scholarly journals Isolation of Bacillus spp. Bacteria from Soil for Production of Cellulase

2019 ◽  
Vol 6 (1) ◽  
pp. 57-61 ◽  
Author(s):  
Amika Ahmed Manzum ◽  
Md Arafat Al Mamun
Keyword(s):  
Enzyme Activity ◽  
Genetic Improvement ◽  
Test Tube ◽  
Maximum Activity ◽  
Soil Samples ◽  
Shake Culture ◽  
Good Source ◽  
Tube Culture ◽  
Bacillus Spp ◽  
Food And Feed

Cellualse is one of the most important enzymes used in textile, detergent, paper, food and feed industries. Therefore, a study was undertaken to isolate Bacillus bacteria having the potential to produce cellulase from soil samples. 24 soil samples were analyzed and 54 presumptive Bacillus isolates were isolated after heating the soil samples at 80°C for 10 min. Among them 45 isolates showed enzyme activity ranging from 0.003 to 0.17 U/ml in test tubes containing 5 ml medium composed of (g/L) glucose 0.5 gm, peptone 0.75 gm, FeSO4 0.01 gm, KH2PO4 0.5 gm, and MgSO4 0.5 gm at 120 rpm, 37° C and pH 7. Among them 1RW, 2WS, 3YR, 4WT, 6 RR, and 9SS showed 0.17, 0.15, 0.14, 0.15, 0.147 and 0.14U/ml enzyme activities, respectively. Production of cellulase by these isolates was further scaled up to shake culture containing 50 ml medium similar to that used in test tube culture. Among the isolates 1 RW showed the maximum activity. This 1 RW was identified by API kit and showed that 59 % belongs to Bacillus licheniformis strain (51% confirmation) or Bacillus subtilis (31% confirmation). Further gene analysis is required to confirm the species. The genetic improvement study will make the isolate a good source of cellulase.

scholarly journals Partial purification and biochemical characterization of a new highly acidic NYSO laccase from Alcaligenes faecalis

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Soad A. Abdelgalil ◽  
Ahmad R. Attia ◽  
Reyed M. Reyed ◽  
Nadia A. Soliman
Keyword(s):  
Enzyme Activity ◽  
Sodium Dodecyl ◽  
Alcaligenes Faecalis ◽  
Maximum Activity ◽  
Sodium Oxalate ◽  
Partial Purification ◽  
Bromophenol Blue ◽  
Bromocresol Purple ◽  
Laccase Enzyme

Abstract Background Due to the multitude industrial applications of ligninolytic enzymes, their demands are increasing. Partial purification and intensive characterization of contemporary highly acidic laccase enzyme produced by an Egyptian local isolate designated Alcaligenes faecalis NYSO were studied in the present investigation. Results Alcaligenes faecalis NYSO laccase has been partially purified and intensively biochemically characterized. It was noticed that 40–60% ammonium sulfate saturation showed maximum activity. A protein band with an apparent molecular mass of ~ 50 kDa related to NYSO laccase was identified through SDS-PAGE and zymography. The partially purified enzyme exhibited maximum activity at 55 °C and pH suboptimal (2.5–5.0). Remarkable activation for enzyme activity was recognized after 10-min exposure to temperatures (T) 50, 60, and 70 °C; time elongation caused inactivation, where ~ 50% of activity was lost after a 7-h exposure to 60 °C. Some metal ions Cu2+, Zn2+, Co2+, Ni2+, Mn2+, Cd2+, Cr2+, and Mg2+ caused strong stimulation for enzyme activity, but Fe2+ and Hg2+ reduced the activity. One millimolar of chelating agents [ethylenediamine tetraacetic acid (EDTA), sodium citrate, and sodium oxalate] caused strong activation for enzyme activity. Sodium dodecyl sulfate (SDS), cysteine-HCl, dithiothreitol (DTT), β-mercaptoethanol, thioglycolic acid, and sodium azide caused strong inhibition for NYSO laccase activity even at low concentration. One millimolar of urea, imidazole, kojic acid, phenylmethylsulfonyl fluoride (PMSF), H2O2, and Triton X-100 caused activation. The partially purified NYSO laccase had decolorization activity towards different dyes such as congo red, crystal violet, methylene blue, fast green, basic fuchsin, bromophenol blue, malachite green, bromocresol purple eriochrome black T, and Coomassie Brilliant Blue R-250 with various degree of degradation. Also, it had a vast range of substrate specificity including lignin, but with high affinity towards p-anisidine. Conclusion The promising properties of the newly studied laccase enzyme from Alcaligenes faecalis NYSO strain would support several industries such as textile, food, and paper and open the possibility for commercial use in water treatment. It will also open the door to new applications due to its ligninolytic properties in the near future.

