scholarly journals Effect of cysteine in maturation medium on embryos development by in vitro fertilization (IVF) in porcine

2016 ◽  
Vol 19 (2) ◽  
pp. 19-26
Author(s):  
Binh Thanh Nguyen
Keyword(s):  
In Vitro Fertilization ◽  
Oocyte Maturation ◽  
Fertilization Rate ◽  
Development Rate ◽  
Maturation Time ◽  
Vitro Fertilization ◽  
Glutathione Concentration ◽  
Maturation Medium

In this article, we studied the effect of cystein in maturation culture on glutathione concentration in oocyte and on embryos development by in vitro fertilization in porcine. 0.6 mM cysteine supplementation on TCM-199 medium has increased the amount of GSH in the oocyte. The GSH level began to increase at the 40th hour after cystein supple metation and attained the highest at the 44th hour (18.32 pmol/oocyte). After 5 days of embryos culture, a total of 1000 oocyte maturation and fertilization was obtained including 583 embryos containing 2 cells, 408 embryos containing 3-4 cells, 264 embryos containing 5-8 cells and 106 morula. In particular, fertilization rate and embryo division was the best in the oocyte maturation of the 44th hour with 200 embryos containing 2 cell (80 %), 161 embryos containing 3-4 cells (64.4 %), 114 embryos containing 5-8 cells (45.6 %) and 62 morula (24.8 %). The results showed that the time of the cysteine supplementation into medium and the time of muturation affected oocyte GSH level. In addition, embryoic development rate was also affected by maturation time and oocyte GSH level.

scholarly journals A Brief Incubation of Cumulus-Enclosed Mouse Eggs in a Calcium-Free Medium Containing a High Concentration of Calcium-Chelator Markedly Improves Preimplantation Development

2020 ◽  
Vol 17 (10) ◽  
pp. 3505
Author(s):  
Valeria Merico ◽  
Silvia Garagna ◽  
Maurizio Zuccotti
Keyword(s):  
In Vitro Fertilization ◽  
Mammalian Species ◽  
Developmental Rate ◽  
Cumulus Cells ◽  
Fertilization Rate ◽  
Preimplantation Development ◽  
High Concentration ◽  
Vitro Fertilization ◽  
Positive Effects

The presence of cumulus cells (CCs) surrounding ovulated eggs is beneficial to in vitro fertilization and preimplantation development outcomes in several mammalian species. In the mouse, this contribution has a negligible effect on the fertilization rate; however, it is not yet clear whether it has positive effects on preimplantation development. Here, we compared the rates of in vitro fertilization and preimplantation development of ovulated B6C3F1 CC-enclosed vs. CC-free eggs, the latter obtained either after a 5 min treatment in M2 medium containing hyaluronidase or after 5–25 min in M2 medium supplemented with 34.2 mM EDTA (M2-EDTA). We found that, although the maintenance of CCs around ovulated eggs does not increment their developmental rate to blastocyst, the quality of the latter is significantly enhanced. Most importantly, for the first time, we describe a further quantitative and qualitative improvement, on preimplantation development, when CC-enclosed eggs are isolated from the oviducts in M2-EDTA and left in this medium for a total of 5 min prior to sperm insemination. Altogether, our results establish an important advancement in mouse IVF procedures that would be now interesting to test on other mammalian species.

scholarly journals Efficiency Comparison Between Dry And Humid Cultures For Clinical Outcome of The In Vitro Fertilization-Embryo Transfer

2021 ◽  
Author(s):  
Weihai Xu ◽  
Lin Zhang ◽  
Ling Zhang ◽  
Shishi Li ◽  
Jing Shu
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Transfer ◽  
Implantation Rate ◽  
Fertilization Rate ◽  
Cleavage Rate ◽  
High Quality ◽  
Vitro Fertilization ◽  
Efficiency Comparison ◽  
Ivf Et

Abstract Background: In this study, we compared the in vitro embryo development, embryo transfer outcome and the offspring outcome in the in vitro fertilization-embryo transfer (IVF-ET) between dry culture (DC) and humid culture (HC). Methods: Our study was divided into two parts. Firstly, we determined the fertilization rate, cleavage rate and high-quality embryo rate from 21 cycles in the DC group (N=262 oocytes) and HC group (N=263 oocytes). Secondly, we determined the embryo transfer outcome and the offspring outcome in DC group (N=184 cycles) and HC group (N=136 cycles). Results: Compared with the HC group, significant increase was observed in the high-quality embryo rate (66.1.2% vs. 55.3%, p=0.037) and implantation rate (49.8% vs. 40.6%, p=0.027) in the DC group. No statistical differences were observed in the pregnant outcome and birth defect of the offspring (p>0.05). Compared with HC, DC was associated with a higher high-quality embryo rate and a higher implantation rate after embryo transfer. Conclusions: No statistical differences were noticed in the offspring conditions between the two culture modes. Taken together, DC may serve as a promising method for IVF-ET.

scholarly journals Preparation of cyclodextrin nanoparticles and evaluation of its effect on the capacitation of bovine spermatozoa used in the in vitro fertilization

2018 ◽  
Vol 9 (4) ◽  
pp. 112
Author(s):  
Heba F Salem ◽  
Mai Raslan ◽  
Hanaa Suliman ◽  
Tamer Essam ◽  
Saber Abd-Allah
Keyword(s):  
Data Analysis ◽  
In Vitro Fertilization ◽  
Interval Data ◽  
Fertilization Rate ◽  
Vitro Fertilization ◽  
Bovine Spermatozoa ◽  
The Media ◽  
Biomedical Interventions ◽  
Positive Effect

<p>This study was conducted to produce nanosized cyclodextrin (NCD) and assess its effect on bovine spermatozoa during In vitro fertilization (IVF) to optimize the capacitation media for successful IVF. Therefore, Four cyclodextrin formulations were prepared and characterized. Data analysis revealed the best formula (F2) showed a smallest particle size (15 nm), zeta potential (-37 mv), and higher yield percentages (95%) was selected for spem capacitation. Motile spermatozoa were separated from frozen-thawed semen by a swim-up procedure and capacitated in IVF-TALP medium with different formulae of NCD or CD or without treatments (control) and incubated for 3hours(hr) at 38°C and evaluated every one (hr) interval. Data analysis revealed that the formulation of cyclodextrin nanoparticles (F2<strong>)</strong> after (2hr) incubation in the media gave best effect on sperm capacitation and acrosme reaction (AR) and effect of sperm treated with NCD on fertilization rate was evaluated. The results showed that the proportion of Oocytes fertilized was increased significantly in F2 (60%) than in the control (35%), and cyclodextrin group (50%) groups (<em>p</em>&lt;0.05). It could be inferred from this investigation that cyclodextrin nanoparticles can be used for biomedical interventions in bovine spermatozoa. NCD improve sperm motility, viability, and (AR), also fertilization rate of sperm treated with NCD increase. So NCD gave positive effect on sperm functions during IVF. </p>

Asynchrony between human cumulus-corona cell complex and oocyte maturation after human menopausal gonadotropin treatment for in vitro fertilization

1984 ◽  
Vol 42 (3) ◽  
pp. 366-372 ◽  
Author(s):  
Neri Laufer ◽  
Basil C. Tarlatzis ◽  
Alan H. DeCherney ◽  
Joyce T. Masters ◽  
Florence P. Haseltine ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Oocyte Maturation ◽  
Cell Complex ◽  
Human Menopausal Gonadotropin ◽  
Vitro Fertilization
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