scholarly journals Two-step large-volume magnetic separation combined with PCR assay for sensitive detection of Listeria monocytogenes in pasteurized milk

2017 ◽  
Vol 100 (10) ◽  
pp. 7883-7890 ◽  
Author(s):  
Dan Luo ◽  
Xiaolin Huang ◽  
Yan Mao ◽  
Chaochao Chen ◽  
Fulai Li ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Magnetic Separation ◽  
Large Volume ◽  
Sensitive Detection ◽  
Pcr Assay ◽  
Pasteurized Milk

Highly sensitive detection of copper(i) and copper(ii) in cell specimens by CE-UV with large-volume sample stacking

2016 ◽  
Vol 8 (21) ◽  
pp. 4272-4276 ◽  
Author(s):  
Cheng Chen ◽  
Xin Li ◽  
Xia Xie ◽  
Fengxia Chang ◽  
Meixian Li ◽  
...  
Keyword(s):  
Large Volume ◽  
Sensitive Detection ◽  
Sample Stacking ◽  
Highly Sensitive ◽  
Simultaneous Separation ◽  
Sensitive Determination ◽  
Highly Sensitive Detection ◽  
Determination Of Copper ◽  
Large Volume Sample Stacking

A CE-UV method has been developed for the simultaneous separation and sensitive determination of copper(i) and copper(ii) in cell specimens.

scholarly journals Growth kinetics of Salmonella Typhimurium and Listeria monocytogenes in buffalo milk under different processing and storage conditions

2021 ◽  
Vol 51 (11) ◽  
Author(s):  
Joelson Sousa Lima ◽  
Ana Paula Presley Oliveira Sampaio ◽  
Mylla Christy da Silva Dufossé ◽  
Paula Fernanda Morais de Sousa ◽  
Josyane Brasil da Silva ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Salmonella Typhimurium ◽  
Growth Kinetics ◽  
Storage Conditions ◽  
Buffalo Milk ◽  
Production Chain ◽  
Pasteurized Milk ◽  
Processing And Storage ◽  
And Storage ◽  
Kinetics Of

ABSTRACT: Buffalo milk is rich in nutrients and can serve as a substrate for the proliferation of microorganisms. Thus, the objective of the present study was to evaluate the growth kinetics of Salmonella Typhimurium and Listeria monocytogenes in buffalo milk under different processing and storage conditions. Samples of raw and pasteurized milk were inoculated with 1 CFU of each bacterium, separately and together, per 25 mL. After contamination, samples were stored at 8 °C or 37 °C, and bacterial counts were performed at 24, 48, and 168 h. In addition, the accompanying microbiota growth, pH, and the effect of these variables on the growth kinetics of microorganisms were monitored. The pathogens tested were able to proliferate under most conditions tested, reaching high titers throughout the experimental period. At 37 °C, there was a decrease in pH and an increase in the accompanying microbiota that interfered with the microbial growth curve. It was also observed that pasteurized milk subjected to 8 °C provided better conditions for the multiplication of bacteria. Therefore, it was concluded that care throughout the production chain, storage, and commercialization of milk must be adopted to guarantee the microbiological safety of this food.

scholarly journals Evaluation of sanitary quality of goat milk in dairy industries from the Cariri region, state of Paraíba

2021 ◽  
Vol 10 (12) ◽  
pp. e596101220735
Author(s):  
Iara Nunes de Siqueira ◽  
Aline Antas Cordeiro Cavalcanti ◽  
Joyce Galvão de Souza ◽  
Filipe Jordão Pereira de Medeiros ◽  
João Carlos Taveira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Pathogenic Bacteria ◽  
Goat Milk ◽  
Salmonella Spp ◽  
Pasteurized Milk ◽  
Enterococcus Spp ◽  
Bacillus Spp ◽  
Thermotolerant Coliforms ◽  
Staphylococcus Spp

