scholarly journals The presence of cellulose-degrading microorganisms in Perisesarma eumolpe crab’s stomach at opened areas of Can Gio mangrove forest

Author(s):  
My Ngoc Diem Tran ◽  
Nhan Trong Nguyen

The study was conducted to determine the presence of cellulose-degrading microorganisms which were living in the stomach of Perisesarma eumolpe crabs. P. eumolpe were collected in the gap of Can Gio mangrove caused by Durian typhoon in 2006. The study identified 520 microorganism samples from 30 stomach samples Cellulose degrading microbacteria were isolated on three types of nutrient media: meat extract – peptone, Gause and Czapek – Dok with CMC. These 520 microbial samples included of 496 aerobic bacteria, 7 actinomycetes and 0 mold samples. The number of cellulose degrading microorganisms was also accounted for 46% of total (240 samples). Among these, there were 24 bacteria strains and 5 actinomycetes strains which degradated cellulose based on the difference in the colony and the cell shape. The average microbial density per crab gut was approximately 0.66 ×105 to 6.6 ×105 cell/mL. In addition, the results showed that cellulose degrading microorganism groups have importantly contributed to the food sources for P. eumolpe during their living in the mangrove forest.

2007 ◽  
Vol 42 (3) ◽  
pp. 135-152 ◽  
Author(s):  
Quang Ngo Xuan ◽  
Ann Vanreusel ◽  
Nguyen Vu Thanh ◽  
Nic Smol

2020 ◽  
Vol 9 (2) ◽  
pp. 167-174
Author(s):  
Erik Wijaya Kusuma ◽  
Ria Azizah Tri Nuraini ◽  
Retno Hartati

ABSTRAK : Desa Kaliwlingi dan Sawojajar mempunyai kawasan hutan mangrove dengan karakteristik berbeda. Kawasan hutan mangrove Kaliwlingi menjadi ekowisata mangrove sejak tahun 2016, sedangkan di Sawojajar merupakan kawasan mangrove alami. Perbedaan fungsi tersebut diduga dapat mempengaruhi keanekaragaman hayati yaitu salah satunya adalah gastropoda. Tujuan dari penelitian ini adalah untuk mengetahui struktur komunitas Gastropoda di ekosistem mangrove Desa Kaliwlingi dan Sawojajar, Kab.Brebes, Jawa Tengah. Pengambilan sampel dilakukan pada bulan Juni sampai Juli 2018. Penentuan titik sampling menggunakan metode purposive pada 3 stasiun dengan tiga kali pengulangan. Hasil penelitian menunjukan terdapat 2 subkelas yaitu Pulmonata dan Prosobranchiata, dengan 3 famili dan  9 spesies gastropoda dari 3 famili, yaitu Elliobidae; Cassidula aurisfelis (Elliobidae), C. nucleus (Elliobidae), Littoraria articulate (Littorinidae), L. carinifera (Littorinidae), L. melanostoma (Littorinidae), L. scabra (Littorinidae), Telescopium telescopium (Potamididae), Terebralia obtuse (Potamididae), T. palustris (Potaminidae).  Nilai kelimpahan rata-rata gastropoda Desa Kaliwlingi dan Desa Sawojajar masing-masing 20,28 dan 16,36 Ind/m². Indeks Keanekaragaman (H’) gastropoda di kawasan mangrove Desa Kaliwlingi dan Desa Sawojajar termasuk ke dalam kategori rendah ke tinggi, sedangkan indeks keseragamannya  berkategori rendah.  Tidak ada jenis gastropod yang mendominasi di kawasan mangrove Kaliwlingi dan Sawojajar, dengan pola sebaran gastropoda mengelompok.ABSTRACT: Kaliwlingi and Sawojajar villages have mangrove forest areas with different characteristics. The Kaliwlingi mangrove forest area has been established as mangrove ecotourism since 2016, while in Sawojajar it is a natural mangrove area. The difference in function is thought to affect biodiversity, one of which is gastropods. The purpose of this study was to determine the structure of the Gastropoda community in the mangrove ecosystems of Kaliwlingi and Sawojajar Villages, Kab. Brebes, Central Java. Sampling was conducted in June to July 2018. Determination of the sampling points using purposive methods at 3 stations with three repetitions. The results showed that there were 2 subclasses, namely Pulmonata and Prosobranchiata, with 3 families and 9 gastropod species from 3 families, namely Elliobidae; Cassidula aurisfelis (Elliobidae), C. nucleus (Elliobidae), Littoraria articulate (Littorinidae), L. carinifera (Littorinidae), L. melanostoma (Littorinidae), L. scabra (Littorinidae), Telescopium telescopium (Potamebidide) ), T. palustris (Potaminidae). The mean abundance of gastropods in Kaliwlingi Village and Sawojajar Village were 20.28 and 16.36 Ind / m² respectively. Diversity Index (H ') of gastropods in the mangrove areas of Kaliwlingi Village and Sawojajar Village are in the low to high category, while the uniformity index is categorized as low. There is no type of gastropod that dominates in the Kaliwlingi and Sawojajar mangrove areas, and gastropod distributed as a grouped.  


2021 ◽  
Vol 17 (11) ◽  
pp. e1010020
Author(s):  
Delphine Payros ◽  
Henar Alonso ◽  
Wladimir Malaga ◽  
Arnaud Volle ◽  
Serge Mazères ◽  
...  

