scholarly journals Ovitrap Surveillance of Aedes aegypti and Aedes albopictus in Dengue Endemic Areas in Keramat and Shah Alam, Selangor in 2016

2020 ◽  
Vol 17 (3) ◽  
Author(s):  
Noor Afizah A ◽  
Mohd Arif AK ◽  
Nazni WA ◽  
Lee HL
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Species Level ◽  
Dengue Vector ◽  
Entomological Surveillance ◽  
Minimum Number ◽  
Endemic Areas ◽  
The Difference ◽  
Study Sites ◽  
Indoor And Outdoor

Introduction: Entomological surveillance is crucial to determine the abundance of dengue vector and to evaluate breeding areas of Aedes aegypti and Aedes albopictus. The objective of this study is to determine the distribution and breeding preference for both Ae. aegypti and Ae. albopictus in dengue endemic areas. Materials and Methods: Ovitraps surveillance was conducted in two dengue endemic areas; AU2, Keramat and Seksyen 7, Shah Alam, Selangor. A minimum number of 100 ovitraps were deployed for 5 days in the study sites. Samples collected were brought back to the lab and all larvae recovered were identified to species level. Results: The ovitap index (OI) in both localities exceeded the transmission threshold of 10% with the OI recorded ranged from 42.3-79.8% in AU2, Keramat and 16.7-42.9% in Seksyen 7, Shah Alam. Ae. albopictus was the dominant species in AU2 Keramat with the highest ratio Ae. aegypti to Ae. albopictus recorded was 1.00:22.79. Nonetheless, in Seksyen 7, Shah Alam the difference in Ae. aegypti to Ae. albopictus ratio is not really prominent with 1.00:3.61 for ovitraps deployed outdoor and 3.40:1.00 for ovitraps set indoor. It was determined that single infestation of either Ae. aegypti or Ae. albopictus is more frequent for ovitraps deployed indoor and/or outdoor, respectively. It was also determined that mixed infestations were found in this study indicating that both species can oviposit in the same container. Conclusion: This study indicates that OI is still above transmission threshold in both study sites. While Ae. aegypti and Ae. albopictus remain as a dominant indoor and outdoor breeder, respectively, mixed breeding of Aedes species in a same container was also observed.

scholarly journals Insecticide resistance in dengue vectors from hotspots in Selangor, Malaysia

2021 ◽  
Vol 15 (3) ◽  
pp. e0009205
Author(s):  
Rosilawati Rasli ◽  
Yoon Ling Cheong ◽  
M. Khairuddin Che Ibrahim ◽  
Siti Futri Farahininajua Fikri ◽  
Rusydi Najmuddin Norzali ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Insecticide Resistance ◽  
Vector Control ◽  
Aedes Albopictus ◽  
World Health ◽  
Dengue Vector ◽  
Organophosphate Insecticide ◽  
Potential Threat ◽  
Pyrethroid Insecticides ◽  
Dengue Vector Control

Background In Malaysia, dengue remains a top priority disease and usage of insecticides is the main method for dengue vector control. Limited baseline insecticide resistance data in dengue hotspots has prompted us to conduct this study. The present study reports the use of a map on the insecticide susceptibility status of Aedes aegypti and Aedes albopictus to provide a quick visualization and overview of the distribution of insecticide resistance. Method and results The insecticide resistance status of Aedes populations collected from 24 dengue hotspot areas from the period of December 2018 until June 2019 was proactively monitored using the World Health Organization standard protocol for adult and larval susceptibility testing was conducted, together with elucidation of the mechanisms involved in observed resistance. For resistance monitoring, susceptibility to three adulticides (permethrin, deltamethrin, and malathion) was tested, as well as susceptibility to the larvicide, temephos. Data showed significant resistance to both deltamethrin and permethrin (pyrethroid insecticides), and to malathion (organophosphate insecticide) in all sampled Aedes aegypti populations, while variable resistance patterns were found in the sampled Aedes albopictus populations. Temephos resistance was observed when larvae were tested using the diagnostic dosage of 0.012mg/L but not at the operational dosage of 1mg/L for both species. Conclusion The present study highlights evidence of a potential threat to the effectiveness of insecticides currently used in dengue vector control, and the urgent requirement for insecticide resistance management to be integrated into the National Dengue Control Program.

scholarly journals Resistensi Malathion dan Aktivitas Enzim Esterase pada Populasi Nyamuk Aedes aegypti di Kabupaten Pekalongan

2018 ◽  
pp. 61-70
Author(s):  
Dyah Widiastuti ◽  
Bina Ikawati
Keyword(s):  
Enzyme Activity ◽  
Aedes Aegypti ◽  
Hemorrhagic Fever ◽  
Dengue Vector ◽  
Central Java ◽  
Biochemical Assays ◽  
Biochemical Mechanisms ◽  
Study Sites ◽  
F1 Generation ◽  
Dengue Vector Control

