scholarly journals Fine mapping and candidate gene identification of a soybean seed protein and oil QTL from a wild soybean accession and linkage analysis for whole plant biomass, carbon, nitrogen, and seed composition using a RIL mapping population

2021 ◽  
Author(s):  
◽  
Yia Yang
Keyword(s):  
Linkage Analysis ◽  
Carbon Content ◽  
Nitrogen Content ◽  
Seed Protein ◽  
Plant Biomass ◽  
Mapping Population ◽  
Wild Soybean ◽  
Gene Identification ◽  
Plant Nitrogen ◽  
Whole Plant

Soybean [Glycine max (L.) Merr] cultivars have low genetic variation due to domestication, founder events, and selection strategies for modern plant breeding. There is a need to introduce genetic diversity into soybean cultivars for long-term improvement of agronomic and seed compositional traits. In both public and private soybean breeding programs, the introgression of wild soybean (Glycine soja Siebold and Zucc.) genes has been utilized to incorporate novel genetic diversity. In our study, 3,015 single F[subscript 4:9] soybean plants were genotyped for nine genotype-by-sequencing markers from a previous genetic mapping study on recombinant inbred lines (La, 2018) to create two residual heterozygotes derived near isogenic lines (RHD-NIL) populations. The first RHD-NIL population was selected for a novel oil quantitative trait loci (QTL) on chromosome 8 and the second RHD-NIL population was selected for a novel protein QTL on chromosome 14. Both novel QTL derived from the wild soybean accession PI 593983. The objective of this research is to validate these QTL, reduce the QTL interval, and fine map the two novel QTL for candidate gene identification. Single marker analysis and linkage analysis was conducted using SoySNP6K BeadChip markers for QTL validation. The chromosome 8 oil QTL was not advanced for fine mapping because the QTL was not validated in a subsequent field and greenhouse study. Whole genome resequencing was leveraged to reduce the QTL from 16.5 Mbp to approximately 4.6 Mbp and to fine map 50 high protein RHD-NIL, which have segregated for the validated chromosome 14 QTL to permit candidate gene identification. A total of 55 potential candidates was identified in a physical interval of 8,059,955 to 12,648,760 bp. Our results provide a better insight of utilizing wild soybean as a source of genetic diversity for soybean cultivar improvement. In addition to the fine mapping and candidate gene identification study, we conducted linkage analysis for a recombinant inbred line (RIL) mapping population for plant biomass content, whole plant carbon content, whole plant nitrogen content, seed oil content, and seed protein content. Soybean seeds require a large amount of nitrogen because of its high protein content. Through a symbiotic association between soil microorganisms and soybean root nodules, soybean is able to fix atmospheric dinitrogen for nitrogen uptake. Plant biomass was collected by bulking five soybean shoot samples per plot from 262 plots in four locations and bulking three soybean shoots samples per plot from 262 plots in one location. Plant materials were dried and weighed for whole plant biomass weight. Whole plant carbon content, whole plant nitrogen content, seed oil content, and seed protein content was analyzed via near infrared spectroscopy. The objective of this study was to examine nitrogen mobilization from a mapping population from the cross PI 361103 (contains high shoot N content and low seed N content) x PI 567572B (contains high seed N content and low shoot N content), identify QTL for plant biomass, whole plant carbon content, whole plant nitrogen content, and seed composition, and study maternal effects of cytoplasmic inheritance of the five traits from the reciprocal parental cross. Linkage analysis was conducted using BARCSoySNP50K markers. We identified six QTL for plant biomass, two QTL for whole plant carbon content, three QTL for whole plant nitrogen content, three QTL for seed oil content, and five QTL for seed protein content, with multiple traits having overlapping QTL intervals. Our results indicate QTL associated with multiple traits demonstrating the potential of pleiotropic effect in our mapping population.

Rhizobial inoculant formulations and soil pH influence field pea nodulation and nitrogen fixation

2000 ◽  
Vol 80 (3) ◽  
pp. 395-400 ◽  
Author(s):  
W. A. Rice ◽  
G. W. Clayton ◽  
P. E. Olsen ◽  
N. Z. Lupwayi
Keyword(s):  
Nitrogen Content ◽  
Soil Ph ◽  
Crop Production ◽  
Plant Biomass ◽  
Acid Soil ◽  
Dry Weight ◽  
Field Pea ◽  
Total Biomass ◽  
Plant Nitrogen ◽  
Liquid Inoculant

