Use of supernatant refractive index and supernatant hemoglobin concentration to assess residual glycerol concentration in cryopreserved red blood cells

2009 ◽  
Vol 408 (1-2) ◽  
pp. 83-86 ◽  
Author(s):  
Kenneth A. Wong ◽  
Nada Nsier ◽  
Jason P. Acker
Keyword(s):  
Refractive Index ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Hemoglobin Concentration ◽  
Glycerol Concentration

scholarly journals Hemoglobin Oxidation in Stored Blood Accelerates Hemolysis and Oxidative Injury to Red Blood Cells

2020 ◽  
Vol 12 (04) ◽  
pp. 244-249
Author(s):  
Ibrahim Mustafa ◽  
Tameem Ali Qaid Hadwan
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Morphological Changes ◽  
Hemoglobin Concentration ◽  
Red Cell Membrane ◽  
Rbc Membrane ◽  
Hemoglobin Oxidation ◽  
Rbc Indices ◽  
Methemoglobin Formation ◽  
Stored Blood

Abstract Introduction Maintaining blood supply is a challenge in blood banks. Red blood cells (RBCs) stored at 4°C experience issues of biochemical changes due to metabolism of cells, leading to changes collectively referred to as “storage lesions.” Oxidation of the red cell membrane, leading to lysis, contributes to these storage lesions. Methods Blood bags with CPD-SAGM stored at 4°C for 28 days were withdrawn aseptically on days 1, 14, and 28. Hematology analyzer was used to investigate RBC indices. Hemoglobin oxidation was studied through spectrophotometric scan of spectral change. RBC lysis was studied with the help of Drabkin's assay, and morphological changes were observed by light and scan electron microscopy. Results RBCs show progressive changes in morphology echinocytes and spherocytes on day 28. There was 0.85% RBC lysis, an approximately 20% decrease in percentage oxyhemoglobin, and a 14% increase in methemoglobin formation, which shows hemoglobin oxidation on day 28. Conclusions Oxidative damage to RBC, with an increase in storage time was observed in the present study. The observed morphological changes to RBC during the course of increased time shows that there is progressive damage to RBC membrane and a decrease in hemoglobin concentration; percentage RBC lysis is probably due to free hemoglobin and iron.

scholarly journals The Effect of Experimental Escherichia coli Infection on Some Blood Parameters and Histological Changes in Male Rats

2011 ◽  
Vol 35 (2) ◽  
pp. 22-27
Author(s):  
Hayder AL – Zamely
Keyword(s):  
Escherichia Coli ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Histopathological Examination ◽  
Hemoglobin Concentration ◽  
Blood Parameters ◽  
Infected Group ◽  
Male Rats ◽  
Control Group ◽  
Renal Tubules

This study was carried out to investigate the effect of experimental infection by Escherichia coli on some blood parameters and histopathological changes in male rats to reach this objective twenty male rats (Rattus norvigicus) were divided into two groups, housed under same conditions of water, feed, temperature, light and humidity. The first one served as control group, while the second was experimentally infected with Ecoli by administration of 2ml / rat of (1×109) colony forming unit/ml of E-coli. Five days later blood were obtained from animals by cardiac puncture to measure the total and differential white blood cells count hemoglobin concentration packed cells volume red blood cells count, and thrombocytes count. One animal from each group was killed and histopathological examination was done for intestine kidney liver and spleen to detect the effect of infection on these organs. The results revealed a significant increase in total leukocytes count (P<005) in infected group while the differential count showed a significant increase in neutrophils and significant decrease in lymphocytes (P< 0.05) in same group. There were a significant decrease in hemoglobin concentration packed cell volume and non significant decrease in red blood cells count. While thrombocytes count showed a significant decrease in infected group compared with control group (P<0.05). The histopathological examination of infected male rats intestine was demonstrated mild diffuse inflammation which include increased numbers of neutrophile in tissue intraepithelial lymphocytes and goblet cells In kidney renal tubules showed extensive epithelial swelling with decreased lumen space and generalized necrotic changes with interstitial hemorrhage in renal cortex. In liver hepatocytes were swollen with decreased sinusoidal spaces and widely distributed necrotic foci were seen. There is extensive splenic necrosis with infiltration of leukocytes from white pulp to red pulp of spleen.

