scholarly journals Effect of sugarcane genotypes and 2, 4-dichlorophenoxy acetic acid on callus induction

2018 ◽  
pp. 237-242
Author(s):  
M. Subedi ◽  
B. R. Ojha ◽  
S. K. Ghimire ◽  
B. K. Joshi ◽  
R. K. Niroula ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Genetic Engineering ◽  
In Vitro Culture ◽  
Callus Induction ◽  
Optimum Dose ◽  
Synthetic Seed ◽  
The Media ◽  
Dichlorophenoxy Acetic Acid ◽  
Different Doses

 Callus induction is an important step in in-vitro culture valued for micropropagation, somaclonal variation, mutagenesis, synthetic seed production, transformation and genetic engineering. Callusing response of ten sugarcane genotypes viz., BO-110, BO-134, Cose-95422, Cose-98232, Cose-92423, UP-9742, BO-91, BO-135, and Cose-98255, were studied under three different doses (2, 3 & 4 mg L-1) of 2,4-D supplemented in the callus induction media. Callus induction was found to depend on both genotype and 2,4-D concentration in the media. The genotype UP-9742 was most amenable to callus induction (67.50%) followed by Cose-97182 (56.25%) and Cose-92423 (51.00%). While, the media supplemented with 3 mg L-1 2,4-D was found more effective to trigger callus induction in general. Yet, the optimum dose of 2,4-D to be used depends on the interaction of the specific genotype. Journal of the Institute of Agriculture and Animal Science.Vol. 33-34, 2015, page: 237-242

scholarly journals Sterilization procedure and callus regeneration in black turmeric (Curcuma caesia)

2019 ◽  
Vol 6 (49) ◽  
Author(s):  
A. S. Abubakar ◽  
R. N. Pudake
Keyword(s):  
Acetic Acid ◽  
Callus Induction ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Surface Sterilization ◽  
To Come ◽  
Dichlorophenoxy Acetic Acid ◽  
And Control ◽  
Rhizome Explants

Sterilization procedure, media composition, explants selection and control of physical environment are critical for successful cultures and callus induction with surface sterilization being very challenging in most plants. Five different sterilization methods were evaluated to come up with the best for subsequent use to establish an in vitro regeneration method for the induction of callus in Curcuma caesia using excised leaf and rhizome explants. Murashige and Skoog (MS) media supplemented with various concentration of 2,4-Dichlorophenoxy acetic acid (2,4-D)/Indole-3-acetic acid (IAA) (0.5- 5.0mg/L), singly or in combination with Benzyl aminopurine (BAP)/Kinetin (KIN) (0.1-5.0mg/L), 0.3% sucrose and 0.08% agar were used. The result of the sterilization procedures showed 15% NaHClO3 (5min) + 70% Ethanol (30s) + 0.1% HgCl2 (5min) to be the most effective in controlling contamination in C. caesia among all the treatments tested. The response to callus induction was found to depend on the type of explants used and growth regulators combination. Leaf explants gave the highest percentage of callus induction. Highest percentage of callus induction (66.70%) was obtained in the growth regulator combination of 2, 4-D (0.5mg/L) + BAP (0.1mg/L) and least (14.29%) in IAA (2.0mg/L) + BAP (0.5mg/L). Equal and higher concentration of 2, 4-D + BAP of 5.0mg/L each also provided better result (40.00%). No callus was obtained in all the single concentration of 2, 4-D used.

scholarly journals Effect of phytohormones on shoot apex and leaf explants of Withania somnifera (Ashwagandha)

2016 ◽  
Vol 8 (1) ◽  
pp. 412-415 ◽  
Author(s):  
Archana Rani ◽  
M. Kumar ◽  
Sanjeev Kumar
Keyword(s):  
Acetic Acid ◽  
Shoot Apex ◽  
Callus Induction ◽  
Withania Somnifera ◽  
Leaf Explants ◽  
Ms Medium ◽  
Mass Propagation ◽  
Best Response ◽  
Dichlorophenoxy Acetic Acid

An efficient protocol for callus induction of Withania somnifera through in vitro culture of shoot apex and leaf explant was standardized. Of the various combinations of phytohormones evaluated, MS media supplemented with 6-furfuryl aminopurine (KIN) 0.5 mg/l + 2,4-dichlorophenoxy acetic acid (2, 4-D) 2.0 mg/l was found to be bestfor mean callus induction (86%) in leaf explants after 6 weeks of culture and in case of shoot apex expant the best response and growth of callusing was observed on MS medium supplemented with 2,4-D 1.0 mg/l + BAP 2.0 mg/l (77%).The response of callus growth increases gradually with the reductions in concentration of KIN in culturemedium of both the explants. This protocol might be used in further research for mass propagation of W. somnifera via indirect regeneration methods.

