scholarly journals Evaluation of Acute Toxicity of Ethanol Extract of Pirdot Leaf (Saurauia vulcani Korth.) in Rats

2020 ◽  
Vol 3 (2) ◽  
pp. 13-18
Author(s):  
Nerly Juli Pranita Simanjuntak ◽  
Rosidah ◽  
Yuandani
Keyword(s):  
Acute Toxicity ◽  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Female Rats ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin

Traditionally pirdot leaves are used to treat various diseases. The purpose of this study was to determine determine the potential for acute toxicity of ethanolic extract of pirdot leaf (Saurauia vulcani Korth.) with value LD50 and hematological Parameters in rats. The acute toxicity of ethanolic extract of pirdot leaf was evaluated by OECD guidelines. The number of animals used in this research were 15 female rats. The control group was given Na CMC 0.5%, the treatment groups were given ethanolic extract of pirdot leaves with doses 2000 and 5000 mg/kg bw. The results showed that ethanolic extract of pirdot leaves with doses of 2000 and 5000 mg/kg bw did not show any toxicity signs. There was no mortality was observe. The ethanolic extract of pirdot leaves did not cause any changes in hematological parameters, these include red blood cells (RBC), hemoglobin, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), platelet, white blood cells (WBC), neutrophils, lymphocytes, monocytes, eosinophils, and basophils levels as compared to normal control (P>0.05). It was estimated that LD50 of ethanolic extract of pirdot leaves was higher than 5000 mg/kg bw and the extract were practically non-toxic. The ethanolic extract of pirdot leaves did not cause any toxic effect on hematological parameters.

scholarly journals Effect of ketorolac administration post bone cavitation surgery on some hematological parameters

2017 ◽  
Vol 41 (1) ◽  
pp. 169-177
Author(s):  
Raffal A. Omar
Keyword(s):  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Treated Group ◽  
Control Group ◽  
Mean Corpuscular Hemoglobin ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Post Operation

     This study was designed to determine the effect of ketorolac on some hematological parameters post cavitation surgery in domestic rabbits. Ninety-six adult domestic local breed male rabbits were used in this study, weighing (1.23 ± 0.33) kg. They were divided randomly into two groups in the first one (n= 48/group) which represented control group, while the second one (n=48/group) represented the treated group (Ketorolac group). All rabbits in both groups undergoes surgical operation (bone cavitation in femur bone) under general anesthesia. In the Ketorolac group, animals were treated by using 30 mg/kg of Ketorolac directly post-operation and continued daily for 5 days, while in Control group no treatment was given postoperatively. The hematological parameters were recorded which include white blood cells count; red blood cells count; hemoglobin; packed cell volume; mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration; mean corpuscular volume and platelets at the period of (3, 5, 7, 14, 21, 28, 35, and 42) days post operation were estimated. Results concerning hematological values showed no significant differences in packed cell volume values between control and treated group and within each group, while haemoglobin values showed a significant decrease at period 7 and 14 days between control and treated group. Within control group the significant decrease were noticed clearly at period of 7 days. Data concerning mean corpuscular hemoglobin values within control group showed significant decrease were noticed at 5 day, but within treated group, there were no significant differences. mean corpuscular hemoglobin concentration values showed significant decrease ate period 3; 5 and 21 days between control group and treated group respectively. Within control group the significant decrease were noticed obviously at 5 days while within treated group the significant decrease were noticed at 35 days. mean corpuscular volume values showed significant increase at 3 and 5 days period between control and treated groups. Platelets counts within control group showed significant increase at period of 14 days, within treated group the significant increase were recorded at 42 days. Red blood cells showed significant decrease between control and treated groups, while within control group, the significant decrease were at 7 days. White blood cells count within treated group showed significant increase recorded at 21 days. Differential White blood cells count concerning Lymphocytes cells within control group the significant increase were recorded at 42 days  while the significant decrease were noticed at 21 days, but within treated group the significant increase were recorded at 35 days  and significant decrease were noticed at 14 days. Neutrophils counts showed significant decrease at periods 3, 14, 28 and 42 days between control and treated groups respectively; while within control group there were significant decrease at 3 days, but within treated group the significant decrease were noticed at 42 days. Monocytes cells showed significant increase at 35 days between control and treated groups, within control group there were significant increase at 35 days, while within treated group the significant increase recorded at 7 days.

