Cell behaviour during postembryonic pattern regulation in the insect abdomen (Oncopeltus fasciatus). II. Intrasegmental regulation

Development ◽  
1987 ◽  
Vol 101 (2) ◽  
pp. 237-246 ◽  
Author(s):  
G.L. Campbell
Keyword(s):  
Cell Division ◽  
Cell Shape ◽  
Abdominal Segment ◽  
Oncopeltus Fasciatus ◽  
Behaviour Pattern ◽  
Cell Behaviour ◽  
The Difference ◽  
Pattern Regulation ◽  
Different Levels ◽  
Whole Mounts

Cells from different levels in the anteroposterior axis of an abdominal segment of Oncopeltus were confronted by scraping away the strip of epidermis that separated these levels. The cells migrate over the wound and meet in the centre. The subsequent behaviour of the epidermal cells was followed by preparing whole mounts of integument at various times after confrontation. These operations may lead to cell division and an alteration in cell shape at the confrontation site. The intensity of the induced cell behaviour pattern depends on which levels in the segment are confronted and the evidence suggests that it is directly related to the magnitude of the difference in positional values between confronted cells. The results can be explained by a nonlinear gradient of positional values within the segment with a crowding of values in the posterior region. It is also shown that segment border formation requires the confrontation of cells with a near maximum possible difference in positional values.

Cell behaviour during postembryonic pattern regulation in the insect abdomen (Oncopeltus fasciatus). I. Regeneration of segment borders

Development ◽  
1987 ◽  
Vol 101 (2) ◽  
pp. 221-235 ◽  
Author(s):  
G.L. Campbell ◽  
P.M.J. Shelton
Keyword(s):  
Wound Healing ◽  
Cell Division ◽  
Abdominal Segment ◽  
Oncopeltus Fasciatus ◽  
Adjacent Segment ◽  
Special Population ◽  
Maximum Difference ◽  
Cell Behaviour ◽  
The Stability ◽  
Pattern Regulation

The confrontation of cells from the anterior region of an abdominal segment of Oncopeltus with those from the posterior region of the same or the adjacent segment results in the generation of a segment border. The behaviour of epidermal cells during this regulation is described. It consists primarily of cell division and transverse elongation of cells at the site of confrontation. This behaviour can be separated from any associated purely with wound healing because a similar-sized wound to that used to ablate the segment border, performed within the segment, does not result in any cell division or elongation. The results are consistent with the view that there is a discontinuity in positional values at the segment border. The stability of such a discontinuity and the regeneration of segment borders are discussed in terms of there being a special population of cells at the segment border that have the property of isolating other cells with the maximum difference in positional values.

scholarly journals Mode Shape-Based Damage Detection Method (MSDI): Experimental Validation

2021 ◽  
Vol 11 (10) ◽  
pp. 4589
Author(s):  
Ivan Duvnjak ◽  
Domagoj Damjanović ◽  
Marko Bartolac ◽  
Ana Skender
Keyword(s):  
Boundary Conditions ◽  
Damage Detection ◽  
Mode Shape ◽  
Experimental Validation ◽  
Detection Method ◽  
Dynamic Properties ◽  
Damage Index ◽  
Main Principle ◽  
The Difference ◽  
Different Levels

The main principle of vibration-based damage detection in structures is to interpret the changes in dynamic properties of the structure as indicators of damage. In this study, the mode shape damage index (MSDI) method was used to identify discrete damages in plate-like structures. This damage index is based on the difference between modified modal displacements in the undamaged and damaged state of the structure. In order to assess the advantages and limitations of the proposed algorithm, we performed experimental modal analysis on a reinforced concrete (RC) plate under 10 different damage cases. The MSDI values were calculated through considering single and/or multiple damage locations, different levels of damage, and boundary conditions. The experimental results confirmed that the MSDI method can be used to detect the existence of damage, identify single and/or multiple damage locations, and estimate damage severity in the case of single discrete damage.

scholarly journals Six ultraviolet minutes for cleaner operating theatres

2020 ◽  
Vol 30 (Supplement_5) ◽  
Author(s):  
R Bosco ◽  
S Gambelli ◽  
V Urbano ◽  
G Cevenini ◽  
G Messina
Keyword(s):  
Cross Sectional Study ◽  
Cross Sectional ◽  
Before And After ◽  
Operating Theatres ◽  
Cleaning Procedures ◽  
The Difference ◽  
Contamination Levels ◽  
Different Levels ◽  
Better Than ◽  
Final Cleaning

