scholarly journals Phytochemical Analysis and Antimicrobial Activity of Ethanolic Extract of Dried Fruit Rind of Garcinia gummi-gutta

2021 ◽  
Vol 7 (2) ◽  
pp. 81-85
Author(s):  
A Rehna ◽  
◽  
KP Neethu ◽  
AK Deepa ◽  
◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Intestinal Parasites ◽  
Ethanolic Extract ◽  
Soxhlet Extraction ◽  
Phytochemical Analysis ◽  
Food Borne ◽  
Vibrio Parahemolyticus ◽  
Dried Fruit ◽  
Fruit Rind

Garcinia gummi-gutta is an evergreen medium sized tree of the family Clusiaceae with remarkable traditional value which has been indicated for the treatment of bowel complaints, rheumatism, intestinal parasites and also as a culinary and preservative agent for fish preparations in Southern India and Srilanka. The extract of dried fruit rind of Garcinia gummi-gutta obtained through Soxhlet extraction using ethanol was subjected to preliminary phytochemical analysis along with in vitro evaluation of antimicrobial activity against common food borne pathogens. Preliminary phytochemical analysis of the rind extract of the plant showed the presence of phytochemical constituents such as alkaloids, phenolic compounds, steroids, tannins, flavonoids, glycosides, diterpenes and triterpenes. The plant extracts were evaluated for the antimicrobial activity by in vitro agar well-diffusion at a dose rate of 5mg/ml, 10 mg/ml and 15mg/ml against the microbial strains namely Staphylococcus aureus, Vibrio parahemolyticus, Listeria monocytogenus and Salmonella tymphimurium. The results of the antimicrobial activity showed effective inhibitory activity against all the tested organisms in a dose dependant manner.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTI-MICROBIAL ACTIVITY OF ETHANOLIC EXTRACT OF STEMS OF THE PLANT TAGETES ERECTA L. (FAMILY-ASTERACEAE)

2018 ◽  
Vol 10 (4) ◽  
pp. 64
Author(s):  
Jafar Ali ◽  
Dibyendu Shil
Keyword(s):  
Antimicrobial Activity ◽  
Microbial Activity ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Tagetes Erecta ◽  
Phytochemical Analysis ◽  
Standard Drug ◽  
Phytochemical Constituents ◽  
Tagetes Erecta L

Objective: To estimate the anti-microbial activity of ethanolic extract of Tagetes erecta L. (family-Asteraceae) in association with phytochemical analysis.Methods: The ethanolic extract of the stems of TageteseErecta L. was prepared and investigate for phytochemical constituents using standard methods. Antimicrobial activity of ethanolic extract of has been carried out against one Gram positive bacteria-Staphylococcus aureus and one Gram negative bacteria-Escherichia coli, and the antifungal activity of the plant extract was evaluated on Candida albicans species. The testing was done by the disc diffusion method to visualize the zone of inhibition of ethanolic extract was compared with that of standard drug i.e., Ciprofloxacin for anti-bacterial activity and Terbinafine for anti-fungal activity. The MIC was determined by the microdilution method (Cup-plate method).Results: The present investigation shows the phytochemical analysis, antimicrobial activity of the ethanolic extract of the stems of Tagetes erecta L. Various phytochemical analysis confirmed the presence of alkaloids, saponin glycosides, flavonoids, phenol, proteins and amino acids, cardiac glycosides, steroids, tanin and phenolic compounds. The anti-microbial activity of the ethanolic extract of the plant showed significant results against all three of the test organisms in compare to the standard drugs.Conclusion: The present study concluded that ethanolic extract of-of the stems of Tagetes erecta L. Proved the existence of phytochemical constituents. The ethanolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards and it can be useful for the development of the antibiotics.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACT OF WHOLE AERIAL PART OF THE HERB LEUCAS PLUKENETII SPRENG (FAMILY-LAMINACEAE)

2017 ◽  
Vol 9 (3) ◽  
pp. 87
Author(s):  
Uttam Borah ◽  
Biswajit Dash ◽  
Suvakanta Dash ◽  
Lawrence Kalita
Keyword(s):  
Antimicrobial Activity ◽  
Aerial Part ◽  
Ethanolic Extract ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Bacterial Strains ◽  
Standard Methods ◽  
Aerial Parts ◽  
Phytochemical Constituents

