scholarly journals Antifungal Activity of Essential Oils of Leaves, Rhizomes Oils and Fraction Wild Ginger Elettariopsis Slahmong Ck Lim Inhibit The Colony Growth of Sclerotium Rolfsii

2021 ◽  
Vol 5 (1) ◽  
pp. 28-37
Author(s):  
Nurmansyah Nurmansyah ◽  
Herwita Idris ◽  
Nasril Nasir

This study aims to see the effectiveness  of essential oils of leaves, rhizomes and fraction of wild ginger Ellettariopsis slahmong CK Lim against the pathogenic fungus Sclerotium rolfsii which causes rot disease of the stem base of peanut plants an in vitro.The study consisted of two sub activities: (a) inhibited of colony diameter using Patato Dextrosa Agar (PDA) medium and (b) inhibited of colony biomass using Potato dextrose Broth (PDB) medium, the treatments tested were leaf essential oil and rhizome of wild ginger and fractions A1, B2, C3 and D4, with concentration levels (0, 100, 250 and 500 ppm). Experiments (a) and (b) were arranged in the form of a Completely Randomized Design (CRD) in factorial each with 4 replications. The results showed that the leaf essential oil and rhizome of wild ginger and its fractions had the potential to be used as a vegetable fungicide. The A1 fraction has the best antifungal effectiveness compared to the B2 fraction, leaf oil, rhizomes and other fractions, with the highest inhibition of diameter and biomass of S rolfsii colony the 49.47% and 51.46%. Essential oils of leaves and rhizome oil are not statistically significantly different, but in numerically leaf oil are better than rhizome oil. The C3 fraction showed the lowest colony diameter inhibition and biomass of 34.70% and 36.95%. The best concentration level in inhibition the growth of S rolfsii mushroom is 500 ppm, with inhibition of the diameter and biomass of the colony by 81.74% and 84.25%.

2009 ◽  
Vol 4 (6) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Claudia Giuliani ◽  
Roberto Maria Pellegrino ◽  
Bruno Tirillini ◽  
Laura Maleci Bini

The essential oils from both leaves (L) and flowers (F) of Stachys germanica subsp. salviifolia (Ten.) Gams (Labiatae/Lamiaceae) from Italy were analyzed by GC-MS for the first time. The distribution, morphology and histochemistry of the different types of glandular trichomes present on the epidermal surface were also determined. Twenty-nine constituents, representing 89.4% of the total leaf oil, and forty-one compounds, forming 97.8% of the total flower oil, were identified. Germacrene D (39.4%), phytol (10.2%), β-bourbonene (3.5%) and β-ylangene (3.3%) were recognized as the main constituents of the leaf essential oil, while limonene (24.1%), β-pinene (18.7%), germacrene D (12.8%) and ( E)-nerolidol (6.6%) were the main compounds of the flower essential oil.


2013 ◽  
Vol 8 (10) ◽  
pp. 1934578X1300801 ◽  
Author(s):  
Prajwal Paudel ◽  
Prabodh Satyal ◽  
Noura S. Dosoky ◽  
Samjhana Maharjan ◽  
William N. Setzer

The essential oils from the leaves of Juglans regia, collected from Kathmandu, Nepal, and Juglans nigra from Huntsville, Alabama, USA, were obtained by hydrodistillation and analyzed by GC-MS. A total of 69 compounds were identified in the J. regia oil, accounting for 99.0% of the eluting components of the oil while three different samples of J. nigra were analyzed identifying a total of 99.3%, 99.4% and 99.7% of the oils. The leaf essential oil of J. regia was dominated by the aromatic compounds eugenol (27.5%) and methyl salicylate (16.2%), and the sesquiterpenes germacrene D (21.4%) and ( E)-β-farnesene (8.2%). The essential oils from three different samples of J. nigra contained ( E)-caryophyllene (17.3%–20.4%) and germacrene D (7.1%–22.5%) with smaller amounts of juglone (1.0%–8.8%), α-hydrojuglone (1.0–9.5%), and δ-cadinene (3.8%–8.7%). J. regia leaf oil, devoid of juglone, showed allelopathic activity, while J. nigra leaf oil was less phytotoxic. The presence of eugenol and methyl salicylate in the leaves of J. regia are consistent with the traditional uses of this plant to treat toothache, rheumatism, and fungal infections.


