Maha Sarakham, Thailand

2019 ◽  
Vol 12 (3) ◽  
pp. 1061-1068 ◽  
Author(s):  
Vijitra Luang-In ◽  
Manatchanok Yotchaisarn ◽  
Worachot Saengha ◽  
Piyachat Udomwong ◽  
Sirirat Deeseenthum ◽  
...  
Keyword(s):  
Enzyme Induction ◽  
Specific Activity ◽  
Amylase Activity ◽  
Optimal Conditions ◽  
Bacterial Isolates ◽  
Community Forest ◽  
Isolation And Identification ◽  
Rrna Sequencing ◽  
Bacillus Spp ◽  
Agricultural Industries

This study aimed to isolate and identify bacteria that can produce amylase enzyme from the unexplored Nasinuan Forest, Kantarawichai District, Mahasarakham Province, Thailand. Thirteen bacterial isolates with amylase-producing capacity on 1% starch agar were identified using 16S rRNA sequencing. Twelve bacteria were gram-positive, rod shaped and identified as Bacillus spp. and one bacterium with gram-negative and rod shaped character was Enterobacter cloacae. Their closest relatives were found in India, China, Korea, Indonesia, Argentina, Italy, Israel, USA, Argentina and South Africa. These bacteria were tested for specific amylase activity after 1-3 days enzyme induction with 1% starch at 37°C. The results showed the highest specific activity at day 2 incubation in the order: Bacillus cereus 3.5AL2 > 3.4AL1 > 1.4AL3 and thus 2-day enzyme induction was chosen for further analysis. Bacillus sp. 3.5AL2 was found to exhibit the highest specific amylase enzyme activity of 1.97 ± 0.41 U/mg protein at the optimal conditions of 60°C and pH 7.0 after 30 min incubation with 1% starch in 0.05 M PBS buffer. This amylase–producing bacterial strain offers great potential for applications in food and agricultural industries in Thailand.

Use of activated carbon to remove undesirable residual amylase from refinery streams

2017 ◽  
pp. 96-103
Author(s):  
Gillian Eggleston ◽  
Isabel Lima ◽  
Emmanuel Sarir ◽  
Jack Thompson ◽  
John Zatlokovicz ◽  
...  
Keyword(s):  
Activated Carbon ◽  
High Temperature ◽  
High Performance ◽  
Activated Carbons ◽  
Amylase Activity ◽  
Sugar Industry ◽  
End User ◽  
Customer Complaints ◽  
Carry Over ◽  
Very High

In recent years, there has been increased world-wide concern over residual (carry-over) activity of mostly high temperature (HT) and very high temperature (VHT) stable amylases in white, refined sugars from refineries to various food and end-user industries. HT and VHT stable amylases were developed for much larger markets than the sugar industry with harsher processing conditions. There is an urgent need in the sugar industry to be able to remove or inactivate residual, active amylases either in factory or refinery streams or both. A survey of refineries that used amylase and had activated carbon systems for decolorizing, revealed they did not have any customer complaints for residual amylase. The use of high performance activated carbons to remove residual amylase activity was investigated using a Phadebas® method created for the sugar industry to measure residual amylase in syrups. Ability to remove residual amylase protein was dependent on the surface area of the powdered activated carbons as well as mixing (retention) time. The activated carbon also had the additional benefit of removing color and insoluble starch.

scholarly journals Production and Biochemical Characterization of a High Maltotetraose (G4) Producing Amylase fromPseudomonas stutzeriAS22

2014 ◽  
Vol 2014 ◽  
pp. 1-11 ◽  
Author(s):  
Hana Maalej ◽  
Hanen Ben Ayed ◽  
Olfa Ghorbel-Bellaaj ◽  
Moncef Nasri ◽  
Noomen Hmidet
Keyword(s):  
Culture Medium ◽  
Biochemical Characterization ◽  
Amylase Activity ◽  
Potato Starch ◽  
Pseudomonas Stutzeri ◽  
Crude Enzyme Preparation ◽  
Amylase Production ◽  
Initial Ph ◽  
Very High

Amylase production and biochemical characterization of the crude enzyme preparation fromPseudomonas stutzeriAS22 were evaluated. The highestα-amylase production was achieved after 24 hours of incubation in a culture medium containing 10 g/L potato starch and 5 g/L yeast extract, with initial pH 8.0 at 30°C under continuous agitation at 200 rpm. The optimum temperature and pH for the crudeα-amylase activity were 60°C and 8.0, respectively. The effect of different salts was evaluated and it was found that bothα-amylase production and activity were Ca2+-dependent. The amylolytic preparation was found to catalyze exceptionally the formation of very high levels of maltotetraose from starch (98%, w/w) in the complete absence of glucose since the initial stages of starch hydrolysis (15 min) and hence would have a potential application in the manufacturing of maltotetraose syrups.