scholarly journals Evaluation of Toxic Effect of Phaleria macrocarpa (Scheff.) Boerl Leaf Extract on Hematological Parameters

1970 ◽  
Vol 8 (3) ◽  
pp. 01-04
Author(s):  
Hanif M Q ◽  
Yuandani Y ◽  
Harahap U
Keyword(s):  
Toxic Effect ◽  
Blood Cells ◽  
Leaf Extract ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Ethanol Extract ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Phaleria Macrocarpa ◽  
Hematology Parameters

Objectives: This current study was conducted to evaluate the toxic effect of P. macrocarpa leaf extract (Phaleria macrocarpa (Scheff.) Boerl) on hematology parameters in rats. Design: The toxicity of ethanol extract of P. macrocarpa leaf was evaluated by OECD guidelines. The extract at doses of 100, 500, 1000 mg/kg body weight (bw), control satellite and satellite group dose 1000 mg/kg bw were orally administered to the test animal for 90 days. Hematological parameters were observed for 90 days and 118 days for treatment and satellite group, respectively. Interventions: The variable that was intervened in this study was the doses of P. macrocarpa extract. Main outcome measure: The main results in this study were the toxic effect of P. macrocarpa leaf on hematology parameters. Results: The etanol extract of P. macrocarpa did not cause any changes in hematological parameters, these include red blood cells (RBC), hemoglobin, hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), platelet, white blood cells (WBC), neutrophils, lymphocytes, monocytes, eosinophils, and basophils levels as compared to normal control (P>0.05) Conclusion: The ethanol extract of P. macrocarpa leaf did not cause any toxic effect on hematological parameters for long-term use.  

scholarly journals Hematological parameters of Iranian cichlid Iranocichla hormuzensis: Coad, 1982 (Perciformes) in Mehran River

2012 ◽  
Vol 84 (4) ◽  
pp. 943-949 ◽  
Author(s):  
Ehsan Daneshvar ◽  
Mahsa Y. Ardestani ◽  
Salar Dorafshan ◽  
Mauricio L. Martins
Keyword(s):  
Blood Cells ◽  
Mean Corpuscular Volume ◽  
High Efficiency ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Food Fish

This study describes the hematological parameters in Iranocichla hormuzensis, an Iranian freshwater cichlid important as ornamental and food fish. Forty fish were captured with seine net at Mehran river Hormozgan province, Iran. Blood was used to determine the total counts of red blood cells (RBC) and white blood cells (WBC), hematocrit, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC) and morphometric data of erythrocytes. The Iranian fish showed lower RBC and WBC values than the other cichlids (Oreochromis niloticus, O. aureus, O. mossambicus, O. hybrid, Cichlasoma dimerus and Cichla monoculus). Hematocrit did not vary among the species, but MCV, MCH and MCHC in I. hormuzensis were higher than those for O. niloticus, O. aureus, O. hybrid, C. dimerus and C. monoculus. These differences may be related to different life habit of fish. This study suggests that I. hormuzensis is well acclimated to the environment being the first report for its hematology. It is also suggested high efficiency in oxygen transportation, and an efficient inflow of oxygen by the gills, indicating the welfare of fish on this environment.

Effect of gender on hematological parameters in growing Ouled Djellal sheep of semi arid zones of Northeastern Algeria

2021 ◽  
Vol 11 (4) ◽  
pp. 407-417
Author(s):  
Zerari Aicha ◽  
Mahdi Djahida ◽  
Allaoua Sofia Amel ◽  
Rouar Salim
Keyword(s):  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Arid Zones ◽  
Mean Corpuscular Hemoglobin Concentration ◽  
Corpuscular Hemoglobin ◽  
Mean Values ◽  
Ewe Lambs