Crop production systems that include field pea (Pisum sativum L.) in rotation are important for sustainable agriculture on acid soils in northwestern Canada. Greenhouse experiments were conducted to compare the ability of liquid inoculant applied to the seed, powdered peat inoculant applied to the seed, and granular inoculant applied in a band with the seed to establish effective nodulation on field pea grown at soil pH(H2O) 4.4, 5.4 and 6.6. Plants were grown to the flat pod stage, and then total plant biomass dry weight, dry weight of nodules, number of nodules, plant nitrogen content, and proportion of plant nitrogen derived from the atmosphere (%Ndfa) were measured. Granular and powdered peat inoculants produced greater nodule numbers and weight, plant nitrogen content, %Ndfa and total biomass than liquid inoculant in at least two of the three experiments. Only granular inoculant was effective in establishing nodules at soil pH 4.4, but granular and powdered peat inoculants were effective at pH 5.4, and all three formulations were effective at pH 6.6. The results showed that granular inoculant has potential for effective nodulation of field pea grown on acid soil. Key words: Rhizobium, inoculant formulations, field pea, nodulation, acid soil

scholarly journals Role of Methylotrophy During Symbiosis Between Methylobacterium nodulans and Crotalaria podocarpa

2005 ◽  
Vol 18 (10) ◽  
pp. 1061-1068 ◽  
Author(s):  
Philippe Jourand ◽  
Adeline Renier ◽  
Sylvie Rapior ◽  
Sergio Miana de Faria ◽  
Yves Prin ◽  
...  
Keyword(s):  
Root Nodule ◽  
Wild Type Strain ◽  
Plant Biomass ◽  
Root Nodules ◽  
Methylotrophic Bacterium ◽  
Plant Nitrogen ◽  
Affected Plant ◽  
Whole Plant ◽  
Fixation Capacity

Some rare leguminous plants of the genus Crotalaria are specifically nodulated by the methylotrophic bacterium Methylobacterium nodulans. In this study, the expression and role of bacterial methylotrophy were investigated during symbiosis between M. nodulans, strain ORS 2060T, and its host legume, Crotalaria podocarpa. Using lacZ fusion to the mxaF gene, we showed that the methylotroph genes are expressed in the root nodules, suggesting methylotrophic activity during symbiosis. In addition, loss of the bacterial methylotrophic function significantly affected plant development. Indeed, inoculation of M. nodulans nonmethylotroph mutants in C. podocarpa decreased the total root nodule number per plant up to 60%, decreased the whole-plant nitrogen fixation capacity up to 42%, and reduced the total dry plant biomass up to 46% compared with the wild-type strain. In contrast, inoculation of the legume C. podocarpa with nonmethylotrophic mutants complemented with functional mxa genes restored the symbiotic wild phenotype. These results demonstrate the key role of methylotrophy during symbiosis between M. nodulans and C. podocarpa.

scholarly journals Development and evaluation of a new Canadian spring wheat sub-model for DNDC

2011 ◽  
Vol 91 (4) ◽  
pp. 503-520 ◽  
Author(s):  
R. Kröbel ◽  
W. Smith ◽  
B. Grant ◽  
R. Desjardins ◽  
C. Campbell ◽  
...  
Keyword(s):  
Nitrogen Content ◽  
Soil Carbon ◽  
Spring Wheat ◽  
Plant Biomass ◽  
Dndc Model ◽  
Plant Nitrogen ◽  
Annual Variations ◽  
Using Data ◽  
Biomass Plant ◽  
Better Than

Kröbel, R., Smith, W. N., Grant, B. B., Desjardins, R. L., Campbell, C. A., Tremblay, N., Li, C. S., Zentner, R. P. and McConkey, B. G. 2011. Development and evaluation of a new Canadian spring wheat sub-model for DNDC. Can. J. Soil Sci. 91: 503–520. In this paper, the ability of the DNDC model (version 93) to predict biomass production, grain yield and plant nitrogen content was assessed using data from experiments at Swift Current, Saskatchewan, and St-Blaise, Quebec, Canada. While predicting wheat grain yields reasonably well, the model overestimated the growth of above-ground plant biomass and nitrogen uptake during the first half of the growing season. A new spring wheat sub-model (DNDC-CSW) was introduced with a modified plant biomass growth curve, dynamic plant C/N ratios and modified plant biomass fractioning curves. DNDC-CSW performed considerably better in simulating plant biomass [modeling efficiency (EF): 0.75, average relative error (ARE): 6.0%] and plant nitrogen content (EF: 0.61, ARE: −2.7%) at Swift Current and St-Blaise (EF of 0.75 and ARE of 2.3%), compared with DNDC 93 (biomass SC: EF 0.49, ARE 17.1%, SB: EF 0.02 ARE 33.4%). In comparison with DNDC 93, DNDC-CSW better captured inter-annual variations in crop growth for a range of wheat rotations, increasing the EF from 0.32 to 0.52 for grain and from 0.35 to 0.39 for straw yields. DNDC-CSW also performed considerably better than DNDC 93 in estimating soil carbon changes at Swift Current. Hence, DNDC-CSW has the potential to improve the performance of DNDC 93 in simulating wheat biomass, plant nitrogen, yield and soil carbon at various Canadian sites.

