scholarly journals Potensi antimikroba minyak atsiri daun jeruk (Citrus)

2020 ◽  
Vol 1 (1) ◽  
pp. 67
Author(s):  
Munira Munira
Keyword(s):  
Escherichia Coli ◽  
Essential Oil ◽  
Essential Oils ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Test Results ◽  
Water Control ◽  
Randomized Design ◽  
Leaf Essential Oil ◽  
Orange Essential Oil

Background: Some plants that are included in the genus Citrus are one of the plants producing essential oils which is a natural substance that is known to have an antibacterial effect.Objectives: The study aims to determine the ability of essential oils from the leaves of several types of citrus (Citrus) in inhibiting the growth of Escherichia coli.Methods: An experimental study using a complete randomized design consisting of 4 treatments and four replications each. The treatments consisted of distilled water (control), lime leaf essential oil, kaffir lime essential oil and lime leaf essential oil. Microbiology test uses agar diffusion method.Results: Anova test showed that essential oils from some lime can inhibit the growth of Escherichia coli (p = 0.000). Duncan's further test results showed that the average diameter of the inhibition zone formed showed that the average diameter of the largest inhibition zone was formed in the treatment which was given lime orange essential oil (16.55 mm) and was not significantly different (p <0.05) with orange essential oil kaffir (15.40 mm) and lime essential oil (14.97 mm). Based on the Duncan test results showed that there is no difference in the average diameter of inhibitory zones between essential oils of lime, lime and lime, this can be caused because the three lime are classified in the same genus, namely the genus citrus.Conclusion: Essential oils from some lime can inhibit the growth of Escherichia coli. The average diameter of the inhibition zone that formed showed the greatest inhibition formed in the treatment which was given lime essential oil.

scholarly journals Antifungal Activity of Essential Oils of Leaves, Rhizomes Oils and Fraction Wild Ginger Elettariopsis Slahmong Ck Lim Inhibit The Colony Growth of Sclerotium Rolfsii

2021 ◽  
Vol 5 (1) ◽  
pp. 28-37
Author(s):  
Nurmansyah Nurmansyah ◽  
Herwita Idris ◽  
Nasril Nasir
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Pathogenic Fungus ◽  
Sclerotium Rolfsii ◽  
Colony Growth ◽  
Potato Dextrose Broth ◽  
Colony Diameter ◽  
Randomized Design ◽  
Leaf Oil ◽  
Leaf Essential Oil

This study aims to see the effectiveness  of essential oils of leaves, rhizomes and fraction of wild ginger Ellettariopsis slahmong CK Lim against the pathogenic fungus Sclerotium rolfsii which causes rot disease of the stem base of peanut plants an in vitro.The study consisted of two sub activities: (a) inhibited of colony diameter using Patato Dextrosa Agar (PDA) medium and (b) inhibited of colony biomass using Potato dextrose Broth (PDB) medium, the treatments tested were leaf essential oil and rhizome of wild ginger and fractions A1, B2, C3 and D4, with concentration levels (0, 100, 250 and 500 ppm). Experiments (a) and (b) were arranged in the form of a Completely Randomized Design (CRD) in factorial each with 4 replications. The results showed that the leaf essential oil and rhizome of wild ginger and its fractions had the potential to be used as a vegetable fungicide. The A1 fraction has the best antifungal effectiveness compared to the B2 fraction, leaf oil, rhizomes and other fractions, with the highest inhibition of diameter and biomass of S rolfsii colony the 49.47% and 51.46%. Essential oils of leaves and rhizome oil are not statistically significantly different, but in numerically leaf oil are better than rhizome oil. The C3 fraction showed the lowest colony diameter inhibition and biomass of 34.70% and 36.95%. The best concentration level in inhibition the growth of S rolfsii mushroom is 500 ppm, with inhibition of the diameter and biomass of the colony by 81.74% and 84.25%.

scholarly journals Antibacterial Activity and Chemical Composition of Red Peacock Flower (Caesalpinia pulcherrima L.) Leaf Essential Oil

2019 ◽  
Vol 22 (6) ◽  
pp. 269-274
Author(s):  
Novena Risnalani Rintank Constani ◽  
Hartati Soetjipto ◽  
Sri Hartini
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Gram Positive ◽  
Gram Negative ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Leaf Essential Oil

