scholarly journals ANTISENSE OLIGONUCLEOTIDES AND RADIONUCLIDES – PERSPECTIVES

2021 ◽  
pp. 17-24
Author(s):  
Katya Popova ◽  
Maria Mincheva ◽  
Maria Yavahchova ◽  
Nikolay Goutev ◽  
Dimitar Tonev
Keyword(s):  
Nuclear Medicine ◽  
Clinical Application ◽  
Targeted Therapies ◽  
In Vivo Imaging ◽  
Antisense Oligonucleotides ◽  
Mechanisms Of Action ◽  
New Therapies ◽  
Advantages And Disadvantages ◽  
The Past

Over the past decade, antisense technology has successfully established itself as an entirely innovative platform for research and creation of new therapies. Significant advances in the design of antisense oligonucleotides, as well as a deeper understanding of their mechanisms of action, have led to their successful clinical application in many RNA-targeted therapies. In addition, their potential for in vivo imaging by radiolabeling has been identified. Here are discussed the prospects for the use of antisense oligonucleotides in nuclear medicine and highlighted some of the advantages and disadvantages of labelling them with radionuclides.

scholarly journals A blueprint for translational regenerative medicine

2020 ◽  
Vol 12 (572) ◽  
pp. eaaz2253
Author(s):  
James P. K. Armstrong ◽  
Timothy J. Keane ◽  
Anne C. Roques ◽  
P. Stephen Patrick ◽  
Claire M. Mooney ◽  
...  
Keyword(s):  
Immune Response ◽  
Regenerative Medicine ◽  
In Vivo Imaging ◽  
Imaging Modalities ◽  
Proof Of Concept ◽  
The Past ◽  
Valley Of Death ◽  
Clinical Adoption ◽  
Selection Of

The past few decades have produced a large number of proof-of-concept studies in regenerative medicine. However, the route to clinical adoption is fraught with technical and translational obstacles that frequently consign promising academic solutions to the so-called “valley of death.” Here, we present a proposed blueprint for translational regenerative medicine. We offer principles to help guide the selection of cells and materials, present key in vivo imaging modalities, and argue that the host immune response should be considered throughout design and development. Last, we suggest a pathway to navigate the often complex regulatory and manufacturing landscape of translational regenerative medicine.

Carbon-11 Labeled Tracers for In Vivo Imaging of P-Glycoprotein Function: Kinetics, Advantages and Disadvantages

2010 ◽  
Vol 10 (17) ◽  
pp. 1820-1833 ◽  
Author(s):  
Gert Luurtsema ◽  
Joost Verbeek ◽  
Mark Lubberink ◽  
Adriaan A. Lammertsma ◽  
Rudi Dierckx ◽  
...  
Keyword(s):  
In Vivo Imaging ◽  
Advantages And Disadvantages ◽  
P Glycoprotein

Drug discovery in rare indications: opportunities and challenges

Hematology ◽  
2013 ◽  
Vol 2013 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Victoria M. Richon
Keyword(s):  
Drug Discovery ◽  
Rare Diseases ◽  
Targeted Therapies ◽  
Rapid Growth ◽  
Genetic Alterations ◽  
New Drugs ◽  
New Therapies ◽  
The Past ◽  
Select Patient ◽  
Patient Subgroups

Abstract Over the past decade, the number of new therapies developed for the treatment of rare diseases continues to increase. The most rapid growth has been in the development of new drugs for oncology indications. One focus in drug discovery for oncology indications is the development of targeted therapies for select patient subgroups characterized by genetic alterations. The identification of these patient subgroups has increased in the past decade and has resulted in a corresponding increase in the development of new drugs for genetically defined patient subgroups. As an example of the development of new therapeutics for rare indications, I describe here the drug discovery efforts leading to the development of DOT1L inhibitors for the treatment of MLL-rearranged leukemia.

scholarly journals In vivo imaging and tracking of exosomes for theranostics

2021 ◽  
pp. 2130005
Author(s):  
Ning Ma ◽  
Changfeng Wu ◽  
Zihui Meng
Keyword(s):  
Clinical Application ◽  
In Vivo Imaging ◽  
Photoacoustic Imaging ◽  
Imaging Techniques ◽  
Cell Communication ◽  
Nuclear Imaging ◽  
Future Research ◽  
Uptake Mechanism ◽  
Lipid Bilayer Vesicles

Exosomes are lipid bilayer vesicles released by cells and serve as natural carriers for cell–cell communication. Exosomes provide a promising approach to the diagnosis and treatment of diseases and are considered as an alternative to cell therapy. However, one main restriction in their clinical application is that the current understanding of these vesicles, especially their in vivo behaviors and distributions, remains inadequate. Here, we reviewed the current and emerging methods for in vivo imaging and tracking of exosomes, including fluorescence imaging, bioluminescence imaging, nuclear imaging, X-ray imaging, magnetic resonance imaging, photoacoustic imaging, and multimodal imaging. In vivo imaging and tracking of exosomes by these methods can help researchers further understand their uptake mechanism, biodistribution, migration, function, and therapeutic performance. The pioneering studies in this field can elucidate many unknown exosomal behaviors at different levels. We discussed the advantages and limitations of each labeling and imaging strategy. The advances in labeling and in vivo imaging will expand our understanding of exosomes and promote their clinical application. We finally provide a perspective and discuss several important issues that need to be explored in future research. This review highlights the values of efficient, sensitive, and biocompatible exosome labeling and imaging techniques in disease theranostics.