МАТЕМАТИЧЕСКОЕ МОДЕЛИРОВАНИЕ ДИНАМИКИ МИНЕРАЛЬНОГО ПИТАНИЯ РАСТЕНИЙ В СИСТЕМЕ «УДОБРЕНИЕ-ПОЧВА-РАСТЕНИЕ»

2020 ◽  
Vol 65 (6) ◽  
pp. 1219-1229
Author(s):  
В.А. Четырбоцкий ◽  
◽  
А.Н. Четырбоцкий ◽  
Б.В. Левин ◽  
◽  
...  
Keyword(s):  
Experimental Data ◽  
Temporal Dynamics ◽  
Plant Biomass ◽  
Environmental Parameters ◽  
Parametric Identification ◽  
Agricultural Crop ◽  
Parameter System ◽  
Plant Nitrogen ◽  
Rhizosphere Microorganisms ◽  
Nitrogen Phosphorus

A numerical simulation of the spatial-temporal dynamics of a multi-parameter system is developed. The components of this system are plant biomass, mobile and stationary forms of mineral nutrition elements, rhizosphere microorganisms and environmental parameters (temperature, humidity, acidity). Parametric identification and verification of the adequacy of the model were carried out based on the experimental data on the growth of spring wheat «Krasnoufimskaya-100» on peat lowland soil. The results are represented by temporal distributions of biomass from agricultural crop under study and the findings on the content of main nutrition elements within the plant (nitrogen, phosphorus, potassium). An agronomic assessment and interpretation of the obtained results are given.

scholarly journals Linkage Analysis and QTL Mapping Using SNP Dosage Data in a Tetraploid Potato Mapping Population

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e63939 ◽  
Author(s):  
Christine A. Hackett ◽  
Karen McLean ◽  
Glenn J. Bryan
Keyword(s):  
Linkage Analysis ◽  
Qtl Mapping ◽  
Mapping Population ◽  
Tetraploid Potato

Systemic control of nodule formation by the plant N demand requires autoregulation dependent and independent mechanisms

2021 ◽  
Author(s):  
Marjorie Pervent ◽  
Ilana Lambert ◽  
Marc Tauzin ◽  
Alicia Karouani ◽  
Martha Nigg ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Nodule Formation ◽  
Atmospheric Nitrogen ◽  
Plant Nitrogen ◽  
Systemic Signaling ◽  
Systemic Control ◽  
Whole Plant ◽  
Strong Control ◽  
Mutant Genes ◽  
N Demand

Abstract In legumes interacting with rhizobia the formation of symbiotic organs involved in the acquisition of atmospheric nitrogen is depending of the plant nitrogen (N) demand. We used Medicago truncatula plants cultivated in split-root systems to discriminate between responses to local and systemic N signalings. We evidenced a strong control of nodule formation by systemic N-signaling but obtained no clear evidence of a local control by mineral nitrogen. Systemic signaling of the plant N demand controls numerous transcripts involved in the root transcriptome reprogramming associated to early rhizobia interaction and nodule formation. SUNN has an important role in this control but major systemic N signaling responses remained active in the sunn mutant. Genes involved in the activation of nitrogen fixation are regulated by systemic N signaling in the mutant, explaining why the hypernodulation phenotype is not associated to a higher nitrogen fixation of the whole plant. The control of the transcriptome reprogramming of nodule formation by systemic N signaling requires other pathway(s) that parallel the SUNN/CLE pathway.

scholarly journals Short-Term Decomposition and Nutrient-Supplying Ability of Sewage Sludge Digestate, Digestate Compost, and Vermicompost on Acidic Sandy and Calcareous Loamy Soils

Agronomy ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2249
Author(s):  
Nikolett Uzinger ◽  
Orsolya Szécsy ◽  
Nóra Szűcs-Vásárhelyi ◽  
István Padra ◽  
Dániel Benjámin Sándor ◽  
...  
Keyword(s):  
Organic Matter ◽  
Sewage Sludge ◽  
Organic Carbon ◽  
Carbon Content ◽  
Plant Biomass ◽  
Test Plant ◽  
Organic Carbon Content ◽  
Short Term ◽  
Mineral N ◽  
Average Residual

Organic waste and the compost and vermicompost derived from it may have different agronomic values, but little work is available on this aspect of sewage sludge. A 75-day pot experiment with perennial ryegrass (Lolium perenne) as the test plant aimed to investigate the fertiliser value and organic matter replenishment capacity of digested sewage sludge (DS) and the compost (COM) and vermicompost (VC) made from it, applied in 1% and 3% doses on acidic sand and calcareous loam. The NPK content and availability, changes in organic carbon content and plant biomass, and the efficiency of the amendments as nitrogen fertilisers were investigated. The final average residual carbon content for DS, COM, and VC was 35 ± 34, 85 ± 46, and 55 ± 46%, respectively. The organic carbon mineralisation rate depended on the soil type. The additives induced significant N mineralisation in both soils: the average increment in mineral N content was 1.7 times the total added N on acidic sand and 4.2 times it on calcareous loam for the 1% dose. The agronomic efficiency of COM and VC as fertilisers was lower than that of DS. In the short term, DS proved to be the best fertiliser, while COM was the best for organic matter replenishment.