Simultaneous determination of size and refractive index of red blood cells by light scattering measurements

2006 ◽  
Vol 88 (8) ◽  
pp. 084101 ◽  
Author(s):  
N. Ghosh ◽  
P. Buddhiwant ◽  
A. Uppal ◽  
S. K. Majumder ◽  
H. S. Patel ◽  
...  
Keyword(s):  
Refractive Index ◽  
Light Scattering ◽  
Red Blood Cells ◽  
Simultaneous Determination ◽  
Blood Cells ◽  
Scattering Measurements

scholarly journals STUDI KASUS : PROFIL SEL DARAH MERAH ANJING YANG TERINFEKSI BABESIA SP.

2018 ◽  
Vol 6 (1) ◽  
pp. 12-16
Author(s):  
Yanse Yane Rumlaklak ◽  
Jois Moriani Jacob ◽  
Aven B Oematan
Keyword(s):  
Physical Examination ◽  
Red Blood Cells ◽  
Clinical Examination ◽  
Blood Cells ◽  
Clinical Symptoms ◽  
Hemoglobin Concentration ◽  
Blood Parasites ◽  
Blood Collection ◽  
Laboratory Examination ◽  
Blood Tests

Babesia sp. is a protozoa that infects dogs through tick bite and can cause babesiosis. The purpose of blood tests in case dogs is to find out the profile of the dog's red blood cells with babesiosis. On clinical examination found many ticks on the dog's body. Dogs show clinical symptoms of dehydration and paleness in the mucosa. Blood collection is done through the anterior antibrachii cephalica vein. Blood is taken and collected in EDTA tubes. Then the examination is done by examining blood parasites, examining erythrocyte values, examining hemoglobin concentration and hematocrit values. Examination of blood parasites was done by making reticulocyte preparations, while examining erythrocyte values, hemoglobin concentration and hematocrit values were performed using a Vet Scan® HM 5TM machine. The results of examination of reticulocyte preparations showed the existence of Babesia sp infestations with a presentation of 0.68%. Erythrocyte values, hemoglobin concentration and hematocrit values were 3.52x106 // µL, 6.90 g / dL and 23.81%. Based on history, physical examination, clinical and laboratory examination, it can be concluded that the Beagle dog named Loh has microcytic hypochromatic anemia.

scholarly journals КОМПЛЕКСНІ ПРЕПАРАТИ НА ОСНОВІ НАНОБІОМАТЕРІАЛІВ, ПЕРСПЕКТИВИ ВИКОРИСТАННЯ У РЕПРОДУКЦІЇ ОВЕЦЬ ТА КІЗ

2016 ◽  
Vol 18 (2(66)) ◽  
pp. 162-166
Author(s):  
P.M. Sklyarov ◽  
V.P. Koshevoy
Keyword(s):  
Superoxide Dismutase ◽  
Vitamin A ◽  
Red Blood Cells ◽  
Total Protein ◽  
Blood Cells ◽  
Serum Vitamin ◽  
Hemoglobin Concentration ◽  
Oxygen Metabolism ◽  
Zinc Carbonate ◽  
Technical Specifications

