ISOLATION AND IDENTIFICATION ON DEGRADATOR BACTERIUM OF PETROLEUM WASTE WHICH SYMBIONS WITH SPONGE FROM MELAWAI BEACH
The research has been done about isolation and identification on degradator bacterium of petroleum waste which symbions with sponge from Melawai Beach, Balikpapan. The research objectives; knowing sponge species, symbioning bacterium isolate and its ability of petroleum waste degradation. The research method were analysis of sponge Morphology analysis, isolation of symbions bacterium, identification, and activity testing at petroleum waste media which is coming from wells result of processing of PT. Chevron. The research result obtained the morphology analysis concluded that the sponges is Callyspongia sp. Bacterium symbion isolation obtained that two isolates which were given each symbols isolate A with the rounded shape and crem color whereas isolate B with yellow color and jagged shape. Coloring analysis of isolate obtained that isolate A is negative gram bacterium type and isolate B is positive gram bacterium type. Activity isolate testing at petroleum waste media, isolate A did not grow significantly at the media which marked with the existence of layer transparent on the media, isolate B growth significantly which marked with existence of solidity on the media, and the combination of isolate A+B also showed growth which marked with the existence of layer transparent and solidity on media. The conclusions research, sponge species is Callyspongia sp which contained two isolates bacterium namely negative gram bacterium and positive gram bacterium and both of the isolates can grow at media of petroleum waste.