Phosphatase systems in Fasciolopsis buski Lankester, 1857 and Gastrodiscus aegyptiacus Cobbold, 1876

Parasitology ◽  
1973 ◽  
Vol 67 (2) ◽  
pp. 197-204 ◽  
Author(s):  
Madan M. Goil
Keyword(s):  
Enzyme Activity ◽  
Tartaric Acid ◽  
Maximum Activity ◽  
Nitrophenyl Phosphate ◽  
Biochemical Studies ◽  
Phosphate Hydrolysis ◽  
Fasciolopsis Buski ◽  
Enzyme I ◽  
Inhibited Enzyme ◽  
Acid Phosphomonoesterase

Biochemical studies on the non-specific phosphomonoesterases have demonstrated the presence of acid phosphomonoesterase with maximum activity at pH 4·0 in Gastrodiscus aegyptiacus (enzyme I) and at pH 4·5 in the case of Fasdolopsis buski (enzyme II). The Km for ρ-nitrophenyl phosphate hydrolysis was 0·66 mM for enzyme I and 1·1 mM for enzyme II. Different concentrations of fluoride, arsenate, tartrate, tartaric acid, cysteine and copper brought about inhibition of both enzymes and magnesium, iodoaeetate, iodoacetamide and EDTA had no influence on either enzyme activity. Cobalt activated both enzymes while zinc inhibited enzyme I and strongly stimulated enzyme II.

scholarly journals Parasitology and urban livestock farming in Nigeria : prevalence of ova in faecal and soil samples and animal ectoparasites in Makurdi

2007 ◽  
Vol 78 (1) ◽  
Author(s):  
E.A. Omudu ◽  
E.U. Amuta
Keyword(s):  
Test Tube ◽  
Soil Samples ◽  
Helminth Parasites ◽  
Livestock Farming ◽  
Chi Square ◽  
Zoonotic Infections ◽  
Faecal Samples ◽  
Significant Difference ◽  
Flotation Technique ◽  
Chi Square Analysis

Domestic environmental pollution resulting from urban livestock farming was investigated in Makurdi using parasitological techniques. The test tube flotation technique was used for the parasitological analysis of animal faecal matter and soil samples collected from residential premises. Ectoparasitic fauna of dogs, goats, sheep and cattle cohabiting with humans within the same residential compound were also collected and identified. The hand-picking and body brushing methods were employed to search for ticks, fleas, lice and mites. Of the 150 soil samples examined, 55 (36.7 %) were positive for 1 or more eggs of helminth parasites. There was no significant difference in the distribution of eggs in the soil samples from the 3 areas sampled (c2=0.046, df=2, P>0.05). Ascaris species were the dominant parasite eggs found. Of the 180 faecal samples examined, 107 (59.4 %) were positive for 1 or more eggs of helminth parasites. Chi-square analysis showed no significant difference in the level of infection of different animal faeces sampled (c2=5.74, df=4, P>0.05). Ascaris species were again the dominating helminth parasite eggs found. There was also no significant difference in the prevalence of helminth eggs in the animal faecal samples from the 3 areas sampled (c2=5.99, df=4, P>0.05). A total of 1908 ectoparasites was recovered (ticks: 32.80 %; lice: 22.43 %; fleas: 22.06% and mite: 22.69 %). There was no significant difference in infestation animals between sexes (c2=0.10, df=4, P>0.05). The predominant genus encountered for ticks were Amblyomma, while Linognathus (43.90%), Ctenocephalides (97.38%) and Sarcoptes (58.89 %) were most predominant for lice, fleas and mites respectively. The public health implications of the findings, especially as these relate to the increasing incidence and prevalence of zoonotic infections, are discussed.