Mycobacterium tuberculosis, the main causative agent of human tuberculosis, is transmitted from person to person via small droplets containing very few bacteria. Optimizing the chance to seed in the lungs is therefore a major adaptation to favor survival and dissemination in the human population. Here we used TnSeq to identify genes important for the early events leading to bacterial seeding in the lungs. Beside several genes encoding known virulence factors, we found three new candidates not previously described: rv0180c, rv1779c and rv1592c. We focused on the gene, rv0180c, of unknown function. First, we found that deletion of rv0180c in M. tuberculosis substantially reduced the initiation of infection in the lungs of mice. Next, we established that Rv0180c enhances entry into macrophages through the use of complement-receptor 3 (CR3), a major phagocytic receptor for M. tuberculosis. Silencing CR3 or blocking the CR3 lectin site abolished the difference in entry between the wild-type parental strain and the Δrv0180c::km mutant. However, we detected no difference in the production of both CR3-known carbohydrate ligands (glucan, arabinomannan, mannan), CR3-modulating lipids (phthiocerol dimycocerosate), or proteins in the capsule of the Δrv0180c::km mutant in comparison to the wild-type or complemented strains. By contrast, we established that Rv0180c contributes to the functionality of the bacterial cell envelope regarding resistance to toxic molecule attack and cell shape. This alteration of bacterial shape could impair the engagement of membrane receptors that M. tuberculosis uses to invade host cells, and open a new perspective on the modulation of bacterial infectivity.


Development ◽  
1987 ◽  
Vol 101 (2) ◽  
pp. 237-246 ◽  
Author(s):  
G.L. Campbell

Cells from different levels in the anteroposterior axis of an abdominal segment of Oncopeltus were confronted by scraping away the strip of epidermis that separated these levels. The cells migrate over the wound and meet in the centre. The subsequent behaviour of the epidermal cells was followed by preparing whole mounts of integument at various times after confrontation. These operations may lead to cell division and an alteration in cell shape at the confrontation site. The intensity of the induced cell behaviour pattern depends on which levels in the segment are confronted and the evidence suggests that it is directly related to the magnitude of the difference in positional values between confronted cells. The results can be explained by a nonlinear gradient of positional values within the segment with a crowding of values in the posterior region. It is also shown that segment border formation requires the confrontation of cells with a near maximum possible difference in positional values.


2004 ◽  
Vol 61 (4) ◽  
pp. 552-562 ◽  
Author(s):  
Kerri Finlay ◽  
John C Roff

Abstract Many studies have focused on the consumption of specific prey items and clearance rates on natural food particles by copepod adults and copepodites, but few have addressed the feeding habits of the youngest stages, the nauplii. Because of the difference in size and shape between copepod developmental stages, corresponding differences in diet would be expected. This subject was addressed using a modification of a new method offering radiolabelled natural food particles (with 3H for heterotrophs and 14C for autotrophs) to nauplii and copepodites of the copepods in a temperate estuary. By measuring the uptake of the radiolabel by the copepods it was possible to determine clearance rates on different sizes and types of food particles. All copepods began feeding on food sources >2 μm in size, and feeding on heterotrophs by all species and stages was considerably lower than feeding on autotrophs. Overall, it appears that copepod nauplii have a comparable diet to the later stages in terms of food type and size and therefore it is unlikely that nauplii are a more efficient link between the microbial foodweb and the classical foodweb, at least in temperate estuarine waters.


2016 ◽  
Vol 380 ◽  
pp. 31-40 ◽  
Author(s):  
Luu Viet Dung ◽  
Nguyen Tai Tue ◽  
Mai Trong Nhuan ◽  
Koji Omori

2009 ◽  
Vol 12 (7) ◽  
pp. 72-78
Author(s):  
Hang Thi Gia Nguyen ◽  
Triet Tran ◽  
Tung Thanh Nguyen

This study investigated benthic diatom communities of mangrove forests at Can Gio Mangrove Biosphere Reserve, Ho Chi Minh City, Vietnam. Samples were collected on two substrates: mud surface and living root surface. Sampling locations were placed in four types of mangrove vegetation: mud flat (without vegetation), Rhizophora forests, mixed forest of Avicennia-Rhizophora and Avicennia forest along three tidal creeks during wet and dry seasons of 2004-2005. A total of 348 taxa were identified, of which 100 taxa belong to the Order Centrales and 238 belong to the Order Pentales. There were significant differences in benthic diatom species richness and species composition between two types of substrates and among different mangrove forest types. The difference between wet and dry season was not significant.


2021 ◽  
Vol 38 (3) ◽  
pp. 263-268
Author(s):  
Zafer Ceylan ◽  
Turgay Çetinkaya

The aim of the present study was to reveal the textural profile changes like hardness, cohesiveness, springiness, and adhesion supported by total mesophilic aerobic bacteria count (TMABc), pH, and some visual sensory characteristics in squid samples stored at 4 ºC. Three different Brookfield Texture Analyzer tools, named TA7, TA9, and TA18 were used to observe the textural changes more clearly. The difference of TMABc between the storage days reached from 4.32 log CFU/g to 6.32 log CFU/g. The hardness value of the squid samples, detected by TA18 and TA9 tools, increased while the hardness value obtained from TA7 was higher. The highest change in cohesiveness value in the squid samples was defined by the TA9 tool as ~63%. Once springiness values detected by TA9 were sharply decreased from 4.9 mm to 2.1 mm, those of TA7 and TA18 were slowly decreased. The most increase in adhesion value (0.08 mJ to 0.21 mJ) was obtained in the TA7 tool. Depending on the textural quality changes, the pH value was increased, and as visual and sensory, dark, or yellow spots were observed. The present study results revealed that especially the TA18 tool could be effectively used to determine the quality changes of the squid samples.


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