scholarly journals Assessment on Functionality and Viability of β cells Following Repetitive Dosage Administration of Ethanolic Extracts of Andrographis paniculata on Streptozotocin-induced Diabetic Rats

2020 ◽  
Vol 9 (1) ◽  
Author(s):  
Abdul Razak K ◽  
Mariam A ◽  
Amirin S ◽  
Mohd Zaini A
Keyword(s):  
Blood Glucose ◽  
Plant Extracts ◽  
Fasting Blood Glucose ◽  
Insulin Level ◽  
Diabetic Rats ◽  
Andrographis Paniculata ◽  
Β Cells ◽  
Glucose Levels ◽  
Ethanolic Extracts ◽  
Before And After

Introduction: The study was done at the aim to assess the functionality and viability of the β cells of the streptozotocin-induced diabetic rats model following repetitive dosage of administration of ethanolic extracts of Andrographis paniculata. Materials and Methods: The diabetic rats were treated with the extracts for fourteen days and at the dose given was 500 mg/kg twice daily. The assessments were made on fasting blood glucose, insulin, and immunohistochemical aspect of β cells before and after treatment. Results: The results showed that there was a significant reduction on fasting blood glucose levels in metformin, 95% and 50% ethanolic plant extracts-treated groups but on insulin level only 95% and 50% ethanolic extracts-treated groups gave a significant reduction (p<0.05). Immunohistochemical assessments revealed that all extract groups and metformin-treated were significantly increased in the population of β cells (p<0.01). Conclusion: This study revealed that the plant extracts showed an ability to promote the growth or rejuvenate the STZdestructed β cells and in turn lower the blood glucose level.

High Throughput Study for Molecular Mechanism of Metformin PreDiabetic Protection via Microarray Approach

2021 ◽  
Vol 21 ◽  
Author(s):  
Asma Y. Alrawashdeh ◽  
Mohammad A. AL Shhab ◽  
Malek A. Zihlif
Keyword(s):  
Blood Glucose ◽  
Test Group ◽  
Diabetic Rats ◽  
P Value ◽  
Protective Effects ◽  
Β Cells ◽  
Insulin Levels ◽  
Glucose Levels ◽  
Before And After ◽  
Male Wistar Rats

Background:: Metformin is a biguanide that exhibits an antidiabetic, anticarcinogenic and anti-inflammatory properties. Despite well-known pancreatic protective effects, metformin's influence on pancreatic islet β-cell is yet considerably unknown. Protecting the functional insulin-producing β-cells in the pancreas is a key therapeutic challenge in patients with type 1 (T1DM) or type 2 diabetes mellitus (T2DM). Objective:: Current study aimed to analyze the protective effects of metformin on streptozocin-induced diabetic rats in T1DM in hepatic tissues. Methods: : In the present study, male wistar rats (n=24) were randomly assigned into 2 groups (n=12 for each control and test) by which metformin (100 mg/kg/day) were given for 7 weeks. Afterwards, diabetes was induced by streptozocin (STZ) at a single dose of (150 mg/kg). Blood glucose was daily examined before and after STZ induction. The animals were euthanized by cervical dislocation 5 days after streptozocin injection, after which liver and pancreas were harvested from each rat. Results: : The biochemical analyses revealed that metformin resulted in significantly reduced plasma glucose levels and higher pancreatic insulin levels in the test group. Using a restrictive cut-off of at least 2-FC and an adjusted p-value (qvalue) of ≤0.05 a sum of 747 genes for the metformin group were shown to be differentially regulated compared to controls (320 Down and 427 Up), by which they were obtained from the liver. Furthermore, evidence is attained that metformin may hinder the loss of critical β-cells by reducing inflammatory and apoptosis signaling, promoting fatty acid β-oxidation and inducing metabolism. Conclusion: : Collectively, this study has demonstrated a decrease in blood glucose levels and a rise in insulin-levels and thus a consequent prophylactic effects in metformin-given STZ-induced diabetic rats.

