scholarly journals Anti-inflammatory Effects of 6,6′-bieckol and 6,8′-bieckol from Eisenia arborea on Mouse Ear Swelling

2017 ◽  
Vol 23 (3) ◽  
pp. 475-480 ◽  
Author(s):  
Yoshimasa Sugiura ◽  
Masakatsu Usui ◽  
Hirotaka Katsuzaki ◽  
Kunio Imai ◽  
Masaaki Miyata
Keyword(s):  
Eisenia Arborea ◽  
Mouse Ear ◽  
Anti Inflammatory

The anti-inflammatory effects of phlorotannins from Eisenia arborea on mouse ear edema by inflammatory inducers

2013 ◽  
Vol 5 (4) ◽  
pp. 2019-2023 ◽  
Author(s):  
Yoshimasa Sugiura ◽  
Ryusuke Tanaka ◽  
Hirotaka Katsuzaki ◽  
Kunio Imai ◽  
Teruo Matsushita
Keyword(s):  
Ear Edema ◽  
Eisenia Arborea ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Mouse Ear Edema

Ketoconazole-p Aminobenzoic Cocrystal Exhibits a Potent Anti-inflammatory Effect on the Skin of BALBc Mice 

2021 ◽  
Author(s):  
Sorina Danescu ◽  
Gabriela Adriana Filip ◽  
Remus Moldovan ◽  
Diana Olteanu ◽  
Andras Nagy ◽  
...  
Keyword(s):  
Fungal Infections ◽  
Parent Drug ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Nfκb Pathway ◽  
Cutaneous Mycosis ◽  
Anti Inflammatory Cytokine ◽  
Global Health Problem ◽  
Inflammatory Action ◽  
Inflammatory Effect

Abstract Fungal infections are a growing global health problem. Therefore, our group has designed and characterized a novel cocrystal formulation starting from ketoconazole and para-amino benzoic acid, named KET-PABA aiming to improve the bioavailability, biocompatibility, and efficiency of the parent drug. The cocrystal showed improved physical properties, such as stability in suspension, solubility, as well as antimycotic efficiency as compared to ketoconazole. The current study investigated the local possible side effects induced on BALBc mice skin by the application of KET-PABA cocrystal. KET-PABA proved to be safe, without signs of skin sensitization as shown by the mouse ear sensitization test (MEST), or histopathology. KET-PABA induced a potent anti-inflammatory effect through the inhibition of proinflammatory cytokines such as IL1α, IL1β, IL6 and TNFα, and other inflammation promoters such as NRF2, compared to the vehicle. KET-PABA had no effect on the levels of the anti-inflammatory cytokine IL10, or proinflammatory enzyme COX2 and had minimal effects on the activation of the NFκB pathway. Overall, KET-PABA application induced no sensitization, moreover, it induced an anti-inflammatory response. Based on the improved antimycotic effect versus ketoconazole and the anti-inflammatory action, KET-PABA cocrystal has the potential to be an efficient drug in the treatment of cutaneous mycosis.

Antifungal, Anti-Inflammatory and Cytotoxic Effects of Zingiber cassumunar Gel

2018 ◽  
Vol 773 ◽  
pp. 360-364 ◽  
Author(s):  
Sroisiri Thaweboon ◽  
Boonyanit Thaweboon ◽  
Rattiporn Kaypetch
Keyword(s):  
Cell Culture ◽  
Antifungal Activity ◽  
Diffusion Method ◽  
Cytotoxic Effects ◽  
Ear Edema ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Zingiber Cassumunar ◽  
Mouse Ear Edema ◽  
Inflammatory Effect

