Micropropagation of Musa balbasiana (BBB group)

The banana domestic market as well as global market are growing. Musa balbasiana (BBB group) is now one of the preferred banana types; however, this banana group is facing up to risk of degeneration and disease. Therefore, the micropropagation of banana to provide genetic stability, disease-free seedlings, and enriching the types of bananas in the market is essential. In this study, we have completed the process of Musa balbasiana (BBB group) micropropagation. The study results showed that the samples were sterilized with NaOCl 3% solution in 12-15 minutes depending on the size and age of the samples, buds were regenerated on Murashige and Skoog medium (MS) added 0.5 mg/L 1-phenyl-3 (1,2,3 thiadiazol-5-yl) (TDZ). MS medium supplemented with 1 mg/L benzylaminopurine (BA) and 0.25 mg/L kinetin made high efficiency shoot initation (10.700 ± 0.135 buds/sample) and the shoot height at the day of 20 was 3.023 ± 0.018 cm. Musa balbasiana (BBB group) had been the most induced roots on MS medium without plant growth regulators with 4.533 ± 0.058 roots/shoot and the roots were 2.433 ± 0.067 cm in length. Complete seedlings (with roots, stems, and leave) were transferred to the nursery and planted on sand with the highest survival rate of 91.1 %. After two weeks, the survival plants were moved to grown on clean soil for the best growth and development.

Download Full-text

Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application

Agronomy ◽

10.3390/agronomy11020320 ◽

2021 ◽

Vol 11 (2) ◽

pp. 320

Author(s):

Nisar Ahmad Zahid ◽

Hawa Z.E. Jaafar ◽

Mansor Hakiman

Keyword(s):

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Zingiber Officinale ◽

Naphthaleneacetic Acid ◽

Ms Medium ◽

Zingiber Officinale Roscoe ◽

Planting Materials ◽

Disease Free

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.

Download Full-text

In vitro growth and development of Dendrobium hybrid orchid

Bangladesh Journal of Agricultural Research ◽

10.3329/bjar.v35i3.6457 ◽

1970 ◽

Vol 35 (3) ◽

pp. 507-514 ◽

Cited By ~ 1

Author(s):

H Khatun ◽

MM Khatun ◽

MS Biswas ◽

MR Kabir ◽

M Al-Amin

Keyword(s):

Growth Regulators ◽

Growth And Development ◽

Ms Medium ◽

Fresh Weight ◽

Maximum Weight ◽

Shoot Height ◽

Single Shoot ◽

Number Of Leaves ◽

Regenerated Plantlets

The experiment was conducted to investigate the combined effect of different plant growth regulators and charcoal supplementation in MS medium on growth and development of plantlets regenerated from protocorm like bodies (PLBs) of hybrid orchid. The combination of BAP + NAA, BAP + IAA, BAP + IBA, and IAA + IBA at different concentrations with charcoal supplementation was studied. The result revealed that the use of different growth regulators had significant effect on different parameters studied. The maximum weight of PLBs (5.123 g) was obtained from the combination of BAP + IBA at 1.0 mg/l each. The highest shoot height (3.239 cm) and maximum number of rooted plantlets (4.473) was obtained from 1.0 mg/l each of BAP + NAA combination. The maximum number of leaves (3.490) and the maximum length of leaves (1.946 cm) were obtained from 1.0 mg/l each of BAP + IBA and the highest leaf width (1.166 cm) was obtained from 0.5 mg/l BAP +1.0 mg/l IBA combination. The highest root length was obtained from 0.5 mg/l each of BAP + IAA and the maximum number of regenerated plantlets (20) was obtained from 0.5 mg/l IAA + 1.0 mg/l IBA combination. However, the maximum fresh weight of single shoot (0.219 g) and the maximum number of roots per plantlet (6.300) was obtained from 1.0 mg/I each of IAA + IBA combination. Keywords: Dedrobium; orchid; hybrid; In vitro growth. DOI: 10.3329/bjar.v35i3.6457Bangladesh J. Agril. Res. 35(3) : 507-514

Download Full-text

Triacontanol Promotes the Fruit Development and Retards Fruit Senescence in Strawberry: A Transcriptome Analysis

Plants ◽

10.3390/plants9040488 ◽

2020 ◽

Vol 9 (4) ◽

pp. 488

Author(s):

Qianqian Pang ◽

Xueqin Chen ◽

Jinhua Lv ◽

Teng Li ◽

Jinggui Fang ◽

...