scholarly journals Antidiabetic and Modulatory Effect of Ethanol Extract of Neem Leaf on Some Essential Biochemical Parameters of Streptozotocin-induced Diabetic Rats

2020 ◽  
pp. 1-11
Author(s):  
Ezeigwe Obiajulu Christian ◽  
Ezeonu Francis Chukwuemeka
Keyword(s):  
Blood Glucose ◽  
Acute Toxicity ◽  
Biochemical Parameters ◽  
Biochemical Analysis ◽  
Fasting Blood Glucose ◽  
Lethal Dose ◽  
Ethanol Extract ◽  
Insulin Level ◽  
Diabetic Rats ◽  
Glucose Levels

The study was conducted to investigate the modulatory activity of ethanol extract of neem leaf on the essential biochemical parameters of streptozotocin-induced diabetic rats. The 24-hour acute toxicity test of the orally administered ethanol extract was determined using Finney’s method. Diabetes was induced in the rats by a single intraperitoneal dose of 50 mg/kgbw of streptozotocin (STZ). Blood was collected after 28 days of treatment and used for biochemical analysis. Biochemical analysis was done using standard biochemical methods. The acute toxicity study of the ethanol extract reveal that the median lethal dose was 5.0 g/kg body weight. The anti-diabetic study carried out for a period of 28 days showed a significant (p<0.05) reduction in the fasting blood glucose levels of the animals treated with ethanol extract compared with the diabetic untreated rats. The urea, creatinine, ALT, AST, ALP and bilirubin levels increased significantly (p<0.05) in the diabetic untreated rats compared with the groups treated with the ethanol extract of neem leaf. The serum alpha-amylase activity of the treatment groups decreased significantly (p<0.05) compared with the diabetic untreated group. The diabetic untreated rats showed a significant (p<0.05) decrease in their insulin level compared with the insulin levels of animals treated with the ethanol extract of neem leaf. The ethanol extract of neem leaf is efficacious in lowering blood glucose level in STZ-induced diabetic rats by regenerating the beta cells integrity which was seen from the increase in the insulin level of the extract treated rats. It modulates the essential biochemical parameters of diabetic rats favourably towards recovery and improved health.

scholarly journals Effect of Cyperus squarrosus plant extracts on diabetic neuropathy in Streptozotocin (STZ)–induced diabetic rats

2021 ◽  
Vol 12 (3) ◽  
pp. 1915-1921
Author(s):  
Raghavendra H G ◽  
Ravipraksh P ◽  
Devanna N
Keyword(s):  
Blood Glucose ◽  
Diabetic Neuropathy ◽  
Plant Extracts ◽  
Fasting Blood Glucose ◽  
Elevated Serum ◽  
Serum Glucose ◽  
Diabetic Rats ◽  
Control Group ◽  
Glucose Levels ◽  
Blood Glucose Levels

The present study was designed to evaluate the effects of Cyperus squarrosus plant extracts in streptozotocin-induced diabetic neuropathy. Diabetes was induced by a single administration of streptozotocin (65 mg/kg/intraperitoneally). After 72 hours to measure the fasting blood glucose levels, blood glucose levels more than 220 mg/dl, consider diabetic rats. Animals were divided into six groups, animals untreated with STZ were kept in a normal control group. STZ-induced diabetic rats orally administered with ethanolic and aqueous plant extracts (EECS & AECS) dose 200 & 400 mg/kg, p.o. for eight weeks, Rats were subjected to evaluate serum glucose, biochemical parameters, and test were performed on the initial day, 2n, 4th 6th and 8th week in all groups to evaluate the neuropathy by Eddy’s hot plate and tail immersion test respectively. Diabetic animals were treated with Glibenclamide (5 mg/kg) and plant extracts (200 & 400 mg/kg )for 8 weeks. Groups treated with Glibenclamide (5 mg/kg) and Cyperus squarrosus extracts (200 and 400 mg/kg) significantly reduced elevated serum glucose level & lipid profiles and restored the reduced body weight. After completion of work, we observe the changes in fasting serum glucose, triglysaroids LDL, cholesterol levels are significantly reduced in animals treated with aqueous extract 400mg/kg. Other side animals treated with extracts decrease in the severity of diabetic neuropathy compare with untreated diabetic rats. Finally concluded that Cyperus squarrosus plant exhibit anti-diabetic property and beneficial protection against neuropathy in STZ induced diabetic rats.