Objective: To evaluate the antimicrobial activity of ethanolic extract of Leucas Plukenetii Spreng (family-laminaceae) in conjugation with phytochemical analysis.Methods: The ethanolic extract of whole aerial parts of the herb Leucas Plukenetiispreng (family-laminaceae) was prepared and analysed for phytochemical constituents using standard methods. The antimicrobial activity of the plant extract was examined against 2 bacterial strains among one is gram positive and other is gram negative and 2 fungal strains using agar well diffusion method.Results: The present investigation shows the phytochemical analysis, antimicrobial activity of the ethanolic extract of the plant Leucas Plukenetii Spreng. Various phytochemical analyses revealed the presence of alkaloids, saponin, flavonoids, carbohydrates, glycosides, Steroids, proteins and amino acids and tannins. The antimicrobial activity of the ethanolic extract of the plant showed significant result against all the of the test organisms.Conclusion: The present study concluded that ethanolic extract of whole aerial parts of the Leucas Plukenetii Spreng contains the high presence of phytochemicals. The ethnaolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTI-MICROBIAL ACTIVITY OF ETHANOLIC EXTRACTS OF FRUITS OF ANNONA RETICULATA AGAINST STANDARD PATHOGENIC STRAINS

2018 ◽  
Vol 10 (4) ◽  
pp. 59
Author(s):  
Sujata Paul ◽  
Biswajit Dash ◽  
Aditya Jyoti Bora ◽  
Bikash Gupta
Keyword(s):  
Microbial Activity ◽  
Ethanolic Extract ◽  
Soxhlet Extraction ◽  
Phytochemical Analysis ◽  
Disc Diffusion ◽  
Phytochemical Screening ◽  
Bacterial Strains ◽  
Phytochemical Constituents ◽  
Annona Reticulata

Objective: To evaluate the anti-microbial activity of ethanolic extract of fruits of Annona reticulata (family-Annonaceae) in conjugation with phytochemical analysis.Methods: The ethanolic extract of fruits of Annona reticulata (family-Annonaceae) was prepared by Soxhlet extraction and analysed for phytochemical constituents using standard methods. The anti-microbial activity of the plant extract was examined against bacterial strains and fungal strains using disc diffusion method.Results: The present investigation shows the phytochemical analysis, anti-microbial activity of the ethanolic extract of the plant Annona reticulata. Various phytochemical analyses revealed the presence of alkaloids, saponin, flavonoids, carbohydrates, glycosides, steroids, proteins and amino acids and tannins. The anti-microbial activity of the ethanolic extract of the plant showed significant result against all the of the test organisms.Conclusion: The present study concluded that ethanolic extract of fruits of the Annona reticulata contain high presence of phytochemicals. The ethanolic extract of the plant was found to possess promising anti-microbial activity when compared with the standards.

scholarly journals PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTI-MICROBIAL ACTIVITY OF ETHANOLIC EXTRACT OF DELONIX REGIA (HOOK) RAF. BARK (FAMILY-LEGUMINOSAE)

2018 ◽  
Vol 10 (4) ◽  
pp. 43
Author(s):  
Bikash Gupta ◽  
Biswajit Dash ◽  
Sujata Paul ◽  
Aditya Bora
Keyword(s):  
Antimicrobial Activity ◽  
Microbial Activity ◽  
Ethanolic Extract ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Delonix Regia ◽  
Gram Positive Bacteria ◽  
Phytochemical Constituents ◽  
Anti Fungal

Objective: To estimate the anti-microbial activity of ethanolic extract of Delonix regia (Hook.) Raf (family-Leguminosae) in association with phytochemical analysis.Methods: The ethanolic extract of the bark of Delonix regia (Hook.) Raf was prepared and investigate for phytochemical constituents using standard methods. Antimicrobial activity of ethanolic extract of were carried out against one Gram positive bacteria= Staphylococcus aureus and one Gram negative bacteria= Escherichia coli. The anti-fungal activity of the plant extract was evaluated on Candida albicans. The testing was done by the disc diffusion method. A zone of inhibition of ethanolic extract was compared with that of standard Amikacin for anti-bacterial activities and Fluconazole for anti-fungal activity.Results: The present investigation shows the phytochemical analysis, antimicrobial activity of the ethanolic extract of the bark of Delonix regia (Hook.) Raf. Various phytochemical analyses revealed the presence of alkaloids, anthraquinone, phenol, terpenes, cardenolides, phlobatannins, tannin, chalcones, steroids. The anti-microbial activity of the ethanolic extract of the plant showed significant results against all three of the test organisms.Conclusion: The present study concluded that ethanolic extract of the bark of Delonix regia (Hook.) Raf contains the high existence of phytochemicals. The ethanolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards.

