scholarly journals Substitution of Filter Paper for Crucibles in the in Vitro Rumen True Digestibility Determination

1972 ◽  
Vol 55 (9) ◽  
pp. 1305-1307 ◽  
Author(s):  
J.B. Robertson ◽  
P.J. Van Soest ◽  
Filemon Torres
Keyword(s):  
Filter Paper ◽  
True Digestibility

PERBANDINGAN DAYA HAMBAT BAKTERI Pseudomonas fluorescens ASAL TOMAT, KEDELAI, DAN JAGUNG TERHADAP Ralstonia solanacearum SECARA IN VITRO

Agrotek ◽  
2018 ◽  
Vol 5 (6) ◽  
Author(s):  
Aco Roni Kirihio ◽  
Ivonne Fitria Mariay ◽  
Cipta Meliala
Keyword(s):  
Pseudomonas Fluorescens ◽  
Filter Paper ◽  
Ralstonia Solanacearum ◽  
Randomized Design ◽  
Completely Randomized Design

<em>Inhibition of Pseudomonas fluorescens isolates the origin of tomato, soybean and corn against Ralstonia solanacearum tested using a completely randomized design (CRD).        P. fluorescens growth was measured at King's B medium by way of suspension antagonist put on filter paper of 0.5 cm in diameter. Inhibition of P. fluorescens is done by placing the antagonist suspension of 0.5 cm diameter filter paper on NA media that has been deployed R. solanacearum. The results showed that: (a) the growth of P. fluorescens origin of tomato, soybean and corn on King's B media were not significantly different, (b) the inhibition of P. fluorescens isolates against R. solanacearum not significantly different and, (c) the inhibition of isolates P. fluorescens origin of tomato, soybean and corn against R. solanacearum in vitro relatively strong</em>

EFFECT OF STRUCTURAL AND CHEMICAL FACTORS OF FORAGES ON POTENTIALLY DIGESTIBLE FIBER, INTAKE, AND TRUE DIGESTIBILITY BY RUMINANTS

1988 ◽  
Vol 68 (3) ◽  
pp. 787-799 ◽  
Author(s):  
V. GIRARD ◽  
G. DUPUIS
Keyword(s):  
Cell Wall ◽  
Dry Matter ◽  
Plant Cell Wall ◽  
Neutral Detergent Fiber ◽  
Optimum Number ◽  
Apparent Digestibility ◽  
Cool Season ◽  
Dry Matter Digestibility ◽  
True Digestibility

In view of the large variation found in plant cell wall digestibilities with ruminants, an attempt was made to group 124 feeds into different lignification classes (clusters) on the basis of chemical characteristics. Each feed cluster was described using a structural coefficient [Formula: see text] that related the potentially digestible fiber (PDF, %) to the ratio between lignin and cell wall volume. The optimum number of clusters was determined iteratively by performing a regression of the apparent digestibility of dry matter at maintenance level (DDM1, %) against the PDF and cell soluble (SOL, %) contents of feeds. The [Formula: see text] coefficients varied from 0.05 (grains, N = 13) to 1.85 (corn silage, N = 3) and increased with the maturity of the grasses from 0.88 (legumes, vegetative cool season grasses, N = 26) to 1.33 (mature, cool season grasses, N = 19). Predicted PDF were closely correlated (r > 0.9, P < 0.01) to in vitro cell wall disappearances (IVCWD). Apparently digestible cell wall in four grasses and four legumes increased linearly with 96-h IVCWD and standard error (SE) was similar to the SE of predicted apparent digestible SOL from SOL concentrations. Assuming that similarity between SE could be also observed in larger samples, PDF and SOL were used in summative equations to predict apparent dry matter digestibility. DDM1 discounted for intake (DDM1 – 4, %) was regressed against SOL and PDF concentrations of 87 feeds:[Formula: see text]with ds and df, the true digestibilities of SOL and PDF. Estimates of ds and df were 0.98 and 0.95 for a zero-production (maintenance) level of intake, and 0.91 and 0.79 for an intake level four times maintenance. Since the true digestibility of the PDF component was only 4% – 13% lower than that of the cell soluble component, the concentration of PDF in cell wall was the major determinant in the variation in apparent digestibility of forages. Key words: lignin, neutral detergent fiber, true digestibility, cluster analysis, feeds

Filter paper-based 3D liver model in vitro drug screening applications

2018 ◽  
Vol 44 ◽  
pp. S110
Author(s):  
T. Agarwal ◽  
T. Maiti ◽  
S. Ghosh
Keyword(s):  
Drug Screening ◽  
Filter Paper ◽  
Liver Model ◽  
In Vitro Drug Screening

scholarly journals In Vitro Seed Germination and Embryo Culture in Nothapodytes foetida (Wight) Sleumer

2015 ◽  
Vol 48 ◽  
pp. 23-31 ◽  
Author(s):  
S. Kaveri ◽  
Rao Srinath
Keyword(s):  
Seed Germination ◽  
Embryo Culture ◽  
Filter Paper ◽  
Agar Medium ◽  
Ms Medium ◽  
Mature Embryos ◽  
Nothapodytes Foetida ◽  
Half Strength ◽  
Paper Bridge

