The influence of hydrogen sulfide on the structural characteristics of leukocytes mitochondrial apparatus in patients with arterial hypertension

One of the leading causes of arterial hypertension (AH) is mitochondrial dysfunction (MD) – moreover, disorders in regulation of blood pressure occur on the background of progressive energy deficiency. At the same time, the cardioprotective effect of H2S has been proven. In particular, the inhibition of mitochondrial pore opening by hydrogen sulfide plays an important role, and H2S should affect the structural component of MD, namely, the ultrastructure of mitochondria. However, at present, the question of structural changes in the mitochondrial apparatus in patients with hypertension is extremely insufficiently studied. For the study of MD in healthy people and patients with pathology, almost the only objects (in the absence of surgical intervention) are blood cells, in particular leukocytes. Based on the above, the aim of the study was to investigate the effect of a hydrogen sulfide donor on the ultrastructure of the mitochondrial apparatus of leukocytes in patients with arterial hypertension. The effect of a hydrogen sulfide donor on some ultrastructural characteristics of the leukocyte mitochondrial apparatus in patients with hypertension was studied. The examination involved patients (men) with arterial hypertension aged 30-60 years, who were divided into 2 age groups: 30-40 and 40-60 years. Control groups (healthy men without signs of hypertension) were randomized by age. An electron microscopic and morphometric assessment of the structure of mitochondria revealed that under hypertension it undergoes significant changes that depend on the age of the patients. It has been established that the addition of a hydrogen sulfide donor (Full Spectrum Garlic phytopreparation (Swanson Health Products, USA) at a dose of 400 mg per day) to the traditional therapy of hypertension leads to positive changes in the mitochondrial ultrastructure of the studied cells aimed at increasing the energy capacity of the mitochondrial apparatus – the quantity reduction of structurally damaged mitochondria, and with an increase in the duration of treatment – the increase their total number in people of the younger age group (by 57.5%), and in the older age group – by 53.7%. Thus, the indicated effect of H2S significantly depends both on the age of the patients (young people respond more intensively) and on the duration of the hydrogen sulfide donor using (long-term use is accompanied by a more pronounced positive dynamics of changes).

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CHANGES IN THE STRUCTURAL CHARACTERISTICS OF PLATELETS AND LEUKOCYTES IN PATIENTS WITH ARTERIAL HYPERTENSION UNDER THE INFLUENCE OF A HYDROGEN SULFIDE DONOR

EUREKA Health Sciences ◽

10.21303/2504-5679.2020.001288 ◽

2020 ◽

Vol 3 ◽

pp. 33-40

Author(s):

Alexander Kravchuk ◽

Kateryna Rozova

Keyword(s):

Arterial Hypertension ◽

Hydrogen Sulphide ◽

Structural Changes ◽

Structural Characteristics ◽

Age Groups ◽

Age Group ◽

Electron Microscopic ◽

Full Spectrum ◽

Younger Age ◽

Health Products

The aim of the research. The influence of hydrogen sulphide donor on some structural characteristics of platelets and leukocytes in patients with arterial hypertension was studied. Materials and methods. The survey involved patients (men) with hypertension aged 30–60 years, who were divided into 2 age groups: 30–40 years and 40–60 years. Control groups (healthy men without signs of hypertension) were randomized by age. Electron microscopic and morphometric research methods were used in the investigation. Results. Electron microscopic and morphometric evaluation of the structure of these cells has shown that it undergoes significant changes depending on patient’s age. In the persons of the older age group, the cells contained a reduced number of dense δ-granules and increased – α-granules, which may indicate an impaired serotonin balance and increased susceptibility to thrombogenesis. The younger age group presented structural changes in platelets in a reversed order. Adding hydrogen sulphide donor at a dose of 400 mg per day (phytopreparation Full Spectrum Garlic – Swanson Health Products, USA) to the traditional therapy of arterial hypertension leads to positive changes in the ultrastructure of the studied cells aimed at restoring the balance of serotonin, reducing the tendency to thrombogenesis, increasing the energy capacity of the mitochondrial apparatus. Conclusion. The obtained results indicate that detected effects significantly depend on the patient’s age (younger people respond more intensively) and the duration of the use of hydrogen sulphide donor (longer use is accompanied by more pronounced positive changes).