scholarly journals TEKNIK BUDIDAYA IN VITRO Eleutherine sp. (Bawang Sabrang)

2016 ◽  
Vol 11 (3) ◽  
pp. 341
Author(s):  
Djadja Siti Hazar Hoesen
Keyword(s):  
Acetic Acid ◽  
Plant Growth Regulator ◽  
Ms Medium ◽  
Benzyl Adenine ◽  
Soil Medium ◽  
Shoot Buds ◽  
The Media ◽  
Initiation Stage ◽  
Dichlorophenoxy Acetic Acid

Vegetative propagation from bulb excised of Eleutherine sp. (Iridaceae) were cultured in Murashige and Skoog (MS) medium supplemented with plant growth regulator (PGR) Benzyl adenine (BA) 1 mg/l at initiation stage, BA (2 and 5) mg/l for induced shoot buds formation at multiplication stage. In this study also BA 2 mg/l and BA 2 mg/l combined with naphthalein acetic acid (NAA) 0.5 mg/l were treated for rooting planlets formation. Calli formation were induced with auxin PGR picloram 1 mg/l combined with 2,4 dichlorophenoxy acetic acid (2,4-D) 0.5 and 1 mg/l in concentrations. The media contained 2 mg/l cytokinin (BA) without auxin (NAA), resulted the highest shoot buds formation. Rooting planlets were produced in MS medium combined with BA and NAA.MS medium contains Picloram 1 mg/l and 2,4-D 1 mg/l was optimal for frequency oncalli initiation (100%) and the largest diameter of calli also represented in cultured MS medium with picloram 1 mg/l and 2,4-D 1mg/l. In acclimatization stage, 100% of planlets survived and successfully transplanted to soil medium in the field.Key words: in vitro, Eleutherine sp.

scholarly journals INFLUNCE OF GROWTH REGULATORS ON CALLUS INDUCTION OF CITRUS VOLKAMERIANA IN VITRO

2016 ◽  
Vol 47 (3) ◽  
Author(s):  
Al- Khazali & Hamad
Keyword(s):  
Acetic Acid ◽  
Callus Induction ◽  
Dry Weight ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Benzyl Adenine ◽  
College Of Agriculture ◽  
Citrus Volkameriana ◽  
Dichlorophenoxy Acetic Acid

This  research  was  conducted  in  the  plant  tissue  culture  Lab. College  of Agriculture / University  of  Baghdad  from  February to  October  2015. The aim  of  the  study  was  investigating  the  influences  of  combinations  of  Naphthalene  acetic  acid (NAA) , Thidiazuron (TDZ) Spermidine  (Spd. ) and 2,4-Dichlorophenoxy  acetic  acid (2,4-D) , Benzyl  adenine (BA) on callus  induction  and  adventitous  shoot  regeneration  originated  from  cotyledon  of  Citrus volkameriana  seeds. Seeds  were  disinfested  with 0.1 % of  HgCl2  for 15 minutes. The MS  medium  supplemented  with  (0.0,1.5 , 3.0 ) mg L-1  NAA in combination with (0.0, 0.05, 0.1) mg L-1  TDZ and (0.0, 0.5 ,1.0) mg L-1 Spd. and MS medium supplemented with (0.0, 1.5 , 3.0) mg L-1  2,4-D in combination with (0.0 ,1.0 , 2.0 )  mg L-1  BA and (0.0 ,0.5 , 1.0) mg L-1  Spd. the  interaction between 1.5 mg L-1  NAA and (0.05 , 0.1)  mg L-1  TDZ and the interaction between 3.0 mg L-1  NAA and (0.05 ,0.1) mg L-1  TDZ with all concentrations of Spd.   gave  the  highest  percentage of  callus  induction  100 % . While  the  MS  medium  supplemented  with  3 mg L-1 of  2,4-D in  combination  with  all  concentrations  of  BA  and  spd.  gave  the  highest  percentage 100 % of  callus induction. Results showed that  MS medium supplemented  with 1.5 mg L-1  NAA in combination  with  0.1 mg L-1 TDZ and  1.0 mg L-1 spd.  gave  the  highest  values  of  fresh  and  dry  weight  of  callus  (668.8, 44.59 ) mg  respectively . While  the  MS  medium  supplemented  with  3 mg L-1 2,4-D  in combination with 1.0 mg  L-1  spd.  And 0.0 mg L-1 BA gave  the  highest  values  of  fresh  and  dry  weight  of  callus  (709.2 , 47.28 ) mg  respectively.

scholarly journals Effect of 2,4-Dichlorophenoxy Acetic Acid and Activated Charcoal on Callus Induction of Cocos Nucifera L. Hybrid MATAG Inflorescence