scholarly journals Isolation and characterization of natural protease producers of Bacillus spp. from Soil samples

2018 ◽  
Author(s):  
Ylberina Baliu ◽  
Bahtir Hyseni ◽  
Shkëlqim Hyseni ◽  
Aida Rushiti ◽  
Flora Ferati ◽  
...  
Keyword(s):  
Soil Samples ◽  
Isolation And Characterization ◽  
Bacillus Spp

scholarly journals Clay dispersion and loss in Oxisol treated with different concentrations of limestone

2017 ◽  
Vol 38 (6) ◽  
pp. 3907 ◽  
Author(s):  
Wesley Machado ◽  
Thadeu Rodrigues de Melo ◽  
João Tavares Filho
Keyword(s):  
Soil Ph ◽  
Dispersion Analysis ◽  
Test Tube ◽  
Soil Samples ◽  
Base Saturation ◽  
Clay Dispersion ◽  
Clay Concentration ◽  
Significant Change

The addition of limestone to the soil may cause clay dispersion and loss. In this study, we aimed to estimate clay dispersion and loss in Oxisol incubated with different concentrations of limestone. To this end, soil samples were collected from a depth of 0.0-0.20 m, treated with limestone at a concentration of 1.46 Mg ha-1 or 2.73 Mg ha-1 to raise base saturation to 60% and 70%, respectively, and incubated for 12 months under greenhouse conditions. Clay dispersion analysis was conducted monthly using the test tube method. The results showed that clay dispersion increased with soil pH and time after limestone application. However, no significant change in clay concentration was identified in the control (no limestone application). At 60% base saturation, clay loss was first observed to be significantly higher than that of the control at 4 months after application and reached a maximum at 9 months after application. At 70% base saturation, clay loss was also first observed to be significantly higher than that of the control at 4 months after application and reached a maximum at 6 months after application. Overall, our data revealed that clay dispersion due to limestone application was approximately 16 g kg-1 over a period of 12 months and increased with the increasing concentrations of Ca and Mg.

scholarly journals Enzymatic activity measured by microcalorimetry in soil amended with organic residues

2011 ◽  
Vol 35 (4) ◽  
pp. 1167-1178 ◽  
Author(s):  
Karina Cenciani ◽  
Sueli dos Santos Freitas ◽  
Silvana Auxiliadora Missola Critter ◽  
Claudio Airoldi
Keyword(s):  
Enzyme Activity ◽  
Enzymatic Activity ◽  
Thermal Effect ◽  
Soil Samples ◽  
Cattle Manure ◽  
Control Treatment ◽  
Municipal Sewage Sludge ◽  
Organic Residues ◽  
Earthworm Casts ◽  
Commercial Enzymes