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Choroid plexus carcinoma in childhood: An Light and EM study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010008479x ◽

1991 ◽

Vol 49 ◽

pp. 98-99

Author(s):

Maya Sasi ◽

Joseph M Harb

Keyword(s):

Choroid Plexus ◽

Age Groups ◽

Choroid Plexus Carcinoma ◽

Ependymal Cells ◽

Benign Tumors ◽

Age Group ◽

Electron Microscopic ◽

Pediatric Age Group ◽

Microscopic Features ◽

Choroid Plexus Carcinomas

The normal choroid plexus consists of plexiform pial vessels covered by an epithelial layer which is modified from ependymal cells. Neoplasms of the choroid epithelial cells constitute less than 1% of intracranial neoplasms in all age groups and 2-5% in the pediatric age group. The choroid plexus tumors are divided into two categories, with the benign tumors known as the choroid plexus papillomas and their malignant counterparts being the choroid plexus carcinomas. Only 35 cases of choroid plexus carcinomas have been reported in the literature and only 9 of those included ultrastructural descriptions. In this report, we describe the light and electron microscopic features of three additional cases of this rare neoplasm.

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INFLUENCE OF COMBINED PHARMACOTHERAPY ON CARDIO-VASCULAR SYSTEM OF ARTERIAL HYPERTENSION (IN EXPERIMENT)

Wiadomości Lekarskie ◽

10.36740/wlek202008123 ◽

2020 ◽

Vol 73 (8) ◽

pp. 1712-1716

Author(s):

Svetlana N. Chuhray ◽

Viktoria E. Lavrynenko ◽

Ruzhena M. Matkivska ◽

Tetiana V. Lachtadyr ◽

Valentina M. Hamalii ◽

...

Keyword(s):

Endothelial Cells ◽

Arterial Pressure ◽

Arterial Hypertension ◽

Structural Changes ◽

Vascular System ◽

Ventricular Myocardium ◽

Left Ventricular ◽

Morphometric Study ◽

Left Ventricular Myocardium ◽

Electron Microscopic

DOI: 10.36740/ BOGOMOLETS NATIONAL MEDICAL UNIVERSITY, KYIV, UKRAINE, TARAS SHEVCHENKO NATIONAL UNIVERSITY OF KYIV, KYIV, UKRAINE 3KYIV CITY CLINICAL EMERGENCY HOSPITAL, KYIV, UKRAINE ABSTRACT The aim: Study of the patterns of structural changes in the left ventricular myocardial capillaries of rats with arterial hypertension with combined pharmacotherapy with Bisoprolol and Thiotriazolinum. Materials and methods: Experiments were conducted on 30 line rats with congenital stress-induced arterial hypertension: 10 animals without treatment and 10 animals with treatment. Pharmacological correction of spontaneous arterial hypertension was performed with 20 mg / kg of Bisoprolol and 50 mg / kg of Thiotriazolinum per os once a day. Pharmacotherapy began at 5 months of age, that is, at a time when compensated heart failure was formed in rats with arterial hypertension. Animals were withdrawn from the experiment 100 days after the start of the correction. Control was provided by intact animals (10 rats) of the corresponding age. While extracted from the experiment rats of all experimental groups had their arterial pressure measured using a plethysmograph, electron microscopic examination of the left ventricular myocardium and morphometric study of volumetric and quantitative densities, cross-section area and form factor of micropinocytotic vesicles were conducted. Results: In rats with arterial hypertension after application of Bisoprolol and Thiotriazolinum, arterial pressure significantly decreases in experimental rats compared to animals without correction. The number of capillaries in the myocardium after pharmacotherapy increases up to control values, which shows their reparation. In most endothelial cells, organelles retain their integrity and presence that are characteristic of intact rats. The well-expressed processes of transcytosis are shown by the statistical similarity of the quantitative density and the size of the micropinocytotic vesicles in the endothelial cells of the myocardium capillaries of compared experimental animals. Conclusions: In rats with arterial hypertension, the combination of Bisoprolol and Thiotriazolinum prevents the decrease in the number of capillaries in the myocardium of the left ventricle, promotes the preservation of the ultrastructure of their endothelial cells and maintains the processes of transedothelial transfer of substances at the level of intact animals.

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Relationships between Intelligence and Concept Identification in Adulthood as a Function of Stage of Learning

The International Journal of Aging and Human Development ◽

10.2190/x4x3-46m4-p3pf-ejr2 ◽

1980 ◽

Vol 10 (2) ◽

pp. 187-202

Author(s):

Bert Hayslip

Keyword(s):

Structural Changes ◽

Age Groups ◽

The Elderly ◽

Concept Identification ◽

Age Group ◽

Middle Aged ◽

Differential Ability ◽

And Task

Three groups ( n = 54) of participants aged 17–26, 39–51 and 59–76 were asked to solve two types of concept problems to investigate the intellectual correlates of concept identification as a function of stage of learning in adulthood. Predictions derived from the Gf-Gc theory of Horn and Cattell were partially supported in each age group. Differential ability-performance relations as a function of stage of learning were considerably less potent in the elderly versus the young and middle aged. Comparisons both within and between age groups suggested that a dynamic picture of interactions among organismic and task-related variables is preferable, reflecting structural changes in such relationships with practice and cohort membership.