Peacock flower (Caesalpinia pulcherrima L.) leaves contain essential oils which can be used as an ingredient in cosmetics, perfume, aromatherapy, medicine, and supplements. The study was conducted to obtain essential oils from peacock flower leaves and determine the antibacterial activity against gram-positive bacteria (Bacillus subtilis and Staphylococcus aureus) and gram-negative (Escherichia coli and Pseudomonas aeruginosa). Antibacterial activity test was carried out by the agar diffusion method, using paper discs. Measurements were made for the inhibition zone diameter (IZD) that appeared, while the essential oil component was analyzed using GC-MS. The results showed that the peacock flower leaves (C. pulcherrima) had a moderate to strong antibacterial effect at a concentration of 7.5%-20% against gram-positive bacteria (B. subtilis and S. aureus) and gram-negative bacteria (E. coli and P. aeruginosa). Gram-negative E. coli bacteria are relatively more sensitive to peacock flower leaf essential oil compared to other test bacteria. Peacock flower (C. pulcherrima) leaf essential oil is composed of 7 main components namely β-Cubebene 33.87%; Caryophyllene 23.00%; γ-Elemene 13.18%; α-Pinene 10.96%; Cadina-1(10),4-diene 10.20%; Copaene; 7.09%; β-Pinene 1.70%.

scholarly journals Potensi Antibakteri Ekstrak Bunga Soka (Ixora coccinea L) terhadap Staphylococcus aureus dan Escherichia coli

2016 ◽  
Vol 1 (2) ◽  
pp. 130
Author(s):  
Munira Munira ◽  
Riska Maisarah ◽  
Muhammad Nasir
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Test Results ◽  
E Coli ◽  
Ethanol Extracts ◽  
Ixora Coccinea

Soka plants (Ixora coccineal L.) often used to treat diarrhea, dysentery and wounds. This study was conducted to determine the effect of soka flower ethanol extract in inhibiting the growth of Staphylococcus aureus and Escherichia coli. This study is experimentally using completely randomized design (CRD) with 4 treatments and 6 repetitions which is distilled water as a control, the ethanol extract of soka flowers concentration are 100%, 75%, and 50%. Microbiological test using the diffusion method of Kirby-Baueur. Phytochemical test results soka ethanol extracts of flowers contain alkaloids, flavonoids, tannins, saponins, and triterpenoid. ANOVA test results ethanol extracts of soka flowers greatly affect the growth of S. aureus and E. coli (P=0.000). Duncan test results an average diameter of inhibition zone for the ethanol extract of soka flowers against S. aureus at a concentration of 100% (14.50 mm) was significantly different from the concentration of 75% (10.33 mm) and concentration of 50% (10.67 mm), while the average diameter of inhibition zone for the ethanol extract of soka flowers against E. coli at 100% (11.00 mm) was not significantly different from the 75% (7.83 mm) and 50% (7.50 mm). The ability of ethanol extract of soka flower in inhibition zone against S. aureus greater than E. coli. Keywords:               Ixora coccinea L., inhibition zone, Staphylococcus aureus and Escherichia coli.

UJI AKTIVITAS ANTIBAKTERI KOMBINASI EKSTRAK DAUN SIRIH DAN BIJI PINANG SERTA GAMBIR TERHADAP Streptococcus mutans

2020 ◽  
Vol 5 (2) ◽  
pp. 298-308
Author(s):  
Munira Munira ◽  
◽  
Galuh Trioktafiani ◽  
Muhammad Nasir ◽  
Keyword(s):  
Streptococcus Mutans ◽  
Leaf Extract ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Areca Nut ◽  
Test Results ◽  
Betel Leaf ◽  
Randomized Design ◽  
Antibacterial Potential

Chewing the betel leaf is a proses of concocting a mixture of several ingredients such us betel leaves, areca nuts and gambier were known to provide a refreshing teste, overcoming stress and believed can strengthen teeth and gums. In addition, these plants also suspected of having antibacterial potential. This research was conducted to determine the ability of combination of betel leaf extract, areca nut and gambier against Streptococcus mutans. This research was experimental by using Completely Randomized Design (CRD) with 8 treatments which is P0 (aquadest), P1{combination of betel leaf extract, areca nut and gambier (3:3:3)}, P2 (3:2:3), P3 (3:1:2), P4 (1:2:3), P5 (2:1:3). P6 (1:3:2) dan P7 (2:3:1). Microbiology test using was diffusion method. Based on results of Anova showed that combination of betel leaf extract, areca nut and gambier greatly effect the growth of Streptococcus mutans (P = 0,000). Duncan further test results showed that the largest average diameter of inhibition zone is indicated by P1 which is 29,50 mm and not significantly different from P2 (26,0 mm) and P3 (26,25 mm) but significantly different from P4 (18,25 mm), P5 (21,5 mm), P6 (18,75 mm) and P7 (21,75 mm). While the smallest average diameter of inhibition zone is indicated by P4 (18,24 mm) and not significantly different from P5 (21,50 mm), P6 (18,75 mm) and P7 (21,75 mm) but significantly different from P1 (29,50 mm) and not significantly different from P2 (26,0 mm) and P3 (26,25 mm).