2019 ◽  
Vol 7 (2) ◽  
pp. 163
Author(s):  
Nasrun Ibrahim ◽  
Jalaluddin Jalaluddin ◽  
Nurul Rahmah

Indonesia has natural resources that are rich in essential oils. One potential natural resource is lime which can be used as flavor in food. The extraction of lime leaf essential oil using volatile solvent extraction method. The aim of the study was to determine the effect of extraction time on yield, refractive index, and density of essential oils produced. Extraction of lime leaf oil with n-hexane solvent using soxhlet extractors. The old lime leaves that have been cleaned, cut into small pieces and wrapped in filter paper and put in soxhlet. 200 ml n-hexane solvent is inserted in an extractor round bottom flask equipped with coolant. Extraction is carried out at a certain temperature and time depending on the type of solvent used, until the solvent color is returned as before. Then the filtrate was distilled to be purified, so that lime leaf oil was obtained separately from the solvent. Essential oils are then tested for yield, refractive index, and density contained in essential oils. The results of the study were extraction of lime leaves with n-hexane solvent, the highest yield obtained at a sample weightof 200 grams with an extraction time of 180 minutes which was 3.11%. The highest oil refractive index was also obtained at a sample weight of 200 grams with an extraction time of 180 minutes which was 1.47, while the density of essential oil from lime leaves was 0.79gr/ml.Keywords: essential oil, lime leaf, extraction, n-heksana


2010 ◽  
Vol 5 (6) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Raphaël Bikanga ◽  
Thomas Makani ◽  
Huguette Agnaniet ◽  
Louis Clément Obame ◽  
Fatouma Mohamed Abdoul-Latif ◽  
...  

Water-distilled essential oils from leaves and bark of Santiria trimera (Oliv.) Aubrév. (Burseraceae) collected in Gabon were analyzed using capillary GC-FID and GC-MS. The leaf essential oil, which is described for the first time, was dominated by sesquiterpenes (76.5%), among which α-humulene (34.6%) and β-caryophyllene (14.9%) were the major components. The bark essential oil was almost exclusively monoterpenic, with α-pinene (51.5%) and α-terpineol (16.8%) as main constituents. The antimicrobial activities of both essential oils were tested against eight Gram-positive and three Gram-negative bacteria by using agar disc diffusion and broth microdilution methods; anticandidal effect was also tested on different strains of Candida albica. The Gram-negative bacteria were the less sensitive to the leaf essential oil, which was effective against Bacillus cereus and Enterococcus faecalis. The bark essential oil was more active and, in particular, exhibited significant antimicrobial activity against Proteus mirabilis, which was resistant to the leaf oil. A weak anticandidal effect of both essential oils was observed.


2020 ◽  
Vol 1 (1) ◽  
pp. 67
Author(s):  
Munira Munira

Background: Some plants that are included in the genus Citrus are one of the plants producing essential oils which is a natural substance that is known to have an antibacterial effect.Objectives: The study aims to determine the ability of essential oils from the leaves of several types of citrus (Citrus) in inhibiting the growth of Escherichia coli.Methods: An experimental study using a complete randomized design consisting of 4 treatments and four replications each. The treatments consisted of distilled water (control), lime leaf essential oil, kaffir lime essential oil and lime leaf essential oil. Microbiology test uses agar diffusion method.Results: Anova test showed that essential oils from some lime can inhibit the growth of Escherichia coli (p = 0.000). Duncan's further test results showed that the average diameter of the inhibition zone formed showed that the average diameter of the largest inhibition zone was formed in the treatment which was given lime orange essential oil (16.55 mm) and was not significantly different (p <0.05) with orange essential oil kaffir (15.40 mm) and lime essential oil (14.97 mm). Based on the Duncan test results showed that there is no difference in the average diameter of inhibitory zones between essential oils of lime, lime and lime, this can be caused because the three lime are classified in the same genus, namely the genus citrus.Conclusion: Essential oils from some lime can inhibit the growth of Escherichia coli. The average diameter of the inhibition zone that formed showed the greatest inhibition formed in the treatment which was given lime essential oil.


2021 ◽  
Author(s):  
Huanling Xing ◽  
Yingqi Hu ◽  
Liupeng Yang ◽  
Jianhao Lin ◽  
Haiyi Bai ◽  
...  

Abstract The high toxicity, long-lasting residues, and resistance to chemical pesticides and baits used in the control of red imported fire ants could possibly harm human health and ecological balance. Natural compounds would be an excellent source of pesticides because of their good repellency and insecticidal properties. In this study, the fumigation method was used to study the insecticidal effects of essential oils from the bark and leaves of Cinnamomum loureirii Nees on red imported fire ants at different concentrations and fumigation time. The fumigation time with C. loureirii essential oils was positively correlated with the knockdown ability and mortality in red imported fire ants and was negatively correlated with grasping ability. The insecticidal activity of the essential oil extracted from C. loureirii leaves was significantly higher than that of the essential oils extracted from bark at effective concentrations. Cinnamyl acetate, an abundant component in leaf essential oil, plays an important role in improving the insecticidal activity of trans-cinnamaldehyde. This study provides a theoretical basis for the development and utilization of cinnamon leaf oil as a new environmentally friendly insecticide resource for the control of red imported fire ants.


