scholarly journals ANTIOXIDANT SYSTEM AND CELLULAR-HUMORAL PARAMETERS IN DRY COWS

2021 ◽  
Vol 247 (3) ◽  
pp. 146-149
Author(s):  
I.R. Minnibayev ◽  
◽  
R.U. Zaripov ◽  
A.A. Klenova ◽  
A.M. Alimov ◽  
...  
Keyword(s):  
Free Radical ◽  
Antioxidant System ◽  
Catalase Activity ◽  
Respiratory Function ◽  
Free Radical Oxidation ◽  
The State ◽  
Total Lipids ◽  
Radical Oxidation

Studies of the state of the antioxidant system and cellular-humoral parameters in dry cows were carried out. They showed a tendency to develop anemia, a decrease in the respiratory function of the blood and an increase in free radical oxidation, which was expressed in a decrease in the level of ceruloplasmin, an increase in catalase activity, the content of total lipids and malondialdehyde.

scholarly journals PSX-3 Antioxidant system characteristics in Saanen goats depending on performance

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 461-462
Author(s):  
Polina D Bokhan ◽  
Alesya A Bakhta ◽  
Larisa Y Karpenko ◽  
Anna I Kozitcyna ◽  
Anna B Balykina ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Free Radical ◽  
Milk Yield ◽  
Antioxidant System ◽  
Free Radical Oxidation ◽  
Dairy Goat ◽  
Blood Levels ◽  
Lactation Performance ◽  
Radical Oxidation ◽  
System Characteristics

Abstract Dairy goat breeding is widespread worldwide. Goat milk and other derivative products are an important part of nutrition. Dairy products are the most important part of goat breeding; therefore, lactation performance is a relevant issue. The purpose of this study was to evaluate antioxidant system characteristics in Saanen goats depending on lactation performance. The experiment was conducted in the north-western region of Russian Federation, in the laboratories of the biochemistry and physiology departments at FSBEI of Higher Education «SPbSAVM». The three experimental groups included 30 Saanen goats each, 2nd–3rd lactations, selected using matched pairs method. 1st group included low milk producing ability goats (< 600 kg of milk yield per year), 2nd group included average milk producing ability goats (600–800 kg of milk yield per year), 3rd group included high milk producing ability goats (>800 kg of milk yield per year). The blood samples were taken once: at peak lactation performance (45 days after parturition). The blood levels of lipid peroxygenation markers (malondialdehyde, dienketone and conjugated dienes) and superoxide dismutase and catalase activity were assessed by standard methods. Results presented as mean±standard error of the mean. Student’s t-test was used after proving normal distribution. Level of significance is labeled as P < 0.05. The results are displayed in table 1 and 2. After analyzing the received data of antioxidant system characteristics, we found a high intensity of free-radical oxidation in high milk producing ability goats. This results in oxidative stress development. Activity of anti-oxidizing enzymes was elevated; therefore, free-radical oxidation is intensive. Consequently, these data allow us to take into consideration exogenous anti-oxidizing agents administration in high milk producing ability goats in order to reduce oxidative stress.

The state of free radical oxidation in the tissue of primary vulvar cancer and of its recurrence.

2014 ◽  
Vol 32 (15_suppl) ◽  
pp. e22234-e22234
Author(s):  
Ekaterina Igorevna Surikova ◽  
Irina Aleksandrovna Goroshinskaya ◽  
Galina Andreevna Nerodo ◽  
Ekaterina Alekseevna Nerodo ◽  
Elena Vladimirovna Shalashnaya ◽  
...  
Keyword(s):  
Free Radical ◽  
Vulvar Cancer ◽  
Free Radical Oxidation ◽  
The State ◽  
Radical Oxidation

scholarly journals Genetic peculiarities of free radical oxidation of lipids and proteins in the semen of breeding boars

Biologija ◽  
2018 ◽  
Vol 64 (3) ◽  
Author(s):  
Svitlana Polishchuk ◽  
Svitlana Tsekhmistrenko ◽  
Vitalii Polishchuk ◽  
Oksana Tsekhmistrenko ◽  
Nataliia Ponomarenko ◽  
...  
Keyword(s):  
Free Radical ◽  
Antioxidant System ◽  
Free Radical Oxidation ◽  
Reproductive Function ◽  
Oxidative Modification ◽  
Peroxide Oxidation ◽  
Large White ◽  
Radical Oxidation ◽  
Key Enzyme ◽  
Synthetic Line