A complex preparations «Kahadin + OV», «Kaplaestrol + OV», «Karafest + OV» and «Karafand + OV», which have antioxidant, anti-hypoxic and membranoprotective properties thanks to nanobiomaterials.Were tested drug «Karafest + OV Zn», at doses of justification which came out of the daily needs of animals in the components of the drug, which for sheep and goats in the last 7-8 weeks of pregnancy (per animal), carotene – 0.28–0.5 mg/kg, or 12–26 mg, phytoestrogens – 0,0175 mg/kg or 0.61-1.22 mg, zinc – 1-1.08 mg/kg, or 54-70 mg.The content of substances in 1 ml «Karafest + OV Zn» according to the technical specifications are: carotene - 10 mg (1,2-2,6 ml), phytoestrogens - 1 mg (0.6-1.2 ml), zinc carbonate – 2 mg, ortovanadatе europium activated gadolinium – 0.00015 mg.The final dosage was determined by the scheme – 0.35–2.1 ml/animal оr 0.01–0.03 ml/kg body weight/day.The drug «Karafest + OV Zn» asked orally with food daily for a month to projected pregnancy (140 days of pregnancy).The positive effect of the drug «Karafest + OV Zn» at the homeostasis parameters in sheep, including normalization of serum vitamin A, zinc and total protein; prooxidative-antioxidant system containing serum – malondialdehyde, catalase, superoxide dismutase and erythrocyte – malondialdehyde, catalase, restored glutathione and oxidative-antioxidant value;  system state  oxygen  metabolism  –  the number of red blood cells and hemoglobin concentration of 2.3-dyphosphoglyceratis.Thus, the use of the drug «Karafest + OV Zn» increased content in blood serum, vitamin A (by 16.2%), zinc (by 23,4%), total protein (by 11.5%), malondialdehyde (by 17.5%), catalase (by 24.7%), superoxide dismutase (by 17.9%); in erythrocytes: (by 24.4%), catalase (by 23.9 %) and reduced glutathione (by 18.2%) and hemoglobin (by 23.5 %), the actual number of red blood cells (by 21.8%), the concentration of 2,3-dyphosphoglyceratis (by 22.2%) and normalized prooxidant-antioxidant ratio.

scholarly journals Refractive index of human red blood cells between 290 nm and 1100 nm determined by optical extinction measurements

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Jonas Gienger ◽  
Kathrin Smuda ◽  
Ralph Müller ◽  
Markus Bär ◽  
Jörg Neukammer
Keyword(s):  
Refractive Index ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Optical Extinction ◽  
Human Red Blood Cells

Acellular hemoglobin solution enters compressed lung capillaries more readily than red blood cells

2000 ◽  
Vol 89 (3) ◽  
pp. 1198-1204 ◽  
Author(s):  
Robert L. Conhaim ◽  
Lance A. Rodenkirch ◽  
Kal E. Watson ◽  
Bruce A. Harms
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Hemoglobin Concentration ◽  
Red Cells ◽  
Red Cell ◽  
Hemoglobin Solution ◽  
Lung Inflation ◽  
Hemoglobin Mass ◽  
Lung Capillaries ◽  
Alveolar Septa

High lung inflation pressures compress alveolar septal capillaries, impede red cell transit, and interfere with oxygenation. However, recently introduced acellular hemoglobin solutions may enter compressed lung capillaries more easily than red blood cells. To test this hypothesis, we perfused isolated rat lungs with fluorescently labeled diaspirin cross-linked hemoglobin (DCLHb; 10%) and/ or autologous red cells (hematocrit, 20). Septal capillaries were compressed by setting lung inflation pressure above vascular pressures (zone 1). Examination by confocal microscopy showed that DCLHb was distributed throughout alveolar septa. Furthermore, this distribution was not affected by adding red blood cells to the perfusate. We estimated the maximum acellular hemoglobin mass within septa to be equivalent to that of 15 red blood cells. By comparison, we found an average of 2.7 ± 4.6 red cells per septum in zone 1. These values increased to 30.4 ± 25.8 and 50.4 ± 22.1 cells per septum in zones 2 and 3, respectively. We conclude that perfusion in zone 1 with a 10% acellular hemoglobin solution may increase the hemoglobin concentration per septum up to fivefold compared with red cell perfusion.

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