Anti-diabetic activity of diphenhydramine in diabetic rats

2020 ◽  
Vol 11 (4) ◽  
pp. 5067-5070
Author(s):  
Pang Jyh Chayng ◽  
Nurul Ain ◽  
Kaswandi Md Ambia ◽  
Rahim Md Noah
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Fasting Blood Glucose ◽  
Insulin Level ◽  
Diabetic Rats ◽  
Group Iv ◽  
Diabetic Rat ◽  
Control Group ◽  
Group I

The purpose of this project is to study the anti-diabetic effect of on a diabetic rat model. A total of Twenty male Sprague rats were used and it randomly distributed into four groups which are Group I: , Group II: negative control, Group III: and Group IV: and . In diabetic model were induced with via injection at the dosage of 65mg/kg. and FBG (Fasting Blood Glucose) level of diabetic rats were assessed every three days. Blood was collected via cardiac puncture at day 21 after the induction of treatment. Insulin level of the rats was assessed with the Mercodia Rat Insulin ELISA kit. FBG level of group I (12.16 ±3.96, p&lt;0.05) and group IV (11.34 ±3.67, p&lt;0.05) were significantly decreased. Meanwhile, the for all rats did not show any significant increase. However, the insulin level was escalated in group IV (0.74+0.25, p&lt;0.05) significantly. The present study shows that the and the combination of and lowered blood glucose level and enhanced insulin secretion.

scholarly journals Evaluation of the Effect of Macronutrients Combination on Blood Sugar Levels in Healthy Individuals

2021 ◽  
Author(s):  
Berrak BASTURK ◽  
Zeynep KOC OZERSON ◽  
Aysun YUKSEL
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Protein Source ◽  
Carbohydrate Source ◽  
Glucose Levels ◽  
Positive Effects ◽  
Blood Glucose Levels ◽  
Before And After ◽  
Sugar Levels ◽  
Mean Blood Glucose

Background: The positive effects of blood glucose levels should be demonstrated in healthy or type 2 diabetic individuals who can be recommended to consume macronutrients (protein + fat) with carbohydrates. Therefore, at the end of the research planned with the amount of carbohydrates and fats that can be consumed in a meal, we aimed to recommend the consumption of food with high protein content egg together with the carbohydrate source. Methods: The study was carried out from Nov 2017 to Apr 2018 by looking at fasting blood glucose levels using feeding 2 different test foods on a minimum of 8 h of fasting in the Haliç University Sutluce Campus, Istanbul, Turkey. Before and after the carbohydrate and carbohydrate + protein source, blood glucose was measured from the fingertip for 3 days in 30 min, 60 min and 120 min periods. The average of 3 days was used in the analysis. Results: Blood glucose values were compared after individuals were given carbohydrate and carbohydrate + protein source. The mean blood glucose value 60 min after the carbohydrate administration was significantly higher than the average blood glucose value 60 min after the carbohydrate + protein administration (P= 0.006). Conclusion: A protein-containing diet positively affects the glycemia response and can recommend it. In individuals with diabetes, they should focus on the effects of proteins to achieve glycemia control.

scholarly journals Effect of Sago Analog Rice and Red Bean Diet to β Cells Pancreas Refinement in STZ-NA Induced Diabetic Rats

2020 ◽  
pp. 667-673
Author(s):  
Sri Budi Wahjuningsih ◽  
Haslina Haslina ◽  
Agus Tri Putranto ◽  
Mita Nurul Azkia
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Insulin Level ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Pancreatic Β Cell ◽  
Β Cells ◽  
Β Cell ◽  
Red Bean

