scholarly journals Characteristics and phylogeny of DREB gene subfamily in soybeans [Glycine max (L.) Meril]

2021 ◽  
Vol 63 (2) ◽  
pp. 60-64
Author(s):  
Thi Ngoc Lan Nguyen ◽  
◽  
Phutthakone Vaciaxa ◽  
Thanh Chung Nguyen ◽  
Huu Quan Nguyen ◽  
...  
Keyword(s):  
Glycine Max ◽  
Promoter Region ◽  
Amino Acid Sequences ◽  
Cis Acting ◽  
Comprehensive Information ◽  
Glycine Max L ◽  
Responsive Element ◽  
Relationship Of ◽  
Ap2 Domain ◽  
Dreb Gene

Dehydration responsive element binding proteins (DREB) are transcription factors linked to cis-acting elements of the promoter region, which regulate plant gene expression in response to abiotic stress. In this study, 69 DREB gene sequences of soybean from NCBI belonging to 18 GmDREB (Glycine max DREB) genes of 1 to 8 copies distributed on 17 chromosomes were identified in which GmDREB3 has 8 copies and the rest consisted of 1 to 4 ones. The motif PTPEMAARAYDVAALALKGPSARLNFPEL containing 11 points associated with the promoter of functional genes existed in 4 main types with the most popular one RGRRWKERRWT found in 13/18 DREB proteins was regarded as the popular AP2 domain of DREB protein. The phylogenetic tree based on the nucleotide sequences of GmDREB genes and the amino acid sequences of the AP2 domain expresses the evolution and relationship of the DREB subfamily in soybean. This study provides comprehensive information about the DREB subfamily, which formed the basis for experimental analyses to clarify the function of some members of this subfamily.

The harvest-responsive region of the Asparagus officinalis sparagine synthetase promoter reveals complexity in the regulation of the harvest response

2008 ◽  
Vol 35 (12) ◽  
pp. 1212 ◽  
Author(s):  
Donald A. Hunter ◽  
Lyn M. Watson
Keyword(s):  
Promoter Region ◽  
Constitutive Expression ◽  
Wound Response ◽  
Asparagus Officinalis ◽  
Minimal Promoter ◽  
Nucleotide Substitutions ◽  
Cis Acting ◽  
Gus Reporter ◽  
Highly Active ◽  
Responsive Element

The activity of a 1915-bp asparagine synthetase (AS) promoter of Asparagus officinalis L. was induced in mature leaves of transgenic Arabidopsis thaliana (L.) Heynh. plants when the leaves were detached and held in water for 24 h. To understand this induction by harvest, variants of the AS promoter were linked to the β-glucuronidase GUS reporter gene. Harvest induction in the leaves required detachment and was not simply a wound response. Two regions in the AS promoter (Region A, –640 to –523; Region B, –524 to –383) were independently able to confer harvest response to the otherwise unresponsive –383AS (minimal) promoter. Region A was studied in further detail. Various truncations, deletions, or nucleotide substitutions of Region A affected activity and fold induction of the minimal promoter. However, no harvest-inducible cis-acting element within Region A was identified. Although the minimal promoter contained a dehydration-responsive element and ACGT elements similar to ABA-responsive regulatory motifs these were not needed by the upstream regulatory regions for directing harvest response. When four copies of Region A were linked to the minimal promoter it became highly active in leaves before harvest. Deletions within Region A showed that it required its complete 117 bp for driving harvest response, yet the region cannot simply be thought of as a harvest-responsive module, since its concatemerisation led to constitutive expression.

scholarly journals The Relationship of the Contents of Calcium, Potassium and Pectin with Cracking of Seed Coat of Soybean (Glycine max (L.) Merr.)

2011 ◽  
Vol 80 (4) ◽  
pp. 433-440
Author(s):  
Takayo Saikusa ◽  
Shin-ichiro Kawase ◽  
Toshiroh Horino ◽  
Kyouko Toda ◽  
Yoshiyuki Nakamura
Keyword(s):  
Glycine Max ◽  
Seed Coat ◽  
Glycine Max L ◽  
Relationship Of ◽  
The Relationship

scholarly journals QTL Analysis of Resistance to High-Intensity UV-B Irradiation in Soybean (Glycine max [L.] Merr.)

