Effect of camel milk protein hydrolysates against hyperglycemia, hyperlipidemia, and associated oxidative stress in streptozotocin (STZ)-induced diabetic rats

Purpose The purpose of the present study was to investigate the effect of camel milk protein hydrolysates (CMPHs) on physico-chemical, sensory, colour profile and textural quality attributes of chevon patties. Design/methodology/approach Camel milk proteins were hydrolyzed with three different proteolytic enzymes, viz., alcalase (CMPH-A), α-chymotrypsin (CMPH-C) and papain (CMPH-P), and dried to powder form before further utilization. Four treatments were prepared with incorporation of CMPH, viz., CMPH 0 per cent (C), CMPH-A 0.09 per cent (T1), CMPH-C 0.06 per cent (T2) and CMPH-P 0.09 per cent (T3), in the product formulation. The developed goat meat patties were evaluated for physico-chemical (pH; emulsion stability, ES; cooking yield, CY; water activity, aw), instrumental colour and texture profile and sensory attributes. Findings The pH, moisture, fat and ES values of goat meat emulsions were comparable amongst treatments as well as with the control; however, treated emulsions had higher ES and moisture content. The pH and moisture per cent of cooked chevon patties varied significantly, whereas other physico-chemical (CY, aw, per cent protein, per cent fat, per cent ash and per cent dietary fibre) as well as dimensional parameters (per cent gain in height and decrease in diameter) were comparable amongst treatments and the control. Hardness, springiness, stringiness, cohesiveness, gumminess and resilience of chevon patties decreased significantly (p < 0.05) with the incorporation of CMPH than that of the control; however, the values were comparable among all the treated products. Protein hydrolysate in chevon patties resulted in significant increase in redness (a*) values, whereas all other parameters (L*, b* and hue) decreased significantly as compared to that of the control. The colour and appearance, texture, juiciness overall acceptability scores were comparable in all the treated products and were significantly (p < 0.05) higher than the control. The flavour scores of C, T1 and T3 were comparable but significantly lower than that of T2. The overall acceptability scores of T1 and T2 were also comparable and significantly higher than C and T3; however, the highest score was recorded for T2. Practical implications Results concluded that chevon patties with acceptable sensory attributes and improved CY and textural attributes can be successfully developed with the incorporation of CMPH. Originality/value The protein hydrolysates of different food proteins could be explored in a same pattern to find out their implication in food matrices.

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Camel milk protein hydrolysates with improved technofunctional properties and enhanced antioxidant potential in in vitro and in food model systems

Journal of Dairy Science ◽

10.3168/jds.2017-13194 ◽

2018 ◽

Vol 101 (1) ◽

pp. 47-60 ◽

Cited By ~ 26

Author(s):

Kholoud Awad Al-Shamsi ◽

Priti Mudgil ◽

Hassan Mohamed Hassan ◽

Sajid Maqsood

Keyword(s):

Milk Protein ◽

Protein Hydrolysates ◽

Model Systems ◽

Antioxidant Potential ◽

Camel Milk ◽

Food Model

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Molecular binding mechanism and identification of novel anti-hypertensive and anti-inflammatory bioactive peptides from camel milk protein hydrolysates

LWT ◽

10.1016/j.lwt.2019.05.091 ◽

2019 ◽

Vol 112 ◽

pp. 108193 ◽

Cited By ~ 8

Author(s):

Priti Mudgil ◽

Bincy Baby ◽

Ying-Yuan Ngoh ◽

Hina Kamal ◽

Ranjit Vijayan ◽

...

Keyword(s):

Milk Protein ◽

Bioactive Peptides ◽

Protein Hydrolysates ◽

Camel Milk ◽

Binding Mechanism ◽

Molecular Binding ◽

Anti Inflammatory

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Dipeptidyl peptidase IV (DPP-IV) inhibitory properties of camel milk protein hydrolysates generated with trypsin

Journal of Functional Foods ◽

10.1016/j.jff.2017.04.016 ◽

2017 ◽

Vol 34 ◽

pp. 49-58 ◽

Cited By ~ 35

Author(s):

Alice B. Nongonierma ◽

Sara Paolella ◽

Priti Mudgil ◽

Sajid Maqsood ◽

Richard J. FitzGerald

Keyword(s):

Milk Protein ◽

Dipeptidyl Peptidase ◽

Protein Hydrolysates ◽

Dipeptidyl Peptidase Iv ◽

Camel Milk ◽

Dpp Iv

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Characterization and identification of novel antidiabetic and anti-obesity peptides from camel milk protein hydrolysates

Food Chemistry ◽

10.1016/j.foodchem.2018.03.082 ◽

2018 ◽

Vol 259 ◽

pp. 46-54 ◽

Cited By ~ 37

Author(s):

Priti Mudgil ◽

Hina Kamal ◽

Gan Chee Yuen ◽

Sajid Maqsood

Keyword(s):

