scholarly journals Screening for some biological activities of cultured cordyceps neovolkiana

2017 ◽  
Vol 20 (K3) ◽  
pp. 106-112
Author(s):  
Chi-Dung Nguyen ◽  
Thu Huynh ◽  
Minh-Hiep Dinh
Keyword(s):  
Free Radical ◽  
Inhibitory Activity ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Proliferation Inhibition ◽  
Etoh Extract ◽  
Toxicity Effect ◽  
Biological Compounds ◽  
Ic50 Values

Cordyceps has been demonstrated to possess a myriad of biological compounds and effects. There are various strains of Cordyceps. In this study, we evaluated some biological activities of cultured Cordyceps neovolkiana extracts. The result exhibited that all C. neovolkiana extracts almost showed no toxicity effect on HepG2 cells at the concentration of 100 μg/ml. Besides, all C. neovolkiana extracts also were not α-glucosidase inhibitory activity at the concentration from 1000 to 8000 μg/ml. However, some extracts had ABTS• free radical scavenging potential with IC50 values between 4129.92 ± 25.12 and 4926.25 ± 41.01 μg/ml. In addition, at 200 μg/ml, the EtOH extract exhibited 64.57 ± 6.30 % (p<0.001) of PBMC proliferation inhibition. In conclusion, these data revealed biological activities of cultured C. neovolkiana, suggesting that further studies would be necessary.

scholarly journals Tacrine, Trolox and Tryptoline as Lead Compounds for the Design and Synthesis of Multi-target Agents for Alzheimer’s Disease Therapy

2017 ◽  
Vol 11 (1) ◽  
pp. 24-37 ◽  
Author(s):  
Gerard A. K. Teponnou ◽  
Jacques Joubert ◽  
Sarel F. Malan
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Free Radical ◽  
Inhibitory Activity ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Linker Chain ◽  
Chain Lengths ◽  
Free Radical Scavenging Activities

The versatile biological activities of tacrine, trolox and β-carboline derivatives make them promising lead structures for the development of multifunctional Alzheimer’s disease (AD) agents. Based on the topology of the active site of cholinesterases and other target proteins involved in the pathogenesis of AD, we have designed and synthesized tacrine-trolox and tacrine-tryptoline hybrids with various linker chain lengths. The hybrids containing the trolox moiety (8a-8d) showed moderate to high TcAChE inhibition (IC50: 17.37 - 2200 nM), eqBuChE inhibition (IC50: 3.16 – 128.82 nM) and free radical scavenging activities (IC50: 11.48 – 49.23 µM). The hybrids with longer linker chain lengths in general showed better ChE inhibitory activity. As expected, free radical scavenging activities were not significantly affected by varying linker chain lengths. The hybrid compound containing the tryptoline moiety linked with a 7 carbon spacer to tacrine (14) displayed the best AChE and BuChE inhibitory activity (IC50 = 17.37 and 3.16 nM). Docking experiments exhibited that compounds 8d and 14 were able to bind to both the CAS and PAS of TcAChE and eqBuChE, suggesting that they will be able to inhibit ChE induced Aβ aggregation. Novel multi-target agents that exhibit good ChE inhibition (8d and 14) and anti-oxidant (8d) activity were identified as suitable candidates for further investigation.

scholarly journals Antiradical and Cytotoxic Activities of Varying Polarity Extracts of the Aerial Part ofEuphorbia hirtaL.

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Shanmugapriya Perumal ◽  
Roziahanim Mahmud ◽  
Suthagar Pillai Piaru ◽  
Wei Cai Lee ◽  
Surash Ramanathan
Keyword(s):  
Linoleic Acid ◽  
Free Radical ◽  
Aerial Part ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Free Radical Scavenging ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Uv Spectrophotometry ◽  
Etoh Extract

Euphorbia hirtais a well-known ethnomedicinal plant with diverse biological activities. The aim of the present study is to investigate the antiradical activities of various solvent extracts of the aerial part ofE. hirtaas well as to determine the possible cytotoxicity of these extracts. The aerial part ofE. hirtawas extracted with different solvent systems in order to increase polarity. The solvents used were hexane, dichloromethane (DCM), ethyl acetate (EA), ethanol (EtOH), and methanol (MeOH). The contents of total phenols and total flavonoids were analyzed by UV spectrophotometry, whereas the potential free radical-scavenging activities of the extracts were evaluated using the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH), theβ-carotene-linoleic acid bleaching system, and reducing power. The EtOH extract exhibited the highest total phenolic content (237.9±2.26 mg GAE/g), and DCM extract scored the highest total flavonoid content (121±0.15 mg CE/g). The MeOH extract showed a potent free radical-scavenging activity as evidenced by low EC50at 42.81 µg/mL. Interestingly, the EtOH extract demonstrated the highest reducing power activity with EC50value of 6.18 µg/mL. Inβ-carotene-linoleic acid assay, oxidation of linoleic acid was effectively inhibited by DCM extract with96.15±0.78%. All the extracts showed no cytotoxic activity against Vero cells.