This study aimed to investigate the antifungal, anti-inflammatory and cytotoxic effects of Zingiber cassumunar gel. The gel was prepared from essential oil of Zingiber cassumunar rhizome by the Thailand Institute of Scientific and Technological Research. Antifungal activity of the gel was firstly determined by the well diffusion method against Candida albicans ATCC 10238 and candida strain isolated from the patient’s lesion. Then, the Agar overlay technique was used to test the cytotoxicity of Z. cassumunar gel on mouse fibroblasts (ATCC clone 929) according to ISO 7405. For anti-inflammatory effect of the gel, TPA (carrageenan lambda type IV, 12-O-tetradecanoylphorbol-13- acetate)-induced mouse ear edema method was used. The results of well diffusion showed that Z. cassumunar gel was quite a potent antifungal agent against both strains of tested C. albicans with inhibition zones of 12-13 mm. In the cytotoxicity test, the gel exhibited no toxicity to cell culture. In addition, topical administration of Z. cassumunar gel could decrease mouse ear edema induced by TPA. At 30 and 60 min-time points, Z. cassumunar gel showed higher anti-inflammatory activity than triamcinolone which was used as reference anti-inflammatory drug. In conclusion, gel prepared from Z. cassumunar oil showed antifungal activity against both strains of C. albicans. In addition, its anti-inflammatory effect was demonstrated within 30 min by the TPA-induced mouse ear edema model. The gel was non-toxic to cell culture after 24-h incubation. Further studies are needed to clarify the safety and benefit of this gel for clinical use in the treatment of candidal infection and inflammation.

scholarly journals Anti-Helicobacter pylori and Anti-Inflammatory Effects and Constituent Analysis of Modified Xiaochaihutang for the Treatment of Chronic Gastritis and Gastric Ulcer

2018 ◽  
Vol 2018 ◽  
pp. 1-15 ◽  
Author(s):  
Xin Chen ◽  
Lijuan Hu ◽  
Huanhuan Wu ◽  
Wei Liu ◽  
Shuhe Chen ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Ulcer ◽  
Chronic Gastritis ◽  
Gastric Ulcers ◽  
Etoac Extract ◽  
Rat Paw Edema ◽  
Edema Model ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Rat Paw

Chronic gastritis and gastric ulcers are prevalent throughout the world and are considered to be a global health problem. Modified Xiaochaihutang (MXCHT) prescription is broadly used in traditional medicine hospital for the treatment of gastritis. In order to assess the anti-Helicobacter pylori (H. pylori) effect of MXCHT, agar diffusion method in vitro and fluid dilution method for the minimal inhibitory concentration (MIC) were established. The anti-inflammatory effects were then evaluated using mouse ear edema model and rat paw edema model. The ethanol-induced gastric ulcer method was employed to verify the gastroprotective effect of active extracts in MXCHT. HPLC-TOF-MS/MS was used for analyzing the possible active constituents after oral administration of effective extracts in ethanol-induced gastric ulcer models. MXCHT and 4 different extracts of the bacterial inhibition diameter and MIC were dramatically decreased compared with control group, showing anti-Helicobacter pylori effects. High dose groups of MXCHT, water extract, EtOAc extract, and n-BuOH extract displayed significant anti-inflammatory effects in xylene-induced mouse ear edema model and carrageenan-induced rat paw edema model test. MXCHT and all active extracts exhibited gastroprotective activity and prevented gastric lesions induced by ethanol in rats. 4 prototype components and 4 metabolites were identified after oral administration of EtOAc extract. In addition, 6 prototype components and 6 metabolites were identified in n-BuOH extract. MXCHT, EtOAc extract, and n-BuOH extract demonstrate gastroprotective effects through anti-Helicobacter pylori and anti-inflammatory activities. Thus, this prescription may be a suitable natural source for the prevention and treatment of chronic gastritis and gastric ulcers.

scholarly journals Anti-Inflammatory Activity of Cnidoscolus aconitifolius (Mill.) Ethyl Acetate Extract on Croton Oil-Induced Mouse Ear Edema

2021 ◽  
Vol 11 (20) ◽  
pp. 9697
Author(s):  
Eduardo Padilla-Camberos ◽  
Omar Ricardo Torres-Gonzalez ◽  
Ivan Moises Sanchez-Hernandez ◽  
Nestor Emmanuel Diaz-Martinez ◽  
Oscar Rene Hernandez-Perez ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Inflammatory Activity ◽  
Therapeutic Effects ◽  
Ear Edema ◽  
Croton Oil ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Mouse Ear Edema