Milk Protein ◽

Protein Hydrolysates ◽

Camel Milk

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Identification of novel dipeptidyl peptidase IV (DPP-IV) inhibitory peptides in camel milk protein hydrolysates

Food Chemistry ◽

10.1016/j.foodchem.2017.10.033 ◽

2018 ◽

Vol 244 ◽

pp. 340-348 ◽

Cited By ~ 41

Author(s):

Alice B. Nongonierma ◽

Sara Paolella ◽

Priti Mudgil ◽

Sajid Maqsood ◽

Richard J. FitzGerald

Keyword(s):

Milk Protein ◽

Dipeptidyl Peptidase ◽

Protein Hydrolysates ◽

Dipeptidyl Peptidase Iv ◽

Camel Milk ◽

Inhibitory Peptides ◽

Dpp Iv

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Attenuation of oxidative stress in hepatic and pancreatic tissues of STZ-induced diabetic rats treated with aqueous extract of Vochysia rufa

Planta Medica ◽

10.1055/s-0034-1394539 ◽

2014 ◽

Vol 80 (16) ◽

Cited By ~ 2

Author(s):

NM De Gouveia ◽

IB Moraes ◽

RMF Sousa ◽

MB Neto ◽

AV Mundim ◽

...

Keyword(s):

Oxidative Stress ◽

Aqueous Extract ◽

Diabetic Rats

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Testicular Injury Attenuated by Rapamycin Through Induction of Autophagy and Inhibition of Endoplasmic Reticulum Stress in Streptozotocin- Induced Diabetic Rats

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1871530319666190102112844 ◽

2019 ◽

Vol 19 (5) ◽

pp. 665-675 ◽

Cited By ~ 4

Author(s):

Wenjiao Shi ◽

Zhixin Guo ◽

Ruixia Yuan

Keyword(s):

Oxidative Stress ◽

Endoplasmic Reticulum ◽

Endoplasmic Reticulum Stress ◽

Protective Effect ◽

Er Stress ◽

B Cell Lymphoma ◽

Diabetic Rats ◽

Pathological Changes ◽

Rapamycin Treatment ◽

Related Proteins

Background and Objective: This study investigated whether rapamycin has a protective effect on the testis of diabetic rats by regulating autophagy, endoplasmic reticulum stress, and oxidative stress. Methods: Thirty male Sprague-Dawley rats were randomly divided into three groups: control, diabetic, and diabetic treated with rapamycin, which received gavage of rapamycin (2mg.kg-1.d-1) after induction of diabetes. Diabetic rats were induced by intraperitoneal injection of streptozotocin (STZ, 65mg.Kg-1). All rats were sacrificed at the termination after 8 weeks of rapamycin treatment. The testicular pathological changes were determined by hematoxylin and eosin staining. The protein or mRNA expression of autophagy-related proteins (Beclin1, microtubule-associated protein light chain 3 (LC3), p62), ER stress marked proteins (CCAAT/enhancer-binding protein (C/EBP) homologous protein (CHOP), caspase-12), oxidative stress-related proteins (p22phox, nuclear factor erythroid2-related factor 2 (Nrf2)) and apoptosis-related proteins (Bax, B cell lymphoma-2 (Bcl-2)) were assayed by western blot or real-time fluorescence quantitative PCR. Results: There were significant pathological changes in the testes of diabetic rats. The expression of Beclin1, LC3, Nrf2, Bcl-2 were significantly decreased and p62, CHOP, caspase12, p22phox, and Bax were notably increased in the testis of diabetic rats (P <0.05). However, rapamycin treatment for 8 weeks significantly reversed the above changes in the testis of diabetic rats (P <0.05). Conclusion: Rapamycin appears to produce a protective effect on the testes of diabetic rats by inducing the expression of autophagy and inhibiting the expression of ER-stress, oxidative stress, and apoptosis.

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Exercise and Stevia Rebaudiana (R) Extracts Attenuate Diabetic Cardiomyopathy in Type 2 Diabetic Rats: Possible Underlying Mechanisms

Endocrine Metabolic & Immune Disorders - Drug Targets ◽

10.2174/1871530320666200420084444 ◽

2020 ◽

Vol 20 (7) ◽

pp. 1117-1132

Author(s):

Abdelaziz M. Hussein ◽

Elsayed A. Eid ◽

Ismaeel Bin-Jaliah ◽

Medhat Taha ◽

Lashin S. Lashin

Keyword(s):