Complex research into biochemical aspects of the functioning of the antioxidant system for the protection of sperm of the breeding boars has established that the intensity of the flow of peroxide oxidation of lipids in germ cells is much higher than in the extracellular space. Reactions of free radical peroxide oxidation of proteins and lipids in the semen of healthy breeding boars are characterized by a stable level of activity that is necessary for the normal course of implementation of processes of the reproductive function. All components of the antioxidant system stay in mutual compensatory ratios under physiological conditions. As a rule, reducing the concentration or activity of some antioxidant enzymes leads to corresponding changes in others. The main antioxidants of the germ cell genomes are superoxide dismutase and ceruloplasmin. Catalase is a key enzyme that neutralizes H2O2 in the semen fluid of breeding boars. The content of total proteins in the semen of the synthetic line SS23 animals is greater (p < 0.05) than in the breeding boars of the large white breed. The processes of the oxidative modification of proteins in the animals body of the synthetic line runs more intensely, as it is evidenced by the higher content of the aldehyde and ketodinitrophenyl hydrazones of the main and neutral character in the sperm cytoplasm.

scholarly journals EFFICIENCY OF THE COMPLEX OF THERAPEUTIC ACTIVITIES OF CHRONIC GYNGIVITIS IN PATIENTS WITH CHRONIC GASTRODUODENITE

2018 ◽  
Vol 14 (2) ◽  
pp. 25-29
Author(s):  
Миляуша Кабирова ◽  
Milyausha Kabirova ◽  
Лариса Герасимова ◽  
Larisa Gerasimova ◽  
Сафия Каримова ◽  
...  
Keyword(s):  
Free Radical ◽  
Light Emission ◽  
Free Radical Oxidation ◽  
Positive Control ◽  
Negative Control ◽  
The State ◽  
Radical Oxidation ◽  
Oral Liquid ◽  
Primary 52 ◽  
Therapeutic Activities

Subject. The article presents clinical, biochemical, immunological and microbiological results of a complex of therapeutic measures of chronic gingivitis in patients with chronic gastroduodenitis. The aim of the study was to study the effectiveness of treatment of chronic gingivitis in patients with chronic gastroduodenitis with the use of the proposed method. Methodology. The subject of the study was 155 persons aged 19 to 25 years with diagnosed chronic gingivitis and chronic gastroduodenitis, which were divided by stratified randomization into three groups: 1 group (primary) - 52 patients; 2 group (positive control) - 52 patients; Group 3 (negative control) - 51 patients. All groups were comparable in age (F = 2,355, p = 0,092) and sex (χ2 = 2,133, p = 0,334). To study the state of free radical oxidation, the method of chemiluminescence (CL) of the oral liquid was applied on the device HL-003. The quantitative and qualitative content of oral microflora was determined using nutrient media: CHROMagar Orientation, CHROMagar Candida (France). The concentration in the oral fluid of the intercellular adhesion molecules sICAM-1 was carried out on the automatic enzyme immunoassay analyzer Lazurit and test systems manufactured by Vector-Best CJSC (Russia) and eBioscience (Austria). Results. The use of the complex proposed by us significantly improved the parameters of the clinical examination (dental indices). In addition, the state of free-radical oxidation has normalized: at previously increased values of the light-emission sum (S) and its maximum value (I max), during the time of the study, their decrease occurred, while the lowered values - the increase to the level of normal values. The level of microorganisms of the orange and red complex decreased to normal levels, and the level of sICAM-1 also significantly decreased. Conclusions. The scheme of treatment of chronic gingivitis with the use of NUTRIEL with laminaria and tooth elixir with chlorophyll in patients with chronic gastroduodenitis led to an increase in the duration of remission compared with the traditional treatment regimen.

scholarly journals Chemiluminescence Studies of Lipoperoxidation Processes in Arterial Hypertension and Stress

