This article is devoted to the analysis of business climate of the regions of Ukraine. Important for the analysis are the major changes that the business environment has undergone due to the new realities caused by the spread of the pandemic. Among the key points are: the introduction of a hybrid workflow, digitalization of work processes, development of new crisis management strategies, implementation of sustainable models that can serve customers in any circumstances, retraining and retraining, changing the focus on increasing loyalty services already existing customers, etc. Also, this paper contains an analysis of the business environment in four strategic regions of Ukraine – Lviv, Odessa, Kharkiv and Dnipro. At present, most regions find it difficult to do business in most regions, although satisfactory estimates prevailed last year. Corruption and bureaucracy were highlighted as leading factors hindering business development. In the first place for ease of doing business was the Western region – 2.95 points out of 5 possible, in second place was Kharkiv – 2.69 points, in third place - the Dnieper – 2.34 points, and in last place was the South Ukrainian region – 2.25 points. The integrated assessment of all regions is located in the negative plane. The conclusions listed the main points on which the government should focus for economic recovery and development of the business environment, namely: implementation of real and effective judicial reform, rule of law, fair justice; protection of the population from COVID-19 (large-scale testing, vaccination, effective treatment; eradication of corruption; ensuring fair, equitable, predictable and transparent tax policy, fair competition; ensuring macroeconomic stability and continuing cooperation with the IMF; reducing the shadow economy; protecting investment and property rights; establishing equal rules of the game by eliminating the control of the oligarchs, etc.