Inhibitory and eradication activities of 1-monolaurin as anti-biofilm on monospecies and polymicrobial of Staphylococcus epidermidis and Candida tropicalis

2020 ◽  
Vol 13 (01) ◽  
Keyword(s):  
Candida Tropicalis ◽  
Staphylococcus Epidermidis

scholarly journals Pseudoaneurisma micótico por embolismo séptico en paciente pediátrico con leucemia linfoblástica aguda

2019 ◽  
Vol 23 (4) ◽  
pp. 158-162
Author(s):  
Amalia Ojeda ◽  
Juliana Jiménez ◽  
Adalberto Gómez ◽  
Rubén Montoya ◽  
Roy Sanguino
Keyword(s):  
Candida Tropicalis ◽  
Staphylococcus Epidermidis ◽  
Salmonella Spp ◽  
Staphylococcus Spp

Los aneurismas pediátricos son raros y pueden se causados por infección al dañar la pared arterial formando una saculación ciega contigua a su lumen denominada pseudoaneurisma micótico. La mayoría de los casos reportados son de pacientes ancianos con comorbilidades. Los agentes causantes más frecuentes son el Staphylococcus spp, Salmonella spp, Streptococcus sppy raramente hongos. Presentamos el caso de un niño de 3 años, con diagnóstico reciente de leucemia linfoblástica aguda de precursores B en remisión, con riesgo alto de recaída por tratamiento incompleto y antecedente de bacteremia por Staphylococcus epidermidis y fungemia por Cándida tropicalis; con vegetaciones cardiacas que hacen embolismo a hígado, bazo, pulmón y cerebro, en quien se encuentra pseudoaneurisma micótico parcialmente trombosado de la arteria ilíaca común y externa. El diagnóstico temprano de ésta entidad es de vital importancia por el riesgo de ruptura. El manejo quirúrgico dependerá de la localización, tamaño y complicaciones asociadas.

Efficacy of carboxymethyl chitosan against Candida tropicalis and Staphylococcus epidermidis monomicrobial and polymicrobial biofilms

2018 ◽  
Vol 110 ◽  
pp. 150-156 ◽  
Author(s):  
Yulong Tan ◽  
Matthias Leonhard ◽  
Su Ma ◽  
Doris Moser ◽  
Berit Schneider-Stickler
Keyword(s):  
Candida Tropicalis ◽  
Staphylococcus Epidermidis ◽  
Carboxymethyl Chitosan

scholarly journals Presence of Candida tropicalis on Staphylococcus epidermidis Biofilms Facilitated Biofilm Production and Candida Dissemination: An Impact of Fungi on Bacterial Biofilms

2021 ◽  
Vol 11 ◽  
Author(s):  
Pornpimol Phuengmaung ◽  
Wimonrat Panpetch ◽  
Uthaibhorn Singkham-In ◽  
Tanittha Chatsuwan ◽  
Chintana Chirathaworn ◽  
...  
Keyword(s):  
Candida Tropicalis ◽  
Staphylococcus Epidermidis ◽  
Culture Method ◽  
Patient Characteristics ◽  
Sepsis Severity ◽  
Microbial Biofilms ◽  
Biofilm Production ◽  
Single Organism ◽  
Tnf Α ◽  
Bacteria And Fungi

While Staphylococcus epidermidis (SE) is a common cause of infections in implanted prostheses and other indwelling devices, partly due to the biofilm formation, Candida tropicalis (CT) is an emerging Candida spp. with a potent biofilm-producing property. Due to the possible coexistence between SE and CT infection in the same patient, characteristics of the polymicrobial biofilms from both organisms might be different from those of the biofilms of each organism. Then, the exploration on biofilms, from SE with or without CT, and an evaluation on l-cysteine (an antibiofilm against both bacteria and fungi) were performed. As such, Candida incubation in preformed SE biofilms (SE > CT) produced higher biofilms than the single- (SE or CT) or mixed-organism (SE + CT) biofilms as determined by crystal violet staining and fluorescent confocal images with z-stack thickness analysis. In parallel, SE > CT biofilms demonstrated higher expression of icaB and icaC than other groups at 20 and 24 h of incubation, suggesting an enhanced matrix polymerization and transportation, respectively. Although organism burdens (culture method) from single-microbial biofilms (SE or CT) were higher than multi-organism biofilms (SE + CT and SE > CT), macrophage cytokine responses (TNF-α and IL-6) against SE > CT biofilms were higher than those in other groups in parallel to the profound biofilms in SE > CT. Additionally, sepsis severity in mice with subcutaneously implanted SE > CT catheters was more severe than in other groups as indicated by mortality rate, fungemia, serum cytokines (TNF-α and IL-6), and kidney and liver injury. Although CT grows upon preformed SE-biofilm production, the biofilm structures interfered during CT morphogenesis leading to the frailty of biofilm structure and resulting in the prominent candidemia. However, l-cysteine incubation together with the organisms in catheters reduced biofilms, microbial burdens, macrophage responses, and sepsis severity. In conclusion, SE > CT biofilms prominently induced biofilm matrix, fungemia, macrophage responses, and sepsis severity, whereas the microbial burdens were lower than in the single-organism biofilms. All biofilms were attenuated by l-cysteine.