2019 ◽  
Vol 20 (13) ◽  
pp. 3287 ◽  
Author(s):  
Min Young Yoon ◽  
Moon Young Kim ◽  
Jungmin Ha ◽  
Taeyoung Lee ◽  
Kyung Do Kim ◽  
...  
Keyword(s):  
Glycine Max ◽  
High Intensity ◽  
Crop Yields ◽  
Light Exposure ◽  
Amino Acid Sequences ◽  
Ultraviolet B ◽  
Leaf Damage ◽  
Biotic And Abiotic Stress ◽  
Ril Population ◽  
Glycine Max L

High-intensity ultraviolet-B (UV-B) irradiation is a complex abiotic stressor resulting in excessive light exposure, heat, and dehydration, thereby affecting crop yields. In the present study, we identified quantitative trait loci (QTLs) for resistance to high-intensity UV-B irradiation in soybean (Glycine max [L.]). We used a genotyping-by-sequencing approach using an F6 recombinant inbred line (RIL) population derived from a cross between Cheongja 3 (UV-B sensitive) and Buseok (UV-B resistant). We evaluated the degree of leaf damage by high-intensity UV-B radiation in the RIL population and identified four QTLs, UVBR12-1, 6-1, 10-1, and 14-1, for UV-B stress resistance, together explaining 20% of the observed phenotypic variation. The genomic regions containing UVBR12-1 and UVBR6-1 and their syntenic blocks included other known biotic and abiotic stress-related QTLs. The QTL with the highest logarithm of odds (LOD) score of 3.76 was UVBR12-1 on Chromosome 12, containing two genes encoding spectrin beta chain, brain (SPTBN, Glyma.12g088600) and bZIP transcription factor21/TGACG motif-binding 9 (bZIP TF21/TGA9, Glyma.12g088700). Their amino acid sequences did not differ between the mapping parents, but both genes were significantly upregulated by UV-B stress in Buseok but not in Cheongja 3. Among five genes in UVBR6-1 on Chromosome 6, Glyma.06g319700 (encoding a leucine-rich repeat family protein) had two nonsynonymous single nucleotide polymorphisms differentiating the parental lines. Our findings offer powerful genetic resources for efficient and precise breeding programs aimed at developing resistant soybean cultivars to multiple stresses. Furthermore, functional validation of the candidate genes will improve our understanding of UV-B stress defense mechanisms.

scholarly journals AVALIAÇÃO DO NÍVEL RESIDUAL TEÓRICO E EXPERIMENTAL DE ENDOSULFAN EM ÓLEO DE SOJA (Glycine max. (L) Merrill)

2001 ◽  
Vol 11 ◽  
Author(s):  
CÉLIA MARIA DIAS CORRÊA ◽  
JORGE JOSÉ DO VALE OLIVEIRA ◽  
VALDEMAR LUIZ TORNISIELO
Keyword(s):  
Glycine Max ◽  
Soybean Oil ◽  
Integrated Management ◽  
Insecticide Concentration ◽  
Glycine Max L ◽  
General Average ◽  
Para Determinar ◽  
Experimental Values ◽  
Conventional Systems ◽  
Relationship Of

Visou-se quantificar teórica e experimentalmente os níveis residuais de Endosulfan e seus principais metabólitos em óleo de soja e avaliar a confiabilidade dos valores teóricos em relação ao experimental. A soja foi cultivada no campo nos sistemas manejo integrado de pragas (MIP) e convencional. Extraiu-se o óleo de soja utilizando o solvente hexano em extrator Soxhlet. Determinaram-se as concentrações do Endosulfan no grão e no óleo, mediante cromatografia em fase gasosa, com detector de captura de elétrons (Ni63). A concentração de Endosulfan no óleo foi obtida teoricamente multiplicando-se a relação entre a massa grão/óleo pela concentração deste inseticida encontrada experimentalmente no grão. A relação percentual da concentração de Endosulfan no óleo, entre os valores teórico/experimental, enquadraram-se na faixa de 94% a 114% e 81% a 92%, respectivamente, para os sistemas MIP e convencional. Como a média geral dos níveis de Endosulfan, calculada teoricamente, alcançou 94% do valor obtido experimentalmente concluiu-se que o cálculo teórico pode ser utilizado para determinar os níveis deste inseticida no óleo de soja. EVALUATION OF THE THEORETICAL AND EXPERIMENTAL RESIDUAL LEVELS OF ENDOSULFAN IN SOYBEAN (Glycine max. (L) Merrill ) OIL Abstract The objective of this study was to quantify theoretically and experimentally the residual levels of Endosulfan and main metabolites in soybean oil and to evaluate the reliability of the theoretical values in relation to the experimental values. The soybean was grown in the fields in the pest integrated management (PIM) and conventional systems. The soybean oil was extracted by using hexane as solvent in Soxhlet extractor. The Endosulfan concentrations were determined in the grains and oil by means of gas chromatography equipped with electron capture detector (Ni63). The Endosulfan concentration in soybean oil was theoretically obtained by multiplying the relationship between the grain mass/ soybean oil by this insecticide concentration determined experimentally in the grain. The percents relationship of the concentration of Endosulfan in the soybean oil between the theoretical/experimental values were in the range of 94% to 119% and 81% to 92%, respectively, for the PIM and conventional system. As the general average levels of Endosulfan, theoretically calculated, reached 94% of the value obtained experimentally it was concluded that the theoretical calculation could be used for determination this insecticide levels in the soybean oil.