Keyword(s):

Plant Growth ◽

Fruit Development ◽

Growth And Development ◽

High Efficiency ◽

Low Cost ◽

Sugar Content ◽

Regulation Mechanism ◽

Cellular Processes ◽

Go Enrichment ◽

Related Enzyme

Triacontanol (TA) is a non-toxic, pollution-free, low-cost, high-efficiency, broad-spectrum plant growth regulator that plays an important role in plant growth and development, but its regulation mechanism of strawberry (Sweet charlie, Fragaria × ananassa Duch.) fruit development is still unclear. In this study, we showed that TA treatment (50 μM) could promote fruit development by up-regulating factors related to fruit ripening-related growth and development. TA increased fruit sugar content and anthocyanin accumulation, and many stress-related enzyme activities. In the meantime, Illumina RNA-Seq technology was used to evaluate the effect of TA treatment on strawberry fruit senescence. The results showed that 9338 differentially expressed genes (DEGs) were obtained, including 4520 up-regulated DEGs and 4818 down-regulated DEGs. We performed gene ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of these DEGs. The results showed that TA treatment caused changes in transcript levels related to cellular processes, hormones and secondary metabolism, such as DNA metabolic processes, flavonoid synthesis, and plant hormone signal transduction. Bioinformatics analysis showed that many transcription factors were related to fruit maturity. Taken together, this study will provide new insights into the mechanism of strawberry development and postharvest response to TA treatment.

Download Full-text

Distribution of Trans-Anethole and Estragole in Fennel (Foeniculum vulgare Mill) of Callus Induced from Different Seedling Parts and Fruits

Notulae Scientia Biologicae ◽

10.15835/nsb315422 ◽

2011 ◽

Vol 3 (1) ◽

pp. 79-86 ◽

Cited By ~ 2

Author(s):

Abd El-Moneim Mohamed Radwan AFIFY ◽

Hossam Saad EL-BELTAGI ◽

Anwer Abd El-Aziz HAMMAMA ◽

Mahassen Mohamed SIDKY ◽

Omneya Farouk Ahmed MOSTAFA

Keyword(s):

Essential Oil ◽

Plant Growth ◽

Human Consumption ◽

Dichlorophenoxyacetic Acid ◽

Ms Medium ◽

Foeniculum Vulgare ◽

Local Cultivar ◽

Murashige And Skoog Medium ◽

Different Types ◽

2,4 Dichlorophenoxyacetic Acid

In the present study, seeds from local cultivar of fennel were germinated on Murashige and Skoog medium (MS) without plant growth regulators. Different types of explants from the growing seedling such as cotyledonal leaves, hypocotyls, epicotyls and roots were cultured on MS medium, contained different concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) either alone or with kinetin. Differential responses in the essential oil constituents were observed in the induction and development of callus. The major components of essential oils includes estragole, trans-anethole, limonene and fenchone were studied under different conditions to find out the best methods which could be used to reduce the amount of estragole (not favorite for human consumption) and increase the amount of trans-anethole.

Download Full-text

Evaluation of the Behavior of Some Genotypes of Salix sp. in the Conditions of Sandy Soils

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Agriculture ◽

10.15835/buasvmcn-agr:003017 ◽

2018 ◽

Vol 75 (1) ◽

pp. 44

Author(s):

Marin SOARE ◽

Paula IANCU ◽

Elena BONCIU ◽

Ion NICOLAE ◽

Luminita BUSE DRAGOMIR ◽

...

Keyword(s):

Survival Rate ◽

Plant Growth ◽

Vegetative Growth ◽

Growth And Development ◽

Sandy Soils ◽

Stem Height ◽

Short Rotation ◽

Hydric Stress ◽

Main Factors ◽

Comparative Analyse

The aim of this study was to make a comparative analyse of nine willow genotypes (seven Romanian and two Swedish clones) in order to test them for short rotation coppice. Researches have been carried out in the sandy soils from Southern Romania, over a period of two years of vegetation. There were made determinations as concern plants growth and development and were referring to survival rate, stem height, number of shoots/plant and stem diameter at the base. Hydric stress is one of the main factors of limiting plant growth on this type of soil and the experimented willow genotypes have reacted differently. The obtained results have shown the fact that the largest vegetative growth was recorded in Pesred and Inger genotypes. It also emphasizes with good results in terms of the quantity of biomass RO 1077, Fragisal and Robisal genotypes. Based on the obtained results it can appreciate that Salix sp. represents a solution of utilize the sandy soils which have low fertile and obtaining considerable amounts of biomass, in the conditions of ensuring of water and nutritive resources.

Download Full-text

In vitro propagation from nodal segments of Lippia origanoides (chemotype A)

Ciência Rural ◽

10.1590/0103-8478cr20210323 ◽

2022 ◽

Vol 52 (7) ◽

Author(s):

Julián Sebastián Ramírez Moreno ◽

Sergio Andrés Vega Porras ◽

Roosevelt Humberto Escobar ◽

Elena E. Stashenko ◽

Jorge Luis Fuentes Lorenzo ◽

...