Natural terpenes as substances with multidirectional biological activities

2019 ◽  
Vol 6 ◽  
pp. 43-50
Author(s):  
Karolina A. Wojtunik-Kulesza ◽  
Monika Waksmundzka- Hajnos
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Essential Oils ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Lemon Balm ◽  
The World ◽  
Alzhiemer's Disease ◽  
Main Components

Terpenes, secondary metabolites, are considered by scientists around the World. Multidirectional biological activities of these substances encourage researchers to their detail analysis towards numerous diseases such as neurodegenerative or metabolic. In the presented paper, authors have focused on Alzheimer’s disease and diabetes as potential direction of terpenes’ activity. According to available literature, terpenes exhibit satisfactory antioxidant activity and ability to inhibition AChE, BuChE (Alzhiemer’s disease) and α-amylase, α-glucosidase (diabetes). Basis of the presented research are both essential oils obtained from plants such as salvia, rosemary, basil and lemon balm being one of the main source of natural terpenes, and single compounds. Special attention is paid to monoterpenes as the main components of numerous essential oils. Among terpenes presented in the paper, activity of ocimene, carvone, pulegone, α-phellandrene exhibiting activity towards both free radical scavenging as well as AChE and BuChE inhibition, and α-pinene, citral, limonene that revealing inhibitory activity towards α-amylase and α-glucosidase should be emphasized.

scholarly journals Chemical profile and scavenging activity of four extracts from Hibiscus sabdariffa L. calyx

2020 ◽  
Author(s):  
Sakina Yagi ◽  
Zeinab T.E Hussain
Keyword(s):  
Free Radical ◽  
Solvent Extraction ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Bioactive Molecules ◽  
Hibiscus Sabdariffa ◽  
Scavenging Activity ◽  
Etoh Extract

Abstract Objective Hibiscus sabdariffa L., also known as roselle and karkadeh, is commonly used in traditional medicine and has many applications on food, pharmaceutical and cosmetic industries. More than 100 cultivars of H. sabdariffa are found. Despite the fact that Sudan is considered as one of the principal producer of H. sabdariffa there has been limited systematic study on the efficiency of different solvent extraction on its bioactive molecules. Therefore, this study was designed to investigate the chemical constituents of H. sabdariffa calyx using acetone, ethanol (EtOH), 50% EtOH and water as extraction solvents and their impact on the DPPH free radical scavenging activity. Results Results showed that the total polyphenol, flavonoids and vitamin C contents of the dried calyces were significantly (P < 0.05) affected by the solvent extraction used. HPLC analysis using 16 standards revealed the presence of 11–13 compounds in the four extracts with the 50% EtOH extract recovered the highest concentrations of identified compounds. Moreover, the 50% EtOH extract revealed highest DPPH free radical scavenging activity (IC50 1.544 ± 0.3 µg/mL). Based on these results, maceration the calyx on 50% EtOH was therefore suggested for optimal extraction of bioactive compounds with high antiradical activity from H. sabdariffa calyx.

scholarly journals Evaluation of Free Radical Scavenging Activity in Ethanolic Extract from Promising Accessions of Curcuma aeruginosa RoxB.

Molekul ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. 133 ◽  
Author(s):  
Waras Nurcholis ◽  
Nurul Khumaida ◽  
Muhamad Syukur ◽  
Maria Bintang
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Pharmaceutical Products ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Dpph Radical ◽  
Ic50 Values

This study evaluated the free radical scavenging activity in ethanolic extracts from 20 accessions of Curcuma aeruginosa. The radical scavenging activity of the extract accessions was investigated with 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical. IC50 values for DPPH radical scavenging activity ranged from 89.81 to 505.65 µg mL-1. Based on IC50 values, twenty accessions of C. aeruginosa can be divided into three groups: strong (two accessions); moderate (seventeen accessions); and low (one accession) of DPPH scavenger. Sukoharjo (SH) and Muara Bungo (MB) showed promising accessions for antioxidant potential, thus these accessions important to selection for future breeding program in pharmaceutical products.

scholarly journals Antioxidant and α-Glucosidase Inhibitory Constituents from Hornstedtia Species of Malaysia

2015 ◽  
Vol 10 (9) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Siti Ernieyanti Hashim ◽  
Hasnah Mohd Sirat ◽  
Khong Heng Yen ◽  
Intan Safinar Ismail ◽  
Siti Nurulhuda Matsuki
Keyword(s):  
Free Radical ◽  
Inhibitory Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Spectroscopic Techniques ◽  
Inhibition Activity ◽  
Dpph Assay ◽  
Glucosidase Inhibitors ◽  
Antioxidant Agent ◽  
First Time