In Vivo Genetic Strategies for the Specific Lineage Tracing of Stem Cells

2019 ◽  
Vol 14 (3) ◽  
pp. 230-238
Author(s):  
Hong Fan ◽  
Xinyu Liu ◽  
Yahui Shen ◽  
Siwei Chen ◽  
Yu Huan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell Niche ◽  
Lineage Tracing ◽  
Genetic Tools ◽  
Advantages And Disadvantages ◽  
The Past ◽  
Cell Niche ◽  
Genetic Strategies

Background:Characterization of the fate changes of stem cells is essential to understand the roles of certain stem cells both during development and in diseases, such as cancer. In the past two decades, more and more importance has been paid to the studies of in vivo lineage tracing, because they could authentically reveal the differentiation, migration and even proliferation of stem cells. However, specific genetic tools have only been developed until recently.Objective:To summarize the progresses of genetic tools for specific lineage tracing with emphasis on their applications in investigating the stem cell niche signals.Results:Three major genetic strategies have been reviewed according to the development of technique, particularly the advantages and disadvantages of individual methods.Conclusion:In vivo specific lineage tracing of stem cells could be achieved by comprehensive application of multiple genetic tools.

Restraining small decapods and amphipods for in vivo laboratory studies

Crustaceana ◽  
2018 ◽  
Vol 91 (5) ◽  
pp. 517-525 ◽  
Author(s):  
Anton Gurkov ◽  
Ekaterina Borvinskaya ◽  
Ekaterina Shchapova ◽  
Maxim Timofeyev
Keyword(s):  
In Vivo Imaging ◽  
Small Animal ◽  
Laboratory Studies ◽  
Advantages And Disadvantages ◽  
Freely Moving ◽  
Physiological Studies

Abstract During physiological studies, it is often required to restrain a small animal for an experiment, as it is typically challenging to perform in vivo imaging or measurements on freely moving individuals. In this article we describe two widely applicable approaches for repeated restraint of small (approx. 0.5-4 cm) decapods and amphipods that are established in our laboratory: immobilization using gentle gluing and suction. Application of both these approaches as well as their advantages and disadvantages are discussed in detail.

scholarly journals Hydrogen peroxide: history of discovery, chemical and biochemical aspects, place of formation and role in the body (review)

2020 ◽  
pp. 25-31
Author(s):  
Д.А. Еникеев ◽  
К.О. Кузнецов ◽  
О.А. Еникеев ◽  
Д.Р. Кузнецова ◽  
Э.Н. Хисамов ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Human Body ◽  
Mechanisms Of Action ◽  
The Body ◽  
Review Of Literature ◽  
Animal Cells ◽  
The Past ◽  
History Of

В статье приведен обзор литературы за последние 100 лет. Затронута история открытия и применения перекиси водорода в различные годы. Подробно описаны химические и физические свойства перекиси водорода, её механизмы действия in vivo и in vitro. Затронута тема образования перекиси водорода в собственных клетках организма человека и животных, описаны физиологические функции эндогенной перекиси водорода в человеческом теле. The article provides a review of literature for the past 100 years; touches on the history of discovery and the use of hydrogen peroxide in different years; describes in detail chemical and physical properties of hydrogen peroxide, and its mechanisms of action in vivo and in vitro. The review addresses the formation of hydrogen peroxide in human and animal cells and describes physiological functions of endogenous hydrogen peroxide in the human body.

Osseointegration interface of calcified bone and endosseous implants

1992 ◽  
Vol 50 (2) ◽  
pp. 1096-1097
Author(s):  
K.E. Krizan ◽  
J.E. Laffoon ◽  
M.J. Buckley
Keyword(s):  
Structure And Function ◽  
Titanium Implants ◽  
En Bloc ◽  
Endosseous Implants ◽  
Ultrastructural Studies ◽  
The Past ◽  
Cacodylate Buffer ◽  
One Year ◽  
And Function

With increase use of tissue-integrated prostheses in recent years it is a goal to understand what is happening at the interface between haversion bone and bulk metal. This study uses electron microscopy (EM) techniques to establish parameters for osseointegration (structure and function between bone and nonload-carrying implants) in an animal model. In the past the interface has been evaluated extensively with light microscopy methods. Today researchers are using the EM for ultrastructural studies of the bone tissue and implant responses to an in vivo environment. Under general anesthesia nine adult mongrel dogs received three Brånemark (Nobelpharma) 3.75 × 7 mm titanium implants surgical placed in their left zygomatic arch. After a one year healing period the animals were injected with a routine bone marker (oxytetracycline), euthanized and perfused via aortic cannulation with 3% glutaraldehyde in 0.1M cacodylate buffer pH 7.2. Implants were retrieved en bloc, harvest radiographs made (Fig. 1), and routinely embedded in plastic. Tissue and implants were cut into 300 micron thick wafers, longitudinally to the implant with an Isomet saw and diamond wafering blade [Beuhler] until the center of the implant was reached.

Sign in / Sign up

Export Citation Format

Share Document