Abstract Background Sanitizing the operating theatres (OT) is important to minimize risk of post-operative infections. Disinfection procedures between one operation and another is less aggressive than final cleaning procedures, at the end of the day. Aim was assessing the difference of contamination: i) between different levels of disinfection; ii) before and after the use of a UVC Device (UVC-D). Methods Between December 2019/February 2020 a cross sectional study was conducted in OT in a real clinical context. 94 Petri dishes (PD) were used in 3 OT. Three different sanitation levels (SL1-3) were compared pre- and post-use of UVC-D: i) No cleaning after surgery (SL1); ii) after in-between cleaning (SL2); iii) after terminal cleaning (SL3). UVC-D was employed for 6 minutes, 3 minutes per bed side. PD were incubated at 36 °C and colony forming unit (CFU) counted at 48h. Descriptive statistic, Wilcoxon and Mann-Whitney tests were performed to assess the contamination levels in total, pre/post use of UVC-D, and between different sanitation levels, respectively. Results In total we had a mean of 3.39 CFU/PD (C.I. 2.05 - 4.74) and a median of 1 CFU/PD (Min. 0 - Max. 39), after UVC-D use we had a mean of 2.20 CFU/PD (C.I. 0.69 - 5.09) and a median of 0 CFU/PD (Min. 0 - Max. 133). The UVC-D led to a significant reduction of CFU (p < 0.001). Without UVC-D we had a significant CFU drop (p < 0.05) between SL1 and SL3. Using UVC-D, we observed significant reductions of contamination (p < 0.05) between SL3 and SL1. Comparing SL1 (median 0) post UVC-D use vs SL2 pre UVC-D use (median 0.5), and SL2 post UVC-D use (median 0) vs SL3 pre UVC-D use (median 1) we had a significant reduction of contamination (p < 0.05). Conclusions UVC-D improved environmental contamination in any of the three sanitation levels. Furthermore, the use of UVC-D alone was better than in-between and terminal cleaning. Although these encouraging results, the cleaning procedures executed by dedicated staff has to be considered. Key messages UVC are efficient to decrease contamination in operating theatres regardless of sanitation levels. The additional use of UVC technology to standard cleaning procedures significantly improves sanitation levels.

Insertion of vowels in English syllabic consonantal clusters pronounced by L1 Polish speakers

2021 ◽  
Vol 7 (1) ◽  
pp. 331-341
Author(s):  
Urszula Chwesiuk
Keyword(s):  
Language Learning ◽  
Teaching Methodology ◽  
English Studies ◽  
Vowel Reduction ◽  
Potential Influence ◽  
English System ◽  
The Difference ◽  
Practical Implications ◽  
Different Levels

Abstract The aim of this study was an attempt to verify whether Polish speakers of English insert a vowel in the word-final clusters containing a consonant and a syllabic /l/ or /n/ due to the L1–L2 transfer. L1 Polish speakers are mostly unaware of the existence of syllabic consonants; hence, they use the Polish phonotactics and articulate a vocalic sound before a final sonorant which is deprived of its syllabicity. This phenomenon was examined among L1 Polish speakers, 1-year students of English studies, and the recording sessions were repeated a year later. Since, over that time, they were instructed with regard to phonetics and phonology but also the overall practical language learning, the results demonstrated the occurrence of the phenomenon of vowel insertion on different levels of advanced command of English. If the vowels were inserted, their quality and length were monitored and analysed. With regard to the English system, pronouncing vowel /ə/ before a syllabic consonant is possible, yet not usual. That is why another aim of this study is to examine to what extent the vowels articulated by the subjects differ from the standard pronunciation of non-final /ə/. The quality differences between the vowels articulated in the words ending with /l/ and /n/ were examined as well as the potential influence from the difference between /l/ and /n/ on the occurrence of vowel reduction. Even though Polish phonotactics permit numerous consonantal combinations in all word positions, it proved to be challenging for L1 Polish speakers to pronounce word-final consonantal clusters containing both syllabic sonorants. This result carries practical implications for the teaching methodology of English phonetics.

The role of microtubules in chick blastoderm expansion—a quantitative study using colchicine

Development ◽  
1975 ◽  
Vol 34 (1) ◽  
pp. 265-277
Author(s):  
J. R. Downie
Keyword(s):  
Cell Division ◽  
Cell Shape ◽  
Shape Change ◽  
Cell Sheet ◽  
Cell Movement ◽  
Vitelline Membrane ◽  
General Context ◽  
Chick Blastoderm ◽  
Cell Shape Change ◽  
Cytoplasmic Microtubules