Keyword(s):

Plant Growth ◽

Gibberellic Acid ◽

Growth Regulators ◽

In Vitro Propagation ◽

Callus Formation ◽

Nodal Segments ◽

Ms Medium ◽

Shoot Number ◽

Murashige And Skoog Medium

ABSTRACT: This research described an efficient micropropagation protocol for Lippia origanoides (Verbenaceae). Sterile seeds were used to obtain germinated seedlings in Murashige and Skoog medium (MS) supplemented with sucrose and agar. The nodal segments obtained from seedlings were grown on MS medium supplemented with different concentrations of gibberellic acid (GA), benzylaminopurine (BAP) and 1-naphthalenacetic acid (NAA) with BAP. The callus induction, shoots length, shoots number and root length, were analyzed. The treatments showed high percentage of callus formation at 0.5 to 1.5 mg L-1 of BAP alone or in combination with NAA (0.1 mg L-1). The highest value of shoot number per nodal segments was obtained at 1.5 mg L-1 of BAP (4.3 ± 0.8). The obtained plantlets were better rooted in vitro in the absence of plant growth regulators (PGRs) and they showed acclimatization rate of 90%. We reported a protocol for in vitro propagation and acclimatization of L. origanoides for A chemotypes from Colombia.

Download Full-text

EFFECT OF PLANT GROWTH REGULATORS ON SOMATIC EMBRYOGENESIS OF EGGPLANT (SOLANUM MELONGENA L.)

Science and Technology Development Journal ◽

10.32508/stdj.v12i17.2369 ◽

2009 ◽

Vol 12 (17) ◽

pp. 71-80

Author(s):

Nam Ngoc Trinh ◽

Sanh Du Nguyen

Keyword(s):

Somatic Embryogenesis ◽

Survival Rate ◽

Plant Growth ◽

Plant Growth Regulators ◽

Growth Regulators ◽

Callus Induction ◽

Somatic Embryos ◽

Solanum Melongena ◽

Ms Medium

On the MS medium containing only 2,4-D, callus induction was inhibited. Moreover, these calli were friable and turned brown after two weeks of culture. the three-week old calli were then transferred to the MS medium containing NAA 0.5 mg/l and kinetin 1 mg/l. The somatic embryos with globular shape appeared after 10 days of culture, while the heart shape, torpedo_shape and cotyledonary_shape embryos appeared successively after 15 days of culture. The abnormal embryos occupied at a rate of 34.3% and rarely germinated to plantlets. On the MS medium without plant growth regulators or only with NAA, somatic embryos could not be induced. On MS medium supplemented with ethephon 3 mg/l somatic embryogenesis from calli was inhibited. Plantlets derived from the eggplant somatic embryos had a survival rate up to 95% when transferred to the pots.

Download Full-text

INDUKSI KALUS DAN TUNAS DARI EKSPLAN PUCUK BROKOLI (Brassica oleracea L. sub var. italica Planch) PADA MEDIUM MS YANG DIBERIKAN NAA DAN BAP

EUGENIA ◽

10.35791/eug.19.1.2013.8377 ◽

2013 ◽

Vol 19 (1) ◽

Author(s):

W. Tilaar ◽

S. Tulung

Keyword(s):

