The Effect of Preservatives Based on Propionic Acid on the Fermentation and Aerobic Stability of Corn Silage and a Total Mixed Ration

We aimed to evaluate nutritional quality, fermentation profile, aerobic stability, and dry matter losses in corn (Zea mays) and wheat (Triticum aestivum 'BRS Umbu') silages. Treatments included uninoculated and inoculated (Lactobacillus plantarum and Pediococcus acidilactici, 1.0 × 105 UFC g-1) wheat silage, corn silage from a conventional hybrid and a transgenic hybrid. Nutritional quality and fermentation profile variables were tested in a completely randomized design. Means were compared using Tukey’s test at 5% significance. An aerobic stability trial was conducted in a factorial design with two silages (wheat × inoculated wheat; conventional hybrid corn × transgenic hybrid corn) and two temperatures (ambient temperature × controlled temperature at 24°C). Data were submitted to ANOVA and means were analyzed by the F test at 5% probability. Inoculation of wheat silage increased dry matter, organic matter, and total carbohydrates, but reduced crude protein by a dilution effect. Regarding the fermentation profile, inoculation reduced acetic acid and butyric acid content, whereas it increased propionic acid in wheat silage. Bt corn hybrid silage showed higher dry matter and lower neutral detergent fiber, whereas transgenic corn silage showed lower content of acetic acid, propionic acid, alcohol, and ammonia. Conversely, Bt hybrid silage showed higher butyric acid. Transgenic corn silage showed higher temperature than the conventional hybrid silage during aerobic exposure. Inoculated wheat silage experienced larger deterioration and dry matter losses during the aerobic stability trial. Temperature control worsened aerobic stability in all treatments, increasing dry matter losses and heating.

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Effect of lactic acid bacteria and propionic acid on conservation characteristics, aerobic stability and in vitro gas production kinetics and digestibility of whole-crop corn based total mixed ration silage

Journal of Integrative Agriculture ◽

10.1016/s2095-3119(16)61482-x ◽

2017 ◽

Vol 16 (7) ◽

pp. 1592-1600 ◽

Cited By ~ 8

Author(s):

Lei CHEN ◽

Xian-jun YUAN ◽

Jun-feng LI ◽

Si-ran WANG ◽

Zhi-hao DONG ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Propionic Acid ◽

Gas Production ◽

Total Mixed Ration ◽

Production Kinetics ◽

Aerobic Stability ◽

In Vitro Gas Production

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Evaluation of Fermentation and Aerobic Stability of Total Mixed Ration Silage Containing Wet Brewers’ Grains and Corn Straw

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.347-353.189 ◽

2011 ◽

Vol 347-353 ◽

pp. 189-192

Author(s):

Hui Li Wang ◽

Qi Zhong Sun ◽

Fu Yu Yang ◽

Chun Cheng Xu

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Propionic Acid ◽

Air Temperature ◽

Heat Production ◽

Lactic Acid Concentration ◽

Corn Straw ◽

Total Mixed Ration ◽

Aerobic Stability ◽

Fermentation Quality

This experiment was conducted to evaluate the effect of ensiling on fermentation quality and aerobic stability of a total mixed ration (TMR) containing wet brewers’ grains and corn straw. During the ensiling period, pH fell dramatically from 6.00 to 3.92 at the initial 3 days, then it maintained relatively stable. Lactic acid concentration firstly increased rapidly then it became slowly to reach 3.21% at day 28 post-ensiling. No propionic acid or butyric acid was observed throughout the ensiling. When exposed to air, the temperature of TMR increased quickly to reach a maximum of about 45°C at the 6-day, then it tended to decline until day 9. Later, it had another relative low peak at the 10-day, then it dropped slowly to be equal to air temperature. For TMR silage, no heat production or mold were detected in the entire period. In addition, during the days of exposure, the pH for TMR varied from 6.0-8.7, while TMR silages had no significant differences (3.86 to 3.87). The number of lactic acid bacteria (LAB) for the TMR decreased from the initial 3.2×103cfu g-1to below detectable levels and yeast counts increased by 1000 times. However, the TMR silage had no significant change in LAB and yeast counts. These results indicated that the TMR silage showed great quality and aerobic stability. Overall, fermentation plays an important part in helping total mixed ration silage forming a good aerobic stability.

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Effect of 11CFT and 11C33 inoculants on the chemical and fermentation composition, and aerobic stability of corn silage during the feed out period

Semina Ciências Agrárias ◽

10.5433/1679-0359.2021v42n1p395 ◽

2021 ◽

pp. 395-410

Author(s):

Tânia Mara Becher Ribas ◽

◽

Mikael Neumann ◽

Egon Henrique Horst ◽

Fernando Braga Cristo ◽

...