Ensaio farmacológico da Luffa operculata

2014 ◽  
Vol 3 (2) ◽  
pp. 21
Author(s):  
Rebeka Alves Caribé ◽  
Karen Pena de Souza Cavalcanti ◽  
Ivone Antonia de Souza ◽  
Elisangela Christhianne Barbosa da Silva ◽  
Evandro Valentim da Silva ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Candida Tropicalis ◽  
Mus Musculus ◽  
Staphylococcus Epidermidis ◽  
Rio De Janeiro ◽  
Minas Gerais ◽  
Sarcoma 180

O uso de plantas no tratamento e na cura de enfermidades e tão antigo quanto à espécie humana. Luffa operculata cong é nativa da America do Sul especialmente do Brasil, podendo ser encontrada amplamente distribuída em Pernambuco, Bahia, Minas Gerais, Rio de Janeiro e Ceará. Trata-se de uma planta angiosperma e dicotiledônia da família cucurbitaceae, popularmente conhecida como buchinha, cabacinha, purga-de-jalapa e purga-dos-paulistas. Sendo empregada na medicina popular tradicional principalmente para sinusite, rinite e como descongestionante nasal. O trabalho teve como objetivo avaliar a toxicidade aguda, atividade farmacológica (antitumoral em roedores) e microbiológica do extrato bruto etanólico de Luffa operculata cong. Foram realizados ensaios de toxicidade aguda por via intraperitoneal com observações comportamentais para efeitos depressores do Sistema Nervoso Central. A DL50 determinada por via intraperitoneal foi 3,3 mg/kg considerada muito tóxica. Na avaliação antitumoral de Luffa operculata cong frente ao sarcoma 180, células tumorais foram implantadas em camundongos Swiss (Mus musculus) fêmeas sadias. Os animais divididos em grupos de seis, foram tratados com solução fisiológica 0,9% metotrexano (10 mg/kg) e extrato bruto etanólico de Luffa operculata cong nas doses de 0,3; 0,6 e 1,0 mg/kg. Os animais tratados com a maior dose do extrato obtiveram índice de inibição significativo de 61,7% quando comparado com grupo controle. Para os animais tratados com metotrexato (10 mg/kg) a inibição foi de 95,4 %. Na análise microbiológica foram realizadas avaliação da atividade antimicrobiana da Luffa Operculata Cong contra espécies bacterianas e fúngicas da origem clínica da coleção. Os ensaios foram realizados, através do método de difusão em meio sólido, incubados em temperatura de 35 °C durante 7 – 14 dias para espécies fúngicas. Os resultados demonstram que o extrato da Luffa operculata cong apresenta atividade antimicrobiana contra Staphylococcus epidermidis, Candida albicans, e Cândida Tropicalis. Embora seja considerada muito tóxica a Luffa operculata apresentou inibição tumoral em neoplasias malignas em tumor de tecido conjuntivo com perspectivas para novas investigações em outros processos neoplásicos.

Scanning Electron Microscopy of Staphylococcus Epidermidis Adherence to Continuous Ambulatory Peritoneal Dialysis Catheters

1985 ◽  
Vol 43 ◽  
pp. 520-521
Author(s):  
William J. Lamoreaux ◽  
David L. Smalley ◽  
Larry M. Baddour ◽  
Alfred P. Kraus
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Peritoneal Dialysis ◽  
Continuous Ambulatory Peritoneal Dialysis ◽  
Staphylococcus Epidermidis ◽  
Sterile Saline ◽  
Intravascular Devices ◽  
Capd Patient ◽  
Scanning Electron