Brassica Oleracea ◽

Research Design ◽

Callus Formation ◽

Shoot Formation ◽

Combination Effect ◽

Ms Medium ◽

Shoot Height ◽

Randomized Design ◽

Murashige And Skoog Medium ◽

Brassica Oleracea L

ABSTRACT The research aimed was to know the combination effect of NAA and BAP to callus and shoots induction of the explants bud of broccoli on Murashige and Skoog Medium. The research design used Completed Randomized Design in Factorial, which consisted of two treatments combination, i.e: NAA 0; 0,1 ; 1 ppm and BAP 0 ; 1; 2; 3; 4; 5; ppm. Each treatmen was repeated in three times. The observed variables were time of callus formation, time of shoot formation, high of shoot, and total shoot added to MS: Analisys of Varians. The result showed that combination of NAA and BAP influenced significantly the shoot height and the time of shoot formation however not to the total shoot of explant bud. Combination of NAA 1 ppm and BAP 3 ppm was excellent for height shoot however for the number of shoot was combination of NAA 0,1 ppm and BAP 5 ppm, and the time of shoot was on BAP 2 ppm. Callus was not formed on combination of NAA and BAP. Keywords : broccoli, NAA and BAP, MS medium ABSTRAK Tujuan penelitian ini adalah untuk mengetahui pengaruh kombinasi NAA dan BAP terhadap induksi kalus dan tunas dari eksplan pucuk brokoli yang ditumbuhkan pada media MS. Rancangan yang digunakan adalah Rancangan Faktorial dalam Rancangan Acak Lengkap terdiri dari dua kombinasi perlakuan yaitu NAA dengan konsentrasi 0 ; 0,1 ; 1 ppm dan BAP 0 ; 1 ; ; 2 ; 3 ; 4 : 5 ppm. Setiap perlakuan diulang tiga kali. Variabel yang diamati : waktu terbentuk kalus, waktu terbentuk tunas, tinggi tunas, dan jumlah tunas. Analisis yang digunakan yaitu Analisis Ragam. Hasil penelitian menunjukan bahwa kombinasi NAA dengan BAP yang ditambahkan ke media MS, berbeda nyata pengaruhnya terhadap tinggi tunas dan waktu bertunas tetapi tidak berbeda pengaruhnya terhadap jumlah tunas dari eksplan pucuk. Kombinasi terbaik untuk tinggi tunas adalah NAA 1 ppm dengan BAP 3 ppm, sedangkan pada jumlah tunas adalah NAA 0,1 ppm dengan BAP 5 ppm, dan waktu berbertunas adalah pada BAP 2 ppm. Kalus tidak terbentuk pada kombinasi perlakuan NAA dan BAP. Kata kunci : brokoli, NAA dan BAP, Medium MS

Download Full-text

Tissue culture studies on Cymbidium ensifolium (L.) Swartz

Journal of Applied and Advanced Research ◽

10.21839/jaar.2019.v4i4.70 ◽

2019 ◽

pp. 104-107

Author(s):

T. Ramesh ◽

P. Renganathan ◽

M. Prabhakaran

Keyword(s):

Tissue Culture ◽

Acetic Acid ◽

Survival Rate ◽

Seed Germination ◽

Plant Growth ◽

Ms Medium ◽

Nutrient Media ◽

Culture Studies ◽

Half Strength ◽

Cymbidium Ensifolium

An efficient protocol for seed germination and micropropagation of Cymbidium ensifolium (L.) Swartz. was established. Four nutrient media were used for seed germination and early protocorm development: Murashige and Skoog (MS), half –strength MS, Knudson ‘C’ (KC), and Vasin and Went (VW); combinations and alone of four plant growth regulators i.e. 6-benzylaminopurine (BAP), kinetin (KN), α-napthalene acetic acid (NAA), and indole-3-butyric acid (IBA) were studied. MS medium was found as most ideal for seed germination (98±0.48) and lowest in VW (71.12 ±0.42). 3 months old protocorm were sub cultured on fresh MS medium supplemented with different concentrations of BAP, KN, NAA, and IBA alone and in combination. After 30 days highest secondary protocorms (21.25±0.63) were observed in MS medium containing BAP (4.0μM). MS medium supplemented with 8μM IBA induced the maximum roots per shoot. After 16 days of transfer to green house the survival rate was 88%.

Download Full-text

Mechanisms of ethylene biosynthesis and response in plants

Essays in Biochemistry ◽

10.1042/bse0580061 ◽

2015 ◽

Vol 58 ◽

pp. 61-70 ◽

Cited By ~ 11

Author(s):

Paul B. Larsen

Keyword(s):

Plant Growth ◽

Growth And Development ◽

Ethylene Biosynthesis ◽

Unsaturated Hydrocarbon ◽

Flower Senescence ◽

Detailed Knowledge ◽

Plant Growth And Development ◽

Step Process ◽

Ethylene Response ◽

Ethylene Signalling

Ethylene is the simplest unsaturated hydrocarbon, yet it has profound effects on plant growth and development, including many agriculturally important phenomena. Analysis of the mechanisms underlying ethylene biosynthesis and signalling have resulted in the elucidation of multistep mechanisms which at first glance appear simple, but in fact represent several levels of control to tightly regulate the level of production and response. Ethylene biosynthesis represents a two-step process that is regulated at both the transcriptional and post-translational levels, thus enabling plants to control the amount of ethylene produced with regard to promotion of responses such as climacteric flower senescence and fruit ripening. Ethylene production subsequently results in activation of the ethylene response, as ethylene accumulation will trigger the ethylene signalling pathway to activate ethylene-dependent transcription for promotion of the response and for resetting the pathway. A more detailed knowledge of the mechanisms underlying biosynthesis and the ethylene response will ultimately enable new approaches to be developed for control of the initiation and progression of ethylene-dependent developmental processes, many of which are of horticultural significance.

Download Full-text