scholarly journals ERYTHROCYTE FREEZING DURING THE MODIFICATION IN COMPOSITION OF CRYOCONSERVANT BASED ON GLYCEROL

2020 ◽  
Vol 20 (2) ◽  
pp. 161-164
Author(s):  
V.V. Ramazanov ◽  
S.V. Rudenko ◽  
A.Yu. Semenchenko ◽  
V.A. Bondarenko
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Osmotic Shock ◽  
Glycerol Concentration ◽  
Internal Organs ◽  
Erythrocyte Hemolysis ◽  
Nacl Concentration ◽  
Standard Composition ◽  
Species Production ◽  
Protective Effectiveness

Damage to red blood cells occurring during freezing can also develop with transfusion. An increase in the concentration of free iron in the blood during the destruction of red blood cells results in an acceleration of the reactive oxygen species production, peroxide damage to cells and stimulation of inflammation. With multiple transfusions of red blood cells, the function of internal organs may be impaired as a result of iron overload. The cryoprotective properties of glycerol include its collegiate ability to reduce the concentration of NaCl at temperatures below 0°C. However, it was found out that the cryoprotective efficacy of glycerol was far less than it was expected from its colligative properties. Damage to red blood cells during their rapid thawing is the result of osmotic shock due to the inability of an excess of intracellular glycerol to exit the cells quickly enough to prevent their swelling and hemolysis. These data indicate the need to reduce the glycerol concentration in the cryoconservant. However, maintaining the protective effectiveness of the cryoprotectant requires a decrease in the NaCl concentration. This study investigates the effect of the composition of the cryoconservant on erythrocyte hemolysis during freezing and hypothermic storage. We used 2 types of cryoconservants: a) 38% (w / v) glycerin, 2.9% sorbitol, 0.63% NaCl (standard composition); b) 28% (w / v) glycerol, 6.8% sorbitol, 0.25% NaCl (modified composition). It was found out that a decrease in the concentration of glycerol and an increase in the concentration of sorbitol leads to a decrease in the erythrocyte hemolysis. The results obtained suggest that a change in the composition of the cryoconservant will reduce osmotic stress during thawing of red blood cells, which, in turn, will reduce their destruction upon transfusion and the likelihood of inflammation.

Membrane Fusion and Deformation of Red Blood Cells by Electric Fields

1980 ◽  
Vol 35 (11-12) ◽  
pp. 1081-1085 ◽  
Author(s):  
Peter Scheurich ◽  
Ulrich Zimmermann ◽  
Maja Mischel ◽  
Ingolf Lamprecht
Keyword(s):  
Electric Field ◽  
Red Blood Cells ◽  
Membrane Fusion ◽  
Electric Fields ◽  
Blood Cells ◽  
Electrical Breakdown ◽  
Osmotic Shock ◽  
Sine Wave ◽  
Hypotonic Solution ◽  
Alternating Electric Field

Abstract Human red blood cells suspended in a slightly hypotonic solution of low electric conductivity were exposed to an inhomogeneous and alternating electric field (sine wave, 30 V peak-to-peak value, electrode distance 120 μm, 0.5 to 2 MHz). Due to the dielectrophoretic effect the cells align parallel to the field lines under the formation of pearl chains. At high voltages (10 V amplitude) membrane fusion is observed between the adhered red blood cells in the pearl chains, whereby the chains become attached to the electrodes. In contrast to the pearl chains observed at voltages of up to 5 V amplitude the resulting fused and uniform aggregates which exhibit no recognisable individual cells under the light microscope, remain stable, even after the alternating electric field has been switched off or after haemolysis in response to osmotic shock. The fused aggregates are highly elastic. If the field strength of the applied alternating electric field is further increased they are stretched in the direction of the opposite electrode. Frequently, bridges are formed between the two electrodes. The uniform bridges remain stable for some time even in the absence of an electric field. The possibility of cell fusion and its initiation by electrical breakdown of the cell membranes are discussed.

Hemoglobin crystals of the red blood cells in the human renal cortex: electron microscopic observations of the renal lithiasis

1978 ◽  
Vol 36 (2) ◽  
pp. 480-481
Author(s):  
Kosuke Ueda ◽  
Hiroto Washida ◽  
Nakazo Watari
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Renal Cortex ◽  
Uranyl Acetate ◽  
Osmic Acid ◽  
Renal Lithiasis ◽  
Electron Microscopic ◽  
Hemoglobin C ◽  
First Case ◽  
Ultrathin Sections

IntroductionHemoglobin crystals in the red blood cells were electronmicroscopically reported by Fawcett in the cat myocardium. In the human, Lessin revealed crystal-containing cells in the periphral blood of hemoglobin C disease patients. We found the hemoglobin crystals and its agglutination in the erythrocytes in the renal cortex of the human renal lithiasis, and these patients had no hematological abnormalities or other diseases out of the renal lithiasis. Hemoglobin crystals in the human erythrocytes were confirmed to be the first case in the kidney.Material and MethodsTen cases of the human renal biopsies were performed on the operations of the seven pyelolithotomies and three ureterolithotomies. The each specimens were primarily fixed in cacodylate buffered 3. 0% glutaraldehyde and post fixed in osmic acid, dehydrated in graded concentrations of ethanol, and then embedded in Epon 812. Ultrathin sections, cut on LKB microtome, were doubly stained with uranyl acetate and lead citrate.

