scholarly journals Carriage of ESBL and Amp-C -b -lactamase among Escherichia coli strains isolated from dogs in kennels Dak Lak province

2021 ◽  
Vol 5 (2) ◽  
pp. 1198-1207
Author(s):  
Kien Chi Le ◽  
Cuong Quoc Vo ◽  
Xuan Thanh Tran ◽  
Hung Manh Dang ◽  
Huyen Ngoc My Nguyen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Viet Nam ◽  
Beta Lactamase ◽  
Sensitivity Testing ◽  
Beta Lactamases ◽  
E Coli ◽  
Antimicrobial Sensitivity ◽  
Extended Spectrum ◽  
Genes Encoding

The global prevalence of antimicrobial resistance and Extended-Spectrum and AmpC Beta- Lactamases is continuously widespread among Escherichia coli during recent years, especially in Viet Nam. In Viet Nam, there have been researches on ESBL and AmpC-carrying E. coli inhabiting animal and human. However, studies of antimicrobial resistance in E. coli residing in pets, especially dogs are unavailable. The aim of the study was to investigate the antimicrobial sensitivity testing (AST), the resistance to 3rd cephalosporin and penicillin, also to assess the molecular detection of ESBL and Amp-C-beta -lactamase in E. coli isolates inhabiting the digestive tract of dogs at kennels Dak Lak. By using double disk synergy test (DDST), and ceftazidime-imipenem antagonism test (CIAT) to detect phenotypic characteristic of E. coli strains producing extended-spectrum beta- lactamases (ESBLs) and plasmid-mediated Amp-C-beta -lactamase, and by using multiplex polymerase chain reaction (multiplex PCR) to confirm the presence of ESBL genes (class A): blaCTX-M(1;2;8;9;25), bla TEM, bla SHV , bla OXA and genes encoding AmpC-type beta lactamase (class C): bla MOX-1;2 , bla CMY- (1;2-7;8-11) , blaLAT-(1;4) ,bla DHA-(1;2), bla ACC, bla FOX-(1-5B) ,bla MIR-1 ,bla ACT-1. From of three hundred twelve bacteial strains isolated from sixty-four rectal swabs two hundred sixty-nine E. Coli, isolates accounting for 86%, were identified and isolated, forty-four (16%) and twelve (4%) E. coli isolates encoding with ESBL and Amp-C-beta -lactamases. From molecular diagnosis with regard to phenotype, production of ESBL was shown in thirty-nine (15%) E. coli isolates and Amp-C enzymes in eight (3%) E. coli isolates. The high percentage of E. coli exhibiting antibiotic resistance revealed the accelerated overuse of antibiotics. Result of this study will contribute to the monitoring of epidemiologic resistance.

scholarly journals Extended Spectrum Beta-Lactamase Escherichia coli in River Waters Collected from Two Cities in Ghana, 2018–2020

2021 ◽  
Vol 6 (2) ◽  
pp. 105
Author(s):  
Regina Ama Banu ◽  
Jorge Matheu Alvarez ◽  
Anthony J. Reid ◽  
Wendemagegn Enbiale ◽  
Appiah-Korang Labi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
River Waters ◽  
E Coli ◽  
Extended Spectrum ◽  
Two Cities ◽  
Animal Wastewater ◽  
Sources Of Contamination

Infections by Extended-Spectrum Beta-Lactamase producing Escherichia coli (ESBL-Ec) are on the increase in Ghana, but the level of environmental contamination with this organism, which may contribute to growing Antimicrobial Resistance (AMR), is unknown. Using the WHO OneHealth Tricycle Protocol, we investigated the contamination of E. coli (Ec) and ESBL-Ec in two rivers in Ghana (Odaw in Accra and Okurudu in Kasoa) that receive effluents from human and animal wastewater hotspots over a 12-month period. Concentrations of Ec, ESBL-Ec and percent ESBL-Ec/Ec were determined per 100 mL sample. Of 96 samples, 94 (98%) were positive for ESBL-Ec. concentrations per 100 mL (MCs100) of ESBL-Ec and %ESBL-Ec from both rivers were 4.2 × 104 (IQR, 3.1 × 103–2.3 × 105) and 2.79 (IQR, 0.96–6.03), respectively. MCs100 were significantly lower in upstream waters: 1.8 × 104 (IQR, 9.0 × 103–3.9 × 104) as compared to downstream waters: 1.9 × 106 (IQR, 3.7 × 105–5.4 × 106). Both human and animal wastewater effluents contributed to the increased contamination downstream. This study revealed high levels of ESBL-Ec in rivers flowing through two cities in Ghana. There is a need to manage the sources of contamination as they may contribute to the acquisition and spread of ESBL-Ec in humans and animals, thereby contributing to AMR.