scholarly journals BETEL LEAF ESSENTIAL OIL (Piper betle L.) FOR PEOPLE WITH FREEZING FACTORS BLOOD DISORDERS

2017 ◽  
Vol 6 (3) ◽  
pp. 63
Author(s):  
Teguh Hari Sucipto ◽  
Nourmalasari Aisyah ◽  
Puji Lestari ◽  
Wanda Putri Sacharisa ◽  
Harsasi Setyawati
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Piper Betle ◽  
Test Results ◽  
Blood Disorders ◽  
Betel Leaf ◽  
Liquid Liquid Extraction ◽  
Blood Clots ◽  
Viii And Ix ◽  
Leaf Essential Oil

Betel leaf (Piper betle L.) is a medicinal plant. There betel leaf essential oil content and show a variety of biological activity, including antibacterial. The purpose of this study was to determine the effect of betel leaf essential oil on blood coagulation in patients with factor VIII and IX of blood plasma disorders. Isolation of essential oil made by steam distillation method using two kinds of solvents distilled water and n-hexane. N-hexane extract obtained is separated by liquid-liquid extraction and rotary evaporator. The test results of blood clots increases as the concentration of essential oils, namely essential oils ½ times dilution of 99.67 seconds; dilution ¼ times 127 seconds; dilution 1/8 times 179 seconds; and dilution 1/16 times 242.67 seconds.

Chemical Composition and Antibacterial Activity of Essential Oils of Three Medicinal Plants from Algerian Semi-Arid Climatic Zone

2018 ◽  
Vol 16 (S1) ◽  
pp. S155-S163 ◽  
Author(s):  
S. Mehalaine ◽  
O. Belfadel ◽  
T. Menasria ◽  
A. Messaili
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Rosmarinus Officinalis ◽  
Salvia Officinalis ◽  
Thymus Algeriensis

The present study was carried out to determine, for the first time, the chemical composition and antibacterial activity of essential oils derived from the aerial parts of three aromatic plants Thymus algeriensis Boiss & Reut, Rosmarinus officinalis L., and Salvia officinalis L. growing under semiarid conditions. The essential oils were chemically analyzed and identified by gas chromatography (GC) and GC/ mass spectrometry (GC/MS) and their antimicrobial activity was individually evaluated against Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa using both agar disk diffusion and agar dilution methods. The major constituents of Thymus algeriensis essential oil were identified as camphor (13.62%), 1,8-cineol (6.00%), borneol (5.74%), viridiflorol (4.00%), and linalool (3.93%). For Rosmarinus officinalis essential oil, 48 compounds were characterized, of which the main constituents were camphor (17.09%), Z-β-ocimene (10.88%), isoborneol (9.68%), α-bisabolol (7.89%), and borneol (5.11%). While, Salvia officinalis essential oil was characterized by β-thujone (16.44%), followed by viridiflorol (10.93%), camphor (8.99%), 1,8-cineol (8.11%), trans-caryophyllene (5.85%), and α-humulene (4.69%) as the major components. Notably, results from antibacterial screening indicated that Thymus algeriensis and Salvia officinalis essential oils exhibited a strong inhibitory effect against both Escherichia coli and Staphylococcus aureus compared to Rosmarinus officinalis essential oil. Further, less activity was recorded against Pseudomonas aeruginosa for the three tested essential oils.

scholarly journals Chemical Compositions, Mosquito Larvicidal and Antimicrobial Activities of Essential Oils from Five Species of Cinnamomum Growing Wild in North Central Vietnam

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1303 ◽  
Author(s):  
Do N. Dai ◽  
Nguyen T. Chung ◽  
Le T. Huong ◽  
Nguyen H. Hung ◽  
Dao T.M. Chau ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Larvicidal Activity ◽  
Antimicrobial Agents ◽  
Chemical Compositions ◽  
North Central ◽  
Mosquito Larvicidal Activity ◽  
Central Vietnam ◽  
Leaf Essential Oil