2019 ◽  
Vol 4 (1) ◽  
pp. 133-137
Author(s):  
Anastasiya S. Brichagina ◽  
Mariya I. Dolgikh ◽  
Larisa R. Kolesnikova ◽  
Larisa V. Natyaganova
Keyword(s):  
Lipid Peroxidation ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
The State ◽  
Male Rats ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Biochemical Methods ◽  
Peroxidation Processes

Various pathologies, in particular hypertension, accompany violation of the processes of free radical oxidation. The state of the system lipid peroxidation – antioxidant protection can be assessed only by a complex of biochemical methods. The chemiluminescent method is used to obtain a faster result. The method has a number of advantages: measurement of chemiluminescence parameters is carried out in natural conditions and does not require sample preparation, is highly selective, it can be used to identify the presence of free radical pathology, analysis of the intensity of lipid peroxidation processes, the effectiveness of treatment with antioxidant medicament. The aim of the research was to study the lipid peroxidation under hypertension and stress by chemiluminescence. The experiment was conducted on male rats of the ISIAH line. The experiment consisted of 2 stages: stage of alarm and stage of chronic stress. We found that, depending on the type of stress, the state of the system lipid peroxidation-antioxidant protection differed. The role of stress in violation of the processes of lipoperoxidation in hypertension was established. The efficacy of the chemiluminescence method as an alternative to a complex of biochemical methods is shown. Express assessment of pro- and antioxidant status is possible for patients with hypertension.

scholarly journals FEATURES OF CLINICAL AND LABORATORY PARAMETERS AT THE PATIENS WITH RECURRENT ORAL AFTS AND CONDOMITANT ACID-DEPENDENT DISEASES OF THE GASTROINTESTINAL TRACT

2020 ◽  
Vol 16 (3) ◽  
pp. 23-29
Author(s):  
Irina Galimova ◽  
Rita Yunusova ◽  
Irina Usmanova ◽  
Larisa Gerasimova ◽  
Yigal Granot ◽  
...  
Keyword(s):  
Free Radical ◽  
Gastrointestinal Tract ◽  
Oral Cavity ◽  
Oral Hygiene ◽  
Oral Fluid ◽  
Free Radical Oxidation ◽  
The State ◽  
Radical Oxidation ◽  
Laboratory Parameters ◽  
Litmus Paper

Subject. Some features of the clinical picture, hygienic and microbiological state of the oral cavity, acidity and free-radical oxidation of the oral fluid in patients with recurrent aphthates on the background of acid-dependent diseases of the gastrointestinal tract are considered. The aim is to assess the relationship between clinical and laboratory parameters in patients with recurrent oral aphthae associated with acid-dependent diseases of the gastrointestinal tract. Methodology. A comprehensive clinical and laboratory examination of 58 young people with revealed recurrent aphthae of the oral cavity against the background of acid-dependent diseases of the gastrointestinal tract was carried out. The level of oral hygiene was assessed using the ONI-s index, the state of free-radical oxidation of the oral fluid - the "Chemilumimer-003" device, individual representatives of the normal flora were identified by inoculating the contents from the surface of aphthous elements on special differential diagnostic media of the HiCrome series. The acidity of the oral fluid was determined using a universal indicator litmus paper pH-1-14, short-term intragastric pHmetry was performed directly during fibrogastroduodenoscopy. Results. In 46.4 % of those examined, recurrent aphthae were found on the oral mucosa. In persons with chronic gastritis and duodenitis, the frequency of recurrences once a year was 37.9 %, 2-3 times a year ― 62.0, with the presence of chronic pancreatitis against the background of hyperacid gastric secretion ― 44.8 and 55.2, respectively. In patients with recurrent aphthae, a low level of oral hygiene, a violation of its microbiome, and a change in the acidity of the oral fluid were revealed. Conclusions. The presence of recurrent aphthae complicates individual oral hygiene, because accompanied by severe pain and paresthetic symptoms. This is manifested by an increase in the ONI-S index and a disturbance in the state of the normobiome in the form of an increase in the growth of resident microflora against the background of changes in the acidity of the oral fluid. Chemiluminescence indices correlate with the hygienic state of the oral cavity, qualitative changes in the normobiota and pH of the oral fluid in the studied clinical groups.