scholarly journals Incidence and Antimicrobial Susceptibility of Escherichia coli and Klebsiella pneumoniae with Extended-Spectrum β-Lactamases in Community- and Hospital-Associated Intra-Abdominal Infections in Europe: Results of the 2008 Study for Monitoring Antimicrobial Resistance Trends (SMART)

2010 ◽  
Vol 54 (7) ◽  
pp. 3043-3046 ◽  
Author(s):  
Stephen P. Hawser ◽  
Samuel K. Bouchillon ◽  
Daryl J. Hoban ◽  
Robert E. Badal ◽  
Rafael Cantón ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Klebsiella Pneumoniae ◽  
Antimicrobial Susceptibility ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Abdominal Infections

ABSTRACT From 2002 to 2008, there was a significant increase in extended-spectrum beta-lactamase (ESBL)-positive Escherichia coli isolates in European intra-abdominal infections, from 4.3% in 2002 to 11.8% in 2008 (P < 0.001), but not for ESBL-positive Klebsiella pneumoniae isolates (16.4% to 17.9% [P > 0.05]). Hospital-associated isolates were more common than community-associated isolates, at 14.0% versus 6.5%, respectively, for E. coli (P < 0.001) and 20.9% versus 5.3%, respectively, for K. pneumoniae (P < 0.01). Carbapenems were consistently the most active drugs tested.

scholarly journals Occurrence of extended-spectrum beta-lactamaseand AmpC-type cephalosporinase-producing Escherichia coli in mastitic cow’s milk

2021 ◽  
Vol 77 (06) ◽  
pp. 291-294
Author(s):  
HANNA RÓŻAŃSKA ◽  
MARIA KUBAJKA ◽  
MARCIN WEINER
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Cow’S Milk ◽  
Macconkey Agar ◽  
E Coli ◽  
Milk Samples ◽  
Microdilution Method ◽  
Extended Spectrum ◽  
Genes Encoding ◽  
Esbl Producers

The aim of the study was to evaluate the occurrence of Escherichia coli producing extended-spectrum betalactamases (ESBL and/or AmpC) in the milk of cows with mastitis. A total 2,500 milk samples from mastitic cows were tested in 2014-2018. The investigations included the culture of bacteria on MacConkey agar with cefotaxime, identification with the API Rapid 32 E test, synergy disc test D68C, assessment of antimicrobial resistance by the microdilution method and confirmation of the occurrence of genes encoding ESBL and AmpC. Out of 133 isolates identified as E. coli, 87 were recognized as ESBL producers and 46 as chromosomally encoded cephalosporinase AmpC producers. The blaTEM was predominant in the ESBL producers. All 46 AmpC-positive strains had the blaCMY gene. The results confirmed the occurrence of extended-spectrum betalactamase- producing E. coli in inflammatory secretions from mastitic

scholarly journals Phenotypic Characterization of Multidrug-resistant Escherichia Coli with Special Reference to Extended-spectrum-beta-lactamases and Metallo-beta-lactamases in a Tertiary Care Center

2015 ◽  
Vol 53 (198) ◽  
pp. 83-88 ◽  
Author(s):  
Basudha Shrestha ◽  
Shovita Shrestha ◽  
Shyam Kumar Mishra ◽  
Hari Prasad Kattel ◽  
Tatsuya Tada ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
Resistance Mechanism ◽  
Multidrug Resistant ◽  
Confirmatory Test ◽  
Beta Lactamase ◽  
Beta Lactamases ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum

Introduction: The increasing reports on extended-spectrum-beta-lactamase and metallo-betalactamase producing Escherichia coli have addressed a potential threat to global health since it is found to be highly resistance to most of the currently available antibiotics including carbapenems. The present study was aimed to determine the antibiogram of extended-spectrum-beta-lactamase and metallo-beta-lactamase producing MDR E. coli isolates from various clinical samples. Methods: This was a cross-sectional study conducted over a period of seven months (December 2013 to July 2014) at bacteriology laboratory of Tribhuvan University Teaching Hospital. A total of 250 clinical specimens (urine, pus, sputum, blood, body fluid, bile, tissue and central venous pressure line tip) were processed from inpatients, with multidrug-resistant Escherichia coli infections. Standard microbiological techniques were used for isolation and identification of the isolates. The presence of extended-spectrum-beta-lactamase was detected by phenotypic confirmatory test recommended by Clinical and Laboratory Standards Institute and imipenem (IMP) /EDTA combined disc method was performed to detect metallo-beta-lactamase mediated resistance mechanism. Results: We found high level of beta lactamase mediated resistance mechanism as part of multidrug resistance. Among 250 MDR isolates, 60% isolates were extended-spectrum-beta-lactamase producers and 17.2% isolates were metallo-beta-lactamase producers. Co-existence of extended-spectrum-betalactamase and metallo-beta-lactamase identified in 6.8% isolates. Conclusions: Beta-lactamase mediated resistance mechanisms are accounting very high in the multidrug resistant isolates of E. coli. Therefore, early detection of beta lactamase mediated resistant strains and their current antibiotic susceptibility pattern is necessary to avoid treatment failure and prevent the spread of MDR.  Keywords: e. coli; extended-spectrum-β-lactamase; metallo-β-lactamase; multidrug-resistance.

