scholarly journals ASSOCIATION OF COMPLEX GENOTYPES OF DGAT1, CSN3, LGB GENES WITH ECONOMICALLY VALUABLE SIGNS OF BIRD BULLS

Author(s):  
F.F. Zinnatov ◽  
◽  
T.R. Yakupov ◽  
F.F. Zinnatova ◽  
Ch.A. Kharisova ◽  
...  

Using DNA analysis among the complex genotypes of the DGAT1, CSN3 and LGB genes, it was revealed that 14 different complex genotypes were identified in 63 animals and the highest productivity indices belong to animals with a combination of DGAT1AK CSN3BB LGBBB genotypes – 9261.5 kg and 3.7 % fat and 4,2 % protein. They account for 1.6 % of those studied. Also, cows - mothers with the same combination of genotypes have a high yield of fat and protein – 342.6 kg and 388.9 kg

2019 ◽  
Vol 3 (1) ◽  
pp. 40
Author(s):  
Yanti Ariyanti ◽  
Sister Sianturi

DNA extraction is a series of processes to separate DNA from other cell components. Extraction is the most crucial early stage in molecular research. There are two methods in DNA extraction: traditional organic extraction and non-organic adoption extraction method. Traditional organic extraction method are used by many laboratories to obtain amount of high-yield DNA. However, in recent years it has been a tendency to adopt non-organic commercial protocols, known as extraction kit. This method was relatively faster, low times-consuming and avoid the toxicity due to phenol use. This study aims to determine the steps of DNA extraction to obtain total DNA from muscle tissue of fish grouper using Extraction Kit for Animal Tissue. The Kit extraction method has an advantages that is the processing time is shorter, simple, non-toxic and high-purity DNA yields. A total of 4 tissue samples from grouper muscle were successfully extracted using an Extraction Kit that was indicated by the visualization of the band on qualitative DNA analysis in 1% agarose gel. Keywords: DNA extraction, extraction kit, high-yield DNA, molecular method


2017 ◽  
Vol 51 (05) ◽  
Author(s):  
Hossein Askari ◽  
Seiyed Kamal Kazemitabar ◽  
Hamid Najafi Zarrini ◽  
Mohammd Hossein Saberi

Growing of salt tolerant crops is a good option to obtain economical yields from saline areas and screening salt tolerant plants, particularly in early growth stages as one of the most important determinants of high yield, is important. So a laboratory experiment was performed to assess several indices for identifying barley genotypes with better performance under saline conditions. The effect of salinity stress was studied through an analysis of the dry matter production, that yielding results showed significant differences among genotypes. The multiple statistical procedures which have been used in this study showed that mean productivity and geometric mean productivity indices could be a useful indicator of desirable genotypes in both stress and non-stress conditions at this stage especially in high level of salinity. The majority of used tolerance indices indicated that MBS8715 was the best barley genotype showing the highest stress resistance for the most salt concentration.


Author(s):  
N. Tempel ◽  
M. C. Ledbetter

Carbon films have been a support of choice for high resolution electron microscopy since the introduction of vacuum evaporation of carbon. The desirable qualities of carbon films and methods of producing them has been extensively reviewed. It is difficult to get a high yield of grids by many of these methods, especially if virtually all of the windows must be covered with a tightly bonded, quality film of predictable thickness. We report here a method for producing carbon foils designed to maximize these attributes: 1) coverage of virtually all grid windows, 2) freedom from holes, wrinkles or folds, 3) good adhesion between film and grid, 4) uniformity of film and low noise structure, 5) predictability of film thickness, and 6) reproducibility.Our method utilizes vacuum evaporation of carbon from a fiber onto celloidin film and grid bars, adhesion of the film complex to the grid by carbon-carbon contact, and removal of the celloidin by acetone dissolution. Materials must be of high purity, and cleanliness must be rigorously maintained.


Author(s):  
Hong-Ming Lin ◽  
C. H. Liu ◽  
R. F. Lee

Polyetheretherketone (PEEK) is a crystallizable thermoplastic used as composite matrix materials in application which requires high yield stress, high toughness, long term high temperature service, and resistance to solvent and radiation. There have been several reports on the crystallization behavior of neat PEEK and of CF/PEEK composite. Other reports discussed the effects of crystallization on the mechanical properties of PEEK and CF/PEEK composites. However, these reports were all concerned with the crystallization or melting processes at or close to atmospheric pressure. Thus, the effects of high pressure on the crystallization of CF/PEEK will be examined in this study.The continuous carbon fiber reinforced PEEK (CF/PEEK) laminate composite with 68 wt.% of fibers was obtained from Imperial Chemical Industry (ICI). For the high pressure experiments, HIP was used to keep these samples under 1000, 1500 or 2000 atm. Then the samples were slowly cooled from 420 °C to 60 °C in the cooling rate about 1 - 2 degree per minute to induce high pressure crystallization. After the high pressure treatment, the samples were scanned in regular DSC to study the crystallinity and the melting temperature. Following the regular polishing, etching, and gold coating of the sample surface, the scanning electron microscope (SEM) was used to image the microstructure of the crystals. Also the samples about 25mmx5mmx3mm were prepared for the 3-point bending tests.


Author(s):  
Xin-Ming Xu ◽  
Ming Xie ◽  
Jiazhu Li ◽  
Mei-Xiang Wang

An exquisite Pybox/Cu(OTf)2-catalyzed asymmetric tandem reaction of tertiary enamides was developed, which enabled the expeditious synthesis of indolizino[8,7-b]indole derivatives in high yield, excellent enantioselectivity and diastereoselectivity.


Sign in / Sign up

Export Citation Format

Share Document