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AGE AND STRUCTURAL CHANGES IN THE COMPOSITION OF THE PERSONALITY PROPERTIES OF GENDER IN MODERN SOCIETY

“Educational bulletin “Consciousness” ◽

10.26787/nydha-2686-6846-2021-23-2-4-16 ◽

2021 ◽

Vol 23 (2) ◽

pp. 4-16

Author(s):

Galaktionov I.V.

Keyword(s):

Generation X ◽

Generation Y ◽

Structural Changes ◽

Age Groups ◽

Modern Society ◽

General Tendency ◽

Russian Society ◽

Age Group ◽

Gender Patterns ◽

Group Generation

this article examines the issue of gender socialization and identity in modern Russian society, on the basis of analysis of gender characteristics of the personality is compared changes in the structure of the psychological sex (gender) in three age groups (up to 20 years - generation y, 20-40 years - Generation Y and 40-60 years - Generation X), analyzes the causes and possible consequences of changes in gender patterns in different generations. Specifically, the study found that changes in the composition of gender properties and a shift away about traditional stereotypes of feminity and masculinity in the direction of mixing gender roles and qualities occurred in all generations, but the most significant transformations occurred in young men and women of the younger age group (generation) and in middle-aged women (generation Y). The article also presents new data on structural changes in the composition of nuclear personality properties of gender: all males with a general tendency to mix gender qualities in the structure of "real self", the nuclear component of gender consists mainly of their masculine qualities, which is especially evident in men of the middle age group (Generation Y). In women of all generations, the nuclear composition of gender's personality were inconsistent and contradictory, and the choice of masculine qualities in the first places of the gender hierarchy in middle- and older age groups (generation Y and X) may indicate a trend towards further transformation of traditional feminist gender.

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Blood Pressure Levels and Prevalence of Arterial Hypertension in the Population of Residents of the Central Region of Siberia Aged 25–45 Years

Kardiologiia ◽

10.18087/cardio.2019.2.10228 ◽

2019 ◽

Vol 59 (2) ◽

pp. 32-37

Author(s):

N. A. Kovalkova ◽

Yu. I. Ragino ◽

A. D. Hudyakovа ◽

D. V. Denisova ◽

M. I. Voevoda

Keyword(s):

Blood Pressure ◽

Arterial Hypertension ◽

Age Groups ◽

Age Group ◽

Cross Sectional ◽

Men And Women ◽

Mean Values ◽

Younger Age ◽

Grade 3 ◽

Two Age Groups

Purpose:to study blood pressure levels and the prevalence of hypertension in persons aged 25–45 years in Novosibirsk.Materials and methods.A cross-sectional population study in one of typical areas of Novosibirsk was performed in 2013–2016. The study included 479 men and 612 women aged 25–45 years. Arterial hypertension was defined as blood pressure (BP) ≥140 / 90 mmHg according to Russian recommendations (2004). For analysis two age groups were distinguished: 25–34 years and 35–45 years.Results.Mean values of systolic and diastolic BP were significantly lower in women than in men in age groups. In men and women, the analyzed indicators were significantly higher in the older than in the younger age group. Mean values of pulse pressure in men were significantly higher than in women in both age groups, there were no differences in the analyzed index between age groups in either men or women. Optimal BP was more often recorded among women than among men in both age groups. The proportion of persons with normal BP among men was grate than among women in both age groups. In the age group 35–45 years compared with the younger group, in men there was a decrease in incidence of category with BP<140 / 90 mmHg, an increase of the proportion of persons with hypertension grades 1 and 2, the appearance of persons with grade 3 hypertension; in women – an increase of the proportion of individuals with normal, high-normal BP, and with grade 1 hypertension, appearance of individuals with grades 2 and 3 hypertension. Frequency of BP categories ≥140 / 90 mmHg in age group 25–34 years among men was 17.6 %, among women 3.1 % (p<0.0001); in age group 35–45 years among men – 34.7 %, among women – 12.5 % (p<0.0001).Conclusions.Prevalence of hypertension in men was 28 %, in women – 9 %. Favorable tendencies of BP indicators in both sexes were revealed over a 30-year period, while gender differences did not change.