Keyword(s):

Acetic Acid ◽

Lactic Acid ◽

Experimental Design ◽

Lactic Acid Bacteria ◽

Propionic Acid ◽

Dry Matter ◽

Corn Silage ◽

Bacterial Inoculants ◽

Aerobic Stability

The objective was to evaluate the efficiency of two bacterial inoculants, 11CFT and 11C33, with different genera of lactic acid bacteria on the chemical and fermentation composition of the silage, and the temperature and pH behavior of the silage during the feed out period. The experimental design used was randomized blocks, with three treatments: corn silage without inoculant (control); corn silage with 11CFT inoculant (consisting of strains of Lactobacillus buchneri and L. casei); and corn silage with 11C33 inoculant (consisting of strains of L. buchneri, L. plantarum and Enterococcus faecium). The use of both inoculants increased the concentration of lactic acid in the silage (22.42 g kg-1 for control against 36.00 and 33.33 g kg-1 for 11CFT and 11C33, respectively) and reduced aerobic dry matter losses. The silage treated with 11C33 obtained a higher concentration of acetic acid (17.44 g kg-1) and propionic acid (2.08 g kg-1). The 11CFT inoculant provided a lower concentration of ethanol, however, without differing from the silage with 11C33 (0.70 and 1.61 g kg-1, respectively). Even without variations in temperature and pH at silage unloading, the use of the 11C33 inoculant generated a higher concentration of acetic and propionic acid, providing better aerobic stability days after unloading. Both inoculants also improved the in situ ruminal digestibility of corn silage compared to control silage. They provide an increase in the content of lactic and propionic acids, which assist to reduce dry matter losses and ethanol production. There were no variations in temperature and pH at the silo unloading, however, the use of the 11C33 inoculant generated a higher concentration of acetic and propionic acids providing better aerobic stability after exposure to air.

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Evaluating fermentation quality, in vitro digestibility and aerobic stability of a total mixed ration ensiled with different additives on Tibet plateau

Animal Bioscience ◽

10.5713/ajas.19.0972 ◽

2021 ◽

Vol 34 (2) ◽

pp. 223-232

Author(s):

Zhihao Dong ◽

Siran Wang ◽

Jie Zhao ◽

Junfeng Li ◽

Qinhua Liu ◽

...

Keyword(s):

Acetic Acid ◽

Tibetan Plateau ◽

Propionic Acid ◽

Utilization Efficiency ◽

Tibet Plateau ◽

In Vitro Digestibility ◽

Total Mixed Ration ◽

Aerobic Stability ◽

Fermentation Quality

Objective: To investigate the improvement in utilization efficiency of total mixed ration (TMR) on Tibetan plateau, TMR were ensiled with different additives.Methods: A total of 150 experimental silos were prepared in a completely randomized design to evaluate the six treatments: i) control (without additive), ii) Lactobacillus buchneri (L. buchneri), iii) acetic acid, iv) propionic acid, v) 1,2-propanediol; and vi) 1-propanol. After 90 days of ensiling, silos were opened for fermentation quality and in vitro analysis, and then subjected to an aerobic stability test for 14 days.Results: Treating with L. buchneri, acetic acid, 1,2-propanediol and 1-propanol decreased propionic acid contents and yeast number, whereas increased (p<0.05) pH, acetic acid and ethanol contents in the fermented TMR. Despite increased dry matter (DM) loss in the TMRs treated with 1,2-propanediol and 1-pronanol, additives did not affect (p>0.05) all in vitro parameters including gas production at 24 h (GP24), GP rate constant, potential GP, in vitro DM digestibility and in vitro neutral detergent fibre digestibility. All additives improved the aerobic stability of ensiled TMR to different extents. Specially, aerobic stability of the ensiled TMR were substantially improved by L. buchneri, acetic acid, 1,2-propanediol, and 1-propanol, indicated by stable pH and lactic acid content during the aerobic stability test.Conclusion: L. buchneri, acetic acid, 1,2-propanediol, and 1-propanol had no adverse effect on in vitro digestibility, while ensiling TMR with the additives produced more acetic acid and ethanol, subsequently resulting in improvement of aerobic stability. There is a potential for some fermentation boosting additives to enhance aerobic stability of fermented TMR on Tibetan plateau.

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Propiyonik Asit Katkısının Toplam Karışım Rasyonu Yemin Aerobik Stabilite Özellikleri Üzerine Etkileri

Turkish Journal of Agriculture - Food Science and Technology ◽

10.24925/turjaf.v9i1.210-216.3848 ◽

2021 ◽

Vol 9 (1) ◽

pp. 210-216

Author(s):

Ahmet Aslım ◽

Berrin Okuyucu ◽

Fisun Koç

Keyword(s):

Lactic Acid ◽

Propionic Acid ◽

Storage Conditions ◽

Water Soluble ◽

Neutral Detergent Fiber ◽

Soluble Carbohydrate ◽

Total Mixed Ration ◽

Aerobic Stability ◽

Different Levels ◽

Feed Samples

This study investigated the effects of different levels of propionic acid addition on the aerobic stability characteristics of the total mixture ration. In the study, the effects of four different levels of propionic acid-based additives (0, 1.5, 3.0, 4.5%) on storage conditions of 26°C and 30°C. Feed samples were stored for 7 days, 3 replicates for each treatment group. Chemical and microbiological parameters were analysed in feed samples during aerobic stability. Temperature values and ambient temperature in each treatment were measured and recorded through temperature sensors for 7 days. The addition of additives in the study decreased the pH, dry matter, neutral detergent fiber, and yeast contents of total mixed ration, increased crude protein, eter extract, water soluble carbohydrate, lactic acid, and lactic acid bacteria contents and prevented mold growth. As a result of the research, the addition of 4.5% propionic acid allowed the total mixed ration to remain stable at 26°C for 7 days and 30°C for 5 days.

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