A study of alpha-amylase activity in tracheobronchial secretions of seriously ill patients with tracheostomies

1995 ◽  
Vol 109 (7) ◽  
pp. 640-643 ◽  
Author(s):  
V. Nandapalan ◽  
J. C. McIlwain ◽  
J. Hamilton
Keyword(s):  
Intensive Care Unit ◽  
Intensive Care ◽  
Amylase Activity ◽  
Intensive Care Unit Setting ◽  
Alpha Amylase ◽  
Seriously Ill Patients ◽  
High Level ◽  
Seriously Ill ◽  
Severe Pulmonary Disease ◽  
Very High

AbstractThis study was undertaken to assess any salivary aspiration in seriously ill patients with tracheostomies in an Intensive Care Unit setting. The alpha-amylase activity in the tracheostomies in an Intensive Care Unit setting. The alpha-amylase activity in the tracheobronchial secretions of 15 such patients were analysed to evaluate the incidence of salivary aspiration. None of the patients had clinical or radiological evidence of lung disorder at the time of the commencement of the study. Six out of 15 patients showed very high levels of alpha-amylase activity in their tracheobronchial secretions on Day 3 and all six subsequently developed severe chest infections. The other nine patients showed a low level of amylase activity in their secretions. Two patients in the latter group developed severe pulmonary disease. This study demonstrates that a high level of alpha-amylase activity in the tracheobronchial secretions of tracheotomized, ventilated patients indicates that salivary aspiration may be taking place, and further suggests that progressively increasing levels may indicate the likelihood of a major pulmonary complication developing.

scholarly journals Assessment of stallion semen morphology using two different staining methods, microscopic techniques, and sample sizes

2016 ◽  
Vol 60 (1) ◽  
pp. 99-104 ◽  
Author(s):  
Katarzyna Łącka ◽  
Stanisław Kondracki ◽  
Maria Iwanina ◽  
Anna Wysokińska
Keyword(s):  
Sperm Morphology ◽  
Staining Method ◽  
Microscopic Analysis ◽  
Sample Sizes ◽  
Morphology Analysis ◽  
Morphological Defects ◽  
Staining Methods ◽  
Microscopic Techniques ◽  
Semen Morphology

Abstract Introduction: The aim of this study was to propose the optimal methodology for stallion semen morphology analysis while taking into consideration the staining method, the microscopic techniques, and the workload generated by a number of samples. Material and Methods: Ejaculates from eight pure-bred Arabian horses were tested microscopically for the incidence of morphological defects in the spermatozoa. Two different staining methods (eosin-nigrosin and eosin-gentian dye), two different techniques of microscopic analysis (1000× and 400× magnifications), and two sample sizes (200 and 500 spermatozoa) were used. Results: Well-formed spermatozoa and those with major and minor defects according to Blom’s classification were identified. The applied staining methods gave similar results and could be used in stallion sperm morphology analysis. However, the eosin-nigrosin method was more recommendable, because it allowed to limit the number of visible artefacts without hindering the identification of protoplasm drops and enables the differentiation of living and dead spermatozoa. Conclusion: The applied microscopic techniques proved to be equally efficacious. Therefore, it is practically possible to opt for the simpler and faster 400x technique of analysing sperm morphology to examine stallion semen. We also found that the number of spermatozoa clearly affects the results of sperm morphology evaluation. Reducing the number of spermatozoa from 500 to 200 causes a decrease in the percentage of spermatozoa identified as normal and an increase in the percentage of spermatozoa determined as morphologically defective.

scholarly journals Macroscopic and microscopic analysis of the structures inserted in the posterior segment of the medial meniscus. How is the semimembranosus tendon involved in ramp lesions?