2010 ◽  
Vol 5 (7) ◽  
pp. 1934578X1000500
Author(s):  
Chen-Lung Ho ◽  
Pei-Chun Liao ◽  
Kuang-Ping Hsu ◽  
Eugene I-Chen Wang ◽  
Wei-Chih Dong ◽  
...  

The hydrodistillated leaf essential oil of Machilus pseudolongifolia was analyzed to determine its composition and yield. Seventy compounds were identified, the main components being β-eudesmol (26.8%), α-cadinol (20.8%), viridiflorene (8.9%), α-caryophyllene (5.3%), globulol (4.6%) and β-caryophyllene (4.2%). Oxygenated sesquiterpenes (60.1%) and sesquiterpene hydrocarbons (31.4%) were the predominant groups of compounds. The leaf oil exhibited excellent antimicrobial and anti-wood-decay fungal activities.


2019 ◽  
Vol 5 (04) ◽  
pp. 293-296 ◽  
Author(s):  
Suresh Kumar Sharma ◽  
Shweta Singh ◽  
S. K. Tewari

The essential oils of Curcuma longa L. (Zingiberaceae) were extracted from senescenced leaves by hydrodistillation and analyzed by GC/MS. Leaf essential oils of turmeric were found to be rich in monoterpenes. The GCMS analysis of leaf essential oil of 29 accessions showed presence of total 41 compounds mainly α-phellandrene, p-cymene, α-terpinolene and p-cymen-8-ol as the four major compounds. Based on identification of three major compounds from each oil, 13 compounds viz. β-Myrcene, α-Phellandrene, α-Terpinene, Limonene, 1,8-Cineole, p-cymene, τ-terpenine, α-Terpinolene, 1,3,8-p-Menthatriene, p-menth-1-en-4-ol, Borneol, p-menth-1-en-8-ol and p-cymen-8-ol were found to be the major constituents from each accession. The number of compounds in accessions varied from five in NBH-20 to 22 in Prabha. Out of 41 compounds, δ-Elemene was reported only in R. Sonia while β-Bisabolene was detected in Roma and P. Peetabh only. Based on the major compound(s) of turmeric leaf oil, industrial applications can be explored.


Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1303 ◽  
Author(s):  
Do N. Dai ◽  
Nguyen T. Chung ◽  
Le T. Huong ◽  
Nguyen H. Hung ◽  
Dao T.M. Chau ◽  
...  

Members of the genus Cinnamomum (Lauraceae) have aromatic volatiles in their leaves and bark and some species are commercially important herbs and spices. In this work, the essential oils from five species of Cinnamomum (C. damhaensis, C. longipetiolatum, C. ovatum, C. polyadelphum and C. tonkinense) growing wild in north central Vietnam were obtained by hydrodistillation, analyzed by gas chromatography and screened for antimicrobial and mosquito larvicidal activity. The leaf essential oil of C. tonkinense, rich in β-phellandrene (23.1%) and linalool (32.2%), showed excellent antimicrobial activity (MIC of 32 μg/mL against Enterococcus faecalis and Candida albicans) and larvicidal activity (24 h LC50 of 17.4 μg/mL on Aedes aegypti and 14.1 μg/mL against Culex quinquefasciatus). Cinnamomum polyadelphum leaf essential oil also showed notable antimicrobial activity against Gram-positive bacteria and mosquito larvicidal activity, attributable to relatively high concentrations of neral (11.7%) and geranial (16.6%). Thus, members of the genus Cinnamomum from Vietnam have shown promise as antimicrobial agents and as potential vector control agents for mosquitoes.


2009 ◽  
Vol 1 (2) ◽  
pp. 387-392 ◽  
Author(s):  
M. N. I. Bhuiyan ◽  
J. Begum ◽  
P. K. Sardar ◽  
M. S. Rahman

The chemical constituents of leaf and peel essential oil of Citrus medica L. were analysed by gas chromatography mass spectroscopy (GC-MS). Nineteen components accounting for 99.9% of the oil were identified in leaf oil. The major constituents are erucylamide (28.43%), limonene (18.36%) and citral (12.95%). The peel oil contains forty three components accounting for 99.8% of the total oil and the major components are isolimonene (39.37%), citral (23.12%) and limonene (21.78%). Keywords: Citrus medica; Essential oils; GC-MS; Erucylamide; Isolimonene. © 2009 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.DOI: 10.3329/jsr.v1i2.1760   


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