Since their discovery, cytoplasmic microtubules have been much studied in the context of cell movement and cell shape change. Much of the work has used drugs, particularly colchicine and its relatives, which break down microtubules — the so-called anti-tubulins. Colchicine inhibits the orientated movements of many cell types in vitro, and disrupts cell shape change in several morphogenetic situations. The investigation reported here used chick blastoderm expansion in New culture in an attempt to quantify the colchicine effect on orientated cell movement. However, although colchicine could halt blastoderm expansion entirely, a simple interpretation was not possible. (1) Colchicine at concentrations capable of blocking mitosis, and of disrupting all or most of the cytoplasmic microtubules of the cells studied, inhibited blastoderm expansion, often resulting in an overall retraction of the cell sheet. (2) Though blastoderm expansion does normally involve considerable cell proliferation, the colchicine effect could not be ascribed to a block on cell division since aminopterin, which stops cell division without affecting microtubules, did not inhibit expansion. (3) Blastoderm expansion is effected by the locomotion of a specialized band of edge cells at the blastoderm periphery. These are the only cells normally attached to the vitelline membrane — the substrate for expansion. When most of the blastoderm was excised, leaving the band of edge cells, and the cultures then treated with colchicine, expansion occurred normally. The colchicine effect on blastoderm expansion could not therefore be ascribed to a direct effect on the edge cells. (4) An alternative site of action of the drug is the remaining cells of the blastoderm. These normally become progressively flatter as expansion proceeds. If flattening in these cells is even partially dependent on their cytoplasmic microtubules, disruption of these microtubules might result in the inherent contractility of the cells resisting and eventually halting edge cell migration. That cell shape in these cells is dependent on microtubules was demonstrated by treating flat blastoderm fragments with colchicine. On incubation, the area occupied by these fragments decreased by 25–30 % more than controls. The significance of these results in the general context of orientated cell movements and cell shape determination is discussed, with particular emphasis on the analogous system of Fundulus epiboly.

Distinction between Dolus and Culpa with reference to Arson in Zakon Sudnyj LJudem, the Vinodol Law and the Statute of Senj

De Jure ◽  
2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Daniel Haman ◽  
◽  
◽  
Keyword(s):  
Criminal Law ◽  
The Other ◽  
Legal Documents ◽  
The Third ◽  
Other Hand ◽  
Criminal Offenses ◽  
The World ◽  
The Difference ◽  
Different Levels ◽  
Better Than

The difference between intent (dolus) and negligence (culpa) was rarely emphasized in codified medieval laws and regulations. When compared to the legal statements related to intent, negligence was mentioned even more rarely. However, there are some laws that distinguished between the two concepts in terms of some specific crimes, such as arson. This paper draws attention to three medieval Slavic legal documents – the Zakon Sudnyj LJudem (ZSLJ), the Vinodol Law and the Statute of Senj. They are compared with reference to regulations regarding arson, with the focus being on arson as a crime committed intentionally or out of negligence. The ZSLJ as the oldest known Slavic law in the world shows some similarities with other medieval Slavic legal codes, especially in the field of criminal law, since most of the ZSLJ’s articles are related to criminal law. On the other hand, the Vinodol Law is the oldest preserved Croatian law and it is among the oldest Slavic codes in the world. It was written in 1288 in the Croatian Glagolitic script and in the Croatian Chakavian dialect. The third document – the Statute of Senj – regulated legal matters in the Croatian littoral town of Senj. It was written in 1388 – exactly a century after the Vinodol Law was proclaimed. When comparing the Vinodol Law and the Statute of Senj with the Zakon Sudnyj LJudem, there are clear differences and similarities, particularly in the field of criminal law. Within the framework of criminal offenses, the act of arson is important for making a distinction between intent and negligence. While the ZSLJ regulates different levels of guilt, the Vinodol Law makes no difference between dolus and culpa. On the other hand, the Statute of Senj strictly refers to negligence as a punishable crime. Even though the ZSLJ is almost half a millennium older than the Statute of Senj and around 400 years older than the Vinodol Law, this paper proves that the ZSLJ defines the guilt and the punishment for arson much better than the other two laws.

Fine structural observations of Desulfovibrio desulfuricans

1973 ◽  
Vol 19 (6) ◽  
pp. 753-756
Author(s):  
Terrence M. Hammill ◽  
Geno J. Germano
Keyword(s):  
Electron Microscopy ◽  
Cell Division ◽  
Cell Envelope ◽  
Desulfovibrio Desulfuricans ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Ultrathin Sections ◽  
Basal Apparatus ◽  
Specialized Region ◽  
Whole Mounts

Glutaraldehyde-fixed, platinum-carbon-shadowed whole mounts, and ultrathin sections of glutaraldehyde-OsO4-fixed cells of Desulfovibrio desulfuricans were observed by electron microscopy. The preparations demonstrated a typical Vibrio form with a single polar flagellum. The cell envelope and the formation of external blebs were shown to be similar to other gram-negative bacteria. The protoplast, apparently devoid of mesosomes or other membranous structures, was densely packed with ribosomes and contained a fibrous nucleoid. A specialized region near the flagellar end of the cell was commonly observed and termed the basal apparatus. Cell division appeared to be by constriction.