Pekalongan regency, Central Java (Indonesia) is one of area which experienced with Dengue Hemorrhagic Fever (DHF) incidence since many years ago. The extensive use of insecticides for dengue vector control has been carried out for long period in Pekalongan Regency. This fact stimulate the need to study the resistence status and  esterase enzyme avtivity of Aedes aegypti to Malathion. The research was conducted in April-November 2014. Insecticide susceptibility assays were performed on F1 generation of wild-caught adult female Ae. aegypti mosquitoes from Simbangkulon Village Buaran Sub district, Kedungwuni Barat Village Kedungwuni Sub dsitrict and Karangsari Village Karanganyar Sub district, with the diagnostic doses of malathion (0,8%). Esterase enzyme activity of these mosquitoes was tested biochemically to find out the mechanisms of resistance to Malathion. Mosquitoes mortality as a result of Malathion effect showed variation in range from 3.33 to 13.75% in all study sites. That result indicated that Ae.aegypti mosquitoes are resistant to Malathion in all study sites . Based on biochemical assays, there was an increase if esterase enztyme activity of Ae aegypti in all study sites.. Therefore, alpha esterase enzyme activity could be associated with the mechanisms which responsible to malathion resistance in Ae. aegypti in Pekalongan. The results of this study provided the information about the malathion resistance status of Ae. aegypti in Pekalongan, and biochemical mechanisms which essential for monitoring the insecticide resistance in this area.  

scholarly journals First Evidence of the Implication of Cuticular Based Mechanisms in Aedes Aegypti Populations Resistant To Pyrethroids and DDT in Cameroon

2021 ◽  
Author(s):  
Borel DJIAPPI TCHAMEN ◽  
Nana-Ndjangwo Stella Mariette ◽  
Vasileia Balabanidou ◽  
Konstantinos Mavridis ◽  
Makoudjou Idene ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Pyrethroid Resistance ◽  
Cuticular Hydrocarbon ◽  
Target Site ◽  
Detoxification Enzymes ◽  
The Difference ◽  
Susceptible Populations ◽  
Cuticular Resistance

Abstract Pyrethroid resistance is now expanding in the two main arboviruses vectors Aedes aegypti and Aedes albopictus from Cameroon. Although recent studies suggested the implication of target site (kdr) resistance and overexpression of detoxification enzymes as key mechanisms, the implication of additional mechanisms such as cuticular resistance has not been investigated. The present study assesses the possible implication of cuticle in Aedes species resistance to pyrethroids. High cuticular hydrocarbon (CHC) content was detected in Ae. aegypti populations from Douala and Yaoundé. The difference (38% increase) between the Douala and susceptible populations was found to be statistically significant.The study confirms the implication of cuticular-based mechanisms in resistant to pyrethroids and DDT Aedes aegypti mosquitoes from Cameroon.

Efektivitas Ekstrak Umbi Gadung (Dioscorea Hispida Dents) Dalam Pengendalian Larva Aedes Aegypti Dan Aedes Albopictus

2018 ◽  
Vol 3 (3) ◽  
pp. 515
Author(s):  
Ratna Sari Dewi
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
Experimental Research ◽  
Hemorrhagic Fever ◽  
Control Group ◽  
Anova Analysis ◽  
Natural Insecticides ◽  
The Difference ◽  
Short Time ◽  
Tuber Extract

<p><em>Dengue Hemorrhagic Fever is one of the diseases that disease quickly and can cause death in a short time. One of the natural insecticides in killing Aedes aegypti larvae and Aedes alboipictus is by using tuber bulbs extract</em><em>.</em><em>The purpose of this research is to know the difference of various concentrations of tuber bulbs extract on the death of Aedes aegypti larva and Aedes alboipictus. This design of research is experimental research </em><em>and Anova analysis</em><em>. The number of population in this study as many as 780 larvae. The concentration used in this research is 0.02%, 0.05%, 0.10%, 0.15%, 0.20%. The results showed that the average deaths of the two different test larvae for the lowest concentrations of 0.02% the average death of the test larvae were 5.80 deaths in the Aedes aegypti larvae and 4.80 deaths in the Aedes albopictus larvae.</em><em> </em><em>The conclusion of this research is the difference of concentration 0,02%, 0,05%, 0,10%, 15%, and 0,20% Gadung Umbi extract to Aedes aegypti and Aedes albopictus larvae mortality and concentration correlation 0,02 %, 0.05%, 0.10%, 0.15%, and 0.20% of Gadung Tuber extract on the death of Aedes aegypti and Aedes albopictus larvae.</em><em></em></p><p> </p><p>Demam Berdarah  <em>Dengue </em> merupakan salah satu penyakit yang perjalanan penyakitnya cepat dan dapat menyebabkan kematian dalam waktu singkat. Salah satu insektisida alami dalam membunuh larva <em>Aedes</em><em> </em><em>aegypti</em><em> </em>dan <em>Aedes</em><em> </em><em>alboipictus</em> adalah dengan menggunakan ekstrak umbi gadung. Tujuan penelitian ini untuk mengetahui perbedaan berbagai konsentrasi ekstrak umbi gadung terhadap kematian larva <em>Aedes</em><em> </em><em>aegypti</em><em> </em>dan <em>Aedes</em><em> </em><em>alboipictus</em><em>.</em> Jenis penelitian ini merupakan penelitian ekperimen dengan menggunakan rancangan penelitian <em>Posttest Only Control Group.</em><em> </em>Jumlah populasi dalam penelitian ini sebanyak 780 ekor larva. Konsentrasi yang digunakan pada penelitian ini yaitu 0,02%, 0,05%, 0,10%, 0,15%, 0,20%. Penelitian ini menggungakan analisis Anova satu arah. Hasil penelitian menunjukkan bahwa rata-rata kematian kedua larva uji yang berbeda untuk konsentrasi terendah 0,02% rata-rata kematian  larva uji sebesar 5,80 kematian pada larva nyamuk <em>Aedes aegypti</em> dan 4,80 kematian pada larva nyamuk <em>Aedes albopictus</em>. Kesimpulan dari hasil penelitian ini adalah adanya perbedaan konsentrasi 0,02%, 0,05%, 0,10%, 15%, dan 0,20% ekstrak Umbi Gadung<em> </em>terhadap kematian larva <em>Aedes aegypti </em>dan <em>Aedes albopictus</em><em> </em>serta adanya hubungan konsentrasi 0,02%, 0,05%, 0,10%, 0,15%, dan 0,20% ekstrak Umbi Gadung terhadap kematian larva <em>Aedes aegypti </em>dan <em>Aedes albopictus</em>.</p>