Ouled Djellal sheep is the most dominant breed in Algeria due to its adapta-tion to harsh steppe environment. Hematological parameters are good indi-cators of animal health, but their values are breed-specific, and they are affected by age and gender. Therefore, this study aimed to determine the hematological profile of healthy Ouled Djellal lambs and ewe lambs reared traditionally under semi-arid zones of Algeria during the growing peri-od. Blood samples were taken from twenty-four Ouled Djellal lambs (12 males, 12 females) at 42, 63, 84 and 105 days of age to evaluate the count of White Blood Cells, Lymphocyte, Monocyte, Granulocyte, Red Blood Cell and total Platelet, the percentage of Lymphocyte, Monocyte and Granulocyte, Hemoglobin concentration, Hematocrit, Mean Corpuscular Volume, Mean Corpuscular Hemoglobin and Mean Corpuscular Hemoglobin Concentration by usual known methods. Both male and female lambs did not show signifi-cant variations in mean values of White Blood Cells, Lymphocyte count, Red Blood Cells, Hemoglobin, Mean Corpuscular Hemoglobin concentration, per-centage of Monocyte, Monocyte count, Gran percentage and Granulocyte count from 42ͭ ͪ day to 105ͭ ͪ days. Mean values of percentage of Lymphocyte, Hematocrite in lambs, Mean Corpuscular Hemoglobin in ewe lambs, and Mean Corpuscular Volume, Platelets levels in both lambs and ewe lambs indicated also variations during this period of growth. Significant Pearson's correlations were found between some hematological parameters in Ouled Djellal lambs and/or ewe lambs. Therefore, these findings provide usual val-ues, which can be considered for assessing the physiological, nutritional and health status of these animals.

scholarly journals The effects of Nigella sativa oil administration on some physiological and histological values of reproductive aspects of rats

2011 ◽  
Vol 35 (2) ◽  
pp. 52-60
Author(s):  
Farooq T. Juma
Keyword(s):  
Blood Cells ◽  
Nigella Sativa ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Female Rats ◽  
Cholesterol Concentration ◽  
Control Group ◽  
Sexual Hormones ◽  
Histological Changes ◽  
Accessory Glands

The goal of this study to investigate the effects of Nigella sativa (Ns) oil on reproductive values, some hematological parameters serum biochemical characteristics, some sexual hormones concentration and histological changes of treated and normal male reproductive organs. The experiment (1) dealt with 20 males and 20 female rats at 21 days of age, 10 rats of each sex were giving orally Ns oil at the rate of 1ml/kg/day for 30 days and the others left as a control group. Insignificant changes were occurred in hematological parameters except the white blood cells (WBCs), were significantly increased in treated groups (P<0.05). The treated groups showed significant increases in total protein and significant decreases in total cholesterol liver enzymes markedly increased in treated rats Significant increases in the levels of LH, FSH and testosterone for males and LH, FSH, estrogen and progesterone for females were recorded. The experiment (2) dealt with effects of Ns oil on 20 adult males and 20 adult female rats that were given same dose of Ns oil for 30 days. There were significant increase in litter size and weight of rats born in treated groups. The experiment (3) dealt with the effects of Ns oil on castrated males fifteen adult rats were divided equally into 3 groups, two groups were castrated while, the third group was left as a control group; one castrated group was treated with same dose of Ns oil for 30 days, there were significant increases in serum testosterone concentration and weight of accessory glands in treated group. Histological changes in the accessory glands of treated groups were evident.In conclusion, the administration of 1ml/kg/day of Ns oil stimulated the secretion of sexual hormones that led to improve protein synthesis of hepatic enzymes, white blood cells count and decrease the serum cholesterol concentration in blood

scholarly journals Investigation of hematological parameters related to the severity of COVID-19 disease in Mashhad, Iran

2020 ◽  
Vol 7 (2) ◽  
pp. e19-e19
Author(s):  
Mohsen Seddigh-Shamsi ◽  
Shima Nabavi Mahali ◽  
Mahnaz Mozdourian ◽  
Abolghasem Allahyari ◽  
Neda Saeedian ◽  
...  
Keyword(s):  
Blood Cells ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Polymerase Chain Reaction Test ◽  
Healthcare Personnel ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Distribution Width ◽  
Volume Number ◽  
Time Of Admission