2021 ◽  
Vol 12 (1S) ◽  
pp. 51-61
Author(s):  
Dhiya Dalila Zawawi ◽  
Mohd Fahmi Abu Bakar ◽  
Siti Nurkhalida Abd. Kadir
Keyword(s):  
Acetic Acid ◽  
Callus Induction ◽  
Activated Charcoal ◽  
Cocos Nucifera ◽  
High Demand ◽  
Immature Inflorescence ◽  
Cocos Nucifera L ◽  
Dichlorophenoxy Acetic Acid ◽  
Positive Effect

Cocos Nucifera Linn. Var. MATAG is a Dwarf coconut variety that had high demand in Malaysia but low supply. Vegetative propagation of high-yielding MATAG coconut by using in vitro cloning must be considered in contributing to increase coconut productivity. Thus, attempts were made to develop a protocol that would enhance callogenesis as a first preliminary step towards a protocol for mass propagation of C. nucifera L. var. MATAG. The anther isolated from immature inflorescence was used as explants and cultured on modified Eeuwens Y3 media in different concentrations of 2,4-dicholorophenoxy acetic acid (2,4-D) and activated charcoal. The highest callus induction percentage (31.25 ± 12.18) was observed in 20 mg/L 2,4-D. However, 2,4-D at any level tested were not statistically significant. Callus induction media supplemented with 0.5 mg/L activated charcoal gave the highest callusing percentage (25.89 ± 13.59 %) indicating a positive effect of activated charcoal on callusing even though the result obtained not significant compared to control (15.95 ± 6.76 %). But, activated charcoal supplemented in media produced a significant effect compared to control in reducing the percentage of browning. In conclusion, media supplemented with activated charcoal produced a higher rate on callus induction and preventing tissue browning in explant. Besides that, the anther and ovule explant may serve as an efficient explant to study the callus induction of C. nucifera L. var. MATAG and as a basis to screen the potential useful plant growth regulators for somatic embryogenesis.

Callus Induction and Plant Regeneration in Rauvolfia Serpentina (L.) Benth Ex. Kurz.

2009 ◽  
Vol 24 ◽  
pp. 82-88 ◽  
Author(s):  
Saraswoti Aryal ◽  
Sanu Devi Joshi
Keyword(s):  
Acetic Acid ◽  
Callus Induction ◽  
Coconut Milk ◽  
Ms Medium ◽  
Rauvolfia Serpentina ◽  
History Museum ◽  
Short Period ◽  
Murashige Skoog ◽  
Callus Tissue Culture

Rauvolfia serpentina (L.) ex. Kurz is an important medicinal plant. Callus induction and regeneration was studied from stem explant of in-vitro grown plant of Rauvolfia serpentina(L.) Benth. ex Kurz (Apocynaceae) on Murashige Skoog (1962) medium supplemented with 1mg/l 2,4-Dichlorophenocy acetic acid (2,4-D) and 1mg/l Kinetin (Kn). Vigorous growth of callus occurs after 4 weeks of culture. Callus was sub-cultured on Murashige and Skoog (MS) medium supplemented with different concentration of 2, 4-D (0.5-3.0 mg/l) and 10% coconut milk. Regeneration of plantlets occurred on MS medium containing 3 mg/1 of 2, 4-D and 10% coconut milk. These plantlets were rooted on MS medium supplemented with 1 mg/l IAA .The regenerated plantlets were able to grow on soil after short period ofacclimatization. Key words: Explant; In-vitro culture; MS medium;  2, 4 Dichlorophenoxy acetic acid; Kinetin; Callus; Tissue culture; Coconut milk. Journal of Natural History Museum Vol. 24, 2009 Page: 82-88

scholarly journals Influence of growth regulators and explants on shoot regeneration in carnation

2009 ◽  
Vol 36 (No. 4) ◽  
pp. 140-146 ◽  
Author(s):  
J.K. Kanwar ◽  
S. Kumar
Keyword(s):  
Acetic Acid ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Dianthus Caryophyllus ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Shoot Bud ◽  
Dichlorophenoxy Acetic Acid ◽  
Number Of Shoots

The influence of growth regulators, explants and their interactions on in vitro shoot bud formation from callus was studied in <I>Dianthus caryophyllus</I> L. The leaf and internode explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators. The highest callus induction was observed with 2 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 1 mg/l benzyl adenine (BA). Out of twenty seven shoot regeneration media tested, only 2 mg/l thidiazuron (TDZ) and zeatin alone or in combination with naphthalene acetic acid (NAA) and/or indole acetic acid (IAA) could differentiate calli. The highest average number of shoots was observed with 2 mg/l TDZ and 1 mg/l IAA. Significant differences were observed in calli producing shoots and number of shoots per callus in the explants of leaf and internode. The shoots were elongated and multiplied on MS medium supplemented with 1 mg/l BA and solidified with 1% agar. The shoots were rooted and hardened with 76% survival success in pots after six weeks of transfer to the pots.

Sign in / Sign up

Export Citation Format

Share Document