Members of the genus Cinnamomum (Lauraceae) have aromatic volatiles in their leaves and bark and some species are commercially important herbs and spices. In this work, the essential oils from five species of Cinnamomum (C. damhaensis, C. longipetiolatum, C. ovatum, C. polyadelphum and C. tonkinense) growing wild in north central Vietnam were obtained by hydrodistillation, analyzed by gas chromatography and screened for antimicrobial and mosquito larvicidal activity. The leaf essential oil of C. tonkinense, rich in β-phellandrene (23.1%) and linalool (32.2%), showed excellent antimicrobial activity (MIC of 32 μg/mL against Enterococcus faecalis and Candida albicans) and larvicidal activity (24 h LC50 of 17.4 μg/mL on Aedes aegypti and 14.1 μg/mL against Culex quinquefasciatus). Cinnamomum polyadelphum leaf essential oil also showed notable antimicrobial activity against Gram-positive bacteria and mosquito larvicidal activity, attributable to relatively high concentrations of neral (11.7%) and geranial (16.6%). Thus, members of the genus Cinnamomum from Vietnam have shown promise as antimicrobial agents and as potential vector control agents for mosquitoes.

scholarly journals Essential Oils from Indigenous Iranian Plants: A Natural Weapon vs. Multidrug-Resistant Escherichia coli

2022 ◽  
Vol 10 (1) ◽  
pp. 109
Author(s):  
Mohammadreza Pajohi Alamoti ◽  
Behnaz Bazargani-Gilani ◽  
Razzagh Mahmoudi ◽  
Anna Reale ◽  
Babak Pakbin ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Essential Oil ◽  
Essential Oils ◽  
Antimicrobial Properties ◽  
Multidrug Resistant ◽  
Zataria Multiflora ◽  
Cuminum Cyminum ◽  
Plant Essential Oils ◽  
E Coli ◽  
Mentha Longifolia

Aim of this study was to investigate the antimicrobial properties of herbal plant essential oils (EOs) from selected Iranian plant species such as Ferulago angulata, Zataria multiflora, Cuminum cyminum, and Mentha longifolia against antibiotic-resistant Escherichia coli (E. coli) strains. For this purpose, the Escherichia coli strains, isolated from raw cow’s milk and local dairy products (yogurt, cream, whey, cheese, and confectionery products) collected from different areas of Hamedan province, Iran, were investigated for their resistance to antibiotics (i.e., streptomycin, tetracycline, gentamicin, chloramphenicol, ciprofloxacin, and cefixime). Thus, the E. coli strains were tested for their susceptibility to the above-mentioned essential oils. Regarding antibiotics, the E. coli strains were highly sensitive to ciprofloxacin. In relation to essential oils, the most effective antibacterial activity was observed with Zataria multiflora; also, the bacteria were semi-sensitive to Cuminum cyminum and Mentha longifolia essential oils. All strains were resistant to Ferulago angulata essential oil. According to the results, the essential oil of Zataria multiflora can be considered as a practical and alternative antibacterial strategy to inhibit the growth of multidrug-resistant E. coli of dairy origin.

scholarly journals Komposisi Minyak Atsiri dan Aktivitas Antimikroba Rimpang Temu Putih dan Jahe Gajah sebagai Fitobiotik Pakan Unggas

2019 ◽  
Vol 6 (2) ◽  
pp. 181
Author(s):  
Laila Nur Rohma ◽  
Laila Nur Rohma ◽  
Osfar Sjofjan ◽  
M. Halim Natsir
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Essential Oil ◽  
Essential Oils ◽  
Feed Additives ◽  
Diffusion Method ◽  
Disc Diffusion ◽  
Activity Test ◽  
Main Component