In vitro seed germination and embryo culture have been achieved in Nothapodytes foetida, this plant is known for its rich source of anticancer drug i. e., Camptothecin. In present study both normal and decoated seeds were subjected to different treatments viz., H2O, GA3, H2O2, H2SO4, chlorine water and mechanical scarification, further these were germinated on water agar medium (WA), filter paper bridge (FB), half strength MS (HMS) and full strength MS (FMS) medium. The highest percentage (69%) of germination was achieved from decoated seeds treated with 10mg/L GA3 and germinated on Filter Paper Bridge. And for embryo culture mature embryos were inoculated on MS medium containing various combination and concentrations of cytokinins (BAP, Kn and TDZ) and auxin (IAA and NAA) for rapid conversion into a plantlet. Among the different combinations of growth regulators; highest frequency (100%) of plantlet conversion was obtained on MS medium containing Kn (1.0mg/L) and NAA (0.2mg/L).

scholarly journals PHAGOCYTOSIS OF BACTERIA IN THE ABSENCE OF ANTIBODY AND THE EFFECT OF PHYSICAL SURFACE

1956 ◽  
Vol 104 (2) ◽  
pp. 233-243 ◽  
Author(s):  
Edwin M. Lerner
Keyword(s):  
Filter Paper ◽  
Chemical Effect ◽  
Type I ◽  
Blood Leukocytes ◽  
Experimental Conditions ◽  
Phagocytic Capacity ◽  
E Coli ◽  
Physical Surface ◽  
Type Iv

The present experiments have shown that phagocytosis occurs in the absence of specific antibody and in the absence of a "suitable physical surface", as further that the presence of a rough surface does not increase the in vitro phagocytosis of pneumococci by polymorphonuclear leukocytes. This held true during repetition of Wood's experiments, as well as when more controlled quantitative techniques were employed, when conditions were made optimal for phagocytosis by increasing bacterial concentrations, and when blood leukocytes were substituted for exudate leukocytes. Evidence has been presented previously that the stimulation of phagocytosis of E. coli, B. abortus, and Type IV Pneumococcus, after contact with filter paper or an active compound present in filter paper, is a chemical effect rather than a physical effect. This type of stimulation did not occur with the Type I A5 Pneumococcus. The leukocyte of the circulating blood was found to be definitely superior to the exudate leukocyte in phagocytic capacity, under all the experimental conditions tested.

Improved Routine Maintenance of Nippostrongylus brasiliensis Culture

1968 ◽  
Vol 42 (1-2) ◽  
pp. 193-198
Author(s):  
Paul Whur
Keyword(s):  
Paper Chromatography ◽  
Filter Paper ◽  
Fungal Growth ◽  
Petri Dish ◽  
Nippostrongylus Brasiliensis ◽  
Total Inhibition ◽  
Culture Maintenance ◽  
Time Required

A new method is described for the in vitro culture of the preparasitic stages of Nippostrongylus brasiliensis. It utilises the plating of faecal suspensions on to paper chromatography strips supported on non-porous Perspex slabs and has been designed primarily to facilitate savings in the time required for routine culture maintenance without lowering the quantity or quality of the larval yield. Comparison with the filter paper/Petri dish method shows an increase in larval yield of 85% and a reduction in time required for culture of 72%. Total inhibition of fungal growth on incubated faeces suspensions was obtained by the addition of “Mycostatin” (Squibb) in a concentration of 62 units per ml. or more.

552 Effects of fibrolytic enzyme on in-vitro true digestibility of by-products commonly fed to cattle

2017 ◽  
Vol 95 (suppl_4) ◽  
pp. 270-270
Author(s):  
L. Barbosa Kondratovich ◽  
J. O. Sarturi ◽  
M. A. Ballou ◽  
D. Sugg ◽  
P. R. B. Campanili ◽  
...  
Keyword(s):  
True Digestibility ◽  
By Products

Leaf and stem nutritive value of timothy genotypes under varying N fertilization

2004 ◽  
Vol 84 (4) ◽  
pp. 1101-1104 ◽  
Author(s):  
G. Bélanger ◽  
A. Brégard ◽  
R. Michaud ◽  
G. F. Tremblay
Keyword(s):  
Cell Wall ◽  
Nutritive Value ◽  
N Fertilization ◽  
Phleum Pratense ◽  
Neutral Detergent Fiber ◽  
Acid Detergent Fiber ◽  
True Digestibility ◽  
Sib Families ◽  
Phleum Pratense L

Populations and half-sib families of timothy (Phleum pratense L.), contrasting in forage N concentration, were studied in two experiments conducted in growth rooms with varying N applications. They differed in leaf and stem in vitro true digestibility (IVTD) of DM and in vitro cell wall digestibility (IVCWD), and in stem neutral detergent fiber (NDF) concentration; this confirms the presence of genetic variability for leaf and stem nutritive value in timothy under both limiting and non-limiting N conditions. Key words: Digestibility, grasses, Phleum pratense, neutral detergent fiber, acid detergent fiber

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