Introduction:Human coronavirus disease 2019 (COVID-19) is an emerging respiratory disease that has become pandemic worldwide. The patients have a range of mild to severe symptoms, such as pneumonia, acute respiratory distress syndrome, arrhythmia, shock, and other organs involvement. Clinical and hematological evidence can serve as a prognostic factor to detect severe cases of COVID-19. Objectives: The present study aimed to evaluate hematological parameters related to the severity of the disease in COVID-19 patients in Mashhad, Eastern Iran. Patients and Methods: All the individuals with positive real-time reverse transcription-polymerase chain reaction test results and the patients with clinical symptoms whose computed tomography scan results were matched with the diagnosis of viral pneumonia were included in the study. The hematological examinations and complete blood count of the patients were performed by the healthcare personnel through collecting blood samples at the time of admission and discharge from the hospital. Results: In total, 189 COVID-19 patients were included in the study, 47.6% and 58.73% of whom were over 60 years old and male, respectively. Investigation of the hematological parameters revealed that the number of white blood cells, mean value of corpuscular volume, number of platelets, and lymph count had significantly increased at the time of discharge (P<0.01). On the other hand, red blood cells, hemoglobin, hematocrit, mean corpuscular hemoglobin, mean corpuscular hemoglobin, and red cell distribution width values were significantly higher at the time of admission in comparison to discharge (P<0.01). Furthermore, it was found that male gender had a significant relationship with the development of lymphopenia in the patients (P=0.021). Conclusion: It can be concluded that hematological parameters and inflammatory biomarkers in COVID-19 patients could potentially act as the independent factors and affect the prognosis of the disease.

scholarly journals Changes in hematological parameters and gill morphology in common carp exposed to sub-lethal concentrations of Malathion

2016 ◽  
Vol 2 (3) ◽  
pp. 370-378 ◽  
Author(s):  
Sadia Sharmin ◽  
Md Abdus Salam ◽  
Farhana Haque ◽  
Md Sadiqul Islam ◽  
Md Shahjahan
Keyword(s):  
Common Carp ◽  
Blood Cells ◽  
Morphological Changes ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Blood Parameters ◽  
Mean Corpuscular Hemoglobin ◽  
Agricultural Field ◽  
Corpuscular Hemoglobin

Malathion is an organophosphorous insecticide widely used in the agricultural field in Bangladesh and reach in the aquatic environment through rain wash. In the present study, we examined the effects of Malathion on hematological parameters and gills morphology in common carp exposed to two sub-lethal concentrations (1.5 and 3.0 mg/L) for a period of 192h (8 days). For hematological parameters fish were sacrificed at 24, 48, 72, and 96h after start of exposure. Gills were collected at 192h after start of exposure. The blood glucose level was significantly elevated with increasing the concentration of Malathion. Red blood cells (RBCs), hemoglobin (Hb) and hematocrit (Hct) values were significantly decreased after the exposure of Malathion. Significant increments were observed in case of the mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC), while no distinct changes were noted for mean corpuscular hemoglobin (MCH). Interestingly, white blood cells (WBCs) count was significantly increased with toxicity of Malathion. Several morphological changes, such as telangiectasia, blood lamellar congestion, hypertrophy of filaments, lamellar fusion were observed in the gills of fish exposed to Malathion. The present study revealed that the insecticide had adverse effects on various blood parameters and gills morphology in common carp. Thus, the use of insecticide in the agriculture field may be a threat to fauna and flora of the aquatic environment.Asian J. Med. Biol. Res. September 2016, 2(3): 370-378

scholarly journals AMELIORATIVE ROLE OF BEE HONEY AND ROYAL JELLY AGAINST CISPLATIN INDUCED ALTERATION IN HEMATOLOGICAL PARAMETERS IN MALE WISTER ALBINO RAT

2018 ◽  
Vol 10 (4) ◽  
pp. 110 ◽  
Author(s):  
Waykar Bhalchandra ◽  
Yahya Ali Alqadhi ◽  
A. S. Ninawe
Keyword(s):  
Blood Cells ◽  
Royal Jelly ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Control Group ◽  
Corpuscular Volume ◽  
Corpuscular Hemoglobin ◽  
Mean Values

Objective: This study aims to investigate the ameliorative role of dietary bee honey and royal jelly against cisplatin-induced alterations in hematological parameters in male wistar albino rat.Methods: Male wistar albino rats of same age and weight were randomly divided into four groups; G, I: control group which was given 0.9% saline, G: II: cisplatin (7 mg/kg/d) was injected intraperitoneally for 15 d, G, III bee honey with royal jelly (500 mg/kg/d of honey and 100 mg/kg/d of royal jelly) fed orally daily for 15 d, G, IV: cisplatin (7 mg/kg/d) was injected intraperitoneally and honey (500 mg/kg/d) and royal Jelly (100 mg/kg/d) fed orally daily for 15 d. The hematological parameters like total number of white blood cells (WBCs), red blood cells (RBCs), platelets, % of hemoglobin (Hb), and mean values of packed cell volume (PCV), mean corpuscular volume (MCV) and mean corpuscular hemoglobin concentration (MCHC) were measured by using automated hematology system.Results: Cisplatin treated rats revealed a significant decrease in total number of white blood cells (WBCs), red blood cells (R. B. Cs), platelets, percentage of hemoglobin (Hb), and mean values of packed cell volume (PCV), corpuscular volume (MCV) and corpuscular hemoglobin concentration (MCHC) as compared to control group. Royal jelly and honey treated group of rats revealed a significant increase in all blood parameters compared to control group. Dietary bee honey with royal jelly along with cisplatin-treated rats revealed significant increas as compared to animals treated with cisplatin (G, II) and the computed significant valus for the above parameters are 10.00, 2.30, 8,54, 12.00, 35.00, 47.40 and 32,30 respectively.Conclusion: Bee honey and royal jelly could be used as dietary preventive natural products against cisplatin-induced hematological alterations during the treatment of cancer.