Oxidative Stress ◽

Blood Glucose ◽

Diabetic Cardiomyopathy ◽

Caspase 3 ◽

Stevia Rebaudiana ◽

Diabetic Rats ◽

Control Group ◽

Underlying Mechanisms ◽

Type 2 Diabetic

Background and Aims: In the current work, we studied the effects of exercise and stevia rebaudiana (R) extracts on diabetic cardiomyopathy (DCM) in type 2 diabetic rats and their possible underlying mechanisms. Methods: : Thirty-two male Sprague Dawley rats were randomly allocated into 4 equal groups; a) normal control group, b) DM group, type 2 diabetic rats received 2 ml oral saline daily for 4 weeks, c) DM+ Exercise, type 2 diabetic rats were treated with exercise for 4 weeks and d) DM+ stevia R extracts: type 2 diabetic rats received methanolic stevia R extracts. By the end of the experiment, serum blood glucose, HOMA-IR, insulin and cardiac enzymes (LDH, CK-MB), cardiac histopathology, oxidative stress markers (MDA, GSH and CAT), myocardial fibrosis by Masson trichrome, the expression of p53, caspase-3, α-SMA and tyrosine hydroxylase (TH) by immunostaining in myocardial tissues were measured. Results: T2DM caused a significant increase in blood glucose, HOMA-IR index, serum CK-MB and LDH, myocardial damage and fibrosis, myocardial MDA, myocardial α-SMA, p53, caspase-3, Nrf2 and TH density with a significant decrease in serum insulin and myocardial GSH and CAT (p< 0.05). On the other hand, treatment with either exercise or stevia R extracts significantly improved all studied parameters (p< 0.05). Moreover, the effects of stevia R was more significant than exercise (p< 0.05). Conclusion: Both exercise and methanolic stevia R extracts showed cardioprotective effects against DCM and Stevia R offered more cardioprotective than exercise. This cardioprotective effect of these lines of treatment might be due to attenuation of oxidative stress, apoptosis, sympathetic nerve density and fibrosis and upregulation of the antioxidant transcription factor, Nrf2.

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Eudragit L-100 Capsules Aromatize and Quaternerize Chitosan for Insulin Nanoparticle Oral Delivery During Toxic Oxidative Stress in Rat Liver and Kidney

Pharmaceutical Nanotechnology ◽

10.2174/2211738508666200628033442 ◽

2020 ◽

Vol 8 (3) ◽

pp. 239-254 ◽

Cited By ~ 2

Author(s):

Reza Mahjub ◽

Farzane K. Najafabadi ◽

Narges Dehkhodaei ◽

Nejat Kheiripour ◽

Amir N. Ahmadabadi ◽

...

Keyword(s):

Oxidative Stress ◽

Oral Administration ◽

Oral Delivery ◽

Biochemical Parameters ◽

Kidney Injury ◽

Regular Insulin ◽

Treated Group ◽

Histopathological Change ◽

Diabetic Rats ◽

Liver And Kidney

Background: Insulin, like most peptides, is classified as a hydrophilic and macromolecular drug that is considered as a low permeable and unstable compound in the gastrointestinal (GI) tract. The acidic condition of the stomach can degrade insulin molecules. Moreover, the presence of proteolytic activities of some enzymes such as trypsin and chymotrypsin can hydrolyze amide-bonds between various amino-acids in the structures of peptides and proteins. However, due to its simplicity and high patient compliance, oral administration is the most preferred route of systemic drug delivery, and for the development of an oral delivery system, some obstacles in oral administration of peptides and proteins including low permeability and low stability of the proteins in GI should be overcome. Objective: In this study, the effects of orally insulin nanoparticles (INPs) prepared from quaternerized N-aryl derivatives of chitosan on the biochemical factors of the liver in diabetic rats were studied. Methods: INPs composed of methylated (amino benzyl) chitosan were prepared by the PEC method. Lyophilized INPs were filled in pre-clinical capsules, and the capsules were enteric-coated with Eudragit L100. Twenty Male Wistar rats were randomly divided into four groups: group1: normal control rats, group 2: diabetic rats, group 3: diabetic rats received capsules INPs(30 U/kg/day, orally), group 4: the diabetic rats received regular insulin (5 U/kg/day, subcutaneously). At the end of the treatment, serum, liver and kidney tissues were collected. Biochemical parameters in serum were measured using spectrophotometric methods. Also, oxidative stress was measured in plasma, liver and kidney. Histological studies were performed using H and E staining . Results: Biochemical parameters, and liver and kidney injury markers in serum of the diabetic rats that received INPs improved significantly compared with the diabetic group. INPs reduced oxidative toxic stress biomarkers in serum, liver and kidney of the diabetic treated group. Furthermore, a histopathological change was developed in the treated groups. Conclusion: Capsulated INPs can prevent diabetic liver and oxidative kidney damages (similar regular insulin). Therefore oral administration of INPs appears to be safe. Lay Summary: Although oral route is the most preferred route of administration, but oral delivery of peptides and proteins is still a challenging issue. Diabetes Mellitus may lead to severe complications, which most of them are life-threatening. In this study, we are testing the toxicity of oral insulin nanoparticles in kidney and liver of rats. For this investigation, we will prepare insulin nanoparticles composed of a quaternized derivative of chitosan. The nanoparticles will be administered orally to rats and the level of oxidative stress in their liver and kidney will be determined. The data will be compared to the subcutaneous injection of insulin.

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