Densitometric Identification of Primitive Erythroblasts After Reaction With Diaminobenzidine

1973 ◽  
Vol 31 ◽  
pp. 328-329
Author(s):  
John A. Trotter
Keyword(s):  
Red Blood Cells ◽  
Analytical Method ◽  
Blood Cells ◽  
Guinea Pigs ◽  
Specific Protein ◽  
Visual Examination ◽  
Hemoglobin Synthesis ◽  
Peroxidatic Activity ◽  
Small Density

Hemoglobin is the specific protein of red blood cells. Those cells in which hemoglobin synthesis is initiated are the earliest cells that can presently be considered to be committed to erythropoiesis. In order to identify such early cells electron microscopically, we have made use of the peroxidatic activity of hemoglobin by reacting the marrow of erythropoietically stimulated guinea pigs with diaminobenzidine (DAB). The reaction product appeared as a diffuse and amorphous electron opacity throughout the cytoplasm of reactive cells. The detection of small density increases of such a diffuse nature required an analytical method more sensitive and reliable than the visual examination of micrographs. A procedure was therefore devised for the evaluation of micrographs (negatives) with a densitometer (Weston Photographic Analyzer).

Scanning electron microscopy of erythrophagocytosis by Entamoeba histolytica trophozoites

1992 ◽  
Vol 50 (1) ◽  
pp. 880-881
Author(s):  
Victor Tsutsumi ◽  
Adolfo Martinez-Palomo ◽  
Kyuichi Tanikawa
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Red Blood Cells ◽  
Entamoeba Histolytica ◽  
Causative Agent ◽  
Blood Cells ◽  
Protozoan Parasite ◽  
Complex Phenomenon ◽  
Great Capacity ◽  
Scanning Electron

The protozoan parasite Entamoeba histolytica is the causative agent of amebiasis in man. The trophozoite or motile form is a highly dynamic and pleomorphic cell with a great capacity to destroy tissues. Moreover, the parasite has the singular ability to phagocytize a variety of different live or death cells. Phagocytosis of red blood cells by E. histolytica trophozoites is a complex phenomenon related with amebic pathogenicity and nutrition.

Ultrastructural observations on the in vitro cytoadherence of Plasmodium falciparum infected red blood cells

1990 ◽  
Vol 48 (3) ◽  
pp. 914-915
Author(s):  
D.J.P. Ferguson ◽  
A.R. Berendt ◽  
J. Tansey ◽  
K. Marsh ◽  
C.I. Newbold
Keyword(s):  
Plasmodium Falciparum ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Umbilical Vein ◽  
Cell Types ◽  
Amelanotic Melanoma ◽  
Cytoplasmic Process ◽  
Umbilical Vein Endothelial Cells

In human malaria, the most serious clinical manifestation is cerebral malaria (CM) due to infection with Plasmodium falciparum. The pathology of CM is thought to relate to the fact that red blood cells containing mature forms of the parasite (PRBC) cytoadhere or sequester to post capillary venules of various tissues including the brain. This in vivo phenomenon has been studied in vitro by examining the cytoadherence of PRBCs to various cell types and purified proteins. To date, three Ijiost receptor molecules have been identified; CD36, ICAM-1 and thrombospondin. The specific changes in the PRBC membrane which mediate cytoadherence are less well understood, but they include the sub-membranous deposition of electron-dense material resulting in surface deformations called knobs. Knobs were thought to be essential for cytoadherence, lput recent work has shown that certain knob-negative (K-) lines can cytoadhere. In the present study, we have used electron microscopy to re-examine the interactions between K+ PRBCs and both C32 amelanotic melanoma cells and human umbilical vein endothelial cells (HUVEC).We confirm previous data demonstrating that C32 cells possess numerous microvilli which adhere to the PRBC, mainly via the knobs (Fig. 1). In contrast, the HUVEC were relatively smooth and the PRBCs appeared partially flattened onto the cell surface (Fig. 2). Furthermore, many of the PRBCs exhibited an invagination of the limiting membrane in the attachment zone, often containing a cytoplasmic process from the endothelial cell (Fig. 2).

Phagocytosis of opsonized red blood cells by hepatocytes after gene transfer of chimeric Fc γR III Aα and γcDNA

2001 ◽  
Vol 120 (5) ◽  
pp. A356-A357
Author(s):  
M FURUKAWA ◽  
Y MAGAMI ◽  
D NAKAYAMA ◽  
F MORIYASU ◽  
J PARK ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Red Blood Cells ◽  
Blood Cells
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