Nowadays, there is a growing interest in the development of medicinal plant-based therapies to diminish the ravages of the inflammatory process related to diseases and tissue damage. Most therapeutic effects of these traditional medicinal plants are owed to their phenolic and antioxidant properties. C. aconitifolius is a traditional medicinal plant in Mexico. Previous characterization reports have stated its high nutritional and antioxidant components. The present study aimed to better understand the biological activity of C. aconitifolius in inflammation response. We developed an ethyl acetate extract of this plant to evaluate its anti-inflammatory capacity and its flavonoid content. The topical anti-inflammatory effect of the ethyl acetate extract of C. aconitifolius was determined by the croton oil-induced mouse ear edema test, while flavonoid detection and concentration were determined by thin layer chromatography and the aluminum chloride colorimetric assay, respectively. Topical application of the extract showed significant inhibition of the induced-ear edema (23.52 and 49.41% for 25 and 50 mg/kg dose, respectively). The extract also exhibited the presence of flavonoids. The finding of the anti-inflammatory activity exerted by the C. aconitifolius and the identification of its active principles may suggest and support its use for inflammation treatment.

scholarly journals Ethanolic Extract of Origanum vulgare Suppresses Propionibacterium acnes-Induced Inflammatory Responses in Human Monocyte and Mouse Ear Edema Models

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 1987 ◽  
Author(s):  
Lu-Te Chuang ◽  
Tsung-Hsien Tsai ◽  
Tsung-Jung Lien ◽  
Wen-Cheng Huang ◽  
Jun-Jen Liu ◽  
...  
Keyword(s):  
Propionibacterium Acnes ◽  
Inflammatory Responses ◽  
Acne Vulgaris ◽  
Suppressive Effect ◽  
Transcriptional Level ◽  
Origanum Vulgare ◽  
Ear Edema ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Mouse Ear Edema

Acne vulgaris (acne) is a common inflammatory skin disorder, and Propionibacterium acnes plays a major role in the development and progression of acne inflammation. Herbs possessing antimicrobial and anti-inflammatory activity have been applied as a medical option for centuries. In this study, we examined the suppressive effect of ethanolic oregano (Origanum vulgare) extract on live P. acnes-induced in vivo and in vitro inflammation. Following ethanol extraction of oregano leaves, four compounds with strong antioxidant activity, including rosmarinic acid, quercetin, apigenin, and carvacrol, were identified by high-performance liquid chromatography. Using the mouse ear edema model, we demonstrated that ethanol oregano extracts (EOE) significantly suppressed P. acnes-induced skin inflammation, as measured by ear thickness (32%) and biopsy weight (37%). In a separate study, using the co-culture of P. acnes and human THP-1 monocytes, EOE reduced the production of interleukin (IL)-8, IL-1β and tumor necrosis factor (TNF)-α up to 40%, 37%, and 18%, respectively, as well as the expression of these three pro-inflammatory mediators at the transcriptional level. Furthermore, EOE inhibited the translocation of nuclear factor-kappa B (NF-κB) into the nucleus possibly by inactivating toll-like receptor-2 (TLR2). The suppressive effect of EOE on live P. acnes-induced inflammatory responses could be due, in part, to the anti-inflammatory and antioxidant properties, but not the anti-microbial effect of EOE.

SPASMOLYTIC, ANTI-INFLAMMATORY, AND ANTIOXIDANT ACTIVITY OF SALVIA GESNERIFLORA LINDLEY

2018 ◽  
Vol 15 (4) ◽  
pp. 72
Author(s):  
Abraham Gómez-Rivera ◽  
Manasés González-Cortazar ◽  
Ammy Joana Gallegos-García ◽  
Armando Escobar-Ramos ◽  
Gabriel Flores-Franco ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Guinea Pig ◽  
Ursolic Acid ◽  
Antioxidant Potential ◽  
Guinea Pig Ileum ◽  
Chromatographic Profile ◽  
Mouse Ear ◽  
Inflammatory Edema ◽  
Anti Inflammatory

Background: Salvia gesneriflora Lindley is employed in traditional Mexican medicine for the treatment of several diseases. Materials and Methods: The chromatographic profile of three extracts (SgH, SgD and SgM) of this plant allowed the identification of 11 components in SgH, the presence of rosmarinic (1), chlorogenic (2) and caffeic (3) acids and quercetin glucoside (4) in SgM and of ursolic acid (5) in SgD. The spasmolytic (electrically induced contractions of guinea-pig ileum), anti-inflammatory (edema in mouse ear) and antioxidant potential (DPPH, ABTS and FRAP assays) of the extracts were evaluated. Results: SgM showed the highest percentage of relaxation (80.67 ± 1.633%) with no significant difference (p