2021 ◽  
Vol 9 (2_suppl) ◽  
pp. 2325967121S0001
Author(s):  
Etienne Cavaignac ◽  
Remy Sylvie ◽  
Maxime Teulières ◽  
Andrea Fernandez ◽  
Bertrand Sonnery-Cottet
Keyword(s):  
Medial Meniscus ◽  
Sagittal Plane ◽  
Microscopic Analysis ◽  
Joint Capsule ◽  
Posterior Segment ◽  
The Body ◽  
Semimembranosus Tendon ◽  
Tibial Condyle ◽  
Meniscotibial Ligament ◽  
Macroscopic Analysis

Objectives: The anatomical description of the posterior segment of the medial meniscus is debatable. The aim of this study was to describe by macroscopic and microscopic analysis the histological nature of the posterior segment of the medial meniscus and the inserted structures (semimembranosus tendon and menisco-tibial ligament) Methods: Fourteen fresh knees were dissected. For each specimen, a stable anatomical piece was taken en bloc, including the medial femoral condyle, the medial tibial condyle, the entire medial meniscus, the cruciate ligaments and the joint capsule, and the distal insertion of the semimembranosus tendon was preserved in its entirety. At this stage, a macroscopic analysis was performed. The blocks were cut along the sagittal plane in order to isolate the distal insertion of the semimembranosus tendon on the posterior joint capsule and the posterior segment of the medial meniscus in the same section. Histological slides were produced from these samples and were microscopically analyzed. Results: In all patients, the macroscopic analysis showed direct semimembranosus tendon expansion and tendinous capsular expansion ending behind the posterior segment of the medial meniscus. It projected onto the joint capsule, on the meniscotibial ligament at the bottom and the meniscocapsular ligament at the top, but never ended directly in the meniscal tissue. On average, the tendon directly inserted 11 ± 2.8 mm below the articular surface of the tibial plateau. The length of the capsular expansion was 14.3 ± 4.4 mm. The meniscotibial ligament was inserted in the posterior-inferior edge of the posterior segment of the medial meniscus and the meniscocapsular ligament in the posterior-superior edge. There was a particularly vascularized adipocyte space delimited by the posterior segment of the medial meniscus, the meniscotibial ligament, the meniscocapsular ligament and the capsular expansion of the semimembranosus tendon. Conclusion: We repeatedly noted capsular expansion of the semimembranosus tendon that inserted behind the medial meniscus. There is an interposing zone between the tendon insertion and the body of the meniscus which creates a fragile zone. The capsular tendon expansion also inserts in the meniscotibial ligaments at the bottom and meniscocapsular ligaments at the top.

scholarly journals Solid Waste And Its Interference On The Water Quality Of The Igarapé¹ do Quarenta Educandos Basin - Manaus/Am

2021 ◽  
Vol 9 (11) ◽  
pp. 78-88
Author(s):  
Michael Raphael Soares Vieira ◽  
Aixa Braga Lopes ◽  
Eneida Guerra Silvestrim ◽  
Arlindo Almeida de Lima Filho ◽  
Kamila Vitoriano Gianello ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Water Quality ◽  
Electrical Conductivity ◽  
Solid Waste ◽  
Sampling Site ◽  
The City ◽  
Waste Accumulation ◽  
Very High ◽  
Macroscopic Analysis

The “Igarapé do Quarenta”, a stream in Educandos basin, is categorized today as the "worst" and most polluted of the city Manaus, Amazonas, due to the accumulation of solid waste and contamination by heavy metals along its course. The main objective of this research was to verify the influence of solid waste in critical points of the Igarapé do Quarenta, on the water quality of the stream. The area of solid waste accumulation ("garbage") and the water quality in the stream and around and under the bridges of the Igarapé do Quarenta, between the springs (Armando Mendes and Zumbi neighborhoods) and the mouth (Educandos), in the Amazon "summer" and "winter"," were quantified. Water quality was evaluated at each sampling site. The macroscopic analysis demonstrated its importance for the feasibility diagnosis, finding that two of the three springs found are degraded and disturbed. The pH and electrical conductivity values in the sample sites visited, in the upper and middle, of the Igarapé do Quarenta, were very high values in relation to the maximum values recorded in a natural environment in other water streams of Manaus.

Sign in / Sign up

Export Citation Format

Share Document