The sanitary evaluation of equipment and hands is fundamental to investigate the presence of pathogens in the dairy industry. Then, this study aims to evaluate the sanitization of equipment, workers’ hands, raw and pasteurized milk in goat milk dairies in the Cariri region, state of Paraíba.  Collected 32 samples of four dairies represented by letters A, B, C, and D. The followings contents were analyzed: mesophiles, total and thermotolerant coliforms, Escherichia coli, Staphylococcus aureus, Samonella spp. and Listeria monocytogenes in the reception tank, pasteurization tank, packing machine, package, wall, workers’ hand, and each dairy’s raw and pasteurized milk. After isolation, 84 colonies were confirmed by MALDI TOF. The indicator microorganisms presented variations for the workers’ hands, while A and B stayed within the patterns. For the equipment, only dairy B was within limits. They were out of the standard for mesophiles, total coliforms, and thermotolerant regarding raw and pasteurized milk. The microorganisms, the Enterobacteriaceae family presented a higher frequency, with 77.38%, and within this family, Escherichia coli, Klebsiella spp., and Enterobacter spp. were the most prevalent. Gram-positive corresponded to 22.62%, Bacillus spp., Staphylococcus spp., Enterococcus spp., and Macrococcus caseolyticus. Listeria monocytogenes and Salmonella spp. were not isolated. These demonstrate failures in goat milk processing with pathogenic bacteria in several dairy plants, indicating the need to adjust the product’s quality control.

Rapid and Sensitive Detection of Major Uropathogens in a Single-Pot Multiplex PCR Assay

2012 ◽  
Vol 65 (1) ◽  
pp. 44-53 ◽  
Author(s):  
B. Padmavathy ◽  
R. Vinoth Kumar ◽  
Amee Patel ◽  
S. Deepika Swarnam ◽  
T. Vaidehi ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Sensitive Detection ◽  
Pcr Assay ◽  
Multiplex Pcr Assay

Radial ZnO Nanorods Decorating Co3O4 Nanoparticles for Highly Selective and Sensitive Detection of 3-Hydroxy-2-Butanone Biomarker

Nanoscale ◽  
2021 ◽  
Author(s):  
Chen Wang ◽  
Lingling Du ◽  
Xiaxia Xing ◽  
Dongliang Feng ◽  
Yingying Tian ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Gas Sensor ◽  
Zno Nanorods ◽  
High Sensitivity ◽  
Sensitive Detection ◽  
Co3o4 Nanoparticles

Indirectly monitoring Listeria monocytogenes (LMs) via gas sensor detecting metabolites 3-hydroxy-2-butanone (3H-2B) biomarker is a newly-emerged strategy. However, such sensors are required to simultaneously endow with outstanding selectivity, high sensitivity...

Development of multiplex PCR assay for rapid detection Listeria monocytogenes in clinical samples

2021 ◽  
Vol 30 (4) ◽  
pp. 20-26
Author(s):  
Le Thanh Huong ◽  
Ha Thi Phuong Mai ◽  
Hoang Thi Thu Ha ◽  
Nguyen Dong Tu ◽  
Bui Tien Sy ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Multiplex Pcr ◽  
Rapid Detection ◽  
Pathogenic Bacteria ◽  
Vulnerable Groups ◽  
Clinical Samples ◽  
Specific Gene ◽  
Pcr Assay ◽  
Multiplex Pcr Assay ◽  
Specificity And Sensitivity

Listeria monocytogenes is widely present in the natural environment. This bacteria can cause infections in both humans and animals. In humans, the most vulnerable groups to be infected with L. monocytogenes are the elderly, people with an impaired immune system and chronically illness, pregnant women, and newborn babies. The aim of this study was to develop a multiplex PCR assay for the rapid detection of L. monocytogenes in mock clinical samples. A pair of primers were designed for detection of L. monocytogenes based on prs, a Listeria genus specific gene, and hly, a hemolysin gene. The specificity of the primers were tested by using different L. monocytogenes strains and other common pathogenic bacteria. The results showed that L. monocytogenes strains were positive in the detection and other tested strains were negative in mock (spiked) clinical samples. The sensitivity of multiplex PCR assay was 102 CFU/ml per reaction. The specificity and sensitivity of multiplex PCR technology for detecting L. monocytogenes in mock (spiked) clinical samples were high, and the assay could be completed within 1.5 hours. Therefore, this established multiplex PCR provides a rapid and reliable method and will be useful for the detection of L. monocytogenes in mock clinical samples.

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