The study aims to determine the effect of sago analogue rice and red beans in diabetic rats to repair pancreatic β-cells. Thirty-five males Wistar rats were divided into 5 groups: normal group diet (STD), the diabetic group (STDD) with a standard feed diet, the diabetic group with mentik wangi rice (MWRD), the diabetic group with sago analogue rice (SARD) and the diabetic group with sago analogue rice with the addition of 10% red bean flour (SARKBD). All groups were analysed for dietary interventions, blood glucose level, insulin level for HOMA-β and HOMA S indices and measurement of insulin level by using IHC analysis. In addition, short-chain fatty acids (SCFA) analysis was performed in the caecum. This study showed that decreasing blood glucose level shown in SARD and RASKBD groups. The pancreatic β-cell number indicated an increase in the SARD group compares to the STDD group. The pool total of SCFA in SARD group was the highest among of all groups, as well as the acetate, propionate and butyrate pools. These results indicate that the sago analogue rice diet could repair and increase the expression of pancreatic β-cell through absorption inhibition mechanisms and by increasing insulin sensitivity and the SCFA level.

scholarly journals The Effect of Belimbing Wuluh (Averrhoa Bilimbi L.) and Red Guava (Psidium Guajava L.) Extract on Fasting Blood Glucose Levels of Diabetic Wistar Rats Model

2019 ◽  
Vol 5 (2) ◽  
pp. 57
Author(s):  
Yesi Warisman ◽  
Arinda Lironika Suryana ◽  
Zora Olivia
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Glucose Level ◽  
Wistar Rats ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Control Group ◽  
Standard Diet ◽  
Glucose Levels ◽  
Blood Glucose Levels

Diabetes mellitus is a metabolic disease with special characteristic which increased blood glucose level (hiperglicemic), its happens because of the abnormality of insulin secretion, insulin mechanisme or both of them. Belimbing wuluh and red guava extract can become alternative functional drink that contain rich of vitamin C to help controlling blood glucose level. The aimed of this study was to determined the effect of belimbing wuluh and red guava extract on blood glucose levels of Rattus novergicus strain Wistar diabetic model. This research was an true experimental study with a randomized post-test control group design. Twentyfour (24) male wistar strain rats weighing 200-250 grams, aged 2-3 months, were divided into 4 groups. The control group (K-) non-diabetic rats, the control group (K+) diabetic rats given standard diet, and the treatment group diabetic rats given standard diet and belimbing wuluh and red guava extract intervetion doses 10,7 ml/day (P1) and 21ml/day (P2). Measurement of pretest posttest fasting blood glucose levels was carried out using biolyzer. The data were analyzed by One Way Anova test. The results showed that there were significant differences in delta pretest and posttest fasting blood glucose levels between all groups. Blood glucose levels decreased at 55.6%. In conclusion, belimbing wuluh and red guava extract 21ml/day most effectively decreased fasting blood glucose levels in diabetic wistar rats moldel.   Keywords: belimbing wuluh, blood glucose level, diabetes mellitus, red guava

scholarly journals Polygonatum sibiricum extract exerts inhibitory effect on diabetes in a rat model

2021 ◽  
Vol 18 (7) ◽  
pp. 1493-1497
Author(s):  
Lai-zeng Yu ◽  
Xue-peng Zhang ◽  
Ying-xin Wang
Keyword(s):  
Lipid Peroxidation ◽  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Insulin Level ◽  
Serum Levels ◽  
Inhibitory Effect ◽  
Diabetic Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Dependent Manner