scholarly journals Antimicrobial Resistance Profile and Extended Spectrum Beta-Lactamase Resistance Genes in Escherichia coli from Poultry Droppings in Nasarawa, Nigeria

2021 ◽  
pp. 47-56
Author(s):  
S. C. Tama ◽  
Y. B. Ngwai ◽  
G. R. I. Pennap ◽  
I. H. Nkene ◽  
R. H. Abimiku ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Resistance Genes ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Antimicrobial Resistance Profile ◽  
Poultry Farms ◽  
Poultry Droppings

Aims: This study investigated the antimicrobial resistance profile and extended spectrum beta-lactamase resistance genes of Escherichia coli isolated from droppings of from selected poultry farms in Nasarawa, Nigeria. Study Design: Investigative Place and Duration of Study: Department of Microbiology, Nasarawa State University, Keffi, between November 2019 and February 2020. Methodology: A total of 90 samples from poultry droppings were collected from selected farms. Escherichia coli was isolated from the samples using standard cultural and microbiological methods. Antibiotic susceptibility testing and minimum inhibitory concentrations were evaluated as described by the Clinical and Laboratory Standards Institute (CLSI). The detection of extended-spectrum beta-lactamase (ESBL) production in E. coli isolates was carried out using double disc synergy test.  In addition, molecular detection of ESBL genes was carried out using Polymerase Chain Reaction (PCR) method. Results: The prevalence of E. coli was 100%. Antibiotic resistances of E. coli were recorded as follows: streptomycin (S: 94.4%), sulphamethoxazole / trimethoprim (SXT: 90.0%), ampicillin (AMP: 88.9%), gentamicin (CN: 68.9%), amoxicillin/clavulanic acid (AMC: 55.6%), ciprofloxacin (CIP: 41.1%), cefoxitin (FOX: 35.6%), ceftazidime (CAZ: 34.4%), cefotaxime (CTX: 22.2%), and imipenems (IPM: 17.8%). The most common antibiotic resistant resistance phenotype was AMP-CTX-CAZ-CIP-CN (11.1%). Multiple antibiotic resistance (MAR) was observed in 97.7% (88/90) of the isolates, with the common MAR index being 0.5 (33.3%). Twenty five of the thirty beta-lactam resistant isolates (83.3%) were confirmed ESBL producers. The 25 ESBL positive isolates carried bla genes as follows: blaTEM (11/25, 44.0%) and blaCTX-M (18/25, 72.0%). blaSHV was not found in any isolate. Conclusion: E. coli isolated from the droppings of selected poultry farms in Nasarawa were less resistant to imipenem, cefotaxime, ceftazidime and cefoxitin in the study location. This implies that the antibiotics are useful in the treatment of infection caused by E. coli. Also, ESBL-positive E. coli isolates harbored ESBL genes, with blaCTX-M as the most common.

scholarly journals Antimicrobial Resistance Profiling and Molecular Epidemiological Analysis of Extended Spectrum β-Lactamases Produced by Extraintestinal Invasive Escherichia coli Isolates From Ethiopia: The Presence of International High-Risk Clones ST131 and ST410 Revealed

2021 ◽  
Vol 12 ◽  
Author(s):  
Abebe Aseffa Negeri ◽  
Hassen Mamo ◽  
Jyoti M. Gurung ◽  
A. K. M. Firoj Mahmud ◽  
Maria Fällman ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Multidrug Resistance ◽  
High Risk ◽  
Antimicrobial Resistance ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Bacterial Typing ◽  
E Coli ◽  
Extended Spectrum ◽  
Genes Encoding