Anti-Inflammatory Effect of Sargassum patens C. Agardh Ethanol Extract in LPS-induced RAW264.7 Cells and Mouse Ear Edema

2017 ◽  
Vol 45 (2) ◽  
pp. 110-117 ◽  
Author(s):  
Min-Ji Kim ◽  
Min-Ju Kim ◽  
Koth Bong Woo Ri Kim ◽  
Sun Hee Park ◽  
Hyeun Deok Choi ◽  
...  
Keyword(s):  
Ethanol Extract ◽  
Raw264.7 Cells ◽  
Ear Edema ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Sargassum Patens ◽  
Mouse Ear Edema ◽  
Inflammatory Effect

scholarly journals Retrofractamide C Derived from Piper longum Alleviates Xylene-Induced Mouse Ear Edema and Inhibits Phosphorylation of ERK and NF-κB in LPS-Induced J774A.1

Molecules ◽  
2020 ◽  
Vol 25 (18) ◽  
pp. 4058 ◽  
Author(s):  
Hyung Jin Lim ◽  
Seon Gyeong Bak ◽  
Eun Jae Park ◽  
Sae-Kwang Ku ◽  
Soyoung Lee ◽  
...  
Keyword(s):  
Biological Activities ◽  
Edema Formation ◽  
Ear Edema ◽  
Extracellular Signal ◽  
Edema Model ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Factor Α ◽  
Mouse Ear Edema ◽  
Inflammatory Effect

Many studies have reported the biological activities of retrofractamide C (RAC). However, few studies have investigated the anti-inflammatory effect of RAC. In the present study, we investigated the anti-inflammatory effect of RAC using lipopolysaccharide (LPS)-induced J774A.1 cells and a xylene-induced mouse ear edema model. Treatment with RAC decreased LPS-induced nitric oxide (NO) and prostaglandin E2 (PGE2) secretion and inducible NO synthase (iNOS) and cyclooxygenase 2 (COX2) protein expression. It also downregulated the LPS-induced production of interleukin-1β (IL-1β) and interleukin-6 (IL-6) but not tumor necrosis factor α (TNF-α). In the LPS-induced signaling pathway, RAC inhibited the phosphorylation of extracellular signal-regulated kinase (ERK) and nuclear factor kappa light chain enhancer of activated B cells (NF-κB) but not c-Jun N-terminal kinase (JNK) or p38. In a xylene-induced mouse ear edema model, RAC treatment alleviated edema formation and inflammatory cell infiltration. In conclusion, the present study indicates that RAC has the potential to have anti-inflammatory effects and could be a prospective functional food.

scholarly journals Analgesic and Anti-Inflammatory Activities of Resveratrol through Classic Models in Mice and Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Guangxi Wang ◽  
Zhiqiang Hu ◽  
Xu Song ◽  
Qiankun Cui ◽  
Qiuting Fu ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Biological Activities ◽  
Drug Candidate ◽  
Hot Plate ◽  
Sod Activity ◽  
Analgesic Effects ◽  
Mouse Ear ◽  
Anti Inflammatory ◽  
Ear Oedema

Background. Inflammation and pain are closely related to humans’ and animals’ health.Resveratrol(RSV) is a natural compound with various biological activities. The current study is aimed to evaluate the analgesic and anti-inflammatory activities of RSV in vivo. Materials and Methods. The analgesic effects were assessed by the acetic acid-induced writhing and hot plate tests. The anti-inflammatory effects were determined using the xylene-induced mouse ear oedema, the acetic acid-induced rat pleurisy, and carrageenan-induced rat synovitis tests, respectively.Results. The analgesic results showed that RSV could significantly inhibit the number of writhes and improve the time and pain threshold of mice standing on hot plate. The anti-inflammatory results showed that RSV could inhibit the ear oedema of mice. In acetic acid-induced pleurisy test, RSV could significantly inhibit the WBC and pleurisy exudates, could decrease the production of NO, and elevate the activity of SOD in serum. In carrageenan-induced synovitis test, RSV could reduce the content of MDA and elevate the T-SOD activity in serum; RSV could inhibit the expressions of TP, PGE2, NO, and MDA.Conclusion. Shortly, these results indicated that RSV had potent analgesic and anti-inflammatory activities and could be a potential new drug candidate for the treatment of inflammation and pain.

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