scholarly journals Dengue virus in Aedes aegypti and Aedes albopictus in urban areas in the state of Rio Grande do Norte, Brazil: Importance of virological and entomological surveillance

PLoS ONE ◽  
2018 ◽  
Vol 13 (3) ◽  
pp. e0194108 ◽  
Author(s):  
Arlinete S. Medeiros ◽  
Diego M. P. Costa ◽  
Mário S. D. Branco ◽  
Daíse M. C. Sousa ◽  
Joelma D. Monteiro ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Dengue Virus ◽  
Aedes Albopictus ◽  
Urban Areas ◽  
Rio Grande ◽  
The State ◽  
Entomological Surveillance

scholarly journals Avaliação da produtividade dos criadouros do Aedes aegypti (L.) e Aedes albopictus (Skuse) através dos dados da vigilância em Parati – RJ.

2012 ◽  
Vol 5 (3) ◽  
pp. 223-226
Author(s):  
Eduardo Dias Wermelinger ◽  
Adilson Benedito Almeida ◽  
Ciro Villanova Benigno ◽  
Aldo Pacheco Ferreira
Keyword(s):  
Aedes Aegypti ◽  
Vector Control ◽  
Urban Environment ◽  
Aedes Albopictus ◽  
Surveillance Data ◽  
Dengue Vector ◽  
Tree Holes ◽  
Dengue Control ◽  
Dengue Vector Control

Esse estudo avaliou a produtividade dos criadouros de Aedes aegypti (Linnaeus) e Aedes albopictus (Skuse) no município de Parati através dos dados da vigilância entomológica obtidos pelos métodos preconizados pelo Programa Nacional de Controle da Dengue (PNCD). O maior número de pupas de A. aegypti foi coletado nos ralos (31%), garrafas e latas (23%); e com A. albopictus o maior número foi nas garrafas, latas (24%) e vasos de planta (21%). Do total de pupas obtidas, 84,3% e 79,3% das pupas de A. aegypti e A. albopictus respectivamente foram coletadas em pequenos reservatórios: garrafas, vasos de plantas, pneus, bromélias, oco de árvores e ralos. Essas produtividades contradizem a literatura e podem ser explicadas pelas limitações de acesso aos grandes reservatórios e falha na capacitação dos agentes. Esse estudo chama atenção para a importância que os pequenos reservatórios podem ter na densidade do vetor no meio urbano nacional e conclui que a metodologia de vigilância entomológica usada tem sido ineficaz para identificar os grandes e mais produtivos criadouros. Essa conclusão sugere que essa ineficácia pode ser um fator importante pelos insucessos no combate a dengue no Brasil. Evaluation of Breeding Productivity from Surveillance Data for Aedes aegypti (Linnaeus) and Aedes albopictus (Skuse) in Parati, RJ, Brazil Abstract. This study evaluated the pupa-productivity of the entomologic surveillance according methodology used by the official Program for Dengue Control in Brazil (PDCB) for Aedes aegypti (Linnaeus) and Aedes albopictus (Skuse) in Parati, RJ, Brazil. The highest number of A. aegypti pupae were found in drains (31%), bottles and cans (23%); and the highest number of A. albopictus pupae were found in bottles and cans (24%), and plant vases (21%). Of the total pupae identified 84.3% and 79.3% of A. aegypti and A. albopictus respectively were collected in small receptacles: bottles, plants vase, tires, bromeliads and tree holes. These productivities are not supported by literature and can be explained by the restrictions of access for the bigger receptacles and lack of training of the agents. The study points out the importance which the small receptacles can have on vector densities in urban environment despite of their productivity and conclude that the entomology surveillance methodology for dengue used in PDCB has no efficacy to identify the bigger and more productive receptacles. This conclusion suggests that this inefficacy can be an important factor for the failures on dengue vector control in Brazil.

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