Infections associated with the use of intravascular devices have been documented and have been reported to be related to duration of catheter usage. Recently, Eaton et al. reported that Staphylococcus epidermidis may attach to silastic catheters used in continuous ambulatory peritoneal dialysis (CAPD) treatment. The following study presents findings using scanning electron microscopy (SEM) of S. epidermidis adherence to silastic catheters in an in vitro model. In addition, sections of polyvinyl chloride (PVC) dialysis bags were also evaluated by SEM.The S. epidermidis strain RP62A which had been obtained in a previous outbreak of coagulase-negative staphylococcal sepsis at local hospitals was used in these experiments. The strain produced surface slime on exposure to glucose, whereas a nonadherent variant RP62A-NA, which was also used in these studies, failed to produce slime. Strains were grown overnight on blood agar plates at 37°C, harvested from the surface and resuspended in sterile saline (0.85%), centrifuged (3,000 rpm for 10 minutes) and then washed twice in 0.1 M phosphate-buffered saline at pH 7.0. Organisms were resuspended at a concentration of ca. 106 CFU/ml in: a) sterile unused dianeal at 4.25% dextrose, b) sterile unused dianeal at 1.5% dextrose, c) sterile used dialysate previously containing 4.25% dextrose taken from a CAPD patient, and d) sterile used dialysate previously containing 1.5% dextrose taken from a CAPD patient.

Endocarditis due to Micrococci and Staphylococcus epidermidis

1973 ◽  
Vol 132 (2) ◽  
pp. 216-220 ◽  
Author(s):  
T. F. Keys
Keyword(s):  
Staphylococcus Epidermidis

Staphylococcus epidermidis endocarditis complicating a Starr-Edwards prosthesis. A therapeutic dilemma

1970 ◽  
Vol 126 (6) ◽  
pp. 1014-1018 ◽  
Author(s):  
C. Watanakunakorn
Keyword(s):  
Staphylococcus Epidermidis

Levels of antibody to Staphylococcus epidermidis in patients with acne vulgaris

1965 ◽  
Vol 92 (1) ◽  
pp. 88-90 ◽  
Author(s):  
S. M. Puhvel
Keyword(s):  
Staphylococcus Epidermidis ◽  
Acne Vulgaris

Untersuchungen zur aeroben bakteriellen Besiedlung der Vaginalschleimhaut klinisch gesunder Kätzinnen

2004 ◽  
Vol 32 (02) ◽  
pp. 88-91
Author(s):  
Susanne Kloß ◽  
A. Wehrend ◽  
Astrid König ◽  
H. Bostedt
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Pasteurella Multocida ◽  
E Coli ◽  
Klinische Relevanz ◽  
Staphylococcus Intermedius

Zusammenfassung: Gegenstand und Ziel: Im Gegensatz zur Hündin liegen bei der Katze bisher wenige Studien über die genitale Keimflora geschlechtsgesunder Tiere vor. Ziel der Untersuchung war daher, physiologische Daten über die aerobe Vaginalflora bei dieser Spezies zu gewinnen. Material und Methoden: Für die vorliegende Studie standen 26 gesunde, anöstrische Katzen zur Verfügung, die zu einer Ovariohysterektomie vorgestellt wurden. Nach einer klinischen Untersuchung wurden von allen Probanden unter sterilen Bedingungen Vaginaltupfer entnommen. Ergebnisse: In allen Proben konnte ein Bakterienwachstum mit durchschnittlich zwei verschiedenen Bakterienspezies nachgewiesen werden. Die Gesamtkeimgehalte wurden bei 50% der Vaginaltupferproben als gering-, bei 15% als mittel- und bei 35% als hochgradig beurteilt. Vorherrschend waren Mischkulturen aus zwei bis vier verschiedenen Keimarten. Monokulturen wurden aus 38% der Tupferproben isoliert. Am häufigsten gelang der Nachweis von E. coli variatio haemolytica (E. coli var. haem.) (58%) und Staphylococcus epidermidis (42%). Als weitere Spezies wurden E. coli, α-, β-hämolysierende Streptokokken, anhämolysierende Streptokokken, aerobe Bazillen, Staphylococcus aureus, Staphylococcus intermedius, Pasteurella multocida sowie Klebsiellen isoliert. Auffällig ist die hohe Nachweisrate von E. coli var. haem. mit 35% in Mischkulturen und 23% in Reinkultur. Schlussfolgerungen: Die physiologische Mikroflora der felinen Vaginalschleimhaut differiert deutlich von der der anöstrischen Hündin. Besonders die Dominanz von E. coli var. haem. in 38% der Mischkulturen und 23% der Monokulturen bei der Katze ist hervorzuheben. Klinische Relevanz: Die vorliegenden Ergebnisse geben eine erste Grundlage für die Interpretation mikrobiologischer Befunde feliner Vaginaltupfer.

Exemplar Abstract for Staphylococcus epidermidis (Winslow and Winslow 1908) Evans 1916 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Staphylococcus Epidermidis
Sign in / Sign up

Export Citation Format

Share Document