scholarly journals Phytochemical Analysis, Antioxidant and Anti-Inflammatory Activity of Calyces from Physalis peruviana

2014 ◽  
Vol 9 (11) ◽  
pp. 1934578X1400901 ◽  
Author(s):  
Reina M. Toro ◽  
Diana M. Aragón ◽  
Luis F. Ospina ◽  
Freddy A. Ramos ◽  
Leonardo Castellanos
Keyword(s):  
Antioxidant Activities ◽  
Folk Medicine ◽  
Ethanolic Extract ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Physalis Peruviana ◽  
Edema Model ◽  
Anti Inflammatory ◽  
Bio Assay

Physalis peruviana calyces are used extensively in folk medicine. The crude ethanolic extract and some fractions of calyces were evaluated in order to explore antioxidant and anti-inflammatory activities. The anti-inflammatory activity was evaluated by the TPA-induced ear edema model. The antioxidant in vitro activity was measured by means of the superoxide and nitric oxide scavenging activity of the extracts and fractions. The butanolic fraction was found to be promising due to its anti-inflammatory and antioxidant activities. Therefore, a bio-assay guided approach was employed to isolate and identify rutin (1) and nicotoflorin (2) from their NMR spectroscopic and MS data. The identification of rutin in calyces of P. peruviana supports the possible use of this waste material for phytotherapeutic, nutraceutical and cosmetic preparations.

TOPICAL GEL OF EXTRACT OF MANGIFERA INDICA L. SEED KERNELS; AN INDIGENOUS PLANT WASTE FOR POTENTIAL ANTI-ACNE ACTIVITY

INDIAN DRUGS ◽  
2021 ◽  
Vol 58 (05) ◽  
pp. 73-76
Author(s):  
Leena Varhadi ◽  
◽  
Shweta Kitture ◽  
Namita Desai ◽  
Aparna Palshetkar
Keyword(s):  
Antimicrobial Activity ◽  
Mangifera Indica ◽  
Waste Product ◽  
Ethanolic Extract ◽  
Seed Kernel ◽  
In Vitro Antimicrobial Activity ◽  
Topical Gel ◽  
Plant Waste ◽  
Indigenous Plant

The present study was aimed at the development of topical gel of extract of Mangifera indica L. seed kernel and evaluation of its in vitro antibacterial activity. Various extracts using different solvents were prepared and evaluated for in vitro activity against Propionibacterium acnes. Ethanolic extract of M. indica L. seed kernel exhibited the highest in vitro antimicrobial activity and was considered further. Topical gels of this ethanolic extract were prepared using different polymers such as Carbopol 974P, Xanatural 180 and Methocel K15M in varying concentrations. The topical gel of 10 % w/w ethanolic extract of M. indica L. seed kernel with 2% w/w Carbopol 974P was uniform and yellowish transparent with satisfactory spreadability. The developed gel showed greater in vitro antimicrobial activity as compared to marketed herbal cream. These results indicate the potential of topical gel of ethanolic extract M. indica L. seed kernel as anti-acne formulation developed from plant waste product.

scholarly journals In vitro antimicrobial activity of mexican plants on bovine mastitis bacteria: preliminary studies

2020 ◽  
Vol 36 (1) ◽  
Author(s):  
Mariana Macías Alonso ◽  
Julio C. López Salazar ◽  
Soraya Osegueda Robles ◽  
Iván Córdova Guerrero ◽  
Fernanda Ledezma García ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Low Cost ◽  
Bovine Mastitis ◽  
Ethanolic Extract ◽  
Clinical Mastitis ◽  
Potential Candidate ◽  
Salmonella Spp ◽  
Shigella Spp ◽  
In Vitro Antibacterial Activity