scholarly journals Newman’s Error Analysis Dalam Geometri Ruang Ditinjau dari Kecerdasan Visual Siswa SMA

2019 ◽  
Vol 3 (2) ◽  
pp. 447-461
Author(s):  
Ardina Fahriyanti Maharani ◽  
Erlina Prihatnani
Keyword(s):  
High School ◽  
Error Analysis ◽  
Transformation Process ◽  
Intelligence Test ◽  
Solid Geometry ◽  
Visual Intelligence ◽  
The Difference ◽  
Point Distance ◽  
Different Levels ◽  
Geometry Test

In solid geometry needed the ability to visualize space that cannot be seen so differences in visual intelligence can trigger errors in solving geometry problems. Therefore, this study aimed to determine the types of errors made by students and the factors that cause errors in working on geometry problems based on Newman's Error Analysis (NEA) in terms of visual intelligence. This research chose the subjects of class XII high school who had studied the material distance points to lines in space with different levels of visual intelligence, namely high and low. The process of collecting data used visual intelligence test questions, geometry test questions, and interviews. The results of this study indicated that the level of students' visual intelligence affects the difference in location and the factor that causes errors in the stage of transformation especially on determining the distance in dimension 3. Students with high visual intelligence made mistakes at the stage of transformation and encoding that caused by the students’ carelessness. However, students with low visual intelligence made mistakes at the stage of comprehension, transformation, process skills, and encoding due to the concept of the point distance to the line and the concept of the Pythagorean theorem.

scholarly journals Investigation of monthly variations in the efficiencies of photovoltaics due to sunrise and sunset times

2020 ◽  
Vol 18 (1) ◽  
pp. 310
Author(s):  
Armstrong O. Njok ◽  
Julie C. Ogbulezie ◽  
Manoj Kumar Panjwani ◽  
Raja Masood Larik
Keyword(s):  
Relative Humidity ◽  
Solar Energy ◽  
Electrical Energy ◽  
Time Of Day ◽  
Peak Efficiency ◽  
Pv Module ◽  
Average Relative Humidity ◽  
The Difference ◽  
Monthly Variations ◽  
Different Levels

<p>The effect of time of day and month on the efficient conversion of solar energy to electrical energy using a polycrystalline (PV) module in calabar was studied. A KT-908 precision digital hygrometer and thermometer, and a M890C+ digital multimeter were used in the process. Results obtained shows that photovoltaic produce different levels of peak efficiencies at different times of the day for different months due to the difference in sunrise and sunset times for the months. The results also indicated that photovoltaics will be more efficient in months with low average relative humidity couple with low panel temperature. A peak efficiency of 77% at 12:30 in the month of April was observed before dropping to 73% at 12:00 in the month of May, indicating that there might be further drop in efficiency as we proceed further into the year. Results also show that photovoltaics are more efficient before noon in the month of May than in April while the reverse will be observed in the afternoon. </p>

scholarly journals STRATEGI GURU DALAM MENGOPTIMALKAN KECERDASAN MAJEMUK DI SEKOLAH DASAR

2021 ◽  
Vol 7 (1) ◽  
pp. 15
Author(s):  
Robiatul Munajah ◽  
Asep Supena
Keyword(s):  
Elementary Schools ◽  
Literature Review ◽  
Multiple Intelligences ◽  
Learning Process ◽  
Know How ◽  
Way Of Thinking ◽  
The Difference ◽  
The Given ◽  
Different Levels

The success of students in learning does not only depend on their own abilities. Several factors that can give effect need to be optimized. The teacher's strategy is very meaningful to optimize students' multiple intelligences according to the indicators that each student has. Every child in this world has various intelligences in different levels and indicators. This shows that all children, by nature, are intelligent. The difference lies in the level and indicators of intelligence. These differences are determined by various factors. One of them is the stimulation given when children learn in the learning process carried out by the teacher. The difference in intelligence among students demands a fair and existential way of thinking of educators. This research is a literature review to see more specifically the teacher's strategy in optimizing multiple intelligences in elementary schools based on research reference sources and books. Good educators are able to detect children's intelligence by observing the behavior, tendencies, interests, ways and qualities of children when reacting to the given stimulus. All indicators of intelligence can be recognized by educators to then make a profile of intelligence. Therefore, every teacher should know how to develop the intelligence of their students, by identifying each indicator of children's intelligence and realizing the importance of developing all the intelligences of their students. 

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