Enzymatic activity is an important property for soil quality evaluation. Two sequences of experiments were carried out in order to evaluate the enzymatic activity in a soil (Rhodic Eutrudox) amended with cattle manure, earthworm casts, or sewage sludges from the municipalities of Barueri and Franca. The activity of commercial enzymes was measured by microcalorimetry in the same soil samples after sterilization. In the first experiment, the enzyme activities of cellulase, protease, and urease were determined in the soil samples during a three month period. In the second sequence of experiments, the thermal effect of the commercial enzymes cellulase, protease, and urease on sterilized soil samples under the same tretaments was monitored for a period of 46 days. The experimental design was randomized and arranged as factorial scheme in five treatments x seven samplings with five replications. The treatment effects were statistically evaluated by one-way analysis of variance. Tukey´s test was used to compare means at p < 0.05. The presence of different sources of organic residues increased the enzymatic activity in the sampling period. Cattle manure induced the highest enzymatic activity, followed by municipal sewage sludge, whereas earthworm casts induced the lowest activity, but differed from control treatment. The thermal effect on the enzyme activity of commercial cellulase, protease, and urease showed a variety of time peaks. These values probably oscillated due to soil physical-chemical factors affecting the enzyme activity on the residues.

Development of Isocitric Lyase Activity in Germinating Prickly Sida Seed

Weed Science ◽  
1976 ◽  
Vol 24 (3) ◽  
pp. 298-301 ◽  
Author(s):  
Bonnie J. Reger ◽  
E. Wayne Smith
Keyword(s):  
Enzyme Activity ◽  
Actinomycin D ◽  
Maximum Activity ◽  
Lyase Activity ◽  
Radicle Protrusion ◽  
Sida Spinosa ◽  
D 12 ◽  
Prickly Sida

Maximum activity of isocitric lyase (EC 4.1.3.1) was reached at 24 hr incubation of afterripened (nondormant) prickly sida (Sida spinosaL.) seed. Enzyme activity declined gradually with incubation times in excess of 24 hr. Actinomycin D (Act D) at 5 μg/ml had essentially no effect on 24-hr germination but inhibited development of isocitric lyase activity 83%. Cycloheximide (CH) at 10 μg/ml inhibited 24-hr germination of punctured seed only 7% but inhibited development of isocitric lyase activity 76%. Seed incubated in water 12 hr before being transferred to Act D at 5 μg/ml for an additional 12 hr did not escape sensitivity to the antibiotic. Isocitric lyase activity was inhibited when assayed at 24 hr total incubation. In the reverse experiment, seed incubated in Act D 12 hr before being transferred to water, isocitric lyase activity at 24 hr was not affected. Apparently prickly sida seed lacked a performed mRNA for isocitric lyase and transcription and translation of the mRNA occurred shortly after initiation of radicle protrusion (~8 hr).

scholarly journals Purification of 2-oxoaldehyde dehydrogenase and its dependence on unusual amines

1975 ◽  
Vol 149 (3) ◽  
pp. 609-617 ◽  
Author(s):  
J Dunkerton ◽  
S P James
Keyword(s):  
Enzyme Activity ◽  
Gel Electrophoresis ◽  
General Structure ◽  
Polyacrylamide Gel Electrophoresis ◽  
Maximum Activity ◽  
Polyacrylamide Gel ◽  
Ph Optimum ◽  
Ph Values ◽  
Sheep Liver ◽  
Living Tissues

1. 2-Oxoaldehyde dehydrogenase was purified from sheep liver and gave one band on polyacrylamide-gel electrophoresis. 2. The enzyme was completely dependent for its activity on the presence of Tris or one of a number of related amines, all of general structure: (See article). When more than one R group was hydrogen no enzyme activity was observed. 3. Only one of these amines is known to exist in living tissues and large concentrations of all amines were required for maximum activity. L-2-Aminopropan-1-ol was the most effective amine on the basis of substrate Km and Vmax. values and the amine Km values. 4. The enzyme was activated by phosphate which lowered the Km values for methylglyoxal, amine and NAD+. 5. The pH optimum of the enzyme was 9.3 and there was no activity at pH values below 7.8. A search for activators that might produce activity at pH 7.4 proved unsuccessful. 6. The enzyme was inhibited by rather large concentrations of barbiturates (6-46 mM) and nitro-alcohol analogues of the activating amines (66-139 mM).

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