ABSTRAK                                                                        Imbuhan pakan unggas dapat berasal dari bahan herbal yang mengandung berbagai komponen aktif yang bermanfaat bagi pertumbuhan ternak.Temu putih dan jahe gajah dapat dimanfaatkan sebagai imbuhan pakan karena mengandung minyak atsiri yang dapat berperan sebagai agen antibakteri. Penelitian ini bertujuan untuk mengetahui komponen penyusun minyak atsiri dan aktivitas antimikroba pada rimpang temu putih dan jahe gajah. Penelitian dilakukan dengan percobaan in vitro menggunakan temu putih dan jahe gajah yang diolah menjadi bentuk ekstrak minyak atsiri temu putih dan jahe gajah sebagai materi uji komposisi penyusun minyak atsiri serta bentuktepung dan enkapsulasi sebagai materi uji aktivitas antimikroba. Komposisi minyak atsiri temu putih terdiri dari lima komponen penyusun dengan cis-1,7-octadien-3-yl acetat sebagai komponen utama. Komposisi minyak atsiri jahe gajah terdiri dari tujuh komponen dan benzene,1-(1,5-dimethyl-4-hexenyl)-4-methyl-(CAS) ar-curcumene sebagai komponen utama. Minyak atsiri yang terkandung pada temu putih dan jahe gajah mempunyai peran dalam menghambat mikroba. Uji komposisi penyusun minyak atsiri menggunakan alat GC-MS dan uji aktivitas antimikroba menggunakan metode disc diffusion dan. Hasil dari uji aktivitas antimikroba menunjukkan bahwa temu putih dan jahe gajah dalam bentuk tepung dan enkapsulasi memiliki perbedaan yang sangat nyata (P<0,01) terhadap aktivitas antimikroba pada bakteri asam laktat, Escherichia coli dan Salmonella sp. Campuran temu putih dan jahe gajah (1:1) menunjukkan kemampuan terbaik dalam menghambat pertumbuhan bakteri patogen dengan diameter zona hambat 5,70±0,14 mm  (Escherichia coli) dan 6,88±0,45 mm (Salmonella sp.).Kata Kunci : antimikroba, fitobiotik, jahe gajah, minyak atsiri, temu putihABSTRACTThe poultry feed additives can contain herbal ingredients that contain various beneficial components for livestock growth. White turmeric and giant ginger can be used as feed additives because they contain essential oils that can be used as antibacterial agents. This study aims to determine the constituent components of essential oils and antimicrobial activity in white turmeric and giant ginger rhizomes. The study was carried out by in vitro experiments using white turmeric and giant ginger which were processed into the form of essential oil extract as material for the composition of essential oils test, and powder and encapsulation form as antimicrobial activity test material. The composition of essential oils of white turmeric consists of five constituent components with cis-1,7-octadien-3-yl acetate as the main component. The composition of giant ginger essential oil consists of seven components with benzene, 1- (1,5-dimethyl-4-hexenyl) -4-methyl- (CAS) ar-curcumene as the main component. Essential oils contained in the white turmeric and giant ginger have a role in inhibiting microbes. The composition of the essential oil tested using GC-MS and the antimicrobial activity test used the disc diffusion method. The results of the antimicrobial activity test showed that white turmeric and giant ginger in powder and encapsulation form had significant differences (P <0.01) on antimicrobial activity in lactic acid bacteria, Escherichia coli and Salmonella sp. The mixture of white turmeric and giant ginger (1: 1) showed the best ability to inhibit the growth of pathogenic bacteria with inhibitory zone diameters of 5.70 ± 0.14 mm (Escherichia coli) and 6.88 ± 0.45 mm (Salmonella sp.).Keywords: antimicrobial, essential oil, giant ginger, phytobiotic, white turmeric

scholarly journals COMBINED EFFECT OF STREPTOMYCIN AND SWEET ORANGE ESSENTIAL OIL TO MEMBRANE AND ACROSOME INTEGRITY BOER GOAT FROZEN SEMEN

2019 ◽  
Vol 3 (2) ◽  
pp. 89
Author(s):  
Sukma Aditya Sitepu ◽  
Julia Marisa
Keyword(s):  
Essential Oil ◽  
Sweet Orange ◽  
Membrane Integrity ◽  
Boer Goat ◽  
Randomized Design ◽  
Frozen Semen ◽  
Acrosome Integrity ◽  
Completely Randomized Design ◽  
Orange Essential Oil

One of factors that cause a bad quality of Boer Goat frozen semen is the growth of bacterial. This can be overcome by adding antibiotics such as streptomycin. To further suppress the growth of bacteria can be added other ingredients that contain antibacterials such as sweet orange essential oil. The purpose of this research is to know the percentage value of Membrane Integrity and Acrosome Integrity on Boer Goat frozen semen with addition sweet orange essential oil and streptomycin. The method used was experimental using Completely Randomized Design with 5 treatments and 5 replications. The treatment in this research is addition 0%, 0,25%; 0.5%; 0.75% and 1% sweet orange essential oil on tris yolk and streptomycin extender. The results showed the best treatment addition combination streptomycin and sweet orange essential oil to percentage Membrane Integrity and Acrosome Integrity is increase 1% sweet orange essential oil.Keywords: Boer Goat, essential oil, frozen semen, streptomycin, sweet orange.

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