scholarly journals CLINICOPATHOLOGICAL EFFECTS OF HEAT STRESS ON BLOOD PICTURE OF JAPANESE QUAILS

2021 ◽  
Vol 9 (5) ◽  
pp. 913-916
Author(s):  
Khurshaid Anwar ◽  
◽  
Nakash Smark ◽  
Sartaj Khan ◽  
Kalim Ullah ◽  
...  
Keyword(s):  
Heat Stress ◽  
Blood Cells ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Coturnix Japonica ◽  
Mean Corpuscular Hemoglobin ◽  
Coturnix Coturnix Japonica ◽  
Corpuscular Hemoglobin ◽  
Coturnix Coturnix ◽  
Japanese Quails

This study aimed to evaluate the effects of heat stress on quail hematological parameters two published papers on quails in 2013 were studied, analyzed and compared. Broiler Japanese quails (Coturnix coturnix japonica) were exposed to high ambient temperature (340C, 8hr/d, 0900-1700hr) in both papers. An increase in values of red blood cells count, white blood cells count, lymphocytes, heterophils and lymphocytes (H/L) ratio, Monocytes, packed cell volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration were recorded while a lower values of heterophils, basophils, eosinophils, mean corpuscular volume and hemoglobin concentration than normal were recorded in groups exposed to heat stress (340C) on day 42 in Japanese quails (Coturnix coturnix japonica). Hemoglobin concentration was least affected. The obtained results indicated that, rearing quail birds under high environmental conditions during the fattening period has a great adverse effect on blood parameters. To help maintain Japanese quail as an essential animal model further heat stress related research on modern ways is needed.

scholarly journals Statin and anemia in chronic kidney disease (CKD): an experimental study

2018 ◽  
Vol 197 ◽  
pp. 07003 ◽  
Author(s):  
P N Cahyawati ◽  
A A S A Aryastuti ◽  
M B T Ariawan ◽  
N Arfian ◽  
Ngatidjan Ngatidjan
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Hematological Parameters ◽  
Treatment Options ◽  
White Blood Cells ◽  
Hemoglobin Concentration ◽  
Mean Corpuscular Hemoglobin ◽  
Corpuscular Hemoglobin ◽  
Simvastatin Group ◽  
Group 5

Anemia is a common feature of chronic kidney disease (CKD). The current management of patients with anemia in CKD is controversial. These make anemia is one of the leading causes poor outcome in CKD patients. Our study focus to assess the effect of statin on hemoglobin level with an experimental model of CKD. We perform the animal model of CKD by subtotal nephrectomy procedure. A total of 20 male swiss was used in these study. All the animal divided into 4 groups: sham group (S), nephrectomy (Nx), simvastatin group 5 mg/kgBB (SV5), and simvastatin group 10 mg/kgBB (SV10). Hematological parameters (hemoglobin (Hb), white blood cells (WBC), red blood cell (RBC), hematocrit (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC)), and serum creatinine are measured at the end of the study. The hemoglobin levels in the Ux group decreased significantly compared with the S group (11.02 ± 0.46 vs 14.3 ± 0.15, p<0.0001). While in the SV group, statin administration led to an increase in hemoglobin levels compared with the Ux group but not statistically significant (SV5: 13.26±0.34; Sv10: 12.68±0.26). These results suggest that administration of statins in CKD may improve the condition of anemia by an unknown mechanism, while still requiring other treatment options to maximize the effect of therapy.

Sign in / Sign up

Export Citation Format

Share Document