Seven compounds were isolated from the n-hexane and chloroform extracts of the flowers and leaves of four Hornstedtia species and their structures were identified using spectroscopic techniques as 3,7,4′-trimethylkaempferol (1), 3,7-dimethylkaempferol (2), 7,4′-dimethylkaempferol (3), 3,5-dimethylkaempferol (4), 3-methylkaempferol (5), stigmast-4-en-3-one (6), and 6-hydroxy-stigmast-4-en-3-one (7). Compounds 1 to 7 were isolated from these species for the first time. They were assayed for free radical scavenging and α-glucosidase inhibition activities. The DPPH assay showed that 3-methylkaempferol (5) was the most potent antioxidant agent with an IC50 value 78.6 μM, followed by 7,4′-dimethylkaempferol (3) (IC50 = 86.1 μM). For α-glucosidase inhibition activity, 3-methylkaempferol (5) exhibited significant inhibitory activity with an IC50 value 21.0 μM. The present study revealed that Hornstedtia species have potential activities as antioxidant and α-glucosidase inhibitors.

Synthesis, Characterization, Free-radical Scavenging Capacity and Antioxidant Activity of Novel Series of Hydrazone, 1,3,4-oxadiazole and 1,2,4- triazole Derived from 3,5-dimethyl-1H-pyrazole

2019 ◽  
Vol 16 (7) ◽  
pp. 712-720 ◽  
Author(s):  
Khalid Karrouchi ◽  
Saad Fettach ◽  
Smaail Radi ◽  
El bekkaye Yousfi ◽  
Jamal Taoufik ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Radical Scavenging Capacity ◽  
Scavenging Capacity ◽  
Anti Cancer ◽  
Ic50 Values

Background: Pyrazole is an important class of heterocyclic compound, has been shown to exhibit diverse biological and pharmacological activities such as anti-inflammatory, anti-cancer, antioxidant, etc. Methods: In this study, a series of novel 3,5-dimethyl-1H-pyrazole derivatives containing hydrazine 4a-l have been synthesized via the reaction of the 2-(3,5-dimethyl-1H-pyrazol-1-yl)acetohydrazide. All synthesized compounds have been tested for their in vitro antioxidant activities via utilization of 1,1-biphenyl-2-picrylhydrazyl (DPPH) as a free radical scavenging reagent. Results: The data reported herein indicates that compound 4k showed potential radical scavenging capacity and compounds 4f and 4g exhibited best activity for the iron binding while comparing with positive controls. Conclusion: Good activity was noted for some compounds. In particular, compound 4k showed the highest antioxidant activity with IC50 values of 22.79 ± 3.64 and 1.35 ± 0.66 μg/mL in the DPPH and ABTS tests, respectively.

scholarly journals STUDIES ON THE PHYTOCHEMICAL SCREENING AND FREE RADICAL SCAVENGING POTENTIALS OF SOLANUM NIGRUM LEAVES EXTRACT

2016 ◽  
Vol 9 (6) ◽  
pp. 316
Author(s):  
Sriram Prasath
Keyword(s):  
Free Radical ◽  
Mineral Content ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Free Radical Scavenging ◽  
Solanum Nigrum ◽  
Phytochemical Analysis ◽  
Sodium And Potassium

ABSTRACTSolanum nigrum is a paramount in medicinal perspective and belongs to family Solanacea. From different parts of the plant significant pharmacological and biological activities have been reported previously.  In the present study, the ethanolic extract of leaves was investigated for phytochemical analysis, vitamin and mineral content. Phytochemical analysis of the leaves revealed the presence of phenols, alkaloids, flavonoids, glycosides, saponins, tannins, phytosterols and triterpenoides. The leaves extract was found to contain appreciable amounts of flavonoids and phenols. The extract showed the presence of vitamins such as ascorbic acid, folic acid, and niacinamide. It has been found that the leaves of Solanum nigrum is rich in minerals like copper, magnesium, manganese, vanadium, chromium, calcium, zinc, sodium, and potassium. The antioxidant (free radical scavenging) activity of the extract was determined against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) ABTS, Nitric oxide(NO) and Superoxide Scavenging assays. Solanum nigrum leaves extract was found to be antioxidant in nature which is evident from DPPH, ABTS, NO, Superoxide radical scavenging assays. These findings suggest that Solanum nigrum possess biologically important phytoconstituents, significant amounts of vitamins and minerals. IN addition, S. nigrum leaves extract exert free radical scavenging potential in vitro.Key words: Medicinal plants; Solanum nigrum leaves; phytoconstiuents;Antioxidant.  

Sign in / Sign up

Export Citation Format

Share Document