Purpose: To investigate the effect of Polygonatum sibiricum extract (PSE) on streptozotocin-induced diabetic rats. Methods: PSE was obtained by steeping the dried Polygonatum sibiricum in water at 60 oC three times, each for 1 h, before first drying in an oven at 100C and then freeze-drying the final extract, thus obtained. Diabetic model rats were prepared by a single intraperitoneal injection of a freshly prepared solution of streptozotocin (50 mg/kg). The rats were randomly divided into 6 groups of ten rats each: negative control, normal control, reference (glibenclamide1 mg/kg) as well as PSE groups, (35, 70 and 140 mg/kg). Blood glucose and plasma insulin levels were measured to determine antihyperglycemic effect. Oxidative stress was evaluated in liver and kidney by their antioxidant markers, viz, lipid peroxidation (LPO), superoxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (GPx) and catalase (CAT). Blood serum levels of creatinine and urea were determined in both diabetic control and treated rats. Results: Compared with diabetic rats, oral administration of PSE at a concentration of 120 mg/kg daily for 30 days showed a significant decrease in fasting blood glucose (118.34 ± 3.29 mg/dL) (p < 0.05) and increased insulin level (12.86± 0.62 uU/mL, p < 0.05). Furthermore, it significantly reduced biochemical parameters (serum creatinine, 0.83 ±0.21 mg/dL, p < 0.05) and serum urea (43.26±1.42 mg/dL, p < 0.05). Conclusion: The results suggest that PSE may effectively normalize impaired antioxidant status in streptozotocin-induced diabetes in a dose-dependent manner. Thus, PSE has a protective effect against lipid peroxidation by scavenging free radicals, restoration of insulin function, and reduction of the incidence of complications.

Dioscorea dumetorum-Fed Rats Exhibited Decreased Body Weight, Blood Glucose, and Insulin in STZ-induced Diabetes

2014 ◽  
Vol 4 (2) ◽  
pp. 87 ◽  
Author(s):  
Henrietta Aritetsoma Ogbunugafor ◽  
Emmanuel Emeka Ilodigwe ◽  
Daniel Lotanna Ajaghaku ◽  
Chinwe Nonyelum Ezekwesili ◽  
Chike Samuel Okafor ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Feed Intake ◽  
Weight Reduction ◽  
Blood Glucose Concentration ◽  
Insulin Level ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Male Rats ◽  
Glucose Levels ◽  
Blood Glucose Levels

Background: Preventive measures that could slow down the rising incidences of diabetes mellitus are essential. The use of neglected local foods, which have effects on this chronic disease beyond basic nutrition as dietary controls, is desirable.Objective: The effect of Dioscorea dumetorum (Kunth) Pax (Dioscoreaceae) feed on satiety, weight, blood glucose, and insulin levels were investigated in streptozotocin-induced diabetic rats.Methods: Twenty adult male rats in four groups of five were used for the experiment. Three groups – D. dumetorum, glibenclamide, and standard pellet-fed rats were induced with diabetes by i.p. administration of 50mg kg-1 streptozotocin, while the fouth group (?) served as a non-diabetic control. D. dumetorum was fed at 15g daily for ten days before induction, and after induction, feeding continued. Glibenclamide was orally administered 5mg kg-1 daily. Both the untreated and non-diabetic rats were kept on standard rat pellets. Feed intake, weight, and blood glucose concentration were monitored daily, while insulin level was measured on day two and day six after inductions. Results: Average feed intake for non-diabetic rats was 15g for D. dumetorum per day, which dropped to 10.3g after induction of diabetes. Weight of normal non-diabetic rats consistently increased (142.61 ± 4.37g – 169.43 ± 8.61g) for the duration (17 days) of the experiment. The D. dumetorum-fed rats showed weight reduction of 5.4%, glibenclamide 4.0%, and untreated diabetic 6.15%. Non-diabetic rats blood glucose levels ranged between 70 to 100mg dL-1. Streptozotocin (STZ) (i.p.) administration increased blood glucose levels from 370% to 626% in the rats. D. dumetorum-fed rats showed reduced (p<0.05) blood glucose levels of 22.6%. Glibenclamide had 5.5% reduction (p<0.05). Insulin was absent in D. dumetorum-fed rats, whereas 0.95ng ml-1 of insulin was detected in glibenclamide-administered rats. These quantities were lower (p<0.001) than 1.40ng ml-1 in the non-diabetic rats. Conclusion: This study revealed that D. dumetorum tuber caused decreased hunger, weight reduction, and displayed hypoglycemic property in diabetic rats, even after heat treatment. Its probable mechanism of anti-hyperglycemic activity might not be through increased insulin secretion.Key words: D. dumetorum, streptozotocin-induced diabetes, weight, blood glucose, insulin.

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