Prevalence of the pit and antipit in the genus Glycine

1987 ◽  
Vol 45 ◽  
pp. 986-987
Author(s):  
R. W. Yaklich ◽  
E. L. Vigil ◽  
W. P. Wergin
Keyword(s):  
Amino Acids ◽  
Endoplasmic Reticulum ◽  
Glycine Max ◽  
Seed Coat ◽  
Cell Types ◽  
Abaxial Surface ◽  
Legume Seed ◽  
Initial Report ◽  
Cone Cells ◽  
Glycine Max L

The legume seed coat is the site of sucrose unloading and the metabolism of imported ureides and synthesis of amino acids for the developing embryo. The cell types directly responsible for these functions in the seed coat are not known. We recently described a convex layer of tissue on the inside surface of the soybean (Glycine max L. Merr.) seed coat that was termed “antipit” because it was in direct opposition to the concave pit on the abaxial surface of the cotyledon. Cone cells of the antipit contained numerous hypertrophied Golgi apparatus and laminated rough endoplasmic reticulum common to actively secreting cells. The initial report by Dzikowski (1936) described the morphology of the pit and antipit in G. max and found these structures in only 68 of the 169 seed accessions examined.

PENGARUH PEMBERIAN PUPUK ORGANIK CAIR ASAL LIMBAH TERHADAP SERAPAN HARA SERTA PRODUKSI TANAMAN KEDELAI DI LAHAN GAMBUT

2017 ◽  
Vol 2 (02) ◽  
pp. 204-218
Author(s):  
Hendra Saputra ◽  
Intan Sari ◽  
Muhammad Arfah
Keyword(s):  
Glycine Max ◽  
Glycine Max L

Penelitian tentang pengaruh pemberian Pupuk organik cair (POC) asal limbah tumbuhan terhadap serapan hara N dan P serta produksi tanaman kedelai (Glycine max (L) Merrill) di lahan gambut telah dilaksanakan di kampus II Unisi Fakultas Pertanian Jl. Lintas Propinsi Parit 01, Desa Pulau Palas, Kecamatan Tembilahan Hulu, Kabupaten Indragiri Hilir Propinsi Riau. Dimulai dari bulan Agustus sampai bulan Oktober 2013. Tujuan dari penelitian ini untuk mendapatkan POC asal limbah tumbuhan yang terbaik untuk serapan hara N dan P serta produksi tanaman kedelai di lahan gambut. Penelitian ini menggunakan rancangan acak kelompok (RAK) faktor tunggal dengan 7 perlakuan dan 4 ulangan, 2 tanaman dijadikan sampel. Perlakuan dosis POC limbah tanaman pisang dan POC limbah sayur kol yang diberikan yaitu 0 L/Ha, 200 L/Ha, 400 L/Ha dan 600 L/Ha. Parameter pengamatan yaitu : serapan hara N dan P pada fase awal generatif, tinggi tanaman, jumlah bintil akar, polong hampa, produksi perplot, berat 100 biji dan brangkasan kering. Data pengamatan dianalisis dengan sidik ragam (ANOVA) dan dilanjutkan dengan Uji Lanjut Tukey HSD pada taraf 5%. Berdasarkan penelitian yang telah dilaksanakan dapat disimpulkan bahwa pemberian POC asal limbah tumbuhan tidak berpengaruh nyata terhadap serapan hara N dan P, tinggi tanaman, jumlah bintil akar, polong hampa, brangkasan kering tetapi berpengaruh nyata terhadap produksi perplot dan berat 100 biji.

PERTUMBUHAN DAN HASIL SEMBILAN KULTIVAR KEDELAI (Glycine max (L.) Merrrill) DI LAHAN SAWAH

2018 ◽  
Vol 1 (2) ◽  
pp. 96
Author(s):  
Siti Wahyuni ◽  
Umi Trisnaningsih ◽  
Meilina Prasetyo
Keyword(s):  
Glycine Max ◽  
Glycine Max L

COPPER AND ZINC INDUCED CHANGES IN SOYBEAN (Glycine max (L.) Merr.)

1970 ◽  
pp. 09
Author(s):  
K. SANKAR GANESH ◽  
P. SUNDARAMOORTHY
Keyword(s):  
Heavy Metals ◽  
Glycine Max ◽  
Aquatic Environment ◽  
Morphological Traits ◽  
Industrial Activities ◽  
Copper And Zinc ◽  
Glycine Max L ◽  
Zinc Concentrations ◽  
Induced Changes

Heavy metals are one of the most important pollutants released to the aquatic environment by the various industrial activities. The use of these wastewater for irrigation results accumulation of heavy metals in soil and plants. So, the present investigation deals with the various concentrations (0, 5, 10, 25, 50, 100, 200 and 300 mg/l) of copper and zinc on germination studies of soybean. The different concentrations of copper and zinc were used for germination studies. The seedlings were allowed to grow upto seven days. The studied morphological traits increased at 5 mg/l concentration and these parameters are gradually decreased with the increase of copper and zinc concentrations.

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