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HCV infection and frequency of distribution.

The Professional Medical Journal ◽

10.29309/tpmj/2019.26.10.4139 ◽

2019 ◽

Vol 26 (10) ◽

pp. 1770-1775

Author(s):

Nighat Aslam ◽

Khalid Nadeem Asif ◽

Sadia Zafar ◽

Amer Jamil

Keyword(s):

Age Groups ◽

Hcv Infection ◽

Molecular Diagnostic ◽

Hcv Rna ◽

Age Group ◽

Duration Of Treatment ◽

Cross Sectional ◽

Hcv Genotypes ◽

Number Of Patients ◽

Chronic Hcv

Worldwide, an estimated 130-170 million people are infected with Hepatitis C. The geographic distribution of HCV infection is highly variable between and within countries. To start the antiviral therapy, it is necessary to find the genotype in order to get better forecast about observed duration of treatment. This also provides the load of virus. Treatments for patients with chronic HCV infection have recently advanced with newly licensed antivirals, which specifically target HCV. Objectives: To find out the prevalence of HCV in Faisalabad region of Pakistan and to evaluate the frequency distribution of various HCV genotypes among those with HCV infection. Study Design: Descriptive cross sectional. Settings: Blood samples were received from Biotech Lab, Pinum cancer hospital Faisal Laboratory, Alshafa lab Jaranwala, Mehran Lab, Samundari, Rashid Lab, Shahkot and Molecular care, Human Molecular Diagnostic Department of Biochemistry University of Agriculture Faisalabad. Period: May 2016 to April 2017. Material and Methods: The data for this study included a total of 382 anti-HCV positive blood sera samples, collected from different collection centers. Nested reverse transcription (RT) PCR was done for the qualitative detection of HCV. RNA using primers that correspond to the relatively conservative 5'UTR noncoding region of the highly mutable HCV were used. Data was analysed using the descriptive statistics. Results: 233 samples were found to be confirm positive for HCV RNA by qualitative PCR. 98 (42%) were females and 135 (58%) were males. The age-group of 36-45 years bear the largest number of HCV patients (37.08%) and smallest number of patients was in the 56-65 years age-group. A total of 87.55% patients belong were below 45 years of age. The genotype 3a, 135 (77%) was the most prevalent form of all HCV genotypes in Faisalabad. A peripheral area of Faisalabad almost same distribution has been observed. The other strains detected were 2a, 29% 3b 11% were males and 5% were female), and none of the patient was detected with 2b, 4a, 5a and 6a genotype of HCV. Conclusion: This data analysis shows that there is no specific relationship of age-groups or genders in case of prevalence of different HCV Genotypes but female patients were found to have higher frequency of HCV infection.

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The Effect of Collagen Mediated Platelet Release on Plasma Prekallikrein Activation

Thrombosis and Haemostasis ◽

10.1055/s-0038-1661014 ◽

1984 ◽

Vol 51 (01) ◽

pp. 037-041 ◽

Cited By ~ 2

Author(s):

K M Weerasinghe ◽

M F Scully ◽

V V Kakkar

Keyword(s):

Platelet Aggregation ◽

Healthy Volunteers ◽

Platelet Rich Plasma ◽

Age Groups ◽

Inhibitory Effect ◽

Age Group ◽

Platelet Factor ◽

Activated Platelets ◽

Platelet Release ◽

Collagen Treatment

SummaryCollagen mediated platelet aggregation caused -5.6 ± 6.7% inhibition and +39.1 ± 15.2% potentiation of prekallikrein activation in plasma from normal healthy volunteers between 20–40 and 50–65 years of age, respectively (n = 15, p <0.01). The amouns of platelet factor-four (PF4) released in the two groups were not significantly different. Collagen treatment in the presence of indomethacin caused +11.5 ± 3.6% and +59.6 ± 19.5% potentiation in the 20–40 and 50–65 age groups respectively (p <0.02). Adrenaline mediated platelet aggregation caused -55.2 ± 7.1% and -35.2 ± 8.3% inhibition in the 20–40 and 50–65 age groups, respectively. Collagen treatment of platelet-deficient-plasma and platelet-rich-plasma in EDTA also caused potentiation of prekallikrein activation.The results indicate that the observed degree of prekallikrein activation after platelet aggregation is a net result of the inhibitory effect of PF4 and the potentiatory effect of activated platelets. The potentiatory effect was greater after collagen treatment as compared to adrenaline treatment, and in the 50–65 age group as compared to the 20–40 age group.

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