The present study aimed to evaluate the efficacy of traditionally used antibacterial plants (Euphorbia prostrata, Schinus molle, Brickellia veronicaefolia and Lepidium virginicum) in Santa Rosa Range in Guanajuato against major mastitis-causing pathogens. The selected plants were phytochemically screened for alkaloids, flavonoids, polyphenols, saponins, anthraquinone, cardiac glycosides, steroids and terpenoids. Moreover, to achieve the main purpose of the present research, extracts of selected plants were tested for in vitro antibacterial activity against Staphylococcus aureus, Escherichia coli, Shigella spp., Bacillus subtilis, Clavibacter spp., Proteus vulgaris and Salmonella spp. All these bacterial were isolated from clinical mastitis in dairy cows. The results indicated that the ethanolic extract of the aerial part of L. virginicum exhibited the strongest antimicrobial activity, and it could be potential candidate specie for the development of novel veterinary drugs with low cost and fewer side effects.

scholarly journals Study on antibacterial activity of Calotropis procera

2014 ◽  
Author(s):  
Muhammad Muzammal
Keyword(s):  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Methanolic Extract ◽  
Ethanolic Extract ◽  
Salmonella Typhi ◽  
Calotropis Procera ◽  
Phytochemical Analysis ◽  
Zone Of Inhibition ◽  
Crude Extracts ◽  
Mic Value

Comparative study of plant extracts crude and aqueous, methanolic and ethanolic with antibiotics, provide evidence that calotropis procera extracts has the similar antibacterial activity as these antibiotics against test pathogens i.e. Salmonella typhi and E.coli. The analysis of antimicrobial activity of aqueous, methanolic and ethanolic extract of leaves and flower of Calotropis procera was carried out in disc method and also determined MIC value at 600nm through optical density using spectrophotometer. The zone of inhibition produced by extracts was examined and compares it with zone produced by antibiotics. The effect exhibited by ethanolic extract of leaves and flower was significantly greater than the aqueous and methanolic extract of leaves and flower. Crude extracts i.e. latex, leaves, fruit and flower crude extracts. Among them, flower crude extracts shows similar zone of inhibition to test anitibiotics.while in MIC value, we made different concentration of extracts and antibiotics.i.e for crude we made 25%, 50%.75% and 100% concentration of crude juice and for the aqueous, methanolic and ethanolic we made 0.5mg/mL,1mg/mL,2mg/mL,3mg/mL,4mg/mL, 5mg/mL and 6mg/mL in Dimethyl salfoxide, and same antibiotics concentration. Aqueous leaves extracts show MIC at 0.5mg/ml against E.coli while against Salmonella it shows MIC at 1mg/ml... We also determine phytochemical analysis for presences of different compounds in crude extracts. The obtained results provide a support for the use of Calotropis procera, in traditional medicine and suggest its further advance investigation.

scholarly journals A Comparative Study of Antimicrobial Activity of Ethanolic Extract of Tecoma stans and Abutilon indicum Leaves: An In vitro Study

2021 ◽  
pp. 609-615
Author(s):  
M. Binigha ◽  
R. Gayatri Devi ◽  
J. Selavaraj ◽  
A. Jothi Priya
Keyword(s):  
Antimicrobial Activity ◽  
Agar Plate ◽  
Ethanolic Extract ◽  
Flowering Plant ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
Zone Of Inhibition ◽  
The Family ◽  
Abutilon Indicum

Tecoma stans is a flowering plant belonging to the family Bignoniaceae. It is the floral emblem of the Bahamas. Abutilon indicum belongs to the family Malvaceae. It is also called Indian Mallow. Tecoma stans and Abutilon indicumhave antimicrobial activity and can be used in producing antibiotics. There is an urgent need to produce new antibiotics as there is an increase in the development of pathogen resistant drugs. The aim of this study was to compare the antimicrobial activity of ethanolic extract of Tecoma stansand Abutilonindicum leaves. E.faecalis bacteria were maintained in nutrient agar slopes. The powder of the two herbs, were subjected to extraction by Soxhlet extractor and was concentrated to dry residue by Agar well diffusion method. The nutrient broth was inoculated with bacterial strains. The culture was adjusted to 0.5McFarland turbidity standard. Lawn culture was done in Muller-Hinton agar plate and then the plates were dried. A 6 mm diameter well is bored for different concentrations. The extracts were introduced into the well and allowed to stand for 24 hrs. The antibacterial activity was determined by measuring the zone of inhibition. The present finding on the antimicrobial potential of Abutilon indicum and Tecoma stands showed that the two plants have growth inhibitory activity against oral pathogen E. faecalis. Abutilon indicum has a zone of inhibition greater than Tecoma stans. Abutilon indicum exhibited better activity compared to Tecoma stans.

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