The treatment of invasive Escherichia coli infections is a challenge because of the emergence and rapid spread of multidrug resistant strains. Particular problems are those strains that produce extended spectrum β-lactamases (ESBL’s). Although the global characterization of these enzymes is advanced, knowledge of their molecular basis among clinical E. coli isolates in Ethiopia is extremely limited. This study intends to address this knowledge gap. The study combines antimicrobial resistance profiling and molecular epidemiology of ESBL genes among 204 E. coli clinical isolates collected from patient urine, blood, and pus at four geographically distinct health facilities in Ethiopia. All isolates exhibited multidrug resistance, with extensive resistance to ampicillin and first to fourth line generation cephalosporins and sulfamethoxazole-trimethoprim and ciprofloxacin. Extended spectrum β-lactamase genes were detected in 189 strains, and all but one were positive for CTX-Ms β-lactamases. Genes encoding for the group-1 CTX-Ms enzymes were most prolific, and CTX-M-15 was the most common ESBL identified. Group-9 CTX-Ms including CTX-M-14 and CTX-27 were detected only in 12 isolates and SHV ESBL types were identified in just 8 isolates. Bacterial typing revealed a high amount of strains associated with the B2 phylogenetic group. Crucially, the international high risk clones ST131 and ST410 were among the sequence types identified. This first time study revealed a high prevalence of CTX-M type ESBL’s circulating among E. coli clinical isolates in Ethiopia. Critically, they are associated with multidrug resistance phenotypes and high-risk clones first characterized in other parts of the world.

scholarly journals Antimicrobial resistance characteristics of Extended Spectrum Beta Lactamase (ESBL)- producing Escherichia coli from dairy farms in the Sleman district of Yogyakarta province, Indonesia

2021 ◽  
Vol 19 (3) ◽  
pp. 525-535
Author(s):  
Kusnul Yuli Maulana ◽  
◽  
Duangporn Pichpol ◽  
Nur Rohmi Farhani ◽  
Dyah Ayu Widiasih ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Antimicrobial Resistance ◽  
Dairy Farms ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum ◽  
Microbiological Methods ◽  
Water Feed

Extended Spectrum Beta Lactamase (ESBL)-producing Escherichia coli (E. coli) infections are a global health challenge resulting from human contact with infected animals and contaminated farm environments. This study aims to identify antimicrobial resistance patterns of ESBL-producing E. coli isolated from dairy farms in the Sleman District of Yogyakarta Province, Indonesia. Ninety-three dairy farms with a history of antibiotic use in the previous 6 months were identified. Samples were collected from 6 different sources (feces, milk, wastewater, animal drinking water, feed and rinses of workers’ hands) on each farm during August through November 2020. These samples were cultured with conventional microbiological methods for the isolation of ESBL-producing E. coli. ESBL-producing E. coli was identified in one or more of the sources in 54% (50/93) of the dairy farms sampled. Fecal samples were the most commonly positive (25%) while wastewater, animal drinking water feed, milk and hand rinses were positive at 16%, 10%, 5%, 4% and 3% respectively. Colonies from each positive sample were screened for antibiotic susceptibility test using the Vitek-2 system. Resistance to trimethoprim/sulfamethoxazole, tetracycline and gentamicin were found in 74%, 63% and 48% of the isolates, respectively. Multidrug resistant (MDR) was identified in 50% (63/127) of the isolates. In conclusion, ESBL-producing E. coli appears widespread in dairy farms using antibiotics and antimicrobial resistance among these bacteria is common in this study area. Further study of the risk of human transmission from contaminated cattle and their environments could benefit the national antimicrobial resistance strategic plans.

scholarly journals Molecular Epidemiology of Escherichia coli Sequence Type 131 and Its H30 and H30-Rx Subclones among Extended-Spectrum-β-Lactamase-Positive and -Negative E. coli Clinical Isolates from the Chicago Region, 2007 to 2010

2013 ◽  
Vol 57 (12) ◽  
pp. 6385-6388 ◽  
Author(s):  
Ritu Banerjee ◽  
Ari Robicsek ◽  
Michael A. Kuskowski ◽  
Stephen Porter ◽  
Brian D. Johnston ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Virulence Genes ◽  
Sequence Type ◽  
Beta Lactamase ◽  
Esbl Production ◽  
Content Type ◽  
Extended Spectrum Beta Lactamase ◽  
E Coli ◽  
Extended Spectrum

ABSTRACTWe assessedEscherichia coliST131 and its H30 and H30-Rx subclones for virulence genes, antimicrobial resistance, and extended-spectrum beta-lactamase (ESBL) type. Although both subclones were associated with ESBL production, H30-Rx isolates had higher resistance scores and were associated specifically with CTX-M-15. Three virulence genes (iha,sat, andiutA) were more prevalent among H30 than non-H30 ST131 isolates. Thus, the H30 and H30-Rx subclones are more antimicrobial resistant and have virulence profiles that are distinct from those of non-H30 ST131 isolates.

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