scholarly journals Different Growth and Sporulation Responses to Temperature Gradient among Obligate Apomictic Strains of Ulva prolifera

Plants ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2256
Author(s):  
Yoichi Sato ◽  
Yutaro Kinoshita ◽  
Miho Mogamiya ◽  
Eri Inomata ◽  
Masakazu Hoshino ◽  
...  
Keyword(s):  
Temperature Gradient ◽  
Carbon Content ◽  
Nitrogen Content ◽  
Environmental Conditions ◽  
Genetic Stability ◽  
Ulva Prolifera ◽  
Market Demand ◽  
Gene Sequences ◽  
Site Specific

The green macroalga Ulva prolifera has a number of variants, some of which are asexual (independent from sexual variants). Although it has been harvested for food, the yield is decreasing. To meet market demand, developing elite cultivars is required. The present study investigated the genetic stability of asexual variants, genotype (hsp90 gene sequences) and phenotype variations across a temperature gradient (10–30 °C) in an apomictic population. Asexual variants were collected from six localities in Japan and were isolated as an unialgal strain. The hsp90 gene sequences of six strains were different and each strain included multiple distinct alleles, suggesting that the strains were diploid and heterozygous. The responses of growth and sporulation versus temperature differed among strains. Differences in thermosensitivity among strains could be interpreted as the result of evolution and processes of adaptation to site-specific environmental conditions. Although carbon content did not differ among strains and cultivation temperatures, nitrogen content tended to increase at higher temperatures and there were differences among strains. A wide variety of asexual variants stably reproducing clonally would be advantageous in selecting elite cultivars for long-term cultivation. Using asexual variants as available resources for elite cultivars provides potential support for increasing the productivity of U. prolifera.

scholarly journals Effects of Heterogeneous Environment After Deforestation on Plant Phenotypic Plasticity of Three Shrubs Based on Leaf Traits and Biomass Allocation

2021 ◽  
Vol 9 ◽  
Author(s):  
Jinniu Wang ◽  
Jing Gao ◽  
Yan Wu ◽  
Bo Xu ◽  
Fusun Shi ◽  
...  
Keyword(s):  
Phenotypic Plasticity ◽  
Carbon Content ◽  
Nitrogen Content ◽  
Leaf Area ◽  
Biomass Allocation ◽  
Principal Components ◽  
Leaf Traits ◽  
Adaptive Plasticity ◽  
Heterogeneous Environments ◽  
Heterogeneous Environment

Phenotypic plasticity among natural plant populations is a species-specific ecological phenomenon of paramount importance that depends on their life forms, development stages, as well as environmental factors. While this phenomenon is broadly understood, it has hardly been observed in nature. This study aimed at understanding phenotypic plasticity and ecological adaptability in three shrubs (Salix etosia, Rubus setchuenensis, and Hydrangea aspera) affected by potential environmental variables after deforesting in sparse Larix spp. forest and tall shrub mixed secondary forests. Soil organic carbon content, total nitrogen content, and available nitrogen content were greater outside the forests, contrary to other measured factors whose availability was higher in the forest interiors. In case of leaf traits and stoichiometric indicators, there were significant interactions of leaf area (LA), leaf dry matter (DW), specific leaf area (SLA), and leaf phosphorus content (LPC) between shrub species and heterogeneous environments (P < 0.05) but not for leaf C/N, N/P, and C/P. Principal components analysis (PCA) indicated that soil temperature, pH value, soil carbon content, soil nitrogen content, and MBC and MBN mainly constituted the first component. Summarized results indicated that TB and leaf C/P of S. etosia were significantly correlated with three principal components, but only marginal significant correlations existed between R/S and relevant components. SLA and R/S of R. setchuenensis had marginal significant relationships with independent variables. Both SLA and TB of H. aspera were significantly correlated with three principal components. Based on the pooled values of leaf functional traits and leaf stoichiometric indicators, R. setchuenensis (vining type) had better leaf traits plasticity to adapt to a heterogeneous environment. In descending order, the ranks of biomass allocation plasticity index of three shrubs were H. aspera (bunch type), R. setchuenensis (vining type), and S. etosia (erect type). The highest integrated plasticity values of leaf traits and biomass allocation was observed in H. aspera (bunch type), followed by R. setchuenensis, and by S. etosia with less adaptive plasticity in heterogeneous environments.

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