scholarly journals The role of free radical oxidation and inflammation in the development of post-pericardiotomy syndrome after coronary heart disease artery bypass grafting

2017 ◽  
Vol 94 (11) ◽  
pp. 827-831
Author(s):  
V. V. Vnukov ◽  
Roman V. Sidorov ◽  
N. P. Milyutina ◽  
A. A. Ananyan ◽  
D. Yu. Pospelov ◽  
...  
Keyword(s):  
Free Radical ◽  
Antioxidant System ◽  
Artery Bypass ◽  
Myocardial Revascularization ◽  
Free Radical Oxidation ◽  
Bypass Grafting ◽  
Radical Oxidation ◽  
Artery Bypass Grafting ◽  
Postpericardiotomy Syndrome

The purpose of this research was the clinical biochemical study of the role of the antioxidant system, products of lipid peroxidation and proinflammatory mediators in the mechanisms of development of postpericardiotomy syndrome. 50 patients who underwent coronary-artery bypass grafting were examined. They were divided into two groups: those without postpericardiotomy syndrome (1) and patients with this syndrome (2). The results suggest tension of the antioxidant system, overproduction of proinflammatory interleukin-6 and intensification of free radical oxidation in the patients undergoing direct myocardial revascularization.

scholarly journals Some indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletes

2021 ◽  
Vol 11 (3) ◽  
pp. 12-17
Author(s):  
A. V. Elikov
Keyword(s):  
Superoxide Dismutase ◽  
Free Radical ◽  
Free Radical Oxidation ◽  
The State ◽  
Control Group ◽  
Enzymatic Antioxidants ◽  
Antioxidant Protection ◽  
Radical Oxidation ◽  
Former Athletes ◽  
Oxidative Balance

Objective: to study the main indicators of free radical oxidation and antioxidant protection in the erythrocytes of former athletes, depending on the period of termination of sports.Materials and methods: 24 former male athletes aged 19–29 years were examined, who were divided into 2 groups of 12 people each (1st group — former athletes who stopped training for up to 2 years; 2nd — over 2 years). The control group consisted of 15 practically healthy untrained student volunteers of the same age. In erythrocytes, spectrophotometrically (spectrophotometer Shimadzu 1240, Japan), the activity of antioxidant enzymes was determined: superoxide dismutase (SOD) (K.F. 1.15.1.1) — by inhibiting the reduction of nitro blue tetrazolium by superoxide anion radical at λ = 540 nm, after preliminary processing of erythrocytes by the method of Dubinina E.E. and others [8]; catalase (K.F. 1.11.1.6) — according to the rate of utilization of hydrogen peroxide at λ = 260 nm; glutathione peroxidase (HP) (KF 1.11.1.9) — by the change in the content of reduced glutathione in samples before and after incubation of the substrate with dithiobis-nitrobenzoic acid at λ = 412 nm; glutathione reductase (GR) (K.F. 1.6.4.2) — according to the catalytic NADPH ∙ H+-dependent transformation of the oxidized form of glutathione into the reduced form, the intensity of which was estimated by the rate of decrease in the extinction of samples at λ = 340 nm, at which the NADPH H+ solution has a maximum light absorption (Warburg test).Results: the direction of the shifts in the state of the oxidative balance was established depending on the period of detraining. A significantly lower value of the total antioxidant activity was revealed in the former athletes of the 1st group. This phenomenon is probably associated with a decrease in the efficiency of the enzymatic link of the antioxidant defense in the absence of regular physical exertion, and, as a consequence, an increased consumption of non-enzymatic antioxidants. It was found that the early post-sports period is characterized by significantly lower, in comparison with the control group, the values of the activity of the first-order enzymes (superoxide dismutase) against the background of the increased activity of the second-order enzymes (catalase).Conclusions: the obtained data can be recommended for monitoring the state of athletes who stopped playing sports and taken into account when prescribing rehabilitation measures for the corresponding contingent.

Sign in / Sign up

Export Citation Format

Share Document